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  • 2B - J310200061 - Garnis Annisa A.M - Analisis Kadar Gula Reduksi

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    Saminur Fauzan
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    The document presents the results of an experiment to determine glucose content through spectrophotometry. It includes a table of absorbance measurements for glucose solutions with varying concentrations. It then shows the calculations to determine the linear regression equation relating absorbance (Y) to concentration (X). This allows calculating the concentration of an unknown sample based on its absorbance. The regression analysis finds the equation to be Y = 5.2714X + 0.0167. Applying this to the unknown sample gives its concentration as 0.051 grams of glucose per liter, or 25.5% glucose content.

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    2B_J310200061_Garnis Annisa A.M_Analisis Kadar Gula Reduksi

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    The document presents the results of an experiment to determine glucose content through spectrophotometry. It includes a table of absorbance measurements for glucose solutions with varying concentrations. It then shows the calculations to determine the linear regression equation relating absorbance (Y) to concentration (X). This allows calculating the concentration of an unknown sample based on its absorbance. The regression analysis finds the equation to be Y = 5.2714X + 0.0167. Applying this to the unknown sample gives its concentration as 0.051 grams of glucose per liter, or 25.5% glucose content.

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    © All Rights Reserved
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    6 views3 pages

    2B - J310200061 - Garnis Annisa A.M - Analisis Kadar Gula Reduksi

    Uploaded by

    Saminur Fauzan
    The document presents the results of an experiment to determine glucose content through spectrophotometry. It includes a table of absorbance measurements for glucose solutions with varying concentrations. It then shows the calculations to determine the linear regression equation relating absorbance (Y) to concentration (X). This allows calculating the concentration of an unknown sample based on its absorbance. The regression analysis finds the equation to be Y = 5.2714X + 0.0167. Applying this to the unknown sample gives its concentration as 0.051 grams of glucose per liter, or 25.5% glucose content.

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    © All Rights Reserved
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    of 3

    E.

    Hasil dan Pembahasan

    1. Tabel Hasil Perhitungan

    X (kadar
    Tabung Y (Absorbansi) X2 Y2 XY
    glukosa)
    1 0 0 0 0 0
    2 0,02 0,134 0,0004 0,017956 0,00268
    3 0,04 0,236 0,0016 0,055696 0,00944
    4 0,06 0,328 0,0036 0,107584 0,01968
    5 0,08 0,460 0,0064 0,2116 0,0368
    6 0,1 0,524 0,01 0,274576 0,0524
    N 0,3 1,682 0,022 0,667412 0,121

    2. Perhitungan

    Diketahui :

    - Σ X (kadar glukosa) = 0,3

    - Σ Y (Absorbansi) = 1,682

    - Σ X2 = 0,022

    - Σ Y2 = 0,667412

    - Σ XY = 0,121

    - n =6

    - FP = 5000

    - V = 1 ml

    - Berat Sampel = 1000 mg


    Ditanya :

    a. b...?

    b. a...?

    c. Y...?

    d. X...?

    e. Kadar Gula Reduksi (%)...?

    f. Kurva...?

    Jawab :

    -
    a. b =
    -

    -
    =
    -

    -
    =
    -

    = = 5,2714

    -
    b. a =

    -
    =

    -
    =

    = = 0,0167
    c. Y = bx + a

    = 5,2714x + 0,0167

    d. Nilai Y (Kel. 10) = 0,286

    -
    X =

    -
    = = 0,051

    e. Kadar Gula Reduksi (%) = X 100 %

    = X 100 %

    = 25,5 %

    f. Grafik Kurva Standar

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