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  • A202001002 - Andi Nuning

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    sasya
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    The primer picking results provide 4 potential primer pairs for a 273 base pair template sequence. The best pair has a left primer starting at position 99 and a right primer at position 270, with predicted melting temperatures of 59.24C and 59C respectively and a product size of 172 base pairs. In total 1044 left primers and 1044 right primers were considered.

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    A202001002_ANDI NUNING

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    The primer picking results provide 4 potential primer pairs for a 273 base pair template sequence. The best pair has a left primer starting at position 99 and a right primer at position 270, with predicted melting temperatures of 59.24C and 59C respectively and a product size of 172 base pairs. In total 1044 left primers and 1044 right primers were considered.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    2 views1 page

    A202001002 - Andi Nuning

    Uploaded by

    sasya
    The primer picking results provide 4 potential primer pairs for a 273 base pair template sequence. The best pair has a left primer starting at position 99 and a right primer at position 270, with predicted melting temperatures of 59.24C and 59C respectively and a product size of 172 base pairs. In total 1044 left primers and 1044 right primers were considered.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    7/17/23, 9:14 AM Primer3 Output (primer3_results.cgi release 4.1.

    0)

    Primer3 Output

    PRIMER PICKING RESULTS FOR

    Template masking not selected


    No mispriming library specified
    Using 1-based sequence positions
    OLIGO start len tm gc% any_th 3'_th hairpin seq
    LEFT PRIMER 99 20 59.24 60.00 1.32 0.00 0.00 GGTGGGTATAGCTCAGGTGG
    RIGHT PRIMER 270 20 59.00 50.00 0.00 0.00 0.00 AGTGGATCAAATGGCTGGGA
    SEQUENCE SIZE: 273
    INCLUDED REGION SIZE: 273

    PRODUCT SIZE: 172, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    1 TCACCCGCTCCAATAAATGCGTCTTTAGCTCAGCTGGATAGAGCAACGGCCTTCTAAGCC

    61 GTGTGCCAGGGGTTCGACTCCCTTAAGACGCACCATATGGTGGGTATAGCTCAGGTGGTA
    >>>>>>>>>>>>>>>>>>>>

    121 GAGCGCCAGATTGTGGTTCTGGATGCCAAGGGTTCGAGTCCCTTTATCCACCCTAGTATA

    181 ATAATGGGATGTCGCCAAGTGGTAAGGCACGGGACTTTGACTTCCGCATTCGTAGGTTCG

    241 AATCCTGCCATCCCAGCCATTTGATCCACTAGC
    <<<<<<<<<<<<<<<<<<<<

    KEYS (in order of precedence):


    >>>>>> left primer
    <<<<<< right primer

    ADDITIONAL OLIGOS
    start len tm gc% any_th 3'_th hairpin seq

    1 LEFT PRIMER 1 20 58.61 50.00 0.00 0.00 0.00 TCACCCGCTCCAATAAATGC


    RIGHT PRIMER 222 20 58.95 50.00 0.00 0.00 0.00 AGTCAAAGTCCCGTGCCTTA
    PRODUCT SIZE: 222, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    2 LEFT PRIMER 77 20 58.72 50.00 3.36 0.00 0.00 ACTCCCTTAAGACGCACCAT


    RIGHT PRIMER 266 20 59.32 55.00 0.00 0.00 0.00 GATCAAATGGCTGGGATGGC
    PRODUCT SIZE: 190, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    3 LEFT PRIMER 75 19 57.97 57.89 3.36 0.00 0.00 CGACTCCCTTAAGACGCAC


    RIGHT PRIMER 230 20 58.47 50.00 0.00 0.00 0.00 AATGCGGAAGTCAAAGTCCC
    PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    4 LEFT PRIMER 101 21 58.04 52.38 1.32 0.00 0.00 TGGGTATAGCTCAGGTGGTAG


    RIGHT PRIMER 256 20 59.82 55.00 0.00 0.00 0.00 CTGGGATGGCAGGATTCGAA
    PRODUCT SIZE: 156, PAIR ANY_TH COMPL: 0.00, PAIR 3'_TH COMPL: 0.00

    Statistics
    con too in in not no tm tm high high high high
    sid many tar excl ok bad GC too too any_th 3'_th hair- poly end
    ered Ns get reg reg GC% clamp low high compl compl pin X stab ok
    Left 1044 0 0 0 0 14 0 346 275 10 1 113 0 0 285
    Right 1044 0 0 0 0 10 0 327 241 0 0 12 0 0 454
    Pair Stats:
    considered 6995, unacceptable product size 6990, primer in pair overlaps a primer in a better pair 2140, ok 5
    libprimer3 release 2.4.0

    (primer3_results.cgi release 4.1.0)

    https://primer3.ut.ee/cgi-bin/primer3/primer3web_results.cgi 1/1

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