GelPilot ® DNA Molecular Weight Markers

For easy and accurate sizing of DNA fragments

bp bp bp bp
– 1500 – 10,000
– 8000
– 500 – 1000 7000
– 400 – 900 – 6000
– 600 – 800 – 5000
– 350 – 700
– 300 – 500 4000
– 400 – 600
– 250 – 3000
– 500
– 200 – 300
– 150 – 400
– 200 – 2000
– 100 – 300
2.5% agarose

0.5% agarose
2.5% agarose

2.0% agarose
– 100
– 50 – 200

– 100 – 1000

GelPilot 50 bp GelPilot 100 bp GelPilot 100 bp Plus GelPilot 1 kb

Ladder (100) Ladder (100) Ladder (100) Ladder (100)
(cat. no. 239025) (cat. no. 239035) (cat. no. 239045) (cat. no. 239085)

bp bp bp bp
– 15,000
– 10,000 – 10,000
– 8000 – 7500
– 5000 – 4500 – 2000 – 5000
– 3000
– 1000 – 2500
– 2000 – 2000
– 1600 – 750
– 1200
– 1000 – 500
– 800 – 1000
– 700
– 500 – 500 – 250
– 400
1.0% agarose

1.0% agarose

0.8% agarose
2.0% agarose

– 300
– 200 – 100
– 100 – 200
– 250

GelPilot 1 kb GelPilot Wide Range GelPilot Mid Range GelPilot High Range
Plus Ladder (100) Ladder (100) Ladder (100) Ladder (100)
(cat. no. 239095) (cat. no. 239125) (cat. no. 239135) (cat. no. 239145)

Product description
Benchtop versions of QIAGEN GelPilot DNA Molecular Weight
Markers combine many useful features, including memorable fragment
sizes, ready-to-load buffer, defined fragment concentrations for
accurate DNA quantification as well as convenient room-temperature
storage for up to 6 months. The 8 ready-to-use markers cover a wide
range of fragment sizes, guaranteeing an ideal format for every
requirement. GelPilot buffer contains 3 tracking dyes (xylene cyanol,
bromophenol blue, and orange G) to facilitate the optimization of
agarose gel run time. Each marker provides 100 x 6 ıul gel loadings.

Sample & Assay Technologies

Procedure
1. Mix the ladder gently by pipetting up and down a few times. Directly load 6 ıul of the required
ladder (this will provide 50 ng per DNA fragment with one intense fragment of 100 ng).
2. Load your DNA samples (using a suitable loading buffer) and apply current to the gel.
3. Use the tracking dyes to optimize the gel run time and DNA resolution. DNA can be visualized
using ethidium bromide or SYBR® Green reagent under UV light.

Storage conditions
GelPilot DNA Molecular Weight Markers can be stored at room temperature (15–25°C) for
up to 6 months or at 2–8°C for up to 12 months in a constant - temperature fridge. For longer
storage, store at –20°C in a constant - temperature freezer. Preparing aliquots minimizes the risk
of contamination.

Safety information
When working with chemicals, always wear a suitable lab coat, disposable gloves,
and protective goggles. For more information, please consult the appropriate material safety
data sheets (MSDSs). These are available online in convenient and compact PDF format
at www.qiagen.com/support/MSDS.aspx where you can find, view, and print the MSDS
for each QIAGEN kit and kit component.

24-hour emergency information

Emergency medical information in English, French, and German can be obtained 24 hours
a day from:
Poison Information Center Mainz, Germany
Tel: +49-6131-19240

GelPilot DNA Molecular Weight Markers are intended for molecular biology applications.
These products are neither intended for the diagnosis, prevention, or treatment of a disease,
nor have they been validated for such use either alone or in combination with other products.

Trademarks: QIAGEN®, GelPilot® (QIAGEN Group) ; SYBR® (Molecular Probes, Inc.).

1056212 02/2009 © 2008 QIAGEN, all rights reserved.

www.qiagen.com Canada I 800-572-9613 Ireland I 1800 555 049 Norway I 800-18859

China I 0086-21-3865-3865 Italy I 800 787980 Singapore I 65-67775366
Denmark I 80-885945 Japan I 03-6890-7300 Spain I 91-630-7050
Australia I 1-800-243-800 Finland I 0800-914416 Korea (South) I 1544 7145 Sweden I 020-790282
Austria I 0800/281010 France I 01-60-920-926 Luxembourg I 8002 2076 Switzerland I 055-254-22-11
Belgium I 0800-79612 Germany I 02103-29-12000 Mexico I 01-800-7742-639 UK I 01293-422-911
Brazil I 0800-557779 Hong Kong I 800 933 965 The Netherlands I 0800 0229592 USA I 800- 426- 8157

Sample & Assay Technologies