FCM-MICROBIOLOGY

SPONTANEOUS GENERATION VS. Franz Schulze and

BIOGENESIS
Theodor Schwann
Lazzaro Spallanzani
➢ Recreate Needham’s
experiment Schulze
Results ➢ Passed air through strong
acid solution into boiled
➢ Broth remained clear
broth
Schwann
SG Supporters
➢ Through the red hot tube
➢ The air was required for the
vital force to work. Results
➢ No microbes
SG Supporters
➢ Strong acid and heat altered
the air so it cannot support
the growth
Georg Friedrich Schroder Louis Pasteur (1822-1895)
and Theoder von Dusch ➢ Father Of Modern
(1850) Microbiology
➢ Filtered air through sterile ➢ Place nutrient solution in
cotton wool the flask
➢ created flask with long
Result curved necks
➢ No microbial growth ➢ boiled the solutions
➢ left flask exposed to air
Result
➢ no microbial growth
The defeat of spontaneous
generation: Pasteur’s swan-
necked flask experiment
John Tyndall (1820-1893) Ferdinand Cohn
➢ Demonstrated that dust ➢ Discovered the existence of
carries microorganisms heat-resistant bacterial
➢ Provided evidence for the endospores
existence of exceptionally
heat-resistant forms of Malachite green
bacteria ➢ Used as a green-coloured
➢ Tyndallization dye, as a counter-stain in
histology, and for its anti-
o also called fractional fungal properties in
sterilization aquaculture.
o is a form of * Cell has a negative charge
sterilization that
involves boiling the - phosphate group
goods to be sterilized
in their cans or jars
at 100 degrees
Centigrade for about 15
to 20 minutes a day,
for three days in a
row.
o Not reliable in wine
preservation
Ignaz Semmelweis (1861) Robert Koch (1843 to 1910)

➢ Asepsis(practices that kill ➢ Establish the relationship

some microorganisms to between the bacillus
prevent them from anthracis and anthrax
spreading)in obstetrical ➢ Koch’s postulate
wards to prevent the o set of criteria for
transmission of childbirth proving that a given
fever (refers to an microorganism causes a
infection of the given disease
reproductive organs several
days after delivering a
baby, including urinary
tract infections) from
patient to patient
➢ Policy for all attending
physicians to wash their
hands with chloride of
lime between patients.

Joseph Lister (1867)

➢ Father of Antiseptic Surgery

➢ Provided indirect direction
for the importance of
microbes in causing
diseases.
➢ Used phenol and carbolic
acid in surgical dressings,
and heat sterilized surgical
instruments.