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CARMONA NATIONAL HIGH SCHOOL

Carmona, Cavite

Special Science Program

LYCOPENE EVALUATION OF TOMATOES (Solanum Lycopersicum)

BASED ON WATER ANALYSIS

A Research Proposal

Presented to

Carmona National High School

in Partial Fulfillment

of the Requirements in

Special Science Program

Datiles, Jacob Elijah C.

Leonardo, Gilyan Oona R.

Reunada, AJ Kean B.

NOVEMBER 2022
CARMONA NATIONAL HIGH SCHOOL
Carmona, Cavite

Special Science Program

APPROVAL SHEET

This research entitled “LYCOPENE EVALUATION OF TOMATOES


(Solanum Lycopersicum) BASED ON WATER ANALYSIS ”, prepared
and submitted by Jacob Elijah C. Datiles, Gilyan Oona R.
Leonardo, and AJ Kean B. Reunada, in partial fulfillment
of the requirements in Special Science Program has been
examined and is recommended for ORAL DEFENSE.

KEVIN C. TAGUIBAN
Adviser

CERTIFICATE OF APPROVAL
PANEL OF EXAMINERS
Approved by the committee on oral defense on 23 February
2023 with the grade of 8.

Chairman

Member Member

Accepted and approved in partial fulfillment of the


requirements in Special Science Program.

BRENDA ESTEBAN
Science Head Department

JOSE E. SAMSON JR.


Principal IV
CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

TABLE OF CONTENTS

Introduction

Statement of the Problem

Hypotheses

Methodologies

Research Design

Population and Sampling

Research Locale

Materials

Data Gathering Procedure

Gantt Chart

Budget Proposal

Bibliography

Appendices

Appendix A Intel ISEF Forms

Curriculum Vitae
CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

LIST OF TABLES

Table 1. List of materials and quantities for tomato

cultivation

Table 2. Gantt chart for Research Manuscript

Table 3. Gantt chart for Sample Collection. Proper Care

of Tomatoes, Harvesting, and Data Collection.

Table 4. The Total Cost and Suppliers of the research


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

LIST OF FIGURES

Figure 1. Flowchart of research methodology


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

PROBLEM AND ITS BACKGROUND

The Philippines confronts serious problems

concerning water quality, accessibility, water shortage,

and temporal distribution. According to World Health

Organization (WHO), over 3 million Filipinos still rely

on unsafe and unsustainable water sources and 7 million

lack improved sanitation. The importance of water in

agriculture cannot also be overstated. It is essential

that crops and livestock have access to sufficient amounts

of clean, usable water. Water is not only useful for

agriculture, but also for maintaining the environment in

sensitive or protected areas, such as wetlands. Access to

clean water for drinking and performing basic hygiene at

home, in schools, and in healthcare facilities is limited

by a shortage of water. Water scarcity can cause sewage

systems to malfunction and an increase in the risk of

getting diseases like cholera. Water that is hard to

obtain also costs more. There are several factors that

affect the availability of freshwater, including overuse,

increasing demand, pollution, poor management, inadequate

infrastructure, and changing weather patterns due to

global warming.
CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

There are various sources of water that are used to

water plants to address this issue. Water analysis of

different sources like grey water can be tested if this

will affect the nutrients we get from the plants.

Greywater is household wastewater generated from the reuse

of shower, laundry, and hand sink water. After treatment,

this water can be used to irrigate a garden. Greywater can

be treated and recycled to nourish the soil, which in turn

produces lush, healthy plants because it contains

nutrients from soap and other residues (McCloy 2019). The

wastewater that would otherwise go into the septic and

sewer system is used to create greywater, which may

eventually end up in water bodies. Utilizing greywater

helps reduce this type of pollution because the wastewater

can then contaminate the water bodies. In this project,

the researchers will test whether greywater could have an

impact on phytochemicals. As defined by ScienceDirect,

phytochemicals are chemical substances that plants make,

usually to aid in their resistance to diseases by fungi,

bacteria, and plant viruses. The primary phytochemical in

tomatoes, lycopene, is responsible for most of the fruit's

health advantages. Lycopene protects against cancer by

activating phase II enzymes, which protect against cancer.

Tomato contains many other phytochemicals, mainly


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

carotenoids, and flavanols, which have antioxidant

activity and which in combination with lycopene may

contribute to the health benefits of the tomato. The

tomato, or Solanum Lycopersicum, is a flowering

solanaceous plant grown for its edible fruit. Tomatoes are

a good source of phytochemical lycopene and vitamin C. The

fruit is often eaten fresh in salads, cooked as a

vegetable, added to a variety of prepared foods, and

pickled. In addition, much of the world's tomato

production is processed into products such as tomato

juice, ketchup, puree, paste, and "sun-dried" or dried

pulp (Britannica, 2022).

To keep life on Earth, water conservation is

immediately required. There will come a day when the

survival of the Homo sapiens species will be in jeopardy

as water becomes more scarce. People must cultivate

behaviors that prioritize water conservation. By reusing

greywater, people can minimize their overall water

consumption and help protect the environment. A greywater

system can be helpful in many ways. These include

maintaining your garden and replenishing the natural water

table. Research in South Brazil has found that most of

these benefits fall into one of three categories:


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

Installing a greywater system can save you up to 42% pure

water. Additionally, recycling this water benefits the

entire neighborhood by easing pressure on the public water

supply.
CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

STATEMENT OF THE PROBLEM

The research aims to investigate the impact of various

types of water on tomato plants. Some possible problem

statements that could be addressed are:

1. Does the quality of water used to irrigate tomatoes

affect the amount of lycopene produced in the fruit?

2. What is the impact of using greywater versus tap

water on lycopene levels in tomatoes?

3. Can the use of organic fertilizers combined with

specific types of water increase the production of

lycopene in tomatoes?

4. How does the presence of other minerals and chemicals

in water affect the lycopene content of tomatoes?

5. Is there a significant difference in the lycopene

levels of tomatoes grown with different types of

water and fertilizers?


CARMONA NATIONAL HIGH SCHOOL
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HYPOTHESIS

This study aims to investigate the potential effects

of different water sources on the lycopene content of

tomato plants. In order to achieve this, three hypotheses

will be tested.:

1. Null hypothesis (Ho): The type of water used

(greywater, tapwater) does not affect the lycopene

content of tomatoes.

Alternative hypothesis (Ha): The type of water

used (greywater, tapwater) affects the lycopene

content of tomatoes.

2. Null hypothesis (Ho): There is no significant

difference in lycopene content between tomatoes

grown with greywater and those grown with

tapwater.

Alternative hypothesis (Ha): There is a

significant difference in lycopene content between

tomatoes grown with greywater and those grown with

tapwater.

3. Null hypothesis (Ho): Water type has no effect on

the yield of tomato plants.


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

Alternative hypothesis (Ha): Water type affects

the yield of tomato plants.


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

METHODOLOGIES

Research Design

This study is conducted using an experimental design.

Various factors such as growing conditions, harvest times,

and processing methods can influence the lycopene effects

of tomatoes in this design. Using this type of research

design, causality can be established between variables.

Population and Sampling

For the experiment aimed at studying the effects of

different water types on tomato plants, the researchers

will use eight plants, four of which will be placed inside

and four outside. The greywater used in the experiment

will be classified into three categories: bathroom

greywater, dish greywater, and laundry greywater. One

tomato plant will be assigned to each greywater category,

while the fourth plant will be used for tap water. It is

also important to note that the population for this study

could consist of all ripe tomatoes of a given variety

cultivated under specific conditions, such as those grown

in a particular location or using a specific growing

method. A subset of ripe tomatoes, such as those

cultivated under certain conditions or at a specific time


CARMONA NATIONAL HIGH SCHOOL
Carmona, Cavite

Special Science Program

of year, could also be included in the population. The

sample size and procedure for this study will be

determined by the specific research question and

population. For example, if the population consists of all

ripe tomatoes of a given type cultivated in a specific

location, a simple random sample from the population might

be collected. A stratified or cluster sample may be more

suited if the population is a subset of ripe tomatoes,

such as those cultivated under certain conditions or at a

specific time of year.

Research Locale

The study will be conducted in the residence of one

of the researchers that gives the researchers access to

the materials and equipment required for the research. The

research location should also be safe and secure, and any

potential ethical or legal concerns should be considered.

Ethical Consideration

To do this experiment, the following considerations

would be followed:

• Participants should be informed of the purpose of

the study, the methods used, and the potential

risks and benefits. They should be given the


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

opportunity to ask questions and provide informed

consent prior to participating in the experiment.

• The study should be designed and executed in a

manner that minimizes harm to participants. This

includes taking steps to minimize any physical,

psychological, or social harm that may result from

participating in the study.

• Participants should have the right to withdraw from

the study at any time without penalty. They should

also be provided with any information they need to

make informed decisions about their participation.

• The data collected during the study should be used

only for the purpose for which it was collected and

not disclosed or used for any other purposes

without the consent of the participants.

Research Disposal

Proper disposal of waste materials is an important

aspect of any research project. This ensures that the

environment is protected and that the waste does not pose

a risk to human health. In this research, waste materials

that will be generated include excess greywater, used

soil, and metal wires. As such, it is important to have a

disposal plan that is both environmentally friendly and


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

safe. This plan will involve recycling where possible, as

well as proper disposal methods for any waste that cannot

be recycled.

1. Excess greywater: Greywater can be reused for non-

potable purposes such as flushing toilets and

cleaning. If it cannot be reused, it should be

disposed of in a designated greywater system or a

septic system. It should not be poured onto the

ground, as it can contaminate soil and groundwater.

2. Used Soil: It can be reused in gardening or

landscaping projects or composted. Composting is an

eco-friendly method of recycling organic materials,

which can be used to enrich soil.

3. Metal wire: Metal wire can be recycled at a recycling

center or scrap yard. It should not be disposed of

in regular trash or mixed with other recyclables.


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

Research Instruments and Techniques

The objective of this study is to find out how

different types of water affect the growth and development

of tomato plants. The study seeks to determine which type

of water (greywater or tapwater) benefits tomato growth

the most in terms of plant height, number of tomatoes

produced, plant weight, and overall plant health. This

data will be collected through a controlled experiment in

which all other variables, such as fertilizer and

sunlight, remain constant while only the type of water

changes. The findings of this study will help to improve

understanding of sustainable irrigation strategies for

small-scale food crop production, as well as the possible

use of greywater in agriculture.

In making the tomatoes, researchers will use the

following materials and factors:

• Tomato seeds - (dependent variable)

• Water – Greywater and Tapwater (independent variable)

• 10 L Water Gallon – will be served as a pot that

allows the researchers to develop more changeable

beds. (Controlled variable)


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

• Loam Soil (with Organic Fertilizer) - provides the

necessary support (controlled variable)

• Sunlight - helps the plant to produce tomatoes

(Controlled variable)

• Scissors/Hand Pruners - to cut the stalk.

• Watering can - to water the tomato plants with the

different water types, ensuring each plant receives

the same amount of water.

• Shovel - to dig holes for planting the tomato

seedlings in the soil.

• Drill - to make holes in the 10L water gallon to

allow for controlled pouring of the water during the

experiment.

• Metal wire – will be used to make climbing supports

for the tomatoes.

Additional:

• 4 in 1 Soil pH meter - to measure the moisture,

temperature, acidity, and sunlight intensity of the

soil in which the tomatoes are grown.

• Water testing kit - to measure the pH, total

dissolved solids (TDS), and conductivity of the

different types of water.


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

These additional materials will help the

researchers better control and measure the variables in

their study and gather more comprehensive data on the

effect of different types of water on tomato growth.

Table 1. List of materials and quantities for tomato


cultivation

MATERIALS AND EQUIPMENT: QUANTITY


Tomato Seeds 24 pcs.
Water (Greywater & Tapwater) 250 L
Recycled 10 L water gallon. 8 pcs.
Loam Soil with Organic Fertilizer 8 bags.

Scissors/Hand pruners 1 pc.


4 in 1 Soil pH meter 1 pc.
Digital Water Testing kit 1 pc.
Metal wire (for Climbing Support) 20 m
Watering Can 1 pc.
Shovel 1 pc.
Drill 1 pc.
CARMONA NATIONAL HIGH SCHOOL
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METHODS:

Observational Studies: involve observing and collecting

data on tomatoes, their lycopene content, and other

factors that may affect lycopene content, such as growing

conditions and processing methods.

Experiments: are a type of research design that involves

manipulating one or more independent variables and

observing the effects on a dependent variable. In this

research, experiments could be used to examine the effect

of different types of water on the lycopene content of

tomatoes.

Laboratory Tests: Laboratory tests, such as

spectrophotometry or high-performance liquid

chromatography (HPLC), can be used to measure the lycopene

content of tomatoes and other food samples.

Data Analysis: Statistical analysis, such as regression

analysis or analysis of variance (ANOVA), can be used to

analyze and interpret the data collected from the research

methods.
CARMONA NATIONAL HIGH SCHOOL
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DATA GATHERING PROCEDURES

1 Sample Collection

1.1 Tomatoes

The researchers will collect a representative sample of

tomatoes for analysis. This can be done by selecting a

random sample of plants from a larger population or by

growing tomatoes under controlled conditions.

1.2 Greywater

A variety of sources can produce greywater, including

bathtubs, shower stalls, washbasins in the bathroom,

dishes, and washing machines. Ensure that the collected

greywater is stored in a clean, covered container to

prevent contamination. It is important to test greywater

for chemical and biological contaminants before using it

for irrigation. With the water testing kit, you can test

the pH and the total dissolved solids (TDS). Depending on

the results, greywater can be used for irrigation if it

is within acceptable limits.


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1.3 Tapwater

The sample could be drawn from a tap in the researcher's

residence or laboratory. The sample should be handled in

the same manner as the greywater samples.

2 Proper Care of Tomatoes

2.1 The Experimental Set-up

This will include preparing the pots, soil, and seeds.

The researchers may decide to use a specific type of pot

and soil that is suitable for tomato growth and provides

the necessary nutrients.

2.2 Watering

The researchers will water the tomato plants 1-2 liters

of water per week (143ml to 286ml per day), following the

recommended water requirements for tomato plants. The

water used for irrigation will be either greywater or tap

water depending on the study's objective.


CARMONA NATIONAL HIGH SCHOOL
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2.3 Monitoring

The researchers will monitor the growth and development

of the tomato plants regularly. This will include

observations of the plant's height, stem diameter, number

of leaves, and fruit production.

3 Irrigation Used

The irrigation methods used in this research can be

either:

3.1 Overhead Irrigation

Overhead irrigation is a method of watering plants in

which water is sprayed from above, typically with a hose

or watering can. Water can be applied uniformly to the

entire plant bed or targeted to individual plants or

regions that require watering. This technique is

frequently used to water outside plants, lawns, and

gardens.
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3.2 Hand Irrigation

Hand Irrigation, on the other hand, is a more accurate

method in which water is administered directly to the soil

around the plant using a watering can or a hose with a

nozzle. This method provides greater control over the

amount of water given to each plant, making it easier to

water specific plants or regions that require additional

attention. Hand Irrigation is frequently used for indoor

plants, seedlings, and small outdoor plants that require

frequent watering.

4 Harvesting The Tomatoes

4.1 Determine the appropriate time for Harvest.

The researchers should know when the tomatoes are ready

for harvest, usually when the fruits are fully mature and

have reached their maximum size (The tomato plant usually

matures in 3 months).

4.2 Recording the Data

The researchers should record the date, time, and

conditions (e.g. temperature, humidity) at the time of


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

harvest, as well as the number and weight of the tomatoes

collected from each plant.

4.3 Label and store the Samples.

The researchers should properly label and store the

harvested tomatoes, taking care to prevent contamination

and preserve the quality of the samples.

5 Data Collection

5.1 Observations

This involves observing and recording various aspects

of the tomato plants, such as growth, yield, water usage,

and nutrient uptake. This can be done using tools such as

a ruler, digital scale, and thermometer.

5.2 Water Analysis

This involves analyzing the chemical and physical

properties of the different types of water used for

irrigation. This can be done using standard laboratory

procedures and equipment, such as spectrophotometers and

pH meters.
CARMONA NATIONAL HIGH SCHOOL
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5.3 Data analysis

This involves analyzing the data collected from the

various methods to determine the impact of different water

types on tomato plants. This can be done using statistical

software and models to identify trends, correlations, and

causal relationships between variables.

5.4 Record keeping

This involves keeping detailed records of all data

collected, including observations, samples, and results

from experiments and analysis. This helps to ensure

accuracy, consistency, and completeness of the data, and

allows for future replication and verification of the

research findings.
CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

FLOWCHART

Figure 1. Flowchart of the research methodology


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GANTT CHART

Table 2. Gantt chart for Research Manuscript

Table 3. Gantt chart for Sample Collection. Proper Care of


Tomatoes, Harvesting, and Data Collection.
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BUDGET PROPOSAL

Table 4. The Total Cost and Suppliers of the research

UNIT TOTAL
# ITEMS QUANTITY UNIT SUPPLIERS
COST COST
YIERYI SVG KRL'S
1 Tomato Seeds 24 PC ₱0.00 ₱0.00
Water
2 (Greywater & 250 L ₱0.00 ₱0.00
Tapwater)

3 Recycled 10 L 8 PC ₱0.00 ₱0.00


water gallon.
Loam Soil
4 with Organic 8 BAG ₱60.00 ₱480.00
Fertilizer

5 Scissors/Hand 1 PC ₱0.00 ₱0.00


pruners
4 in 1 Soil
6 1 PC ₱700.00 ₱700.00
pH meter

7 Digital Water 1 PC ₱400.00 ₱400.00


Testing kit

Metal wire
8 (for Climbing 4 KG ₱80.00 ₱320.00
Support)
Others:
Shovel,
9 1 PC ₱0.00 ₱0.00
Watering Can,
Drill
10 HPLC TEST 8 - - -
OTHER
11 ₱2,000.00
MISCELLANEOUS
TOTAL: ₱3,900.00

*Some items have no expenses or suppliers because the

researchers either already have them or they will use

recycled items.
CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

BIBLIOGRAPHY

Britannica, The Editors of Encyclopaedia. "tomato".


Encyclopedia Britannica, 6 Sep. 2022,
https://www.britannica.com/plant/tomato.

Gardenuity. "Harvesting Tomatoes: The Complete Guide."


https://blog.gardenuity.com/harvesting-tomatoes/.
May 30, 2020.

Tomato Dirt. “Growing Tomatoes Made Easy.” Accessed


February 19, 2023.
https://www.tomatodirt.com/growing-tomatoes.html.

California State Water Resources Control Board. “Greywater


Information.” Accessed February 19, 2023.
https://www.waterboards.ca.gov/water_issues/program
s/greywater_registry/.

Greywater Action. “What Is Greywater?” Accessed February


19, 2023. https://greywateraction.org/.

Oregon Health & Science University. “Greywater Reuse.”


Accessed February 19, 2023.
https://www.ohsu.edu/sustainability/greywater-
reuse.

Water Wise Group. “Greywater Guidelines for Home Gardens.”


Accessed February 19, 2023.
https://www.waterwisegroup.com/greywater-
education/.

American Heart Association. “Lycopene May Lower Stroke


Risk in Men.” Accessed February 19, 2023.
https://www.heart.org/en/news/2018/06/27/lycopene-
may-lower-stroke-risk-in-men.
CARMONA NATIONAL HIGH SCHOOL
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Harvard Health Publishing. “Lycopene-Rich Tomatoes Linked


to Lower Stroke Risk.” Accessed February 19, 2023.
https://www.health.harvard.edu/staying-
healthy/lycopene-rich-tomatoes-linked-to-lower-
stroke-risk

Medical News Today. “What Are the Health Benefits of


Lycopene?” Accessed February 19, 2023.
https://www.medicalnewstoday.com/articles/279484.

National Center for Biotechnology Information. “Lycopene:


Its Properties and Relationship to Human Health.”
Accessed February 19, 2023.
https://ods.od.nih.gov/factsheets/Lycopene-
HealthProfessional/.

Today’s Dietitian. “Lycopene: A Review of Its Potential


as an Antioxidant and Beneficial Molecule.” Accessed
February 19, 2023.
https://www.todaysdietitian.com/newarchives/1119p36
.shtml.
CARMONA NATIONAL HIGH SCHOOL
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CURRICULUM VITAE

STUDENT RESEARCHER 1

PERSONAL INFORMATION

NAME: Jacob Elijah C. Datiles

BIRTHDATE: Sept. 3, 2009

BIRTHPLACE: San Pedro, Laguna

PERMANENT ADDRESS: Blk 5 Lot 19 Phase 10 Carmona

Estates, Carmona Cavite

EMAIL ADDRESS: datilesjacob@gmail.com

EDUCATIONAL QUALIFICATIONS

GRADE LEVEL COMPLETE: Elementary

CURRENT GRADE LEVEL: 8

SECTION: Science Class

SCHOOL: Carmona National High School


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

HONORS AND AWARDS RECEIVED:

ELEMENTARY: With Honors

GRADE 7: With Honors


CARMONA NATIONAL HIGH SCHOOL
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Special Science Program

STUDENT RESEARCHER 2

PERSONAL INFORMATION

NAME: Gilyan Oona R. Leonardo


BIRTHDATE: May 8, 2009
BIRTHPLACE: Alabang, Muntinlupa

PERMANENT ADDRESS: Blk 22 Lot 31 Cedar 3 Carmona


Estates, Carmona Cavite
EMAIL ADDRESS: gilyan.oona@gmail.com

EDUCATIONAL QUALIFICATIONS

GRADE LEVEL COMPLETE: Elementary

CURRENT GRADE LEVEL: 8

SECTION: Science Class

SCHOOL: Carmona National High School


CARMONA NATIONAL HIGH SCHOOL
Carmona, Cavite

Special Science Program

HONORS AND AWARDS RECEIVED:

GRADE 8: Champion ESP Digital Arts

GRADE 7: Top 3 Digital Arts, Top 1 in Science Quiz


Bee
GRADE 6: Champion in English Editorial Cartooning
(DSPC 2021), With Honors
GRADE 5: Top 6 in English Editorial Cartooning
(DSPC)
GRADE 4: Top 11 in English Editorial Cartooning
(DSPC)
GRADE 3: Deportment Award

GRADE 2: Rank 4 in Filipino Quiz Bee (DSPC),

Participant in Filipino Essay Writing


(RSPC)
CARMONA NATIONAL HIGH SCHOOL
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STUDENT RESEARCHER 3

PERSONAL INFORMATION

NAME: AJ Kean B. Reunada

BIRTHDATE: November 29 2009

BIRTHPLACE:

PERMANENT ADDRESS: 10100 San Pablo St. Cabilang

Baybay, Carmona Cavite

EMAIL ADDRESS: ajkeanreunada@gmail.com

EDUCATIONAL QUALIFICATIONS

GRADE LEVEL COMPLETE: Elementary

CURRENT GRADE LEVEL: 8

SECTION: Science Class

SCHOOL: Carmona National High School

HONORS AND AWARDS RECEIVED:


CARMONA NATIONAL HIGH SCHOOL
Carmona, Cavite

Special Science Program

GRADE 5: 1st Place Math Quiz Bee

GRADE 4: 3rd Place (Partners) in Math Division

GRADE 3: 3rd Place (Partners)in Math Division

GRADE 2: Champion (Solos)in Math Challenge

Division

GRADE 1: 7th place (Trio) Math Division


CARMONA NATIONAL HIGH SCHOOL
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APPENDIX A INTEL ISEF FORMS

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