Procan Electronics Inc.

5/F., 4thBldg.,NO.2,
st
Gaoxin C. 1 Ave.,
High-tech Industrial Park,
Nanshan,Shenzhen 518057, P.R. China
Tel: (+86) 755 26516667, 26952867
Fax: (+86) 755 26747910
 Contents

CONTENTS

CHAPTER 1 INSTRUMENT INTRODUCTION ......................................................... ................. 1

1.1 STRUCTURE .......................................................................................................................... 1

1.1.1 Host .......................................................................................................................................1
1.1.2 Accessory ..............................................................................................................................1
1.2 PURPOSE................................................................................................................................2
1.3 SPECIFICATIONS.................................................................................................................. 3
1.4 STRUCTURE .......................................................................................................................... 7
1.4.1 Front Panel.............................................................................................................................7
1.4.2 Rear panel..............................................................................................................................7
1.5 OPERATION ........................................................................................................................... 9
1.5.1 Display Screen.......................................................................................................................9
1.5.2 Menu......................................................................................................................................9
1.6 DETECTION PRINCIPL ...................................................................................................... 12
1.6.1 Detection Principles of WBC, RBC, PLT............................................................................12
1.6.2 Principles of HGB Measurement.........................................................................................13
1.6.3 Volume Distribution of Blood Cell......................................................................................13
1.6.4 Results and Calculation of Parameter Measurement ...........................................................14

CHAPTER 2 HARDWARE.................. ................................................................ .......................... 16

2.1 CPU BOARD.........................................................................................................................16

2.1.1 Function...............................................................................................................................16
2.1.2 Interface...............................................................................................................................17
2.2 CCU BOARD ........................................................................................................................ 17
2.2.1 Function...............................................................................................................................17
2.2.2 Front Interface ..................................................................................................................... 17
2.3 TFT CABLE CONVERTING BOARD ..........................................................................................19
2.3.1 Function...............................................................................................................................19
2.3.2 Interface...............................................................................................................................19
2.4. LCD ADOPTER..............................................................................................................................20
2.4.1. Function ....................................................................................................................................20
2.5 RECORDER BOARD.................................................................................................................. 21
2.5.1 Function .....................................................................................................................................21
2.6 DOM CARD ...................................................................................................................................21
2.6.1 Function .....................................................................................................................................21

CHAPTER 3 TUBING.................................................. ............................................................... ..... 22

3.1 TUBING GRAPH.........................................................................................................................22

3.2 STRUCTURE OF TUBING SYSTEM..................................................................................23
3.2.1 Liquid road structure..................................................................................................................23
3.2.2 Tubing Sensor Unit..............................................................................................................23
3.2.3 HGB Unit.............................................................................................................................24
3.2.4 Dilute Unit...........................................................................................................................24

T- 6807-30-02357 V1.0 I
 Contents

3.2.5 Vacuum Unit........................................................................................................................24

3.2.6 Auxiliary Unit......................................................................................................................24

CHAPTER 4 SYSTEM STRUCTURE............................................................................. ............... 25

4.1 DISASSEMBLE/REPLACEPARTSAND COMPONENTS.......................................................25

4.1.1 Disassemble/Replace the Sample Machine................................................................................25
4.1.2 Disassemble/Replace the Diluent Machine .........................................................................26
4.1.3 Disassemble/Replace the Vacuum Block............................................................................. 26
4.1.4 Disassemble/Replace the Vacuum Pump Block...................................................................27
4.1.5 Disassemble/Replace the Piston Block................................................................................28
4.1.6 Disassemble/Replace Chamber............................................................................................29

CHAPTER 5 ADJUSTMENT .............................................................. ............................................... 31

5.1 GENERAL.............................................................................................................................31
5.2 ADJUST PROGRAM ............................................................................................................ 31
5.3 ADJUST PLACE ................................................................................................................... 32

CHAPTER 6 MAINTENANCE.............. ............................................................... .......................... 33

6.1 ROUTINE MAINTENANCE................................................................................................33

6.1.1 Clean the Appearance of the Instrument..............................................................................33
6.1.2 Replace Fuse........................................................................................................................34
6.2 DAILYMAINTENANCE ..................................................................................................... 34
6.3 WEEKLYMAINTENANCE................................................................................................. 34

6.4 MONTHLYMAINTENANCE..............................................................................................35
6.5 YEARLYMAINTENANCE..................................................................................................35
6.6 THE MAINTENANCE BEFORE TRANSPORTOR FOR INSTRUMENT THAT
WILL NOT BE USED FOR A LONG TIME......................................................................................35

CHAPTER 7 TROUBLESHOOTING ................................................................ .......................... 37

7.1 PROGRAM TEST ........................................................................................................................ 37

7.2 CHECKING ITEMS BEFORE MAINTENANCE ................................................................38
7.3 BACKGROUND CHECK.....................................................................................................38

CHAPTER 8 APPENDIX 1 LEAD LABEL................................................................................... .... 41

CHAPTER 9 APPENDIX 2 DEBUG RECORD FORM ..................................................................42

CHAPTER 10 APPENDIX 3 TROUBLESHOOTING ...................................................... ............... 45

II T- 6807-30-02357 V1.0
 Instrument Introduction

Chapter 1 INSTRUMENT INTRODUCTION

1.1 STRUCTURE

Host and accessory.

1.1.1 Host

Control the process of sample collection, dilution and analysis, including the following units:

Hematology analyzer

Former cover parts RBC chamber WBC chamber Switch parts

CCU board CPU Board System software Backboard parts

Vacuum pump machine Vacuum pump Power supply Transformer

block

Dilution machine Dilution block Glass bulb Liquid tubing

Sample machine TFT cable Ada ter PCBA Li uid Sensor

1.1.2 Accessory

Including the following accessories:

1. PS/2 mouse. PS/2 keyboard.

2. Diluent cap components, washing liquid cap components, hemolytic agent cap components,

1
 Instrument Introduction

waste cap components;

3. PS232 Communication cable;

4. Waste barrels;

5. Printer (Optional ), Bar code scanner (Optional )

1.2 PURPOSE

It is used for detecting the parameters of RBC, WBC, HGB and differential counter.

The analyzer is used for the determination of the following 20 parameters and 3 histograms blood

specimens in Table 1-1.

Item Abbreviation Unit

9
White blood cell or leukocyte WBC 10 /L
9
Lymphocyte LYM# 10 /L
9
Mid-sized cell MID# 10 /L
9
Granulocyte GRAN# 10 /L
Lymphocyte percentage LYM% %
Mid-sized cell percentage MID% %
Granulocyte percentage GRAN% %
12
Red Blood Cell or erythrocyte RBC 10 /L
Hemoglobin Concentration HGB g/L
Hematocrit HCT %
Mean Corpuscular(erythrocyte) Volume MCV fL
Mean Cell Hemoglobin MCH pg
Mean Cell Hemoglobin Concentration MCHC g/L
Red Blood Cell Distribution Width- Standard
RDW-SD fL
Deviation
Red Blood Cell(erythrocyte) Distribution
RDW-CV %
Width Coefficient of Variation
9
Platelet PLT 10 /L
Mean Platelet Volume MPV fL
Platelet Distribution Width PDW %
Plateletcrit PCT %
Plateletcrit-large Cell Ratio P-LCR %
White Blood Cell Histogram WBC Histogram
Red Blood Cell Histogram RBC Histogram
Platelet Histogram PLT Histogram
Table 1-1

2
 Instrument Introduction

1.3 SPECIFICATIONS

 Standard Classification of the Instrument

According to anti-electrical shock hazard: Grade II, Pollution 2

According to the defence for deleterious liquid: General Device (Closing device of

non-defensive liquid)

According to the recommended disinfection/sterilization method of the manufactory:

Disinfectant device recommended by manufactory.

According to safety degree in condition that using gas mixture of flammable

anesthetic gas and air or gas mixture of oxygen and nitrous oxide: Do not use the

equipment in condition that using gas mixture of flammable anesthetic gas and air or

gas mixture of oxygen and nitrous oxide.

According to working status: Continuous Running Equipment.

 Principles of Measurement

Blood Cells are counted and sized by Electrical Impedance Method. Hemoglobin is

determined by Colorimetry Method.

 Parameters of Measurement

Basic parameters:

Item Abbreviation Unit

9
White blood cell WBC 10 /L
12
Red Blood Cell RBC 10 /L

Hemoglobin Concentration HGB g/L

9
Platelet PLT 10 /L

Derived from Histograms:

Item Abbreviation Unit

Lymphocyte percentage LYM% %

Mid-sized cell percentage MID% %

Granulocyte percentage GRAN% %

3
 Instrument Introduction

Mean Corpuscular(erythrocyte) Volume MCV fL

Red Blood Cell Distribution Width Standard RDW-SD fL

Deviation

Red Blood Cell(erythrocyte) Distribution RDW-CV %

Width Coefficient of Variation

Mean Platelet Volume MPV fL

Platelet Distribution Width PDW %

Plateletcrit-large Cell Ratio P-LCR %

Calculated Parameters:

Item Abbreviation Unit

9
Lymphocyte LYM# 10 /L
9
Mid-sized cell MID# 10 /L
9
Granulocyte GRAN# 10 /L

Hematocrit HCT %

Mean Cell Hemoglobin MCH pg

Mean Cell Hemoglobin Concentration MCHC g/L

Plateletcrit PCT %

 Sampling Features:

Venous and Capillary Blood Mode:WBC/HGB 1:300

RBC/PLT 1:44600

Prediluted Mode: WBC/HGB 1:355

RBC/PLT 1:44500

Cell Counting Aperture Size:

WBC/RBC: 80μm

 Display

Liquid Crystal Display (LCD),resolution:640×480

 Language

4
 Instrument Introduction

English

 Indicator

20 Parameters and 3 Histograms Display

Alarm Indicator

Working Status (including Power Supply) Indicator Light

Alarm

 Interface

One power input socket

One printer port

One VGA display port

Two RS232/C serial ports

Two USB interfaces

Two PS/2 interfaces

 Recorder

Rapid Thermal Recorder

Recording width: 48mm

Paper width: 57.5mm

 Cable adapter board, Liquid Sensor, Voltage

When the liquid reagent is in the sensor the voltage is 3.0V ~ 5.0V;

When the liquid reagent is not in the sensor the voltage is ≤1.5V。

 Pre-dilution draining capacity

Pre-dilution draining capacity range should be 8.0±0.4ml

 Aperture voltage reference value

WBC Aperture voltage reference value ：the 5 times average W.H.V.S value of blank test

RBC Aperture voltage reference value：the 5 times average R.H.V.S value of blank test.
 Aperture voltage

Press T1 voltage range 9-20V

Press T2 voltage range less than 3V

 Chamber cover and frame.

 Take electrode cable from CCU board and use the two pens of multimeter (which

5
 Instrument Introduction

have been set the resistance value) to measure the Chamber shield and substrate

Insulation. The value should be more than 20MΩ。When you finish test, return the

electrode cable back. The value should less than 1Ω。

 Screen Display and the Ranges for Report Output Parameters

Parameter Parameter Range Parameter Parameter Range

9 9
WBC 0.0 - 99.9x10 /L GRAN# 0 - 99.9x10 /L
12
RBC 0.00 - 9.99x10 /L HCT 0.0 - 100.0%

HGB 00.0 - 300g/L MCH 0.0 - 999.9pg

9
PLT 0 - 3000x10 /L MCHC 0.0 - 999.9g/L

MCV 0 - 250fL RDW-SD 0.0 - 99.9 fL

LYM% 0 - 100% RDW-CV 0.0 - 99.9%

MID% 0 - 100% PDW 0.0 - 30.0%

GRAN% 0 - 100% MPV 0.0 - 30.0fL

9
LYM# 0 - 99.9x10 /L PCT 0.0 - 9.99%
9
MID# 0 - 99.9x10 /L P-LCR 0.0-99.9%

 Operating Ambient

Temperature: 15℃~35℃

Humidity: 10~90%

Atmospheric Pressure: 86.0kPa ~106.0kPa

 Ambient Storage

Temperature: -10℃~40℃

Humidity: ≤80%

Atmospheric Pressure: 50.0kPa ~106.0kPa

 Electric Specifications

Power Supply: AC 100-240V, 50±1Hz,

The maximum input consumption is 150VA

Fuse: AC 250V 2A

6
 Instrument Introduction

 Dimensions and Weight

Dimensions: 394mm×381mm×500mm

Weight: 23kg

1.4 STRUCTURE

1.4.1 Front Panel

1 5 4 3 2

Figure 1-1

1. Power Light 2. [START] Key

3. Sample Needle 4.Display Screen

5. Thermal Recorder

1.4.2 Rear panel

7
 Instrument Introduction

（9）
（5）

（4）

（6）
（2）
（7）
（3）
（8）

（1）
（18）

（17）

（16）

（14） （12） （11）（10）

（15） （13）

chart 1-2

1. Equipotentiality 2. PS/2 Keyboard Interface

3. PS/2 Mouse Interface 4. Printer Interface

5. USB Serial Interface 6.VGA Display Interface

7. RS-232 Serial Port 1 8.RS-232 Serial Port 2

9. Air Filter 10. Power Switch

11. Socket for Power Input 12. Fuse Holder

13. RINSE Connector 14. DILUENT Connector

15. WASTE Connector 16.WASTE Sensor Connector

17. LYSE Connector 18. Air Filter

8
 Instrument Introduction

1.5 OPERATION

Designed according to human engineering and transferring information to users by software

windows and mouse, both are convenient for your operating.

1.5.1 Display Screen

The display screen is classified five sections:

SYSTEM TIME INFORMATION SHORTCUT KEY

SECTION SECTION SECTION

WINDOWS DISPLAY SECTION

AUXILIARY OPERATION SECTION

Figure 1-3

 SYSTEM TIME SECTION

Display the current date and time of the system.

 INFORMATION SECTION

Display prompt information of the system.

 SHORTCUT KEY SECTION

To display the function interfaces of the system.

 WINDOWS DISPLAY SECTION

Display various results.

 AUXILIARY OPERATION SECTION

Display the information of user.

1.5.2 Menu

You can operate the instrument by menu. Press the right key of the mouse, directing to the

function menu of the current window. Move the mouse to the required menu bar, and then press

the left key of the mouse, the required function will be selected. Pressing the left key out of the

9
 Instrument Introduction

menu area, the operation will be canceled.

The common functions are showed at the top right corner of the screen as shortcut key. Move the

mouse to the function you wanted.

The menu functions are as follows:

Sample Mode: Choose the sample mode among Venous Blood Mode, Capillary Blood Mode and

Prediluted Mode.

Diluent: Inject the diluent quantificationally by sample needle. This menu uses only in Prediluted

Mode.

Information: Enter the information window of the next sample, input and modify the

information.

Histogram: Enter the histogram adjustment window of the current sample. You can manually

adjust the histograms of the current sample.

Print: Output the data of the current sample to the appointed device.

Function: Enter the function menu.

Mute: Stop the alert.

Help: Access the system help window.

About: Display information of the instrument such as software and copyright etc.

Shutdown: Startup the shutdown program to rinse the measurement unit automatically. Shutoff

the power supply when the screen displays “Please switch off”.

Function Menu

Review: Enter the review window. You can review, inquire or modify the measurement data of the

former sample.

Calibration: Enter the calibration window to calibrate the instrument.

Quality Control: Enter the quality control window to carry out quality control operation.
Setting: Enter the setting menu.

Service: Enter the service menu.

Calibration Menu

Manual Calibration: Enter the manual calibration window to calibrate the instrument manually.

10
 Instrument Introduction

Auto Calibration: Enter the auto calibration window to adjust the instrument automatically.

QC Menu

QC Edit: Enter the QC edit window to edit the QC parameters.

QC Run: Enter the QC run window to carry out the selected QC files.

QC Graphs: Enter the QC graphs window to browse the selected QC graphs.

QC Table: Enter the QC table window to browse the selected QC tables.

Setting Menu

Time: Enter the time setting window to set the time of the instrument.

Printout: Enter the printout window to set the functions such as print or report.

Config: Enter the config window to set the functions such as print, sleep, clean, and display etc.

Limits: Enter the limits window to modify the limits.

Department: Enter the department window to set the department and abbreviation.

Operator: Enter the operator window to set the department, abbreviation, department and

operator.

Service Menu

Flush: To get rid of the aperture clog.

Enhanced Flush: To get rid of hard aperture clog.

Cleaning: Run the normal rinse process to rinse the measurement unit.

Enhanced Cleaning: Rinse the measurement unit with RINSE.

Drain Chambers: Empty the liquid of the counter pool.

Park: Run the Park Program to rinse and empty the tubing.

Prime Diluent: Inject the Diluent to the appointed tubing.

Prime Lyse: Inject the Lyse to the appointed tubing.

Prime Rinse: Inject the Rinse to the appointed tubing.

Maintenance: Run the maintenance program to maintain the instrument.

Mechainc: Enter the Mechainc window to verify the functions of the instrument.

11
 Instrument Introduction

Special: Enter the Special window to maintain the instrument.

1.6 DETECTION PRINCIPL

1.6.1 Detection Principles of WBC, RBC, PLT

The count principle of the instrument is based on the measurement of changes in electrical

resistance produced by a particle passing through an aperture sensor.

a) The sample blood is diluted in a conductive liquid. As blood cells are non-conductive, the

diluent is a good conductor. There are big differences between them.

b) When the diluent passes through the aperture sensor, electrodes are submerged in the liquid on

each side of the aperture to create continuous current.

c) When cells pass through the aperture, the resistance between the electrodes increases as the

cell volume increasing, as is shown in figure 1-4.According to the Ohm Formulary: U=RI

(U=Voltage I=Current R=Resistance).If I is a constant, U increases as the cell volume

increasing.

d) Passing through the magnification circuit, the voltage signal will be magnified and the noise

will be filtered, then you will gain the analytical results, as shown in figure 1-4.

e) One count pool and the detection circuit count the WBC. Another count pool and the detection

circuit count the RBC and PLT. The microprocessor of the instrument calculates and analyzes

the cells (WBC, RBC, PLT), and then gives out the histograms.

f) PLT count adopts advanced liquid, electronics and software system. It settles the repetitive

count of the cells on the side of the aperture count area.

12
 Instrument Introduction

Figure 1-4

1.6.2 Principles of HGB Measurement

Adding lyse in the blood, the red blood cell will rapidly be broken down and release hemoglobin.

Hemoglobin and lyse form a new mixture, which can absorb the wavelength of 540nm.Measure

the absorbency. Through the comparison of the absorbency between the pure diluent and the

sample，the concentration of the sample hemoglobin is calculated.

1.6.3 Volume Distribution of Blood Cell

When different types of cells pass through the aperture sensor, there will be different electrical

pulse height. Because of the evident difference of the cells sizes, the instrument is able to

differentiate the white blood cell, red blood cell and platelet by its pre-set program.

The volume distributions are as follows:

WBC 120～1000fL

RBC 82～98fL

PLT 2～35fL

The leucocytes disposed by lyse can be divided into three types, according to their volumes:

lymphocyte (LYM), mid-sized cell (MID), Granulocyte (GRAN).

LYM 35～90 fL

MID 90～160 fL

13
 Instrument Introduction

GRAN 160～450 fL

1.6.4 Results and Calculation of Parameter Measurement

The parameters of the sample blood test can be described in three ways:

a) Measured directly, such as WBC, RBC, PLT, HGB.

b) Derived from histograms, such as LYM%, MID%, GRAN%, MCV, RDW-SD,

RDW-CV, MPV, PDW, P-LCR.

c) Calculated, such as LYM#, MID#, GRAN#, HCT, MCH, MCHC, PCT.

The derivation of the formularies as follows:

 MCV derives from histograms and the instrument collecting and classifying the

erythrocytes according to its volume. It is determined by measuring the average volume

of individual erythrocytes, and the unit is fL.

 RDW represents the volume distribution of the erythrocyte populations, derived from

the RBC histogram. It can be expressed by the coefficient variation of the erythrocyte

volume as RDW-CV, and the unit is %.It can also be expressed in standard deviation of

the erythrocyte volume as RDW-SD, the unit is fL.

 MPV is the average volume of individual platelets, derived from the PLT histogram. It

represents the mean volume of the PLT populations and can be expressed in fL.

 PDW derives from the PLT histogram. It represents the geometry standard deviation (10

GSD) of the volume of the PLT populations.

 P-LCR derives from the PLT histogram. It represents the ratio of the larger PLT and the

unit is %.

 HCT(%)= RBC×MCV/10

 MCH(pg)= 10×HGB/RBC

 MCHC(g/L)= 100×HGB/HCT

 PCT(%)= PLT×MPV/10

 LYM% = 100 x AL /（AL+AM+AG）

 MID% = 100 x AM /（AL+AM+AG）

 GRAN% = 100 x AG/ （AL+AM+AG）

 LYM# = LYM% x WBC/100

14
 Instrument Introduction

 MID# = MID% x WBC/100

 GRAN# = GRN x WBC/100

AL: Number of cells in LYM area;

AM: Number of cells between lymphocyte and granulocyte area;

AG: Number of cells in GRAN area.

15
 Hardware

Chapter 2 HARDWARE

The instrument has the following hardware

CPU Board, CCU Board, cable adapter board, liquid tubing optical conpler optocoupler adapter

board, motor optocoupler adapter board, DOM card, LCD adapter board, transformer, recorder

Board, switching power supply and inverter.

2.1 CPU BOARD

2.1.1 Function

With CPU as the core, the computer system also includes some peripheral equipments, such as

DRAM, SRAM, besides there are some interfaces, such as USB interface, COM2 interface,

COM1 interface, LPT printer interface, power supply interface, mouse/keyboard interface, display

screen TFT cable interface, peripheral display interface, DOM interface. As the chart 2-1.

The basic components of the computer system are CPU, DRAM, SRAM. They also supplied the

basic running environment for software.

16
 Hardware

2.1.2 Interface

1 1、 COM1interface

2、 COM2 interface

3、 USB interface
2
4、 Mouse interface

3 5、 Keyboard interface

6、 Display TFT cable

interface

7、 VGA ： external display

interface

4 8、 DOM card interface

5
9、 LPT printer interface

6
7 8 9
Chart 2-1

2.2 CCU BOARD

2.2.1 Function

The CCU board consists of six modules:

Digital Circuit Analog/Digital converted Analog Circuit

Drive Circuit Circuit

Power Circuit HGB Measurement Circuit

2.2.2 Front Interface

17
 Hardware
1

3 4 5 7 6
Chart 2-2

18
 Hardware
11
10
8

Chart 2-3

1. Cpu Interface 2. Power Supply Interface

3 .Step Motor C Sensor And Optocoupler Interface 4. Valve Interface

5. Liquid sensor interface 6. HGB Cable Interface

7. Temperature Sensor Interface. 8. 110v Ignition Voltage Input Interface

9. Probe Electrode Cable Interface 10. Indicator Led Interface

11 Recorder 20pin Flat Cable Interface

2.3 TFT Cabl e Convert in g Boar d

2.3.1 Function

The function of TFT Cable Converting Board is to connect all blocks and CCU board, and to

adjust the sensitivity of liquid sensor through adjusting the trimmer on TFT Cable Converting

Board.

2.3.2 Interface

19
 Hardware

Liquid sensor jack Valve jack Motor and optocoupler jack

Adjustable potentiometer Chart 2-4

2.4. LCD adopt er

2.4.1. Function

The main function of LCD is to connect screen and CPU with cable and TFT through FPC soft

cable. It is used to transmit signal from CPU to display screen. Display as following: chart 2-5.

FPC soft cable socket can contact with screen directly

TFT cable jack (to contact with MTB board ) directly.

Chart 2-5

20
 Hardware

2.5 RECORDER BOARD

2.5.1 Function

The main functions of the recorder board are to receive and deal with the printing data, and then

transmit the data to thermal printer. At the same time, the recorder board drives the motor to print

the received data. It is as the chart

Power supply
interface

Record cable interface

Chart 2-6

2.6 DOM card

2.6.1 Function

DOM card is used for deposition. It should make sure the DOM card is in correct way before

insert it. As the following chart:

Drawing tablets

DOM jack
Drawing tablets

Chart 2-7

21
 Tubing

Chapter 3 TUBING

3.1 TUBING GRAPH

Chart 3-1

22
 Tubing

3.2 STRUCTURE OF TUBING SYSTEM

3.2.1 Liquid road structure

1、 Vacuum pump machine.

8 2、 Lyse sensor.
6
3、 Vacuum pump block.
1
4、 Dilution sensor.
7 2
5、 Waste liquid sensor.
4
6、 Dilution block

7、 Sample machine.
3
8、 Glass bulb

9、 Cleaner sensor.

9 5 Chart 3-2

3.2.2 Tubing Sensor Unit

The instrument includes four tubing sensor: diluent tubing sensor, rinse tubing sensor, lyse tubing

sensor, waste liquid sensor and reagent temperature sensor.

The tubing sensor, rinse tubing sensor, lyse tubing sensor are sued to check if the reagent enters

into the tubing system. If there is no reagent in the tube. The instrument will give alarm of “no

diluent” or “no cleaner” or “no lyse”.

Waste liquid sensor is used to test if the waste liquid is full. If yes, there instrument will give alarm

of “waste liquid full.”

reagent temperature sensor is used to check the reagent temperature in the tube. If the temperature

is too low the instrument will give alarm of “temperature too low”.

23
 Tubing

3.2.3 HGB Unit

This part is used to measure the hemoglobin concentration.

3.2.4 Dilute Unit

This part consists of sample sucker structure and dilution structure. Sample sucker structure is

used to aspirate sample, add it into the probe structure and clean the sample needle. Dilution

structure is used to provide power to suck sample.

3.2.5 Vacuum Unit

This part is made up of vacuum pump and vacuum decelerating structure.

The vacuum decelerating structure is working under the system software and is used to provide

power in the condition of electrifying. Vacuum pump is driven by vacuum decelerating structure

and produce negative pressure. So that to provide working power to all this tube system such as

waste emissions.

3.2.6 Auxiliary Unit

This part mainly refers to the tubing hose, connecting tube and electromagnetically operated

valve.

24
 System Structure

Chapter 4 SYSTEM STRUCTURE

4.1 DISASSEMBLE/REPLACE PARTS AND COMPONENTS

4.1.1 Disassemble/Replace the Sample Machine

Block

slider

Chart 4-1

25
 System Structure

It is necessary to replace the sample needle wipe block, seal washer and synchronous driving belt

of the sample machine periodically. The replacing cycle is one year.

4.1.2 Disassemble/Replace the Diluent Machine

4.1.3 Disassemble/Replace the Vacuum Block

26
 System Structure

4.1.4 Disassemble/Replace the Vacuum Pump Block

It is necessary to replace the seal washer of the vacuum pump periodically. The replacing cycle is

one year.

27
 System Structure

4.1.5 Disassemble/Replace the Piston Block

It is necessary to replace the seal washer of the piston block periodically. The replacing cycle is

one year.

28
 System Structure

4.1.6 Disassemble/Replace Chamber

Chart 4-6

29
 System Structure

Chart 4-7

30
 Adjustment

Chapter 5 ADJUSTMENT

This part introduces how to adjust the gain of the passage.

5.1 GENERAL

5.1.1 Replace the chamber

2800 probe components: RBC, WBC gain, HGB gain.

5.1.2 Replace the Aperture

Aperture: WBC gain, RBC gain

5.1.3 Replace CCU board

Need to adjust the gain of the WBC, RBC/PLT, HGB.

5.2 ADJUST PROGRAM

5.2.1 Adjust calibration parameter.

In main operation window of the instrument, click the right key of the mouse, pops up the menu.

Move the cursor to “Calibration/Manual Calibration”. Adjust the calibration factors to 100%.

Click the right key of the mouse, and pops up the menu, then move the cursor to

“Service/Maintenance”, enter the password “6666”, and click “F12”key.

Click the right key of the mouse, pops up the menu, then move the cursor to

“Calibration/Factory Manual Calibration”. Adjust the calibration factors to 100%.

Use the calibration of Procan recommended. In main operation window, measure the calibration.

According to the measured histograms to adjust the gain of the passage. Adjust the gain of passage

WBC, RBC according to the Multi-Parameter Assayed Hematology Control Expected Results

Sheet.

In main operation window of the instrument, click the right key of the mouse, pops up the menu.

then move the cursor to “Service/Test”, enter the password “6800”,click “HGB Background/T”

key to adjust the potentiometer VR5,setting the voltage as 4.31±0.02V.

31
 Appendix 2 Debug record form

5.3 ADJUST PLACE

12

1. Adjustment of WBC Gain 2. Adjustment of RBC Gain

3. Adjustment of HGB Gain

32
 Maintenance

Chapter 6 MAINTENANCE

As other precision instrument, only careful daily service and periodic maintenance can the

instrument have a good working status, and can we get the reliable measurement results and have

less malfunctions. This chapter introduces some preventive methods for service and maintenance.

If you want to know more relative information, please contact the customer service department of

Procan.

According to the requirements for maintenance of the instrument during the using procedures, we

divide the preventive service and maintenance into the following types: daily, weekly, monthly,

yearly and maintenance according to actual need.

Warning:

If the hospital or agency don’t perform a satisfying plan to maintain the instrument, there

will be abnormal malfunction.

6.1 ROUTINE MAINTENANCE

6.1.1 Clean the Appearance of the Instrument

Wipe the appearance of the instrument with neutral detergent or distilled water.

Caution:

Avoid using corrosive acids, alkali, and volatile organic solvent such as: acetone, aether,

chloroforms to wipe the appearance of instrument. Only can use the neutral detergent.

Caution:

Avoid wiping the inner of the instrument.

33
 Maintenance

6.1.2 Replace Fuse

The fuse is installed in the fuse-box on the side of power switch. Open the box to replace the fuse

expediently.

Appointed specification fuse: AC 250V 2A

Warning:

Only can use the appointed specification fuse.

6.2 DAILY MAINTENANCE

It can be divided into two types: run and shutdown.

 Run

The instrument has installed daily maintenance procedure. On running, it can

execute auto cleaning procedure according to the quantity of the sample to keep the

instrument in good working status. Set the auto cleaning procedure according to the

section 6.3. The general setup principles are:

Working time > 8 hours, auto-cleaning time=8 hours;

4 hours<Working time<8 hours, auto-cleaning time=4 hours;

Working time< 4 hours, auto-cleaning time=2 hours

The auto-cleaning time will decrease 1 hour year by year.

 Shutdown

The instrument should be operated by auto maintenance before shut off the instrument.

You only need to clean the workbench and wipe the appearance of instrument when the

power-off.

6.3 WEEKLY MAINTENANCE

This instrument has installed weekly maintenance procedure. Act as follows:

a) Prepare E-Z cleanser and probe cleanser.

b) In the main operation window, press the right key of the mouse, pops up the menu.

34
 Maintenance

In the menu area, select “Service/Maintenance”. Operate as the screen shows.

c) Turn off the power supply.

d) Wipe the appearance of instrument as section 6.1.1.

6.4 MONTHLY MAINTENANCE

It is necessary to clean the dust of the instrument monthly.

Operate as follows:

a) Turn off the power supply, and pull out the power line.

b) Hold up the air filter cover with screwdriver.

Take off the cover and the net. Clean them with neutral detergent brush and put them in the

shade.

c) Airing naturally .Brush away the dust clinging on the air filter grid at the rear panel of

instrument.

d) Put the air filter grid on the air filter net carefully. Planish them.

Notice:
Only can use the neutral detergent cleaning the air filter. Avoid airing it with heat.

6.5 YEARLY MAINTENANCE

It’s necessary to perform preventive maintenance once every year. Because of the

highly-requirements for yearly maintenance, the maintenance should be performed by the

authorized engineer of Procan. Please contact the customer service department of Procan before

yearly maintenance.

6.6 THE MAINTENANCE BEFORE TRANSPORT OR FOR

INSTRUMENT THAT WILL NOT BE USED FOR A LONG TIME.

If the instrument is not going to be used in 2 weeks or longer, or need to pack and transport,

performing the following procedures:

a) In the main operation window, press the right key of the mouse, choose “Service/Park”

35
 Maintenance

in the menu.

b) Operate as screen shows.

c) Rotate the bottle covers of rest reagents and store them as the reagent operation

introduction. User should perform efficient action to prevent the material from

deteriorating, mis-eating and misusing.

d) Plug the stopples which were pulled out at the first installation into the corresponding

tube connector.

e) Pull out diluent connection tube, rinse connection tube, lyse connection tube and waste

connection tube and clean them with distilled water. Dry them in shady place, then

packing them into plastics.

f) Pull out the power wire, packing it in plastics after cleaning with neutral detergent.

g) Put the instrument and parts packed in plastics into packing cases.

36
 Troubleshooting

Chapter 7 TROUBLESHOOTING

7.1 PROGRAM TEST

Test instrument through the following programme:

Make sure the Start instruments,

ambient Start up abnormal
observation equipment
temperature
self-test is normal, all Mainten
humidity NG
moving parts is running ance
atmospheric Moving parts run
smoothly, and liquid road
pressure are in abnormal
system without leakage and
other abnormalities,
apparatus with alarm or not Liquid road
system leakage
Make sure if the
reagent is enough, OK
srcinal reagent
and not ex ired
Blank data does not
Blank Test
meet the technical
Observe the test
requirements
data and data gaps
Make sure the NG Maintenan
is in compliance
voltage is correct
with technical The amount of data
to our instrument
requirements or does not meet the
not technical requirement
--- Hi h
OK
Make sure the
reagent is
Sample test
connected correct NG
The test result is Sample tests do
and reliable Maintenance
steady or not (CV not meet
value is accord with technical

technical
Make sure if any requirement
part is connected
correctly and OK
reliable.
END

Chart 7-1

37
 Troubleshooting

7.2 CHECKING ITEMS BEFORE MAINTENANCE

Items required for checking before maintenance:

Ambient Temperature: 15～35℃

Temperature of the reagent: 15～30℃

Humidity: 10～90%

Atmospheric Pressure: 86.0kPa～106.0kPa

If the temperature is below 15 ℃,the activity of the reagent will reduce. It will weaken the capacity

of dissolving the blood. In a certain time, it can not break down the red blood cell membranes into

2fl cellular debris. At lower temperature conditions, the red blood cell debris will gather together.

It will affect the measurement of leukocyte, resulting in the count value of WBC increasing .It will

also affect the measurement of HGB, resulting in the count value of HGB increasing.

Checking for Sample Blood

Sample Collection: Observe whether the operator uses correct method to collect the blood

(Operating as PE-6800 FULLY AUTO HEMATOLOGY ANALYZER OPERATION MANUAL

/Chapter 3/Section 3.5 Preparation For Sample Collection), and whether the sample is stored in

the container filling with EDTA.K2O.H2O anticoagulant.

Sample Storage: After the sample blood is well-mixed, test on the instrument promptly. As time

goes by, the volume and density of the cells will change. The environment and the patient

determine the varying rate of the volume and density. If the sample is placed a long time at room

temperature, the volume of RBC will increase, and the MCV, RDW, PDW will be affected, and the

PLT will easily gather together.

Affection of WBC Classification: To get the exact measuring results, the venous blood sample

measurement must be completed within 4 hours. If the sample blood is placed a long time at room

temperature, the total number of WBC may change little, but the classification is inaccurate.
7.3 BACKGROUND CHECK

Background Check: This item can ensure the measurement and the calculated data won’t be

affected by reagent empty. If the background value exceeds the permissive area, the measured and

calculated data will be inaccurate.

The permissive range of the background test results is

38
 Troubleshooting

Parameter Background Range Unit

9
WBC
9
≤0.2×10 /L 10 /L
12
RBC
12
≤0.02×10 /L 10 /L

HGB ≤1g/L g/L

HCT ≤0.5％ %
9
PLT
9
≤10×10 /L 10 /L

Background Test Method: When power-on, suggested to run background test, you can also

execute background test as you need.

The operation steps are as follows:

a) In the blood cell analyzer window, press “Info” button, and pops up the information

edit window. Then click the item textbox, change the ID as 999999999.then press

“OK”, return to blood cell analyzer window.

b) Background count in venous or capillary mode, press the “START” key below the

sample needle directly for background count. In prediluted mode, the sample needle

aspirates some non-contaminated diluent from the chamber for background count.

The diluent pick-up method as follows(Analyzing the sample in prediluted mode, it

is also necessary to dilute sample with the diluent which is prior picked-up in this

method):

In main operation area, press the right key of the mouse, pops up the menu. In menu operation

area, select “Sample Mode/ Prediluted” to set the sample mode as prediluted mode.

In main operation area, press the right key of the mouse, pops up the menu. In the menu operation

area, select “Diluent”. Place a clean empty chamber or tube under the sample needle, press

“START” key to dispense the diluent into the chamber. Press the “START” key once, the diluent

is dispensed into the chamber or tube once (The quantity can be used for background count in the

second prediluted mode).

Press “Exit” button to return to the main operation area.

Affected Factors of Background:

1、 The reagent is dirty.

2、 The chamber is dirty.

39
 Troubleshooting

3、 Electronic interference affects measurement and the calculated circuit, for example:

Interference created by voltage fluctuation, peripheral equipment such as centrifugal

machine, CT machine, magnetic resonance device, etc.

4、 Tubing Contamination

40
 Lead label

Chapt er 8 Ap pendi x 1 Lea d label

No. Stick place Label name note

Sample structure monitor in the

1 Motor A
2 Motor B
MA
kickstand place
Sample structure monitor in
MB
horizontal axis fixed plate

3 Motor C MC Dilution monitor

4 Motor D MD Vacuum decelerating monitor

Sample structure switch in the

5 Optocoupler A OSA
kickstand place
Sample structure switch in
6 Optocoupler B OSB
horizontal axis fixed plate

7 Optocoupler C OSC Dilution switch

8 Optocoupler D OSD Vacuum decelerating switch

9 Dilute sensor DLS

10 Lyse sensor LYS

11 Cleaner sensor CLS

Waste liquid
12 WST
sensor

13 switch STA

14 MTB board MTB

The lead is according to the No.

15 valve V1.V2…V16
of valve

41
 Debug record form

Chapter 9 Appendix 2 De bug record for m

Name : Host debugging technics No: F-6807-30-02301 Edition：
Serial No. of product： Temperature： Humidity：
Item Requirement Result Note
 OK
appearance According to the requirement of standard products
 Not ok.
Refer to PE-6807 the requirement of “host production  OK
fitting requirement”  Not ok.
Should display full screen and every label should  OK
Display screen
clear and no color cast  Not ok.
Check the connection between jack and mouse is  OK
Mouse interface
connected well and can work in good way.  Not ok.
Check the connection between jack and keyboard is  OK
Keyboard interface
connected well and can work in good way.  Not ok.
It will light when standby and shine when suck  OK
Indicator light
sample.  Not ok.
 OK
Reagent alarm With words and sound alarm
 Not ok.
 OK
Backboard fan With right direction and without noice cacophony
 Not ok.

Date and time Local date and time  OK

 Not ok.
With correct place and there is sound when connected  OK
valve
with electricity.  Not ok.
 OK
Data circuit It will display “ok” when we check it.
 Not ok.
HGB black voltage Adjust to 4.31V V
Chamber have sound of “ Chichi”. The indicator light  OK
Burning function
will be on.  Not ok.
Motor and Smooth operation, no abnormal sound, non-blocking
 OK
associated moving phenomenon
 Not ok.
parts And the status of the test results should be "OK"
Sampling needle tip
position Adjust the position with the sampling needle position  OK
perpendicular to the tool  Not ok.
direction of srcin
Negative pressure of
79.2±1 KPa KPa
vacuum chamber
Vacuum chamber
≤0.2 KPa /2Min KPa /2Mins
leak rate
5.0V voltage test 5.00±0.5V V

42
 Debug record form

Reagent temperature Temperature of thermometer should be ±5 ℃ the

℃
test same as temperature inside of chamber
Click up
Aperture 9～20V V
voltage
Aperture voltage test
Click down
Aperture Less than3V V
voltage
Draining the amount
of pre-diluted mode 8.0±0.4ml ml
Test the voltage of liquid sensor is 3.0V ～5.0V with
V
Test the voltage of reagent
liquid sensor Test the voltage of liquid sensor is ≤1.5V without
V
reagent
 OK
Air Mixer There is air bubble come out from bottom of cup.
 Not ok.
The ability of
chamber shield  OK
Resistance should be more than 20M Ω
(cover) and the  Not ok.
substrate insulation
Lymphocyte peak fL
Channel gain
RBC peak fL
9
WBC ≤0.2×10 /L 0×109/L The
12 12
RBC ≤0.02×10 /L 0×10 /L  OK
average of
Take count in blank HGB ≤1g/L 0g/L  Not ok
blank test
HCT ≤0.5％ 0％
9 9
result
PLT ≤10×10 /L 0×10 /L
The
average
Oversee the time of □ OK value of 5
12S+1S
blank counting □ Not ok times
counting
time
WBC V Every time
Set the Reference
of blank
voltage value of
RBC V test is
pinAperture ok.

The liquid should flow cling to the needle uniformly

Add liquid cleaning □ OK
and there is no departure of needle and no share
kits □ Not ok.
phenomenon

Channel data volume WBC ≤200KB  OK Any one

RBC ≤100KB  Not ok. blank test

43
 Debug record form

PLT ≤300KB result

WBC ≤0.5％
Carrying RBC ≤0.5%  OK
contamination test HGB ≤0.5%  Not ok.
PLT ≤1.0%
Venous WBC
and RBC
Periphera HGB
l blood MCV  OK
calibratio 
n Not ok.
Instrument PLT
paramete
calibration
r
WBC
Pre
RBC
dilute  OK
HGB
calibration  Not ok.
MCV
parameter
PLT
WBC accuracy test ≤3.0%
RBC accuracy test ≤3.0%  OK
Accuracy test（CV） HGB accuracy test ≤3.0%  Not
MCV accuracy test ≤1.0% ok.
PLT accuracy test ≤7.0%
Venous blood Predilution
model model

WBC accuracy ±
error range 6%
RBC accuracy ±
error range 5%
Test accuracy  OK
HGB accuracy ±
 Not
error range 4%
ok.
MCV accuracy ±
error range 5%
PLT accuracy ±
error range 14%
Printing characters, graphics should be correct, clear. The
functioning of institutions should be smooth. There is no abnormal □ OK
Record and alarm
sound, no paper block but with alarm function phenomenon, there □ Not ok.

are alarm.
Pinter and net Printing characters, graphics should be correct, clear.Meanwhile □OK
function the PC can accept complete and correct data and graphic. □Not ok.

WAperture
□OK □ Not ok.
estimation.

44
 Troubleshooting

Chapter 10 Appendix 3 TROUBLESHOOTING

Error Message Possible Reason Troubleshooting

Fuse burn-out Fluctuating input AC voltage Use stabilized voltage power supply

Power supply board is short circuit Replace new power supply board

There are bubbles in chamber Exclude the bubbles

The reagent is inadequate Re-prime the reagent

Bubbles The setup of count time is too long Reset the count time

The aperture is broken Renew the aperture

The count passage leaks Replace the leakage count tubing

The back cover and body of chamber lacks Replace the the back cover of sample
cover

Abnormal The reagent is contaminated Renew the reagent

background test Electronic interference Connect the ground wire

Tubing contamination Clean the tubing

There are large-cells or debris in the Remove the debris from the sample
sample

The setup of count time is too short Reset the count time
Clogs
Aperture clogs Clean the aperture

Inadequate diluent Check for diluent and piston block

MTB is broken Replace MTB board

Unclear display Incorrect connection of the signal line Re-connect the signal line

Liquid crystal display screen is aging Replace the display screen

Abnormal HGB The WBC chamber is dirty , Dip in the chamber ,or adjust the gain
of HGB
background The battery is not get enough light Replace battery or WBC probe
voltage components
LBD Is Not Light Enough Replace the LBD or WBC probe
components
Some keys of the The CPU board or the keyboard is broken Replace the CPU board or the
keyboard is keyboard
irresponsive

45
 Troubleshooting

Liquid leaks Abnormal vacuum chamber pressure Replace vacuum chamber

The waste tubing is obstructed Dredge the waste tubing

Abnormal The sample is placed a too long time Measure the sample within 30
mid-sized cell minutes
percentage The anticoagulant is superfluous Reduce the quantity of anticoagulant.

Reagent is used up Renew the diluent

No reagent, Fluid hose seal in bad way Check the connection and get rid of

liquid lack
Rinse(diluent/lyse)
The piston block leaks Replace the piston block or seal ring
empty alarm The rinse(diluent/lyse) sensor is broken ,or Replace the rinse(diluent/lyse) sensor
the connector is broken off

The chamber is dirty Clean the chamber

The count time is longer than setup time Reset the count time

WBC/ RBC clogs One sensor of the MTB board is broken Replace MTB board

The reference voltage of Aperture is much Reset the reference voltage of

lower than the actual test values Aperture

There is no result There are problems on CCU board Replace or repair CCU board

of RBC, WBC The electrode cable is broken Replace or repair probe components

measurement

The instrument is effected by high Get rid of the electromagnetic

electromagnetic machine. Such as the machine.
instrument is not connected well with

The result of RBC, ground

The liquid road is dirty Clean the liquid road and
WBC is high operate maintenance programme.

The sample is stared too long time Get new samples.

46