KP stock 500 mg/ml 5001.9 Glycine stock 0.01 0.

25
CSLE 20 mg/ml 1% Glycine g/ml 25
200 10
mg ml
Vary Conc CSLE

Kolliphor CSLE
mg ml mg ml Adjust Tbutyl to V (ml) conc CSLE (mg/ml)
1 200 0.4 10 0.5 3.1 4 2.50
2 200 0.4 12 0.6 3 4 3.00
3 200 0.4 14 0.7 2.9 4 3.50
4
5 200 0.4 16 0.8 2.8 4 4.00

Kolliphor CSLE
mg ml mg ml Adjust Tbutyl to V (ml)
TEM Blank 200 0.4 0 0 3.6 4
Sam1 200 0.4 15 0.75 2.85 4
Drug release Sam2 200 0.4 15 0.75 2.85 4
Sam3 200 0.4 15 0.75 2.85 4
Sam4 200 0.4 15 0.75 2.85 4
Sam5 200 0.4 15 0.75 2.85 4
Sam6 200 0.4 15 0.75 2.85 4
Sam7 200 0.4 15 0.75 2.85 4
FT-IR / HPLC Blank 0 0 15 0.75 3.25 4
Sam8 200 0.4 15 0.75 2.85 4
Stability Sam9 200 0.4 15 0.75 2.85 4
Sam10 200 0.4 15 0.75 2.85 4
Sam11 200 0.4 15 0.75 2.85 4
Sam12 200 0.4 15 0.75 2.85 4
Sam13 200 0.4 15 0.75 2.85 4
Sam14 200 0.4 15 0.75 2.85 4
DSC Sam15 200 0.4 15 0.75 2.85 4
Glycine Sam 16
6.8
g 12500 12.5 g
ml 500 mg

Add water %w/v glycine

4 1
4 1
4 1
1
4 1

conc CSLE (mg/ml) Add water

0.00 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
3.75 4
Sample CLSE-KP

KP (mg) CSLE (mg) to V (ml)

200 4 3
200 6 3
200 8 3
200 10 3
800 2 3
800 8 4
800 10 4
800 12 4
800 14 4
1000 8 4
1000 10 4
1000 12 4
1000 14 4
1200 4
1200 10 4
1200 12 4
1200 14 4

KP stock 500 mg/ml 5001.4 mg

CSLE 10 mg/ml 103.6 mg หมายเหตุ เตรียมแบบไม่ใส่ glycine ด้วย

Kolliphor (mg/ml) CSLE (mg/ml)

mg ml mg ml Adjust Tbutyl to V (ml) conc CSLE (mg/ml)
200 0.4 10 1 2.6 4 2.50
300 0.6 10 1 2.4 4 2.50
400 0.8 10 1 2.2 4 2.50
500 1 10 1 2 4 2.50

Blank

Kolliphor (mg/ml)
mg ml Adjust Tbutyl to V (ml) Add water
200 0.4 3.6 4 4
300 0.6 3.4 4 4
400 0.8 3.2 4 4
500 1 3 4 4

KP stock 500 mg/ml 5001.9 Glycine stock 0.01 0.25

 CSLE 20 mg/ml 1% Glycine g/ml 25
200 10
mg ml
Vary Conc CSLE

Kolliphor CSLE
mg ml mg ml Adjust Tbutyl to V (ml) conc CSLE (mg/ml)
1 200 0.4 10 0.5 3.1 4 2.50
2 200 0.4 12 0.6 3 4 3.00
3 200 0.4 14 0.7 2.9 4 3.50
4 200 0.4 15 0.75 2.85 4 3.75
5 200 0.4 16 0.8 2.8 4 4.00

Vary glycine

Kolliphor CSLE
mg ml mg ml Adjust Tbutyl to V (ml) conc CSLE (mg/ml)
6 200 0.4 10 0.5 3.1 4 2.50
7 200 0.4 10 0.5 3.1 4 2.50
8 200 0.4 10 0.5 3.1 4 2.50
 1%glycine (mg/ml) Add conc CSLE (mg/ml)
1.33
2.00
0.03 3 2.67
0.03 3 3.33
0.03 0.67
0.04 4 2.00
0.04 4 2.50
0.04 4 3.00
0.04 4 3.50
0.04 4 2.00
0.04 4 2.50
0.04 4 3.00
0.04 4 3.50
0.04 4 #VALUE!
0.04 4 2.50
0.04 4 3.00
0.04 4 3.50

มแบบไม่ใส่ glycine ด้วย Glycine stock 1%

glycine 250 mg ในน้ำ 25 ml 250

Add water 1% glycine

4 0.04
4 0.04
4 0.04
4 0.04

g
ml

Add water %w/v glycine

4 1
4 1
4 1
4 1
4 1

Add water %w/v glycine glycine (g) mg to 5 ml

4 1 0.05 50 5
4 2 0.1 100 5
4 4 0.2 200 5
 Sample
Date KP CSLE Peak 1 (nm) Peak 2 Peak 3
11/25/2023 200-1 4 17.14 3860 0
200-2 4 17.11 4392 0
200-3 4 16.4 3858 0
200-1 6 16.97 4670 0
200-2 6 17.5 4052 0
200-3 6 18.44 4100 0
200-1 8 17.15 2104 0
200-2 8 16.65 3360 0
200-3 8 17.75 2360 0
200-1 10 19.14 1928 0
200-2 10 17.09 789.7 4482
200-3 10 19.52 1688 0
11/26/2023 200-1 2
200-2 2
200-3 2
300-1 2
300-2 2
300-3 2
400-1 2
400-2 2
400-3 2
600-1 2
600-2 2
600-3 2
800-1 2
800-2 2
800-3 2
1000-1 2
1000-2 2
1000-3 2
11/16/2023 200-1 10 18.76 2462 0
CSLE@KP 200-2 10 17.72 2387 0
200-3 10 19.88 3272 0
300-1 10 17.37 3807 0
300-2 10 15.9 4165 0
300-3 10 16.65 4262 0
400-1 10 14.48 4647 0
400-2 10 16.15 0 0
400-3 10 15.71 4039 0
500-1 10 15.53 0 0
500-2 10 16.15 3891 0
 500-3 10 16.84 2477 0
11/16/2023 200-1 10 17.09 3940 0
CSLE@KP + glycine 200-2 10 16.7 0 0
200-3 10 16.81 0 0
300-1 10 19.33 4123 0
300-2 10 19.15 3760 0
300-3 10 18.42 3950 0
400-1 10 15.24 4437 0
400-2 10 15.92 0 0
400-3 10 15.79 0 0
500-1 10 20.4 4434 0
500-2 10 17.93 4429 0
500-3 10 18.96 4357 0
200-1 10
200-2 10
200-3 10
200-1 12
200-2 12
200-3 12
200-1 14
200-2 14
200-3 14
200-1 15
200-2 15
200-3 15
Z Average PDI %EE
15.39 0.246
15.58 0.247
14.41 0.234
21.76 0.052
15.38 0.256
16.76 0.228
17.47 0.311
15.52 0.285
16.9 0.314
22.44 0.368
73.53 0.167
21.95 0.348

20.05 0.356 11/16/2023 KP Peak 1 (nm) Peak 2 Peak 3

19.61 0.331 Blank 200-1 18.73 4169 0
20.78 0.335 200-2 19.01 3663 0
16.27 0.271 200-3 17.96 3026 0
13.14 0.232 300-1 13.2 260.1 0
13.9 0.214 300-2 13.58 430.7 5047
15.21 0.126 300-3 14.54 460.4 5000
13.41 0.2 400-1 13.62 0 0
12.98 0.196 400-2 17.32 4673 0
13.22 0.191 400-3 17.26 3877 0
14.79 0.261 500-1 13.81 1575 4848
17.14 0.308 500-2 13.74 4165 0
17.16 0.169 500-3 15.47 1127 0
13.33 0.191
13.77 0.215
28.31 0.073
18.26 0.252
17.43 0.265
15.33 0.11
13.19 0.161
13.13 0.187
17.71 0.226
16.94 0.242
15.65 0.198
Z Average PDI
17.58 0.265
18.43 0.777
17.89 0.256
64.16 0.144
15.49 0.347
17.64 0.386
12.31 0.103
15.58 0.236
15.73 0.222
14.67 0.281
13.67 0.237
16.35 0.327
 HPLC %EE

Standard CBD 95% solution 1 mg/ml

Conc (mg/ml) Pipet Stock (ml) to V Conc Std (ug/ml) Pipet Stock (ml) ul
0.5 5 10 0.5 10
A 1 10 10 1 0.01 10
B 2.5 25 10 2.5 0.025 25
C 5 50 10 5 0.05 50
D 7.5 75 10 7.5 0.075 75
E 10 100 10 10 0.1 100

Sample CLSE-KP

KP (mg) CSLE (mg) to V (ml) conc CSLE (mg/ml) Pipet (ml) ul

200 4 3 1.33 0
200 6 3 2.00 0
1 200 8 3 2.67 0.01 10
2 200 10 3 3.33 0.01 10
800 2 3 0.67 0.01 10
3 800 8 4 2.00 0.01 10
4 800 10 4 2.50 0.01 10
5 800 12 4 3.00 0.01 10
6 800 14 4 3.50 0.01 10
7 1000 8 4 2.00 0.01 10
8 1000 10 4 2.50 0.01 10
9 1000 12 4 3.00 0.01 10
10 1000 14 4 3.50 0.01 10
11 1200 8 4 2.00 0.01 10
12 1200 10 4 2.50 0.01 10
13 1200 12 4 3.00 0.01 10
14 1200 14 4 3.50 0.01 10
 อุปกรณ์
1. Volumetric flask 10 ml 22 ขวด
to V 2. Micropipet 10 ul Peak Area
10 3. HPLC vial 22 ขวด Conc (ug/ml) 1 2
10 A 1 38437 39420
10 สารเคมี B 2.5 101936 104509
10 1. Acetonitrile C 5 210752 207190
10 2. MilliQ water D 7.5 307061 308621
10 3. Methanol E 10 409648 409603

Peak Area
to V (ACN) Dilute (mg/ml) ug/ml 1 2 3 Average dilution factor
5 0.00 - - - - 0.002
10 0.00 - - - - 0.002
5 0.005333 5.33 55709 55561 55635 0.002
5 0.006667 6.67 79651 80186 79918.5 0.002
10 0.000667 0.67 - - - - -
5 0.004000 4.00 47003 47547 47275 0.002
5 0.005000 5.00 52927 61748 57337.5 0.002
5 0.006000 6.00 64224 64068 64146 0.002
5 0.007000 7.00 80776 80586 80681 0.002
5 0.004000 4.00 43057 43361 43209 0.002
5 0.005000 5.00 44870 45482 45176 0.002
5 0.006000 6.00 55514 56408 55961 0.002
5 0.007000 7.00 46864 47811 47337.5 0.002
5 0.004000 4.00 43319 44182 43750.5 0.002
5 0.005000 5.00 52625 56535 54580 0.002
5 0.006000 6.00 69459 71119 70289 0.002
5 0.007000 7.00 70754 72119 71436.5 0.002
 Chart Title
450000
400000
3 Average f(x) = 41069.1735459662 x + 217.76422764227
350000
R² = 0.999802404233225
39825 39227.33333 300000
103222.5 250000
208971 200000
307841 150000
100000
409625.5
50000
0
0 2 4 6 8 10 12

Conc CBD
Conc*Dilu Conc CSLE@KP ug/Conc CSLE@KP mg *Dilu mg %EE CLSE(mg) * 32.43
0.0000 - - - -
0.0000 - - - -
2.6667 1.3494 0.0013 2.0241 78.02 2.5944
3.3333 1.9407 0.0019 2.9110 89.76 3.243 1.60
- - - 0.6486
2.0000 1.1458 0.0011 2.2916 88.33 2.5944
2.5000 1.3908 0.0014 2.7816 85.77 3.243
3.0000 1.5566 0.0016 3.1132 80.00 3.8916
3.5000 1.9592 0.0020 3.9184 86.31 4.5402
2.0000 1.0468 0.0010 2.0936 80.70 2.5944
2.5000 1.0947 0.0011 2.1894 67.51 3.243
3.0000 1.3573 0.0014 2.7146 69.76 3.8916
3.5000 1.1473 0.0011 2.2947 50.54 4.5402
2.0000 1.0600 0.0011 2.1200 81.71 2.5944
2.5000 1.3237 0.0013 2.6474 81.63 3.243
3.0000 1.7062 0.0017 3.4124 87.69 3.8916
3.5000 1.7341 0.0017 3.4682 76.39 4.5402

Dilution
(Conc * Total Dilute V) * Total V / Pipet
 HPLC %EE

Standard CBD 95% solution 1 mg/ml

Conc (mg/ml) Pipet Stock (ml) to V Conc Std (ug/ml) Pipet Stock (ml) ul to V
0.5 5 10 0.5 10 10
A 1 10 10 1 0.01 10 10
B 2.5 25 10 2.5 0.025 25 10
C 5 50 10 5 0.05 50 10
D 7.5 75 10 7.5 0.075 75 10
E 10 100 10 10 0.1 100 10

Stock (mg/ml) 5 25 mg 5 ml
F 15 0.03 30 10
G 20 0.04 40 10
H 25 0.05 50 10

Peak Area
Conc (ug/ml) 1 2 3 Average Chart Tit
A 1 1200000
B 2.5 f(x) = 48030.9317073171 x
1000000 R² = 0.998067017617731
C 5
D 7.5 308658 307301 307979.5 800000
E 10 409648 409603 409625.5
F 15 639331 632302 632986 634873 600000
G 20 910627 914944 912785.5
400000
H 25 1135586 1133216 1133204 1134002
200000

0
0 5 10 15

Sample CLSE-KP CBD content 32.43

KP (mg) CSLE (mg) to V (ml) conc CSLE (mg/ml) Pipet (ml) ul to V (ACN)
1 200 10 4 2.50 0.1 100 5
2 300 10 4 2.50 0.1 100 5
3 400 10 4 2.50 0.1 100 5
4 500 10 4 2.50 0.1 100 5
5 200 10 4 2.50 0.1 100 5
6 300 10 4 2.50 0.1 100 5
7 400 10 4 2.50 0.1 100 5
8 500 10 4 2.50 0.1 100 5
9 200 10 4 0.1 100 5
10 300 10 4 0.1 100 5
11 400 10 4 0.1 100 5
12 500 10 4 0.1 100 5
 อุปกรณ์
1. Volumetric flask 10 ml 22 ขวด
2. Micropipet 10 ul
3. HPLC vial 22 ขวด

สารเคมี
1. Acetonitrile
2. MilliQ water
3. Methanol

Chart Title

f(x) = 48030.9317073171 x − 64626.3414634147

R² = 0.998067017617731

0 5 10 15 20 25 30

Peak Area
Dilute (mg/ml) ug/ml 1 2 3 Average Conc CBD ug/ml Conc CBD mg/ml
0.050 50.00 537635 542655 548767 543019.00 12.65 0.0127
0.050 50.00 575667 587055 591358 584693.33 13.52 0.0135
0.050 50.00 407380 409495 408937 408604.00 9.85 0.0099
0.050 50.00 515324 525194 531453 523990.33 12.25 0.0123
0.050 50.00 481408 486318 490954 486226.67 11.47 0.0115
0.050 50.00 515172 517646 521345 518054.33 12.13 0.0121
0.050 50.00 495380 504211 514511 504700.67 11.85 0.0119
0.050 50.00 479921 478093 477905 478639.67 11.31 0.0113
0.000 0.00
0.000 0.00
0.000 0.00
0.000 0.00
CBD content *Dilu mg %EE Drug content
3.2430 2.5302 78.02 1.2449
3.2430 2.7038 83.37 0.8916
ไม่มี glycine
3.2430 1.9705 60.76 0.4887
3.2430 2.4510 75.58 0.4870
3.2430 2.2937 70.73 1.1286
3.2430 2.4263 74.82 0.8001
มี glycine
3.2430 2.3707 73.10 0.5879
3.2430 2.2621 69.75 0.4495
 HPLC %EE

Standard CBD 95% solution 1

Conc (mg/ml) Pipet Stock (ml) to V Conc Std (ug/ml) Pipet Stock (ml)
0.5 5 10 0.5
1 10 10 1 0.01
2.5 25 10 2.5 0.025
A 5 50 10 5 0.05
B 7.5 75 10 7.5 0.075
C 10 100 10 10 0.1

Stock (mg/ml) 5 25 mg 5 ml
D 15 0.15
E 20 0.2
F 25 0.25
G 30 0.3

Peak Area
Conc (ug/ml) 1 2 3 Average SD
1
2.5
5
A 7.5 323783 322463 323805 323350.3333 768.5319338
B 10 439307 434125 431124 434852 4139.658078
C 15 659103 648883 648421 652135.6667 6038.30782
D 20 877079 867719 876395 873731 5217.765039
E 25 1092868 1084470 1098088 1091808.667 6870.52555
F
G
H

Sample CLSE-KP CBD content

KP (mg) CSLE (mg) %Glycine to V (ml) conc CSLE (mg/ml) CBDcontent

1 200 10 1 4 2.50 3.2430
2 200 12 1 4 3.00 3.8916
3 200 14 1 4 3.50 4.5402
200 15 1 4 3.75 4.8645
4 200 16 1 4 4.00 5.1888
5 200 10 1 4 2.50 3.2430
6 200 10 2 4 2.50 3.2430
7 200 10 4 4 2.50 3.2430
 7-Nov 17-Nov
Kp HS15 CSLE No glycine No glycine Glycine 1%
200 10 %EE 89.76 78.02 70.73
Drug content 1.6 1.2449 1.1286
mg/ml อุปกรณ์
1. Volumetric flask 10 ml 22 ขวด
ul to V 2. Micropipet 10 ul
10 10 3. HPLC vial 22 ขวด
10 10
25 10 สารเคมี
50 10 1. Acetonitrile
75 10 2. MilliQ water
100 10 3. Methanol

150 10
200 10
250 10 STD CBD
300 10 1200000

1000000 f(x) = 43899.5869918699 x − 5268.06504065055

R² = 0.999989793529846
800000
Peak area

600000 Column J
Linear (Colum
400000
7.5 323350.3333
200000
10 434852
15 652135.6667 0
6 8 10 12 14 16 18 20 22 24 26
20 873731
25 1091808.667 Conc

32.43
Peak Area
Pipet (ml) ul to V (ACN) Dilute (mg/ml) ug/ml 1 2 3
0.1 100 5 0.050 50.00 500004 490842 493093
0.1 100 5 0.060 60.00 643492 635227 638231
0.1 100 5 0.070 70.00 673029 674938 668334
0.1 100 5 0.075 75.00 834406 819038 814999
0.1 100 5 0.080 80.00 859300 847415 844175
0.1 100 5 0.050 50.00 545648 540481 544088
0.1 100 5 0.050 50.00 595145 588645 590391
0.1 100 5 0.050 50.00 576984 579521 584598
 30-Nov
Glycine 1% Glycine 1% Glycine 2% Glycine 4%
70.23 77.08 83.82 82.27
1.12 1.23 1.34 1.31
8.06504065055

Column J
Linear (Column J)

26

rea
Average Conc CBD ug/ml Conc CBD mg/ml *Dilu mg %EE Drug Content
494646.33 11.39 0.0114 2.2775 70.23 1.12
638983.33 14.68 0.0147 2.9351 75.42 1.44
672100.33 15.43 0.0154 3.0860 67.97 1.51
822814.33 18.86 0.0189 3.7726 77.55 1.84
850296.67 19.49 0.0195 3.8978 75.12 1.90
543405.67 12.50 0.0125 2.4997 77.08 1.23
591393.67 13.59 0.0136 2.7183 83.82 1.34
580367.67 13.34 0.0133 2.6680 82.27 1.31
 HPLC %EE

Standard CBD 95% solution 1

Conc (mg/ml) Pipet Stock (ml) to V Conc Std (ug/ml)

0.5 5 10 0.5
1 10 10 1
2.5 25 10 2.5
A 5 50 10 5
B 7.5 75 10 7.5
C 10 100 10 10

Stock (mg/ml) 5 25 mg 5 ml
D 15
E 20
F 25
G 30
50
100
150

Peak Area
Conc (ug/ml) 1 2 3 Average
1 1.692 1.669 1.9029 1.7546
2.5 3.6354 3.5798 3.6051 3.6068
5 6.8538 6.8737 6.9004 6.8760
7.5 12.0484 14.4552 13.557 13.3535
10 18.4364 17.5534 18.1063 18.0320
15 23.1728 23.1669 23.9317 23.4238
20 28.7025 29.2963 29.3187 29.1058
25 36.1742 37.2943 36.7184 36.7290
50 78.3112 79.1557 80.9458 79.4709
100 151.2901 151.7967 152.3335 151.8068
150 219.694 219.848 222.2304 220.5908

Sample CLSE-KP CBD content

KP (mg) CSLE (mg) %Glycine to V (ml) conc CSLE (mg/ml)

Sam 1 200 15 1 4 3.75
Sam 2 200 15 1 4 3.75
Sam 3 200 15 1 4 3.75
 Peak area
Sample Sampling 1 2 3
Drug release 30 5.4846 5.3956 5.3872
60 21.867 19.7584 19.7925
90 38.8528 39.0506 39.2597
120 42.6519 42.5279 42.5654
Micelle 5.5 3 hr 72.8211 68.7704 68.708
6 hr 103.097 100.893 99.8441
9 hr 106.9579 108.1514 107.0546
12 hr 102.5742 102.4892 102.6408
24 hr 106.6 103.3446 104.5725
30 2.4868 2.3556 2.481
60 16.5617 16.8213 17.4294
90 23.9759 23.3488 24.0912
120 38.7068 37.9131 37.7162
Micelle 7.4 3 hr 60.8882 59.1262 57.9008
6 hr 89.4457 85.2802 84.0442
9 hr 91.6606 92.2222 92.6736
12 hr 93.8013 91.6506 93.8749
24 hr 84.2455 84.8008 84.2873
30 2.6914 2.546 2.7134
60 4.5229 3.9063 4.0305
90 4.9815 4.6088 4.5671
120 5.0984 5.5092 5.5276
CSLE 5.5 3 hr 6.6927 6.7428 6.2779
6 hr 7.8653 7.9006 7.5507
9 hr 8.0051 8.1538 8.0633
12 hr 7.8275 7.8184 7.7449
24 hr 8.6447 8.5314 8.6586
30 2.4246 1.8195 1.4553
60 5.6511 3.9493 3.987
90 4.9774 4.9512 4.9595
120 5.012 4.9379 4.2192
CSLE 7.4 3 hr 6.5029 6.413 5.9761
6 hr 8.0564 6.8507 6.8278
9 hr 7.916 7.1511 7.5465
12 hr 7.9115 7.1093 8.2078
24 hr 9.0727 8.2006 8.2216

Stability
Day0 Day7
Peak area 1 24.0547
Peak area 2 23.9088
Room temp Sample4
Peak area 3 24.3145
Room temp Sample4

Average 0 24.092666667
Peak area 1 26.3351
Peak area 2 26.4257
Cool temp Sample5
Peak area 3 26.6127
Average 0 26.457833333
 mg/ml 5.2 mg อุปกรณ์
1. Volumetric flask 10 ml 22 ขวด
Pipet Stock (ml) ul to V 2. Micropipet 10 ul
10 10 3. HPLC vial 22 ขวด
0.005 5 5
0.0125 12.5 5 สารเคมี
0.025 25 5 1. Acetonitrile
0.0375 37.5 5 2. MilliQ water
0.05 50 5 3. Methanol

0.075 75 5
0.1 100 5
0.125 125 5
Chart Title
0.15 150 5
0.25 250 5 250.0000

0.5 500 5 f(x) = 1.48080844976555 x + 1.19617621

200.0000
0.75 750 5 R² = 0.999052760595532

150.0000

SD Axis Title
100.0000
0.128916653 1 1.7546
0.027837445 2.5 3.6068 50.0000

0.023382543 5 6.8760
0.0000
1.216232121 7.5 13.3535 0 20 40 60 80 100 120 140 160
0.446160177 10 18.0320
Axis Title
0.439864195 15 23.4238
0.349476427 20 29.1058
0.560124757 25 36.7290
1.345285312 50 79.4709
0.521772837 100 151.8068
1.422021491 150 220.5908

32.43 Result
Peak Area
CBDcontent Pipet (ml) ul to V (ACN) Dilute (mg/ml) ug/ml 1 2
4.8645 0.1 100 5 0.075 75.00 34.4713 34.6852
4.8645 0.1 100 5 0.075 75.00 37.1573 37.9845
4.8645 0.1 100 5 0.075 75.00 34.2335 34.1622
 Average Conc CBD (ug/ml) mg/ml 30
5.422466667 2.85 0.00285 60
20.47263333 13.02 0.01302 90
39.05436667 25.57 0.02557 120
42.58173333 27.95 0.02795 180
70.09983333 46.53 0.04653 240
101.2780333 67.59 0.06759 540
107.3879667 71.71 0.07171 720
102.5680667 68.46 0.06846 1440
104.8390333 69.99 0.06999 30
2.441133333 0.84 0.00084 60
16.93746667 10.63 0.01063 90
23.8053 15.27 0.01527 120
38.11203333 24.93 0.02493 180
59.30506667 39.24 0.03924 240
86.2567 57.44 0.05744 540
92.18546667 61.45 0.06145 720
93.10893333 62.07 0.06207 1440
84.44453333 56.22 0.05622 30
2.650266667 0.98 0.00098 60
4.153233333 2.00 0.00200 90
4.719133333 2.38 0.00238 120
5.3784 2.82 0.00282 180
6.571133333 3.63 0.00363 240
7.7722 4.44 0.00444 540
8.074066667 4.64 0.00464 720
7.796933333 4.46 0.00446 1440
8.611566667 5.01 0.00501 30
1.8998 0.48 0.00048 60
4.529133333 2.25 0.00225 90
4.9627 2.54 0.00254 120
4.723033333 2.38 0.00238 180
6.297333333 3.44 0.00344 240
7.244966667 4.08 0.00408 540
7.537866667 4.28 0.00428 720
7.742866667 4.42 0.00442 1440
8.4983 4.93 0.00493

Conc CBD (ug/ml)

Day14 Day0 Day7 Day14
15.52
0

0
 Chart Title

8080844976555 x + 1.19617621731808
9052760595532

Column K
Linear (Column K)

60 80 100 120 140 160

Title

Result
Peak Area
3 Average Conc CBD ug/ml Conc CBD mg/ml *Dilu mg %EE Drug Content
35.0614 34.74 22.65 0.0227 4.5304 93.13 2.21
39.2524 38.13 24.94 0.0249 4.9885 102.55 2.44
34.4814 34.29 22.35 0.0224 4.4700 91.89 2.18

Chart Title
120

100
 Chart Title
5.422466667
20.47263333 120

39.05436667 100
42.58173333
80
70.09983333

Axis Title
101.2780333 60 Column P
107.3879667
40
102.5680667
104.8390333 20
2.441133333
0
16.93746667 0 200 400 600 800 1000 1200 1400 1600
23.8053 Axis Title
38.11203333
59.30506667
86.2567
92.18546667 Chart Title
93.10893333 100
84.44453333 90
2.650266667 80
70
4.153233333
60
4.719133333 50
5.3784 40
6.571133333 30
7.7722 20
10
8.074066667
0
7.796933333 0 200 400 600 800 1000 1200 1400 1600
8.611566667 Column P
1.8998
4.529133333
4.9627
4.723033333
Chart Title
6.297333333
10
7.244966667
9
7.537866667
8
7.742866667
7
8.4983
6
5
4
3
2
1
0
0 200 400 600 800 1000 1200 1400 1600

Column P
 Chart Title
9
8
7
6
5
4
3
2
1
0
0 200 400 600 800 1000 1200 1400 1600

Column P