Concentration of Ach No atropine control Atropine 1X10-09 (M) Atropine 3X10-08 (M)

1.00E-09 0 0 0
3.00E-09 0 0 0
1.00E-08 10 2 0
3.00E-08 29 16 0
1.00E-07 46 33 1
3.00E-07 54 48 10
1.00E-06 56 56 30
3.00E-06 60 59 46
1.00E-05 58 60 54
3.00E-05 60 60 57
1.00E-04 60 60 59
3.00E-04 60 60 60
1.00E-03 60 60 60
3.00E-03 59 60 60
1.00E-02 60 60 60
3.00E-02 60 60 60
Atropine 1X10-06 (M) control 2.00E-08
0 atropine 1 7.00E-08
0 atropine 2 1.00E-05
0 atropine 3 2.00E-04
0
0
0 Action of the antagonist atropine against ACh concentration
0
70
0
10
29
43 60
54
58
59
59 50
60
Amplitude of response (A.U)

40

30

20

10

0
1.00E-09 1.00E-08 1.00E-07 1.00E-06 1.00E-05 1.00E-04 1.00E-03 1.00E

Log(ACh)

No atropine control Atropine 1X10-09 (M) Atropine 3X10-08 (M) Atropine

 this will be x-axis
Atropine conc. Log10(atropine conc)
no atropine 0
1X10-09 -9
3X10-08 -7.52
ACh concentration 1X10-06 -6

Schild plot for the concent

3.5

2.5
Log10(DR-1)

1.5

0.5

0
-10 -8
Log(Atropine conc.

E-04 1.00E-03 1.00E-02 1.00E-01

X10-08 (M) Atropine 1X10-06 (M)

 this will by y axis
EC50 DR DR-1 Log10(DR-1)
3.00E-08 0 0 0
8.00E-08 2.66 1.66 0.22
1.00E-06 33.3 32.3 1.51
2.00E-05 1000 999 2.99

Log10(atropine conc) Log10(DR-1)

-9 0.22
-7.52 1.51
-6 2.99

Schild plot for the concentration of atropine

-8 -6 -4
Log(Atropine conc.)
Distance (μm) voltage (mV)
20 7.6
500 3.8
1000 1.8
1500 1

Length constant graph

8
7
6
5
voltage (mV)

4
3
2
1
0
0 200 400 600 800 1000 1200 1400 1600
distance
concentration of Ach(M) amplitude of response (A.U)
1.00E-10 0
3.00E-10 0
1.00E-09 0
3.00E-09 3
1.00E-08 13
3.00E-08 31
1.00E-07 45
3.00E-07 57
1.00E-06 58
3.00E-06 60
1.00E-05 59
3.00E-05 58
1.00E-04 60
3.00E-04 60
1.00E-03 59
3.00E-03 60
1.00E-02 59
3.00E-02 60

EC50 0.01 microM

threshold
 Guinea-pig ileum cont
70

60

50
amplitude of response (A.U)

40

30

20

10

0
1.00E-10 1.00E-08 1.00E-06
 0
1.00E-10 1.00E-08 1.00E-06
Guinea-pig ileum contraction with the concentration of ACh

1.00E-06 1.00E-04 1.00E-02

concentration of ACh (M)

1.00E-06 1.00E-04 1.00E-02

concentration of ACh (M)

1.00E-02 1.00E+00
1.00E-02 1.00E+00
 Amplitude of response (A.U)
Concentration of Ach No atropine control Atropine 1X10-09 (M) Atropine 3X10-08 (M)
1.00E-09 0 0 0
3.00E-09 0 0 0
1.00E-08 10 2 0
3.00E-08 29 16 0
1.00E-07 46 33 1
3.00E-07 54 48 10
1.00E-06 56 56 30
3.00E-06 60 59 46
1.00E-05 58 60 54
3.00E-05 60 60 57
1.00E-04 60 60 59
3.00E-04 60 60 60
1.00E-03 60 60 60
3.00E-03 59 60 60
1.00E-02 60 60 60
3.00E-02 60 60 60
U)
Atropine 1X10-06 (M)
0
0
0
0
0
0
0
0
10
29
43
54
58
59
59
60
concentration of DAMGO % inhibition of ileum contraction
1.00E-08 60
3.00E-08 85
1.00E-07 91
3.00E-07 92
1.00E-06 91

%
95

90

85

% inhibition of ileum contraction

80

75

70

65

60

55
1.00E-09
 % inhibition of nerve-evoked response of DAMGO at different concentration
95

90

85

80

75

70

65

60

55
.00E-09 1.00E-08 1.00E-07 1.00E-06

concentration of DAMGO [M]

at different concentration

1.00E-06 1.00E-05

]
DAMGO ( 1 µM) 0.05
DAMGO (1 µM ) + Naloxone (300nM) 0.184
 The effect of DAMGO when Naloxone is added
0.2

0.18

0.16

0.14

0.12
Contraction response

0.1

0.08

0.06

0.04

0.02

0
DAMGO ( 1 µM) DAMGO
oxone is added

DAMGO (1 µM ) + Naloxone (300nM)