- FLUORESCENT FLOW CYTOMETRY

CBC: COMPLETE BLOOD COUNT RETICULOCYTE

- SYSMEX 550 : main machine - RATIO: 4:3 (reticulocyte stain :
- sampler and manual blood sample)
- EDTA tube - Red top tube
- Inversion: 8 – 10 - 15 mins
- SYSMEX 350: back up machine - Mix gently
- Manual only for rerun of sample - Smear
(hgb, hct, rbc, wbc, plt, nue, leu, - 10 fields/1000 x 100 = n %
eo,baso) - Normal value: 0.5% - 2.5%

PBS: PERIPHERAL BLOOD SMEAR ESR: ERYTHROCYTE SEDIMENTATION RATE

- Kapag mababa ang platelet ni - Westergren Method
patient sa result kailangan ismear - Inflammation
- Platelet count - ESR pipette, black top tube,
- 10 fields x 2 = n pomelo top tube
STAINING: - 60 min or 1hr
- Wright staining - Unit : mm/hr
- Acid alcohol (3 dips) - Normal value:
- Diff quick I (10 dips) - Men: 15 – 20 mm/hr
- Diff quick II (10 dips) - Female: > 20 mm/hr
- Water (1 dip)
- Pwede after DQ 1 wash water
and DQ 2. Pwede din kahit sa last HEMATOLOGIST
na ang water. - DRA. Aimee Elaine Ausa (pedia)
- Stay ang slide bilang nalang ng - DRA, Clarisse May Pascua
seconds para hindi ma wash ang (adult)
smear
- Normal value : 150,000 – 450,000

APTT: ACTIVATED PARTIAL

THROMBOPLASTINE TIME
PT: PROTHROMBIN TIME
- SYSMEX 400: manual encode
- Citrate tube/ Blue top
- 5 inversion
- 15 min centri
- Check the reagents before
running.
- Check pt control range for the
result
- Pt normal Value: 10 – 13 seconds