Sweat test

A sweat test measures the amount of chloride in sweat. Chloride is part of the salt that's
found in sweat. The test can diagnose cystic fibrosis (CF) because people with CF have
higher levels of chloride in their sweat. CF is an inherited disease that can affect people
of all ages. To have cystic fibrosis, you must inherit a changed CFTR gene from both
your parents. (Changes in genes are also called variants or mutations.) If you inherit
one changed gene, you won't have the disease, but you will be a carrier. Being a carrier
means that if you have children with someone who also has changes in one or both of
their CFTR genes, your children could inherit two changed genes and have CF.

PROCEDURE
1. Selectanarea
2. Apply iodine in designated area
3. Put a starch
4. Wait a atleast 20 mins for the result

Starch test in serum/plasma

The iodine test for starch is mainly performed to test the presence of carbohydrates.
The food products which we eat include different types of carbohydrates, among which
starch and sugars are the main carbohydrates found in our food products.

PROCEDURE
1. Extract blood
2. Wait for the serum/plasma to settle
3. Transfer the serum to two tube
4. Tube A will be having the 0.5 ml starch solution 2-3 drops of iodine
5. Tube B will be having no starch solution 2-3 drops of iodine

Post question

1. What is the main purpose of the starch test in the sweat glands?
-In order to do a starch test, you must wet a portion of your body—usually your palm—
with iodine and then sprinkle starch powder over it. The primary goal of a starch test
performed on the sweat glands is to locate the area of the skin where persistently high
sweating occurs.

2. Why do I need a sweat test?

-The most used method of diagnosing cystic fibrosis is sweat testing. People with cystic
fibrosis had greater levels of salt and chloride in their perspiration, according to the test.
Some individuals are tested because they exhibit symptoms.
3. What happens during a sweat test?
-In order to do a sweat test, we first identify which hand or palm is perspiring
excessively. We next wet the palm with iodine and sprinkle starch powder on it.
Following those steps, we carefully watched how sweat reacted with iodine and starch.
We discovered that, depending on how bad the sweating was, the starch would turn
black, purple, or brown.

4. What is the indicator if there is a carbohydrate in the serum/plasma?

-Iodine and starch will show if there is a carbohydrate present in the serum or plasma. A
result of having black, white, and gray can be obtained by combining the serum or
plasma with iodine and starch. A serum containing starch and iodine that becomes
black indicates a positive result; a serum that turns white indicates a negative result,
indicating that there are no carbohydrates present in either the serum or the plasma.

5. How does the reaction of the test in starch occur?

-My partner and I begin the starch test by absorbing serum or plasma from the purple or
EDTA tube, which already has the plasma and red blood cells separated. Next, we put
roughly ten drops of serum into a red tube, and once we've added all ten drops, we
combine the serum with our professor's starch solution. Therefore, we added two to
three drops of iodine along with the serum and starch solution, and we continued to
shake the tube in a circular motion. Following that, we noticed that the serum's color
changed, turning it gray as a result. The serum turns gray because it contains a lot of
carbohydrates, which led us to conclude that the blood's owner also consumes a lot of
foods high in carbohydrates.

6. What are the diseases that can be detected by using a starch test?
-A starch test can be used to identify or diagnose certain diseases, such as Horner
syndrome. Clinical signs of the syndrome include ipsilateral drooping eyelids,
decreased sweating on the afflicted side of the body, and decreased pupil size, which is
worsened in scotopic conditions. In addition, the iodine-starch test is an excellent
diagnostic tool for Horner syndrome.