Materi Ten

T.E.
www.ten-korea.com
2008. 03 2010. 01 2012. 01 2014 .03 2015. 04 2015. 11
Business area
TEN develops facial mask essences based on natural raw materials,
self-developed natural plant extract and natural preservative system.
“We can supply customized solutions for you as well.“
Mask
BIO R&D Center
BIO Research and Development Center
“High Qualified Cosmetic Raw Materials & Formula.“

Patent No. 10-1814065
Melazero ®
More Be Nature
Phyto Whitening agent from
Novel mechanism
Technical Brochure Copyright 2018. Ver 1.2, T.E.N. Co., Ltd.

Melazero General info. Efficacy Safety
Previous Whitener
1st Generation Whitener - (Melanin synthetic (tyrosinase) inhibition)
tyrosinase tyrosinase
tyrosinase
Eumelanin
Mixed Melanins Pheomelanin
Previous Whitener
2nd Generation Whitener - (Melanin synthetic (pathway) inhibition)
Nucleus Melanosome
Tyrosine
DOPA
MITF
Tyrosinase
Dopaquinone
Dopachrome Cysteinyldopa
TYRP2
TYR / TYRP1 / TYRP2
DHI DHICA
TYRP1
Pheomelanin Eumelanin
M Box
Our Innovative Whitener

3rd Generation Whitener - (Created melanin erasing & Melanin synthetic inhibition)
Total Solution for Whitening
Created Melanin Melanin

(Melanophagy) granules
Epidermis
Melanosome
Melanin Synthesis
Basal Layer
Melanocyte

Created Melanin Erasing
‘Autophagy controlling’
‘Autophagy’
2016’s The Nobel Prize in Physiology or Medicine

What is `Autophagy’ ?
is the natural, regulated, destructive mechanism of the cell

‘Autophagy’ that disassembles unnecessary or disfunctional components.
Step 1 Unnecessary cytoplasms are enclosed by phagophore.
Step 2 Autophagosomes are formed and lysosomes bind by autophagosomes.
Step 3 Unnecessary cytoplasms are perfectly disassembled to amino acids, saccharides, etc

What is `Melanophagy’ ? (Autophagy in melanocyte)
Novel pathways for Whitening
Melanophagy mechanism
Melanin
granules
Lysosome
Epidermis
Melanosome
Basal Layer
Melanocyte Autophagosome Melanin Amino acids
Step 1 Melanin granules are enclosed by phagophore.
Step 2 Autophagosomes are formed and lysosomes binds by autophagosomes.
Step 3 Melanin granules are perfectly disassembled to amino acids.


How’s action on melanocyte to pathway inhibition ?
Inhibition pathways for Whitening
Totally inhibition to making melanin

(Both of Pheomelanin and Eumelanin)

Melazero - Origin
Phyto Whitener from Korean Oriental Plant
Deep Forest Wild Plant


Melazero - Manufacturing process
Non chemical purification from Oriental Plant
Extraction Stabilization
Non-chemical High concentrated

purification Phyto Whitener
Arisaema Amurense
Patent No.10-1814065
High Efficacy & Less Price Recommend dosage
1%

Melazero - Composition
Information of Ingredients
INCI name Composition(%) Remarks
Arisaema Amurense Extract 78.0 Plant origin
Decyl glucoside 20.0 Natural origin
1,2-Hexanediol 2.0
More Be Natural !
Enormous Whitening effect

Melazero – Melanophagy controlling
Melanophagy
Controlling

Created Melanin Erasing - Melanophagy controlling
Novel pathways for Whitening
Melanophagy mechanism
Melanin Lysosome
granules
LC3I p62
Epidermis
Melanosome
Basal Layer
LC3II
Melanocyte
Autophagosome Melanin Amino acids
Step 1 Melanin granules are enclosed by phagophore containing LC3I protein.
Autophagosomes are formed ( LC3I protein is conversed to LC3II protein) and

Step 2
lysosomes bind to autophagosomes by p62 protein.
Step 3 Melanin granules are perfectly disassembled to amino acids containing p62 protein.

Created Melanin Erasing - Melanophagy controlling
Melanophagy Inducing on Melanocyte
Inducing Melanophagy Created Melanin Erasing

0.2% 1.0%
Control 1% Melazero 1% α–Arbutin Control
Melazero Melazero
p62
LC3 I
LC3 II
Actin
Cf) 1% α–Arbutin = had no effect on autophagy factors : p62 LC3 II


Melazero - Melanin Synthesis Inhibition
Melanin synthetic
Inhibition

Melanin Synthesis Inhibition
Melanin synthetic inhibition
Cell culture analysis


Melanin Synthesis Inhibition
Melanin Contents Analysis
Melazero Melazero
- +
0.2% 1.0%
Tyrosinase
Actin
α-MSH

Whitening effect - 3D Cell
3D Cell Culture

2% Kojic acid vs 1% Melazero
Control 2% Kojic acid 1% Melazero

Melanin
△L value (0: more white / 20: more black)

Control : 18.5 / Kojic acid 2% : 12.5 / Melazero 1% : 11.8
More Effective than Kojic acid


Control 2% Kojic acid 1% Melazero

Cell Cell death Non toxic
Normal morphology Destroyed morphology Normal morphology
More Safe & Non-toxic than Kojic acid


More 20
Black 18
16
14
Control
△L value
12
10 2% Kojicacid
8 0.2% Melazero
6 1% Melazero
4
2
More
White 0
0 day 3 day 6 day 9 day 12 day
Time
More Effective than Kojic acid


Whitening effect - Clinical Test (Hyperpigmentation)

Clinical Test - Hyperpigmentation
Clinical Test (22 persons, Hyperpigmentation)
M-value (Melanin value)

1%
Melazero
2 weeks later,
Whitening effect confirmed
(p-value <0.001)

Clinical Test - Hyperpigmentation
Clinical Test (22 persons, Hyperpigmentation)
1% 1%
α–Arbutin Melazero
VS
4 weeks later, 2 weeks later,

whitening effect confirmed Whitening effect confirmed
(p-value <0.001)
‘More Rapid Effect than α–Arbutin’

More SAFE
Safety - Clinical Test (Skin Irritation)
Clinical Test (34 persons, Patch test)
2%
Melazero
‘Non-irritation for 24hr’

More SAFE
Safety Test - Cytotoxicity on Melanoma cell
Cell Test (MTS assay, Cytotoxicity)

Cell viability (%)
Don’t worry about “Vitiligo”

Melazero
The Best Partner of You!
www.ten-korea.com T. 82-(0)31-8013-6884 F. 82-(0)31-8060-7336 E. ten-info@naver.com

Patent No. 10-1864203
Mucogen General info. Efficacy Patent
Phyto Mucin
Mucogen
More Be Nature

Phyto Mucin
Mucin & Cosmetics
Snail mucin is the most well-known material in mucin cosmetics so far.

Cosmetic products below are getting popular in Korea and China market.
Animal Mucin
Phyto Mucin
Mucin & Cosmetics
Mucogen is derived from marine plants

The animal experiments for scientific and cosmetic purposes has been regulated since 2010.
Plant Mucin Animal Mucin
VS
What is your choice ?

Phyto Mucin
Mucin & Cosmetics
Mucogen is optimized for moisturizing and anti-aging,

and it is refined by using our own manufacturing process.
Kelp Gulf-weed
Seaweed Enteromorpha
Compressa
Fusiformis
Phyto Mucin
Composition
More Be Natural !
Recommend dosage
Laminaria Japonica Extract 海带（LAMINARIA JAPONICA）提取物 19.6
Undaria Pinnatifida Extract 裙带菜（UNDARIA PINNATIFIDA）提取物 19.6
Sargassum Fusiforme Extract 小叶海藻（SARGASSUM FUSIFORME）提取物 19.6
Sargassum Fulvellum Extract
2-5%
微劳马尾藻（SARGASSUM FULVELLUM）提取物 19.6
Enteromorpha Compressa Extract 扁浒苔（ENTEROMORPHA COMPRESSA）提取物 19.6
1,2-Hexanediol 1,2-己二醇 2.0
Phyto Mucin
Mucin & Cosmetics
When leaves or stems of marine plants get hurt by tide, sand, etc.,
they produce mucus by themselves to prevent penetration of microbes,
the mucus is called ‘Mucin’ or ‘Fucoidan’.
Mucin is a component of skin, cartilage, blood vessel and mucus.

It is a natural moisturizer, which provides elasticity to skin and blood vessel.
It also boosts immune system against harmful factors and has an excellent skin soothing effect.
Phyto Mucin
Moisturizing Effect
Moisturizing Effect
Moisturizing Effect
40.0
35.0
Moisture(A.U.)
30.0
25.0
20.0
5min 10min 15min 30min 1hr 2hr
Time
Control Marin Mucin-DS

Mucogen 5%
Mucogen has excellent effect on moisturizing.

Phyto Mucin
Elasticity Effect
Elasticity Effect
Elasticity Effect
100.0
80.0
Elasticity(A.U.)
60.0
40.0
20.0
0.0
5min 10min 15min 30min 1hr 2hr
Time
Control Mucogen 5%
Marin Mucin-DS
Mucogen shows great effect on improving skin firming.

Phyto Mucin
Improvement of skin roughness : Skin topography
Before After (3 hour)
2D image 3D image 2D image 3D image
Picture
Figure
Phyto Mucin
Patent
Patent number : 10-1864203
Mucin derived from Marine plants, the manufacturing method and cosmetic composition containing it
Mucin derived from Marine plants, through our

unique manufacturing process, can display a wide
range of biological activities including anti-oxidation,
anti-irritation, anti-wrinkle, and anti-inflammatory
activities. It also can be used in the products to
alleviate atopic dermatitis.
Mucogen

Patent No. 10-1837020
Bubble Berry ®
More Be Nature
Phyto Foam Booster

Bubble Berry General info. Efficacy Safety
Phyto Foam Booster
Origin
Phyto foam booster from Soapberry
Indian Area
Phyto Foam Booster
Soapberry
Information
Sapindus mukorossi is a species of tree in the Sapindaceae family.

The fruit is commonly known as Chinese soapberry or washnut, and like
other species in the genus Sapindus, it is called soapberry.
It is also a native of Western coastal Maharashtra - Konkan, and Goa in
India. Sapindus mukorrossi, known as the ritha or reetha tree in Nepal,
is a deciduous tree that is grown in the lower foothills and midhills of the
Himalayans, up to altitudes of 4000 feet.
Soapberry Effects
Also fruit saponin is known as pharmaceutical controller on

pimples, eczema, migranes, psoriasis.
Phyto Foam Booster
Manufacturing process
Non chemical purification from Soapberry
Extraction Stabilization
Non-chemical High concentrated

purification Phyto Foam Booster
Patent No. 10-1837020
Odor Efficacy
Color Less More Stable
Price Easy for use
Recommend dosage
1–5%
Phyto Foam Booster
Composition
More Be Natural !
INCI name CFDA name Composition (%)
Sapindus Mukorossi Fruit Extract 无患子(SAPINDUS MUKUROSSI)果提取物 98.0

Phyto Foam Booster
Stability Test
Stability (5% in Water)
T=0 T=10 min T=1 hour T=1 day
Natural & Synthetic surfactant

From Apple amino
ino acids
acids
vs Sedimentation
Bubble Berry T=0 T=10 min T=1 week T=1 month
More Stable !
~ 100 % easy soluble and stable in water
Phyto Foam Booster
Solubility Test
Solubility (5% in Water)
Apple amino acids Bubble Berry

Natural & Synthetic surfactant
From apple
Apple amino
amino acids
acids
vs
Bubble Berry
More Transparent !
Transparent formation in water
Phyto Foam Booster
Foaming Test
1% 2% 3% 4% 5%
Bubble Berry
in water in water in water in water in water
Product
T=0
(24℃, RH 22%)
T=6 hr
(24℃, RH 22%)
Maintainous & Stable foaming on very low humidity

Phyto Foam Booster
Microbial Washing Test
Microbial washing test on S. aureus (5 x 106 cfu)
Control Water 5% Bubble Berry in Water
Loading microbial 100ml wash One pumping wash
5 x 106 cfu 3 x 104 cfu 1 x 103 cfu
99.98% Reducing !
Microbial washing (Effectual elimination )
Phyto Foam Booster
Minimum Inhibitory Concentration Test
Minimum Inhibitory Concentration Test on C. albicans (1 x 105 cfu/ml)
MIC
Phyto Foam Booster
Antioxidative Effect Test
Concentration dependent increasing

Phyto Foam Booster
Clinical Test (Skin Irritation)
10%
Bubble Berry

Bubble Berry

Phytomoist
More Be Nature
Phyto Moisturizer

Phytomoist General info. Efficacy Safety
Natural Moisturizer
Origin
Phyto Moisturizer from Asia area Plantago asiatica seed
Asia
Natural Moisturizer
Plantago asiatica seed
Information
Plantago asiatica is a species of the Plantaginaceae family.

In Korea, young seeds are eaten as side dish. And seeds are sun dried
and used as medicines. Dried seeds have diuretic action, stop diarrhea,
and activate liver function.
It grows really well in disturbed areas like roadsides or even dirt roads.
So it called symbol of persistent vital.
Effects
Plantago asiatica Seed This plant was traditionally used in Asian pharmacopoeia for the
treatment of liver disease. It has also been used to treat stomach
troubles and inflammation of the bladder and urinary tract.
Its seed has a mild laxative effect.
Natural Moisturizer
Composition
More Be Natural !

Recommend dosage
Plantago Asiatica Seed Extract 车前(PLANTAGO ASIATICA)籽提取物 67.0
Dipropylene glycol 双丙甘醇 30.0

1–5%
Natural Moisturizer
Moisturizing Effect Test - in vivo
More Moisturizing !
40.0
Control
Phytomoist Base(without active) 5%
Phytomoist 5%
35.0
Moisture(A.U.)
30.0
25.0
control 5min 10min 15min 30min 1hr 2hr 3hr
Time
Natural Moisturizer
Skin statement recovery
Phytomoist Base
Before (without active) 5%
Phytomoist 5%
2D image
3D image
Skin surface
Irregular Irregular Flat

Natural Moisturizer
Moisturizing Effect Test - in vivo
Moisturizing effect : compared with β-Glucan
β-Glucan
vs
Phytomoist
Natural Moisturizer
Moisturizing Effect - Clinical Test (TEWL & Hydration)
Natural Moisturizer
Clinical Test – 11 persons
Moisturizing effect
Improving skin barrier function !
Transepidermal Water Loss Improving rates of TEWL

14 35% 31.51%
Control Control
30%
13
Phytomoist 5% Phytomoist 5%
TEWL (g/m2/hr)
25%
12 Control
20% 18.35%
11 vs 15.08%
15%
10 Phytomoist
10% 7.19%
9 5%
0% 0%
8 0%
Before 2weeks 4weeks Before 2weeks 4weeks
Natural Moisturizer
Clinical Test – 11 persons
Moisturizing effect
Improving skin hydration function !
Water content of stratum corneum Improving rates of hydration

55
40% 37.21%
Control 50.92 Control
50
30%
Corneomter value
Control
Rates of increase
Phytomoist 5% Phytomoist 5%
20.39%
45 43.74 vs 17.86%
20%
44.49 Phytomoist
8.51%
40 10%
37.11 40.1
0% 0%
35 36.95 0%
Before 2weeks 4weeks Before 2weeks 4weeks
Natural Moisturizer
Clinical Test (11 persons, Long-term test)
5%
Phytomoist
‘Non-irritation for 4weeks’

Phytomoist

Calnomix
More Be Nature
Phyto Anti-inflammation agent

General info. Efficacy Safety
Calnomix
Our Anti-inflammation agent
Origin
Phyto Anti-inflammation complex from Europe, Asia, America Plant
From Eco-friendly Field, Forest, Mountain

Calnomix
Anti-Inflammation
Anti-inflammation effect
Inclusive skin-soothing solution
Maple Syrup Chamomile Portulaca

Moisturizing Anti-Irritation Anti-inflammation
Calnomix
Our Anti-inflammation agent
Composition
30.0
40.0
More Be Natural !
15.0
15.0

Recommend dosage
Chamomilla Recutita Flower Extract 母菊(CHAMOMILLA RECUTITA)花提取物 30.0
Acer saccharum Extract 糖槭(ACER SACCHARUM)提取物 15.0

2–3%
Portulaca Oleracea Extract 马齿苋(PORTULACA OLERACEA)提取物 15.0
Butylene glycol 丁二醇 40.0
Calnomix
Anti-Inflammation
Anti-inflammation (Summary pathway)
Extracellular
Inflammatory insult
MAPKs P38 and JNK

Calnomix
Pro-inflammatory Inflammatory Response

cytokines (IL-1, IL-6, etc)
Calnomix
Anti-Inflammation
Anti-inflammation Effect (IL-1)
Expression of IL-1 alpha (%)
Reducing of
Pro-inflammatory
Cytokine IL-1
Calnomix(%)
UVB(10mj/㎠)
Calnomix
Anti-Inflammation
Anti-inflammation Effect (IL-6)
Expression of IL-6 (%)
Reducing of
Pro-inflammatory
Cytokine IL-6
Calnomix(%)
UVB(10mj/㎠)
Calnomix
SAFETY
10%
Calnomix

Calnomix

Materi Ten

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T.E.

“We can supply customized solutions for you as well.“

“High Qualified Cosmetic Raw Materials & Formula.“

Technical Brochure Copyright 2018. Ver 1.2, T.E.N. Co., Ltd.

Our Innovative Whitener

Total Solution for Whitening

Created Melanin Melanin

Our Innovative Whitener

Our Innovative Whitener

is the natural, regulated, destructive mechanism of the cell

Step 1 Unnecessary cytoplasms are enclosed by phagophore.

Step 2 Autophagosomes are formed and lysosomes bind by autophagosomes.

Our Innovative Whitener

Novel pathways for Whitening

Melanocyte Autophagosome Melanin Amino acids

Step 1 Melanin granules are enclosed by phagophore.

Step 2 Autophagosomes are formed and lysosomes binds by autophagosomes.

Step 3 Melanin granules are perfectly disassembled to amino acids.

Our Innovative Whitener

Inhibition pathways for Whitening

Totally inhibition to making melanin

Our Innovative Whitener

Phyto Whitener from Korean Oriental Plant

Deep Forest Wild Plant

Our Innovative Whitener

Non chemical purification from Oriental Plant

Non-chemical High concentrated

High Efficacy & Less Price Recommend dosage

Our Innovative Whitener

INCI name Composition(%) Remarks

Arisaema Amurense Extract 78.0 Plant origin

Decyl glucoside 20.0 Natural origin

Enormous Whitening effect

Enormous Whitening effect

Novel pathways for Whitening

Step 1 Melanin granules are enclosed by phagophore containing LC3I protein.

Autophagosomes are formed ( LC3I protein is conversed to LC3II protein) and

Enormous Whitening effect

Melanophagy Inducing on Melanocyte

Inducing Melanophagy Created Melanin Erasing

Cf) 1% α–Arbutin = had no effect on autophagy factors : p62 LC3 II

Enormous Whitening effect

Enormous Whitening effect

Melanin synthetic inhibition

Cell culture analysis

Enormous Whitening effect

Melanin Contents Analysis

Enormous Whitening effect

Enormous Whitening effect

2% Kojic acid vs 1% Melazero

Control 2% Kojic acid 1% Melazero

△L value (0: more white / 20: more black)

More Effective than Kojic acid

Enormous Whitening effect

2% Kojic acid vs 1% Melazero

Control 2% Kojic acid 1% Melazero

Normal morphology Destroyed morphology Normal morphology

More Safe & Non-toxic than Kojic acid

Enormous Whitening effect

2% Kojic acid vs 1% Melazero

More Effective than Kojic acid

Enormous Whitening effect

Enormous Whitening effect

Clinical Test (22 persons, Hyperpigmentation)