FOR 1L BBM Media

# COMPONENT AMOUNT STOCK SOLUTION CONC.

1 NaNO3 10ml 10g/400ml
2 CaCl2.2H2O 10ml 1g/400mL
3 MgSO4.7H2O 10ml 3g/400ml
4 K2HPO4 10ml 3g/400mL
5 KH2PO4 10ml 7g/400mL
6 NaCl 10ml 1g/400ml
7 EDTA Stock 0.05ml
8 Iron Stock 0.05ml
9 Boron Stock 0.05ml
10 Bold Trace Stock 0.05ml

EDTA Stock 1L
1 dH2O 900mL
2 EDTA 50g
3 KOH 31g
Bring water to 1L

Iron Stock 1L
1 dH2O 950mL
2 H2SO4 10mL
3 FeSO4.7H2O 5g
Bring water to 1L

Boron Stock 1L
1 dH2O 900mL
2 H3BO 11g
Bring water to 1L

Bold Trace Stock 1L

1 dH2O 900mL
2 H2SO4 conc. 10mL
3 ZnSO4.7H2O 8.82 g/L 0.44 mg/L
4 MnCl.4H20 1.44 g/L 0.072 mg/L
5 MoO3 0.71 g/L 0.38 mg/L
6 CuSO4.5H2O 1.57 g/L 0.78 mg/L
7 Co(NO3)2.6H2O 0.49 g/L 0.025 mg/L
Bring water to 1L
 For 100 ml BBM Media
AMOUNT STOCK SOLUTION CONC.
1ml 2.5g/100ml
1ml 0.25/100ml
1ml 0.75g/100ml
1ml 0.74g/100ml
1ml 1.75g/100ml
1ml 0.25g/100ml
0.005ml
0.005ml
0.005ml
0.005ml

EDTA stock 100ml

90ml
5g
3.1g
Bring water to 100ml

Iron Stock 100ml

95ml
1ml
0.5g
Bring water to 100ml

Boron Stock 100ml

90ml
1.1g
Bring water to 100ml

Bold Trace Stock 100ml

90ml
1ml
0.882g 0.044mg/100ml
0.144g 0.0072mg/100ml
0.071g 0.038mg/100ml
0.157g 0.078mg/100ml
0.049g 0.049mg/ml
Bring water to 100ml
COMPONENT QUANTITY

NaNO3
CaCl2.2H2O
MgSO4.7H2O
K2HPO4
KH2PO4
NaCl 500g

EDTA
KOH 500g

H2SO4
FeSO4.7H2O 500g

H3BO 500g

H2SO4
ZnSO4.7H2O
MnCl.4H20
MoO3
CuSO4.5H2O
Co(NO3)2.6H2O 500g