BLOOD BANK RATIO 18.

located on Aquaporin 1 channel forming Integral protein

(GHIP): Colton
1. tissues where ABO is found: Kidney, Heart, Bone, Pancreas
19. gene carrying structure composed of: Chromosome
2. age of gestation for ABO antigen: 5-6 weeks
20. chromosome w/c male & female : Homologous (sex
3. ABO chromosome: 9q34
chromosome)
4. A to B-like analog enzyme: Deacetylase
21. not a sex chromosome: Autosomes
5. true of Anti-A: Can be found of sera of weak A
22. condensation of chromatin: Prophase
6. most important after ABO: Rh
23. donor took feldene (like aspirin) yesterday, deferral: 2 days
7. RHD gene location: Chromosome 1
24. can donate today: Female donor w/ the temp of 36’C
8. Rh gene regulator: RHAG
25. Hgb for autologous donation: 11g/dL
9. Rh ag second most immunogenic: c
26. 6 months deferral: Avodart
10. true of Weak D: I. Defined on having a reduced amount of
27. deferral for experimental vaccines: 12 months
D; II. DAT- HTR, HDN, AIHA;
28. 2 weeks deferral EXCEPT: 3 of these (rubeola, rubella &
III. IAT- weak D
oral typhoid: 1 month DOH)
11. caused delay transfusion reaction EXCEPT: Duffy
29. deferral for old components: 12 months
12. African ethnicity : Jsa
30. hepatitis @ age 9: No deferral
13. receptor for merozoites of P. vivax: Duffy
31. med for prostate gland enlargement: Proscar & Avodart
14. chemokines for Duffy antigen receptor EXCEPT: IL-5
32. med for baldness: Finasteride
15. true of Jk antibody: Jk-a and Jk-B: Not common but are
33. pH of ACD-B: 4.5-5.5
commonly found in mixtures; Usually
34. CP2D : 5.3-5.9
IgG1 & IgG3 16. Ag located on RC Urea Transporter: Kidd
35. CPD/CPDA1 : 5.0-6.0
17. destroys Diego Antigen, EXCEPT: Ficin, Papain, Trypsin (3
of these) 36. ACD-A/-B 4.5-5.5 4%
37. Citrate: 6.4-7.5
34. FFP @ -65’C : 7 years 54. treatment for cutaneous T-cell lymphoma: Photopheresis
35. transport temperature for whole blood: 1-10’C 55. emergency cases (plasma): AB
36. pH of CPD anticoagulant in a 450 mL WB: 5.0-5.6 56. w/c of the ff are indications for platelet transfusion: plt
count <10,000/uL, <20,000/uL,
37. CP2D anticoagulant pH in 450 mL: 5.3-5.9
<200,000/uL
38. CPDA-1 anticoagulant in 450 mL: 5.0-6.0
57. Ab & binding site: Sensitization
39. ratio of CPD anticoagulant in 450 mL: 1.4:10
59. transfusion recipient for TA-GVHD EXCEPT: NOTA
40. contents of cooling plates: 1, 4-butanediol
60. w/c of the ff attacks the action or macrophages: IgG, IgG1,
41. platelet preparation not < : 20’C
1gG3, complement fragments
42. correct match for blood components: plt ≥ 5.5X10^10
61. hemolytic transfusion reaction EXCEPT: Hypertension
43. FFP should be transported: 1-10’C
62. presentation of anaphylactoid reaction: Anxiety
44. SAGM prevent hemolysis: Mannitol
63. African countries w/  risk of HIV-1: Nigeria, Cameroon
45. platelet agitation: facilitate O2 transfer, maintain pH
64. Hep B surface Ag: 1972
46. recombinant proteins used in transfusion medicine: VIII,
65. malaria, fatigue, anorexia, arthralgia, nausea, vomiting,
XIII, EPO, TPO (AOTA)
abdominal pain, fever, chagas disease:
47. centrifugation of batch or cycles: intermittent flow
INDEFINITE deferral
48.  Hgb about 2-3 g/dL or Hct 6-9% in pedia: 10-15 ml/kg
67. cause transfusion associated disease: Parvovirus B16
49. replacement fluid: 5% Albumin most widely used
68. Hep B: HBsAg; Hep C: Anti- HCV; HIV: Anti-HIV-1/2
50. pre-transfusion specimen for testing red cell transfusions except: WNV DNA
valid for: 72 hours
69. most frequently used for complex antibody: EITHER ficin
51. storage temperature for pretransfusion sample: 1-6’C or papain

52. pre-transfusion samples should be kept: 1-6’C for 7days 70. pathogen reduction plasma: AOTA (MB, Psoralen,
Riboflavin, Solvent detergent test)
53. treatment for hairy cell leukemia: IF-α
71. method to measure residual leukocyte: Nagaotte 86. Ab rarely cause HDFN except: Anti-K
hemocytometry, Flow cytometry
87. (+) for Donath-Landsteiner test: PCH
72. true of Apheresis: NOT Latin but Greek
88. associated w/ WAIHA EXCEPT: Ovarian carcinoma 89.
73. True regarding NAT: most common screening test: Rosette
74. 1st step in most DNA & RNA: Nucleic acid isolation test
75. adsorb Bg-like Abs from serum: Platelet concentrate 90. w/c of the following is true of CAIHA:
76. total elution w/ the use of pH: Wash Ab coated cell w/ 91. frequent autoantibody in cold: Anti-I
glycine @ pH 3; citric acid & digitonin
92. true of PCH except: Lab finding extravascular hemolysis
can also be used
93. SOP: Quality assurance
77. Rh discrepancies: Isolation of cell population, adsorption-
94. organizing of customer and suppliers: Quality management
elution using Rh serum
95. to detect, investigate, suspect transfusion reaction:
78. 1
Hemovigilance
st to perform plasmapheresis: J.L. Fahey
96. volume of AHG dispensed: Monthly
79. Mutiple Abs, warm & cold, potent cold antibody binding
97. TTD marker testing: Each test run
complement in AG phase
98. agitator: Day of use
80. disadvantages of SPRCA: Need for specialized equipment,
Increased sensitivity 81. adverse 99. NH4Cl: Irritant
effect of apheresis: Citrate toxicity, vasovagal reaction, air 100. CuSO4: Irritant
embolus, lymphocyte loss
82. action of irradiation on T-cells: Eliminates mitogenic
capacity of transfused T-cells, eliminates
blastosis 83. gene therapy in human: Somatic 84. destroys M
& N Ags: Papain & chymotrypsin 85.
affect severity of HDFN:
3. blood typing
result (anti A, B & H: 0): Bombay
4. more pronounced hangover: A
5. criminality: B
6. good teeth: O
7. Rh (-) mutations: European, African, Asian
8. Rh first discovered: patient suffered from ACUTE HTR
9. conversion of Rhorh'hr": DCe
10. postulated that D antigen has subparts: Wiener
11. possible antigens of P1 phenotype: 3 of these except P2K
12. agglutinin reported in Melanesians: IT
13. parasite receptor glycophorin A for P. falciparum: Duffy
14. Abs asso w/ AIHA: 3 of these except JK2
15. anti-Lu is found in patient w/ what condition: SLE
16. Lutheran Ags location: type 1 transmembrane protein
17. Dombrock location: EITHER (art 4 & adptransferase 4)
18. all destroy Ok except: AOTA (OK is resistant)
19. all are correct match for transfusion medicine except:
NOTA
20. each of the ff stains light bands not transcriptionally active:
Achromatin
21. all of the following are DNA repair system EXCEPT: NOTA
22. term used for the original form of DNA: Wild type
23. a pilot donated at 8am what time is allowed to pilot an
aircraft: Not < 12 hours
24. Cielo, hemoglobin is 125 g/L is she eligible to donate: No,
low hemoglobin
25. A 30 year old taking food supplements: Yes, no deferral for
food supplements
26. Greg, glutathione drip: No, defer for 12 months after last
dose
27. Esther, on-off fever, swollen lymph nodes: No, suspect of
HIV
28. A 21 year old severe acne, tetracycline as prophylaxis:
Yes, can donate anytime 29. James
Christopher Harrison, rare antibody use for anti-D reagent: up
to 6 standard WB donation per year for
males
30. Alice, a breastfeeding mom wants to donate blood: 3
months after weaning
31. polio sabin vaccine: 2 weeks
32. who can donate [DOH]: 35 year old w/ glaucoma treated
w/ eyedrops
33. 450 ml collection, can be stored after thawing 1-6’C: 15
days
34. rejuvenation: Incubate 50 ml RBC for 1 hour at 37’C
35. type of freezer high glycerol: Mechanical
36. sodium phosphate: Braxton Hicks
37. Citrate dextrose: Rous & Turner
38. Cytoplasmic calcium protein: Calmodulin
39. blood preservation goal: 1
st statement correct, 2nd statement incorrect (in vitro in vivo)
40. Benefits of additive sol’n: B
41. perfluorochemical advantages: biological inertness, lack of
immunogenicity, easily prepared
42. cryopreservation of platelets: DMSO
43. dose of radiation is 25 gy, what dose is applied to any
other point of the container: 15 gy
44. what anticoagulant used for neonatal transfusion: CPDA-1
45. glycerol for freezing RBC: 100 ml
46. thawing temperature for PF24: 30-37’C
47.cryopoorplasma contains all of the following EXCEPT:
Factor V & fibrinogen
48. produced by recombinant technology: Activated factor VII
49. prepared from salvaged plasma: EITHER (NSA or PPF)
50. factor IX concentrate prepared by : Monoclonal antibody
51. stabilizers for factor VIII: C EXCEPT glycine
52. WB collected at 10 AM, wants platelets concentrate: Within
4 hours of collection
53. severe chronic anemia: PRBC
54. anaphylactic reactions: Washed PRBC
55. serious bacterial infection: Granulocyte pheresis
56. anemia & Wiskott Aldrich syndrome? Irradiated PRBC
57.  1’C, chills , tachycardia: FNHTR (2’C: sepsis)
58. respiratory distress: TRALI
59. interpet the diagnosis… DAT(+):AIHA
60. GVHD: C EXCEPT BM examination hypocellular
61. post-transfusion purpura platelet ct. returning after: 2
weeks
62. unit of RBC, mg of Iron: 250 mg
63. incubation period for HCV: 2-26 weeks
64. 1
st retrovirus discovered: HTLV-1
65. 1’ amplifying host for WNV: birds
66. high risk CMV: EITHER
67. fifth disease: Parvovirus B19
68. isolate from platelets: EITHER
69. true of clinically significant antibodies: A
70. group O cells as reagent: anti-A & anti-B will not interfere
71. all of the ff exhibit dosage EXCEPT: D
72. coombs check cell do not aggluntinate: Repeated from the
beginning
73. advantages of gel technique: 2 OF THESE (sensitive & 93. set of activities CONTINUOUSLY processed goals:
washing omitted) Process control
74. interpret Ab screening test (+Sc 2): Probably IgG antibody 94. strategy identifying opportunities: Values stream
assesment
75. cold autoantibody: Anti I
95. QC hemoglobinometers: Day of use
76. can agglutinate anti-I: Breast milk
96. hazard.. skin contact: Toxic
77. human RBCs
97. dispensed AHG: Monthly
78. gentle heat method for elution: 45’C
98. pH meters: Day of use
79. pH of glycine: 3
99. delivery of Cesium 137: Yearly
80. not significant antibodies but can mask: AOTA
100. blood irradiators: Yearly
81. reagents stripping to allow phenotyping: 2 OF THESE
82. adding antigen allows to bind: Adsorption
83. chemicals used for phenotyping & autoadsorbing
procedures: 3 OF THESE
84. antibody pairs separated: Kidd & Duffy
85. most common cause of AIHA w/ DAT(-): A
86. acute form: M. pneumoniae
87. cold agglutinin disease: Complement
88. pathologic cold agglutinins reactive at: 37’C
89. newborns severe anemia: 7 g/dL
90. Other indication for RHIg: 3 OF THESE
91. Intrauterine transfusion: every 2-4 weeks
92. relative concentration of all antibodies crossing placenta:
Ab titers
1. most important blood group in both transfusion & & P. falciparum: 1 ONLY
transplantation Medicine: ABO
17. soluble protein of SdA: Tamm-Horsfall
2. first to perform forward & reverse typing: Karl Landsteiner
18. Augustine resistant to enzymes EXCEPT: NOTA
3. dye for yellow anti-B: Acriflavine
19. codominant : ALL
4. rule when performing ABO blood typing: clear solutions first
20. Hardy-Weinberg: ALL EXCEPT III. must ‘NOT’ occur in
before RBC
parents or children
5. anti-H: 0: Bombay
Louise Aluyen, RMT Mikee Ballo, RMT | 2
6. discovery of Rh: HTR in an obstetrical patient
21. resting stage: Interphase
7. 5 common Rh antigens produced by 3 closely-linked genes:
22. one strand of duplex DNA is converted to RNA:
Fisher-Race
Transcription
8. false (-) Rh typing: Saline suspended cells
23. chronic alcoholism: permanent deferral
9. false (+) Rh typing: Bacterial contact
24. ibuprofen allergy (drug allergy): 1 year deferral
10. most potent Rh Ag & highest occurring gene: D
25. vitamin C: Yes, because there is no deferral for vitamins
11. Lewis antigen: (1) agglutinated by saline (2) Lewis B is
26. MSM: Indefinite deferral
common: 1 ONLY
27. peptic ulcer, symptom free: 3 months deferral
12. P1: (1) rapidly expressed at birth (2) deteriorates on
storage: BOTH 28. from Ireland (2015): 6 month deferral ( vCFJD)
13. I & i : (1) high prevalent Ags (2) expressed in reciprocal 29. Glutathione capsule: Yes, can donate anytime
relationship: BOTH
30. fungal medications on antecubital fossa: 1 month deferral
14. M & N : (1) found on Glycophorin A (2) antithetical: BOTH
31. DOH 4 weeks deferral (1) H1N1 (2) BCG (3) Japanese
15. anti-K: (1) usually IgG (2) usually made in response to encephalitis (4) MMR : 1 & 2
AHG: BOTH
ONLY
16. Duffy: (1) fiirst described from hemophilia patient (2) null
phenotype resistant to P. vivax 32. DOH, Hepa B Immunoglobulin vaccine: 1 year deferral
33. mm of Adenine in CPDA-1: 0.25
34. chelates calcium: AOTA
35. max storage of low glycerol: -120 C
36. all are advantage of RBC freezing EXCEPT: NONE
37. RBC aliquot can  hemoglobin by: 2 g/dl
38. frozen RBCs shelf life: 10 years
39. deglycerolized RBCs: 24 hours
40. pooled platelet: 4 hours
41. FFP stored @ <-65 C: 7 years
42. granulocytes: 1 day
43. to prepare RBC & PRP according to AABB: 2000g x 3 mins
above
44. RBC w/o additive solution: 80%
45. PRP: soft spin only
46. Bacterial contamination introduced to plates by: Both
47. RDP: 5.5 x 10^10
48. SDP: 3 x 10^11
49. Leukoreduced WB: 5 x 10^6
50. Leukoreduced RDP: 8 .3 x 10^5
51. normal factor V, slightly reduced Factor VIII: PF24
52. PF24 & FFP thawed: 30C & 37C
53. major indication of washed RBCs: AOTA
54. major indication of pooled cryoprecipitate: Factor VIII
deficiency, Hypofibrinogenemia
55. special consideration for PF24 EXCEPT: Must be ABO
compatible
56. less platelet increment & survival: AOTA
57. functions of MT: 3 of these EXCEPT evaluate results
58. post transfusion sample procedures: ALL EXCEPT IAT
59. AABB requires EXCEPT: IAT
60. lab test findings confirm hemolysis: AOTA
61. serologic evidence of IHTR: (1) positive DAT IgG (2)
positive elution: BOTH are correct
62. primary pulmonary cause of TRALI: 2 OF THESE except
sepsis and shock
63. surrogate marker for non-A non-B hepa: Both ALT & Anti-
HCV
64. required test for Hepa B: 3 OF THESE except HbcAg
65. positive HIV in bloodbag: Duplicate → send to RITM
66. Fifth disease: Parvovirus B19
67. confirmation for syphilis: 3 OF THESE except RPR
68. transfusion associated parasites: 3 OF THESE except
Borrelia
69. HDN in first pregnancy, not predicted by titer: ABO HDN
70. rare to cause HDFN: ONE OF THESE (Anti-K)
71. ABO HDN causes anemia at birth while Rh does not: 89. Lui freeze thaw method: -18’C
BOTH ARE FALSE
90. pH of 3: pH method
72. week of gestation for clinical dx of fetal anemia: 16th
91. compatible: NOTA
-20th week
92. type AB negative emergency w/ no AB: A- first choice
73. broad category immune hemolytic transfusion reaction:
93. detect errors: Inspection program
AOTA
94. applicable requirements: BOTH
74. directed against individual’s own RBC: Autoantibodies or
autoagglutinins 96. fundamental of management: AOTA
75. thermal range of pathologic cold antibody: >30’C 76. PCH 97. potential errors: AOTA
in viral illnesses: AOTA
98. set of planned worked as expected:
77. transfusion testing method of IAT: Tube method
99. set of internal… to transform: Process control
78. RBC reagent antibody screen: Group O
100. DMAIC: Define Measure Analyze Improve Control
79. all are negative except for SC2 positive in AHG
polyspecific (table): 3 OF THESE
80. positive only in Immedate spin phase (table): 2 OF THESE
81. positive in all phases (table): 2 OF THESE
82. true of Ag-identification panel: AOTA
83. adsorb Bg-like substance: Human platelet concentrate
84. simple removal of autoantibodies: Adsorb own RBCs
85. phenotype unknown, antigen-type not an option:
Differential adsorption
86. most antibodies react at pH: 6.8-7.2
87. simplest elution method: Temperature-dependent
88. temp elution for heat: 56’C
1. only blood group with antibody in serum that are absent in 23. common deferral: Hgb
RBC: ABO
24. Soriatane: 3 years
2. ABO antigens: Pollen, bacteria
25. Plavix: 14 days
3. agglutinates: Lectins
26. skin graft: 12 months
4. Nature of Rh antigens: Protein
27. for platelet: Diversion pouch
5. A1
28. ACD-B pH: 4.5-5.5
6. positive for elution adsorption: A (M Y El)
29. ACD-B anticoagulant ratio: 4:10
7. Asians: DCe
30. syphilis:1950s
8. Rh antibodies: 37’C
31. CPD: 5.0-6.0
9. H gene: 19 long arm
32. CP2D: 5.3-5.9
10. Caucasians: c
33. minimum hematocrit to anticoagulant: 33%
11. 2nd discovered after ABO: MNS
34. neonatal pediatric:<7 to 10 days (CPD)
12. MNS antibodies: MN-dosage, IgM, commonly encountered
35. lessen hemolysis: Additive solution
IgM
36. low blood volume: EXCEPT platelet, FFP, cryoprecipitate
13. PPIpK: AOTA
ONLY RBC
14. autoanti-P: PCH, biphasic, Donath-Landsteiner
37. PRP: Soft spin only
17. Raph blood group: Mer2
38. plasma platelet suspended: 40-70 ml
18. Null knops: Hegelson
39. from buffy coat: 41 ml
19. transfusion med: Molecular gen & Dx, biotechnology
40. FFP prepare: within 8 hrs
20. DNA 28- 45 kb long: Cosmids; Plasmid: vector
41. -65C: 7 years
21. gene therapy allowed in humans: Somatic
42. viable lymphocytes in thawed FFP from soft spin: 0.04 to
22. dark band: Euchromatin 3.6 x 10^6
43. albumin/Ig prepared from: Recovered plasma 68. Epstein Barr virus
44. inactivate microbial agent EXCEPT: BCB 69. pH elution: pH 3 w/ citric acid
45. added to PVC to prevent rigidity of blood container: DEHP 70. organic solvent EXCEPT: Benzene
46. irradiated EXCEPT: Cryoprecipitate 71. DAT: Monospecific
48. transport for WB: 1-10’C 72. False + DAT: Monospecific
49. replacement fluid: 5% Albumin, hyperoncotic 73. advantage of gel and solid tech: 3 OF THESE
50. thawed plasma shelf life: 5 days 74. remove Ab in serum: Adsorption
51. cold insoluble portion: Cryoprecipitate 75. RBC phenotyping & autoadsorption: ZZAP, EGA,
Chloroquine diphosphate
52. plt in RD: 5.5 x 10^10
76. enzyme treatment separated: Anti-Jka & Anti-Fya
53. bar code: AOTA
77. Rh discrepancies EXCEPT secretor studies
54. apheresis: AOTA
78. polyspecific AHG: AOTA
55. FDA apheresis: ALL correct EXCEPT male & female
weight & height 79. Lewis EXCEPT Pepsin
56. adequate granulocytes for infants/children: 10-15 ml/kg 81. Sensitization
57-61 : Read TRALI, TACO, TAS , FNHTR, TA GVHD) 82. IgM vs IgG: B2 mercaptoethanol, dithiothreitol
62. massive transfusion: 50% in 3 hrs 83. ficin: Fig
63. spongiform encephalitis: variant CJD 84. HDFN severity: AOTA
64. HIV-1: Cameroon, Nigeria 86. Rarely cause HDFN EXCEPT: anti-K
65. adverse effect: AOTA 87. HDFN: 18-20 weeks
66. macrophages: complement frag, Quantity IgG or Com, # 88. anemia in fetus: Colon Doppler ultrasonography
helper T cells
89. unconjugated bilirubin: 460-490 nm
67. HTLV: first retrovirus
90. not involved in HDN: IgD
91. kernicterus: 18-20 mg/dL 12. Xg
92. screening fetal blood: Rosette test 13. Scianna
93. Biovigilance 14. Dombrock
94. waterbath: Day of use 15. Colton
95. coppersulfate: Day of use 16. Landsteiner-Weiner
96. shipping container: 2x a year 17. Chido/Rodgers
97. hemoglobinometer: Day of use 18. H
98. blood pressure cuff: 2x a year 19. Kx
----------------------------------------------------------------------------------- 20. Gerbich
---------------------------------------
21. Cromer
ISBT #
22. Knops
1. ABO
23. Indian
2. MNS
24. Ok
3. P1PK
25. Raph
4. Rh
26. John Milton Hagen
5. Lutheran
27. I
6. Kell
28. Globoside
7. Lewis
29. Gill
8. Duffy
30. Rh-associated glycoprotein
9. Kidd
10. Diego
11. Yt
HLA Disease Associations
A3: hemochromatosis
B13 & 17: psoriasis
B27: ankylosing spondylitis & Reiter’s
B38: phemphigus vulgaris
B51: Behcet
DR2: narcolepsy
DR3: dermatitis
DR3: Hashimoto
DR4: rheumatoid arthritis
-----------------------------------------------------------------------------------
---------------------------------------
Reactivity
IgM IgG
M, N, P, I, Lua Rh, S, s, U, Kell, Duffy, Kidd, Lub
CPD
dextrose: 1610 (CPD), 3220 (CP2D), 2010 (CPDA-1)
Na citrate: 1660 citric acid: 206 Na phosphate: 140
adenine: 17.3 (CPDA-1)