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Total number of originals: 47 (Including reproduction specification)

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NOTES:

1. SHEETS 2-47 TO BE PRINTED BACK TO BACK.

2. ALL ORIGINAL PRINTING MASTERS AND ARTWORK MUST BE RETURNED TO DADE
MICROSCAN INC., ATTN.: DOCUMENT SERVICES.
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IMP REV ECN NO. APP DATE APPROVALS DATE

DLH A 00-10302 M Harding 04/08/02 R&D G Galvan 04/08/02 Dade MicroScan Inc.
ME/PE C Griner 04/05/02 West Sacramento, CA 95691 USA
QA P Burrell 04/08/02
autoSCAN-4 LABPRO
MAT J McClure 04/08/02
DIAGNOSTICS MANUAL
SIZE DRAWING NO REV
CKD (CURRENT REV) R Guzman 04/05/02 A 9020-6627 A
REQ (CURRENT REV) B Bleakly 04/05/02 SCALE N/A Sheet 1 of 47
 USER MANUAL

autoSCAN -4
DIAGNOSTICS
LABPRO

9020-6627A For use by Dade Behring MicroScan Trained Personnel only

1 Introduction .............................................................................................................................................. 6

Figure 1.1 autoSCAN -4 Diagnostics...................................................................................... 6
1.1 Status Bar ......................................................................................................................................... 6
2 Diagnostics Password .............................................................................................................................. 7
Figure 2.1 Diagnostics Password Dialog Box ......................................................................... 7
2.1 Password .......................................................................................................................................... 7
2.2 Instrument Serial Number................................................................................................................ 7
2.3 Instrument Serial Port ...................................................................................................................... 7
2.4 Okay Button ..................................................................................................................................... 7
2.5 Cancel Button................................................................................................................................... 7
3 Data Tab ................................................................................................................................................... 8
3.1 Voltage/Gain Tab............................................................................................................................. 8
Figure 3.1 Data – Voltage/Gain Tab........................................................................................ 8
3.1.1 Select Test Point ...................................................................................................................... 8
3.1.1.1 +10 Volt Standard (TP 9)..................................................................................................... 8
3.1.1.2 -15 Volts (TP 12) ................................................................................................................. 9
3.1.1.3 Analog Ground (TP 2) ......................................................................................................... 9
3.1.1.4 +15 Volts (TP 10) ................................................................................................................ 9
3.1.1.5 +1 Volt Standard (TP 5)....................................................................................................... 9
3.1.1.6 DAC Offset (TP 11)............................................................................................................. 9
3.1.1.7 2/3 Log Amp Output (TP 14)............................................................................................... 9
Table 3.1 2/3 Log Amp Output Signals (+/- 1%) .................................................................... 9
3.1.1.8 Log Amp Output (TP 14)..................................................................................................... 9
Table 3.2 Log Amp Output (+/-1%) ...................................................................................... 10
3.1.1.9 +1 Volt at Photo Board ...................................................................................................... 10
3.1.1.10 Signal Input Photo Board (TP 1) ................................................................................... 10
3.1.1.11 Reference Input Photo Board (TP 3) ............................................................................. 10
3.1.1.12 +5 Volts (TP 8) .............................................................................................................. 10
3.1.1.13 2/3 DAC Output (TP 11)................................................................................................ 10
Table 3.3, 2/3 DAC Output (+/-10%) .................................................................................... 10
3.1.1.14 2/3 Reference in at Log Amp (TP 13) ........................................................................... 10
Table 3.4, 2/3 Reference Input to Log Amp (+/-5%) ............................................................ 11
3.1.1.15 DAC Output (TP 11)...................................................................................................... 11
Table 3.5, DAC Output (+/-10%) .......................................................................................... 11
3.1.1.16 Reference in at Log Amp (TP 13) ................................................................................. 11
Table 3.6 Reference In At Log Amp (+/-5%)........................................................................ 11
3.1.2 Measured Voltage .................................................................................................................. 11
3.1.3 Select Measured Voltage ....................................................................................................... 11
3.1.3.1 Adjust Final Gain ............................................................................................................... 11
Figure 3.2 Adjust Final Gain ................................................................................................. 12
3.1.3.2 Selected Test Point............................................................................................................. 12
Figure 3.3 Selected Test Point ............................................................................................... 13
3.1.4 Controls.................................................................................................................................. 13
3.1.4.1 Signal Input ........................................................................................................................ 13
Table 3.7 Signal Parameters for A/D - Photo Board Checks ................................................ 14
3.1.4.2 Reference Input .................................................................................................................. 14
Table 3.8 Reference Parameters for A/D – Photo Board Checks.......................................... 14
3.1.4.3 DAC ................................................................................................................................... 14
Table 3.9 DAC Test (+/- 10%) .............................................................................................. 14
3.1.5 Print Report button................................................................................................................. 14
3.1.6 Exit button.............................................................................................................................. 15
3.2 Calibration Tab .............................................................................................................................. 15
Figure 3.4 Data – Calibration Tab ......................................................................................... 15
Table 3.10 Air Calibration Filter Table ................................................................................. 15
3.2.1 Display Box............................................................................................................................ 16

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 3.2.1.1 Data .................................................................................................................................... 16
3.2.1.2 DAC ................................................................................................................................... 16
3.2.2 Calibrate Button ..................................................................................................................... 16
3.2.3 Filter Arrows .......................................................................................................................... 16
3.2.4 Print Button ............................................................................................................................ 16
3.2.5 Exit Button ............................................................................................................................. 16
3.3 Repeatability Tab ........................................................................................................................... 17
Figure 3.5 Data – Repeatability Tab ...................................................................................... 17
Table 3.11, Panel Filter Table ................................................................................................ 17
3.3.1 Display Group ........................................................................................................................ 17
3.3.1.1 Data .................................................................................................................................... 18
3.3.1.2 Average .............................................................................................................................. 18
3.3.1.3 Difference........................................................................................................................... 18
3.3.1.4 Min ..................................................................................................................................... 18
3.3.1.5 Max..................................................................................................................................... 18
3.3.2 Read Number Edit Scroll Box................................................................................................ 18
3.3.3 Delay[sec]............................................................................................................................... 18
3.3.4 Read Panel Button.................................................................................................................. 18
3.3.5 Filter Buttons.......................................................................................................................... 18
3.3.6 Read Number Buttons ............................................................................................................ 18
3.3.7 Print Button ............................................................................................................................ 19
3.3.8 Exit Button ............................................................................................................................. 19
4 QC Test .................................................................................................................................................. 19
4.1 QC Tests [Self Tests] ..................................................................................................................... 19
Figure 4.1 Data – QC Test Tab .............................................................................................. 19
4.1.1 QC Test Button ...................................................................................................................... 19
4.1.2 PROM..................................................................................................................................... 20
4.1.3 RAM....................................................................................................................................... 20
4.1.4 Power Supply Voltages .......................................................................................................... 20
4.1.5 Light Source ........................................................................................................................... 20
4.1.6 A/D Photo Board.................................................................................................................... 20
4.1.7 Color Wheel ........................................................................................................................... 20
4.1.8 Drawer Motion ....................................................................................................................... 20
4.1.9 Print Button ............................................................................................................................ 20
4.1.10 Exit Button ............................................................................................................................. 20
5 Control.................................................................................................................................................... 21
Figure 5.1 Control Tab........................................................................................................... 21
5.1 Colorwheel ..................................................................................................................................... 21
Figure 5.2, Control – Filter Motion Box................................................................................ 21
5.1.1 Select Specific Filter .............................................................................................................. 21
5.1.2 Home ...................................................................................................................................... 22
Table 5.1, Colorwheel Filter Table ........................................................................................ 22
5.1.3 Continuous Filter Motion....................................................................................................... 22
5.1.4 Total Cycles ........................................................................................................................... 22
5.1.5 Seconds Between Movements ............................................................................................... 22
5.2 Drawer ............................................................................................................................................ 23
Figure 5.3, Control – Drawer Motion Box............................................................................. 23
5.2.1 Select Specific Position.......................................................................................................... 23
5.2.1.1 Open Radio Button............................................................................................................. 23
5.2.1.2 Closed Radio Button .......................................................................................................... 23
5.2.2 Continuous Motion................................................................................................................. 23
5.2.3 Total Cycles ........................................................................................................................... 23
5.2.4 Seconds Between Movements ............................................................................................... 23
5.2.5 Position Indicator ................................................................................................................... 24
5.2.6 Closed Opto Indicator ............................................................................................................ 24
5.2.7 Open Opto Indicator............................................................................................................... 24

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6 Alignment............................................................................................................................................... 24
6.1 Power Supply ................................................................................................................................. 24
Figure 6.1, Alignment – Power Supply Tab .......................................................................... 24
6.1.1 Step 1...................................................................................................................................... 25
Figure 6.2, Alignment – Power Supply – Step 1 ................................................................... 25
6.1.2 Step 2...................................................................................................................................... 26
Figure 6.3, Alignment – Power Supply – Step 2 ................................................................... 26
6.1.3 Step 3...................................................................................................................................... 26
Figure 6.4, Alignment – Power Supply – Step 3 ................................................................... 26
6.1.4 Step 4...................................................................................................................................... 27
Figure 6.5, Alignment – Power Supply – Step 4 ................................................................... 27
6.2 Reference Fiber .............................................................................................................................. 27
Figure 6.6, Alignment – Reference Fiber Tab ....................................................................... 28
6.2.1 Step 1...................................................................................................................................... 28
Figure 6.7, Alignment – Reference Fiber – Step 1 ................................................................ 28
6.2.2 Step 2...................................................................................................................................... 28
Figure 6.8, Alignment – Reference Fiber – Step 2 ................................................................ 28
6.2.3 Step 3...................................................................................................................................... 29
Figure 6.9, Alignment – Reference Fiber – Step 3 ................................................................ 29
6.3 A/D Board ...................................................................................................................................... 29
Figure 6.10, Alignment – A/D Board Tab ............................................................................. 30
6.3.1 Step 1...................................................................................................................................... 30
Figure 6.11, Alignment – A/D Board – Step 1 ...................................................................... 30
6.3.2 Step 2...................................................................................................................................... 31
Figure 6.12, Alignment – A/D Board – Step 2 ...................................................................... 31
6.3.3 Step 3...................................................................................................................................... 31
Figure 6.13, Alignment – A/D Board – Step 3 ...................................................................... 31
6.3.4 Step 4...................................................................................................................................... 32
Figure 6.14, Alignment – A/D Board – Step 4 ...................................................................... 32
6.3.5 Step 5...................................................................................................................................... 33
Figure 6.15, Alignment – A/D Board – Step 5 ...................................................................... 33
6.4 Offset Test Panel............................................................................................................................ 33
Figure 6.16, Alignment – Offset Test Panel Tab................................................................... 34
6.4.1 Step 1...................................................................................................................................... 34
Figure 6.17, Alignment – Offset Test Panel – Step 1 ............................................................ 34
6.4.2 Step 2...................................................................................................................................... 35
Figure 6.18, Alignment – Offset Test Panel – Step 2 ............................................................ 35
Figure 6.19, Alignment – Offset Test Panel – Step 2B ......................................................... 35
6.4.3 Step 3...................................................................................................................................... 36
Figure 6.20, Alignment – Offset Test Panel – Step 3 ............................................................ 36
Figure 6.21, Alignment – Offset Test Panel .......................................................................... 37
Figure 6.22, Alignment – Offset Test Panel – Step 3C ......................................................... 37
7 Test......................................................................................................................................................... 38
Figure 7.1, Test Tab ............................................................................................................... 38
7.1 PROM Checksum .......................................................................................................................... 39
Figure 7.2, Test – PROM Checksum ..................................................................................... 39
7.2 RAM Memory................................................................................................................................ 40
Figure 7.3, Test – RAM Memory .......................................................................................... 40
7.3 Lamp .............................................................................................................................................. 41
Figure 7.4, Test – Lamp ......................................................................................................... 41
7.4 Drawer............................................................................................................................................ 42
Figure 7.5, Test – Drawer ...................................................................................................... 42
7.4.1 Cycle ? of List Box ................................................................................................................ 42
7.4.2 Clear Tests Button.................................................................................................................. 42
7.4.3 Run Button ............................................................................................................................. 42
7.4.4 Cancel Button......................................................................................................................... 42

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 7.4.5 Print Results Button ............................................................................................................... 42
7.4.6 Results Box ............................................................................................................................ 43
8 File.......................................................................................................................................................... 43
Figure 8.1, File Drop Down Box ........................................................................................... 43
8.1 Log ................................................................................................................................................. 44
Figure 8.2, File – Log............................................................................................................. 44
8.2 Parameters ...................................................................................................................................... 45
Figure 8.3, File – Parameters ................................................................................................. 45
8.3 Password......................................................................................................................................... 45
Figure 8.4, File – Password.................................................................................................... 45
8.4 Exit ................................................................................................................................................. 45

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1. Introduction
The autoSCAN-4 Diagnostics is a Windows-NT program used to analyze the autoSCAN-4 Instrument.
All features are grouped by their common functionality. The Data Tab displays instrument voltages, A/D
board gain, allows the user to read panels, and calibrates the autoSCAN-4 Instrument. The Control Tab
allows the user to position the drawer and colorwheel. The Alignment Tab guides the user through the
Power Supply, Reference Fiber, A/D Board, and Offset Test Panel alignments. The Test Tab allows the
user to execute autoSCAN-4 internal diagnostics and test the drawer movement.


Figure 1.1 autoSCAN -4 Diagnostics

1.1 Status Bar

The Status Bar is located at the bottom of the screen and is divided into three sections. Section
one displays status messages for the application. Section two specifies the serial port in use (e.g.,
Comm 1) or if the application is in Demo mode. Section three displays the autoSCAN-4 serial
number as entered by the user.

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2 Diagnostics Password
The autoSCAN-4 program is password protected to prevent untrained individuals from accessing the
program.

Figure 2.1 Diagnostics Password Dialog Box

When the autoSCAN-4 Diagnostics program is executed, the Diagnostics Password dialog box (Figure
2.1) will be displayed. The user must enter the autoSCAN-4 serial number, serial port the instrument is
connected to, and a valid password before the autoSCAN-4 Diagnostics Main Menu is displayed.

2.1 Password
The user must enter a pre-determined password. If the password field is left blank the program
will be in DEMO mode. The user will have access to all autoSCAN-4 Diagnostics screens but
the software will not communicate with the autoSCAN-4 Instrument. As the user enters a
password, asterisks (*) will be displayed to prevent others from learning the password. The
password is not case sensitive.

2.2 Instrument Serial Number

The Serial Number field identifies the serial number of the autoSCAN-4 Instrument. The user
must manually enter the serial number each time the program is executed. The serial number is
limited to a five-digit number.

2.3 Instrument Serial Port

The Serial Port field identifies the serial port the autoSCAN-4 Instrument is connected to on the
computer. Valid serial ports are 1 through 9.

2.4 Okay Button

When the Okay button is selected, the program will verify a valid password has been entered, try
to open the serial port to ensure it is available, and attempt to establish communication with the
autoSCAN-4 Instrument. If any of these items fail, then the user will not be allowed to continue.
If the user presses the Okay button after entering just the instrument serial number the software
runs DEMO mode.

2.5 Cancel Button

Selecting the Cancel button will terminate the autoSCAN-4 LabPro Diagnostics.

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3 Data Tab
The Data Tab contains the autoSCAN-4 Voltages/Gain, Calibration, Repeatability and QC Test
information.

3.1 Voltage/Gain Tab

Figure 3.1 Data – Voltage/Gain Tab

The Voltage/Gain Tab (Figure 3.1) allows the user to select and monitor various autoSCAN-4 Voltages.

3.1.1 Select Test Point

3.1.1.1 +10 Volt Standard (TP 9)

The analog to digital converter generates a 10 VDC signal. The acceptable limits for this
voltage are 9.85 VDC to 10.15 VDC. If this test fails, check the power supply voltage.
Also verify that the interconnection to the CPU board is OK. If the test still fails, the A/D
is more than likely defective.

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 3.1.1.2 -15 Volts (TP 12)
-15 VDC is generated on the Power Control Board and applied to the A/D board. The
acceptable limits for this voltage are from -14 VDC to -16 VDC.

3.1.1.3 Analog Ground (TP 2)

This signal level should be 0 Volts +/-10 mV. Failure in this test could indicate a faulty
A/D board. Analog ground is the required reference point for test points 1, 3, 5, 7, 9, 10,
11, 12, 13, and 14.

3.1.1.4 +15 Volts (TP 10)

+15 VDC is generated on the Power Control Board and applied to the A/D board. The
acceptable limits for this voltage are from +14 VDC to +16 VDC. If the +15 volt needs to
be adjusted, use the Alignment Power Supply tab. If the voltage is not adjustable check
the power supply voltages, interconnection between the power board and A/D board,
control board, power board, and A/D board.

3.1.1.5 +1 Volt Standard (TP 5)

The A/D board also generates a 1 volt reference. This particular voltage level should be 1
volt +/-20 mV. If the voltage is out of range, check the power supply voltages. Verify
that the interconnection to the CPU board is OK. If the voltage still fails, the A/D board is
more than likely defective.

3.1.1.6 DAC Offset (TP 11)

The DAC Offset is designed to check the dynamic range of the offset adjustment. The
dynamic range of the offset is from -203 mV to +203 mV. If the screen displays a voltage
greater than plus or minus 170 mV, a problem may exist on the A/D board.

3.1.1.7 2/3 Log Amp Output (TP 14)

To verify the 2/3 Log Amp Output signal set the parameters as indicated in Table 3.1. An
error may indicate a defective A/D board. The DAC gain is set at 255 to achieve these
values.
SELECTED SIGNAL INPUT
SEL. REF. INPUT 0.010 0.100 1.000
0.100 0V +6.66 V N/A
0.500 -4.65 V +2.01 V N/A
1.000 -6.66 V 0V N/A
Table 3.1 2/3 Log Amp Output Signals (+/- 1%)

3.1.1.8 Log Amp Output (TP 14)

To verify the Log Amp Output signal set the parameters as indicated in Table 3.2. An
error may indicate a defective A/D board. The DAC gain is set at 255 to achieve these
values.

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 SELECTED SIGNAL INPUT
SEL. REF. INPUT 0.010 0.100 1.000
0.100 0V +10.0 V N/A
0.500 -6.98 V +3.01 V N/A
1.000 -10.0 V 0V N/A
Table 3.2 Log Amp Output (+/-1%)

3.1.1.9 +1 Volt at Photo Board

Selecting this option checks for +1 volts on the photo board. It should be 1 volt +/-20mV.
If this value is not correct, a problem could exist in the photo board or the photo board to
A/D board interconnect cable.

3.1.1.10 Signal Input Photo Board (TP 1)

The screen should indicate the signal value that was selected from Table 3.1 +/- 5% is
being received from the photo board. A problem may exist with the photo board if this
signal fails.

3.1.1.11 Reference Input Photo Board (TP 3)

The screen should indicate the reference value that was selected from Table 3.2 +/-5% is
being received from the photo board. A problem may exist with the photo board if this
signal fails.

3.1.1.12 +5 Volts (TP 8)

This verifies that the +5 volt is present on the A/D board. If the +5 volts needs to be
adjusted, use the Alignment Power Supply tab. If the voltage is not adjustable, check the
power supply voltages, interconnection between the power board and A/D board, control
board, power board, and A/D board.

3.1.1.13 2/3 DAC Output (TP 11)

The 2/3 DAC Output signal is used to verify the output of the DAC is providing correct
signals. Refer to Table 3.3 for the correct outputs.
SELECTED SIGNAL INPUT 2/3 DAC OUTPUT
0.010 V .667 V
0.100 V 6.67 V
Table 3.3, 2/3 DAC Output (+/-10%)

3.1.1.14 2/3 Reference in at Log Amp (TP 13)

The 2/3 Reference in at Log Amp signal is used to verify the reference signal is providing
the correct signal. Refer to Table 3.4 for the correct output.

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 Reference. Input 2/3 Reference Input to Log Amp
0.100 V .667 V
0.500 V 3.33 V
1.000 V 6.67 V
Table 3.4, 2/3 Reference Input to Log Amp (+/-5%)

3.1.1.15 DAC Output (TP 11)

The DAC Output signal verifies that the DAC is working correctly. Setting the DAC value
to 255 and the Signal Input per Table 3.5 will verify that the DAC output is working
correctly. An error may indicate a defective A/D board. The DAC gain is set at 255 to
achieve these values.
Signal Input DAC Output
0.010 V 1.0 V
0.100 V 10.0 V
1.000 V N/A
Table 3.5, DAC Output (+/-10%)

3.1.1.16 Reference in at Log Amp (TP 13)

The Reference in at Log Amp verifies that the reference signal is present at the input to the
log amp. Refer to Table 3.6.
Reference Input Reference In At Log Amp
0.100 V 1.0 V
0.500 V 5.0 V
1.000 V 10.0 V
Table 3.6 Reference In At Log Amp (+/-5%)

3.1.2 Measured Voltage

Displays the actual voltage reading of the voltage selected from the Selected Test Point box,
Selected Measured Voltage box, and Controls Box.

3.1.3 Select Measured Voltage

Allows the user to Adjust Final Gain or Select a test point from the Select Test Point box
and display the output in the Measured Voltage box.

3.1.3.1 Adjust Final Gain

Adjust Final Gain (Figure 3.2) monitors and displays the final gain output from the
Photo A/D board. The gain signal output level should be a approximately 10 volts.
During this process the Select Test Point Box items and Controls Box are grayed out and
inaccessible to the user.

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 Figure 3.2 Adjust Final Gain

3.1.3.2 Selected Test Point

When Selected Test Point is selected the user is able to select and read test points in the
Selected Test Point Box (Figure 3.3).

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 Figure 3.3 Selected Test Point

3.1.4 Controls

3.1.4.1 Signal Input

The Signal Input determines which input is selected from the photo diode multiplexer for
processing on the A/D board. 0.010, 0.100 and 1.000 volts are reference voltages to test
the functionality of the A/D board. The External Input allows an external signal to be
supplied to the system (0 to +10 volts). The Normal signal input allows the photo diode
signals to be processed with the output voltage determined by the DAC setting, colorwheel
filter selection, and panel media. Table 3.7 shows the Signal Inputs and expected reading
at TP1. The DAC must be set to 255 for Table 3.7 to be valid.

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 Signal Input Signal Input Photo Board (TP1)
0.010 volts -0.010 volts
0.100 volts -0.100 volts
1.000 volts -1.000 volts
External input The output reading will be the same as the
(Photo Board –Test Point pin 10) voltage applied at External input
(A/D Board –Test Point pin 1)
Normal signal input Actual photo diode reading

Table 3.7 Signal Parameters for A/D - Photo Board Checks

3.1.4.2 Reference Input

The Reference Input determines which reference signal is being selected to the A/D board.
0.100, 0.500 and 1.000 volts are reference voltages to test the functionality of the A/D
board. The External Input allows an external signal to be supplied to the system (0 to +10
volts). The Normal signal input allows the reference diodes to be processed with the
output voltage determined by the colorwheel filter. Table 3.8 shows the Reference Inputs
and expected reading at TP3. The DAC must be set to 255 for Table 3.8 to be valid.
Reference Input Voltages Reference Input Photo Board (TP3)
0.100 volts -0.100 volts
0.500 volts -0.500 volts
1.000 volts -1.000 volts
External input The output reading will be the same as the
(Photo Board -Test Point pin 9) voltage applied at External input
(A/D Board –Test Point pin 3)
Normal reference input Actual reference diode reading

Table 3.8 Reference Parameters for A/D – Photo Board Checks

3.1.4.3 DAC
The DAC gain is developed on the A/D Board for automatic gain control to keep all photo
diode current levels at approximately the same level. The allowable range is 0 to 255.
Table 3.9 displays DAC Output (TP11) voltages for selected Signal Inputs with a DAC
gain of 255. An error indicates a defective A/D board.
Signal Input DAC Output
0.010 V 1.0 V
0.100 V 10.0 V
1.000 V N/A
Table 3.9 DAC Test (+/- 10%)

3.1.5 Print Report button

The Print Report button is disabled when the Voltages/Gain folder is selected.

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 3.1.6 Exit button
Selecting the Exit button will terminate the current screen and return the user to the LabPro
autoSCAN-4 Diagnostics Main Menu.

3.2 Calibration Tab

Figure 3.4 Data – Calibration Tab

The Calibration Tab (Figure 3.4) allows the user to calibrate the autoSCAN-4 Instrument. The air
calibration Data values and DAC values are displayed for each filter and well. The DAC readings should
be less than 235 units for all filters. DAC readings of 235 units or greater indicates that it is too dark,
which may be due to a defective lamp, fiber, photodiode, or dirt in the optical pathway (e.g. shield). The
calibration tab sheet also displays the autoSCAN-4 calibration results.
Wavelength Color Tolerance
620 nm Red 3600 units +/- 600 units
560 nm Green 3600 units +/- 600 units
505 nm Blue-Green (Aqua) 3600 units +/- 600 units
470 nm Dark Purple 3600 units +/- 600 units
440 nm Blue 3600 units +/- 600 units
590 nm Yellow 3600 units +/- 600 units
590 nm/A Yellow 3100 units +/- 300 units
Table 3.10 Air Calibration Filter Table

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 The Air Calibration Filter Table (Table 3.10) listed above and on the Calibration Tab lists the
colorwheel filter specifications for an air calibration. The 590 nm/A filter is the 590 filter with the
aperture activated (pulled in).

3.2.1 Display Box

The display option allows the user to view the calibration Data or the DAC values associated
with each filter.

3.2.1.1 Data
Selecting the Data radio button will display the air calibration data values. The Data values
for each filter must be within the listed in the Table 3.10.

3.2.1.2 DAC
Selecting the DAC radio button will display the air calibration DAC values for each filter.
The DAC values for each filter should be less the 235.

3.2.2 Calibrate Button

The Calibrate button is used to calibrate the autoSCAN-4 Instrument. Ensure that the
drawer is empty prior to calibrating.

3.2.3 Filter Arrows

The arrows enable the user to step through and view the read data for each filter.

3.2.4 Print Button

Prints the Calibration Report.

3.2.5 Exit Button

Exits the Calibration Tab and returns the user to the LabPro autoSCAN-4 Diagnostics Main
Menu.

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 3.3 Repeatability Tab

Figure 3.5 Data – Repeatability Tab

The Repeatability Tab (Figure 3.5) allows the user to read a panel up to 50 times to test repeatability.
Data, Difference, Maximum, Average, and Minimum values can be viewed or printed on this screen.
Wavelength Color
620 nm Red
560 nm Green
505 nm Blue-Green (Aqua)
470 nm Dark Purple
440 nm Blue
590 nm Yellow
590 nm/A Yellow
Table 3.11, Panel Filter Table

The Panel Filter Table (Table 3.11) lists each filters wavelength and color. The 590 nm /A filter is the 590
filter with the aperture activated (pulled in).

3.3.1 Display Group

The Display Box (Figure 3.5) contains the Data, Difference, Max, Average, and Min
buttons.

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 3.3.1.1 Data
The Data option fills the panel display with the actual panel data values.

3.3.1.2 Average
The Average option fills the panel display with the average read values of all the panels
read.

3.3.1.3 Difference
The Difference option fills the panel display with the values of the difference between the
last two-selected panel readings.

3.3.1.4 Min
The Min is the abbreviation for minimum. The Min option fills the panel display with the
smallest read values of the panel or panels read.

3.3.1.5 Max
The Max is the abbreviation for maximum. The Max option fills the panel display with
the largest read values of the panel or panels read.

3.3.2 Read Number Edit Scroll Box

The number of times a panel is read is placed in the Panel Number Edit Scroll Box. The
user may go back and examine any of the panel reads by selecting a particular panel read
number. The Read Number is used when running panel repeatability. The number of times a
panel is to be read is placed in the far right box. The instrument will read the panel for the
indicated number of times and will stop reading once the number has been reached. The
user can review any of the past reads by selecting a read number with the Read Number
buttons.

3.3.3 Delay[sec]
The Delay[sec] allows the user to determine the amount of time (in seconds) the program
will wait between each panel read. This should normally be set to zero. Possible entries are
between 0 and 99 seconds.

3.3.4 Read Panel Button

The Read button executes the panel read function. The Data, Average, Difference, Min and
Max values are then loaded into the filter matrix and displayed when appropriate radio
button is selected.

3.3.5 Filter Buttons

The Filter buttons allows the user to select any of the color wheel filters.

3.3.6 Read Number Buttons

The Read Number buttons allow the user to select and view the Panel Data from any one of
the reads listed in the Read Number Edit Scroll box. Selecting the right arrow button
increments through the reads. Selecting the left arrow button decrements through the reads.

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 3.3.7 Print Button
The Print button allows the user to print the current read and repeatability data.

3.3.8 Exit Button

The Exit button exits the user out of the Repeatability Tab and returns the user to the Main
Menu.

4 QC Test
The QC Test Tab is used to tests the PROM, RAM, Power Supply Voltages, Light Source, A/D Photo
Board, Color Wheel and Drawer Motion.

4.1 QC Tests [Self Tests]

Figure 4.1 Data – QC Test Tab

The QC Test Tab (Figure 4.1) allows the user to test the PROM, RAM, Power Supply Voltages, Light
Source, A/D Photo Board, Color Wheel and Drawer Motion.

4.1.1 QC Test Button

The QC Test button executes the PROM, RAM, Power Supply Voltages, Light Source, A/D
Photo Board, Color Wheel and Drawer Motion tests. The Passed and Failed results are
displayed directly adjacent to the test name.

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 4.1.2 PROM
The PROM Checksum box enables the user to test the PROM Checksum. If the reader
program (which controls the internal working functions of the instrument) has been
corrupted, the PROM checksum test should indicate this as a failed component. The CPU
board should be replaced.

4.1.3 RAM
The RAM Memory box allows the user to test the RAM Memory. All panel data gathered
by the CPU is temporarily stored in RAM before being sent to the computer. A RAM
memory failure requires replacing the CPU board assembly.

4.1.4 Power Supply Voltages

The Power Supply Voltages box tests the +5 volt, and +/- 15 volt power supplies. A power
supply failure indicates a blown fuse on the Power Controller Board or replacement of the
Power Controller Board or one of the two autoSCAN-4 Power Supplies.

4.1.5 Light Source

The Light Source tests will give an indication of whether the lamp is operating or burned
out. The color wheel should advance to a designated location and the user should observe
color from the front panel scanning indicator. A failure in this test indicates a defective
lamp and/or 12.3-volt power supply failure.

4.1.6 A/D Photo Board

The A/D Photo Board test the A/D board and the Photo Board to ensure the analog signals
generated by the photo board and the reference voltages are amplified in a uniform manner
on the A/D board.

4.1.7 Color Wheel

The Color wheel test checks the color wheel movement and positioning. This tests the opto-
interrupter feedback signal to the CPU when a filter is placed in the correct light path. The
CPU then stops the color wheel motor. A failure in this test could indicate a defective or
misalign opto-interrupter, motor drive circuitry or mechanical assembly.

4.1.8 Drawer Motion

The Drawer Motion test checks the drawer open and close opto functionality and drawer
movement. Failure of this test could indicate a faulty or misalign open or close optos, a bad
motor or power control board.

4.1.9 Print Button

The Print button allows the user to print the QC Tests results.

4.1.10 Exit Button

The Exit button exits the user out of the QC Tests [Self Tests] Tab and returns the user to the
Main Menu.

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5 Control

Figure 5.1 Control Tab

The Control Tab (Figure 5.1) is used to control the movement and test the Color Wheel and Drawer
functionality, as well as the Open and Closed Drawer optos.

5.1 Colorwheel

Figure 5.2, Control – Filter Motion Box

The Filter Motion Box (Figure 5.2) allows the user to select any of the color wheel filters in the Select
Specific Filter box. Selecting a filter radio button advances the color wheel to the selected filter.

5.1.1 Select Specific Filter

The Select Specific Filter box enables the user to select any one of the colorwheel filters
listed in (Table 5.1) by selecting the radio button for a particular filter. An opto-interrupter
is used to send a feedback signal to the CPU when a filter is placed in the correct light path.
The CPU then stops the color wheel motor. A failure in this test could indicate a defective

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 opto-interrupter, motor drive circuitry or mechanical assembly. Use this Tab to check the
colorwheel filter and home positions.

5.1.2 Home
The Home radio button enables the user to home the colorwheel. The opto-interrupter is
used as a feedback device to the CPU Board. A failure in this test could indicate a defective
opto-interrupter, motor drive circuitry or mechanical assembly. It is recommended to
initially home the color wheel before advancing through the filters.
Wavelength Color
620nm Red
560nm Green
505nm Blue-Green (Aqua)
470nm Dark Purple
440nm Blue
590nm Yellow
Table 5.1, Colorwheel Filter Table

The Colorwheel Filter (Table 5.1) lists each filter and it’s wavelength and color. The
Home filter tab reads the black metal slug placed in filter 7 position. Selecting the filter
radio button moves the color wheel to the selected filter.

5.1.3 Continuous Filter Motion

The Continuous Filter Motion check box allows the user to cycle continuously through each
color wheel filter. “Seconds between movements” controls the filter selection rate. Total
Cycles updates each time a filter is selected.

5.1.4 Total Cycles

Indicate how many times the color wheel has advanced to a new filter. It also displays which
filter the color wheel is reading.

5.1.5 Seconds Between Movements

Enables the user to enter a time delay between each filter selection.

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 5.2 Drawer

Figure 5.3, Control – Drawer Motion Box

The Drawer Motion Box (Figure 5.3) allows the user to manually or continuously open and close the
drawer. This tab queries the autoSCAN-4 Instrument to determine the current drawer position.

5.2.1 Select Specific Position

The Select Specific Position contains the Open and Closed drawer radio buttons.

5.2.1.1 Open Radio Button

Selecting the Open radio button causes the drawer to be open. When the drawer opens or
closes, opto-interrupters are blocked or unblocked, and this signal is sent to the CPU.

5.2.1.2 Closed Radio Button

Selecting the Closed radio button causes the drawer to closed. When the drawer opens or
closes, opto-interrupters are blocked or unblocked, and this signal is sent to the CPU.

5.2.2 Continuous Motion

The Continuous Motion check box allows the user to open and close the drawer
continuously. The enabled feature initiates the continuous drawer open and close process,
the seconds between movements’ controls the drawer open and closure rates. Each time the
drawer is opened and closed is considered one cycle. The total numbers of cycles are
displayed in the Total Cycles counter.

5.2.3 Total Cycles

Indicates the number of times the drawer has opened and closed.

5.2.4 Seconds Between Movements

Enables the user to set the time delay between drawer open and close positions.

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 5.2.5 Position Indicator
Displays drawer’s current Open / Close drawer position.

5.2.6 Closed Opto Indicator

Displays drawer Closed Opto position. Blocked indicates the drawer is in the closed
position. Clear indicates drawer is in the open position.

5.2.7 Open Opto Indicator

Displays drawer Open Opto position. Blocked indicates the drawer is in the open position.
Clear indicates drawer is in the closed position.

6 Alignment
The Alignment Tab (Figure 6.1) is used to align the autoSCAN-4 Power Supply Voltages, Reference
Fiber, A/D Board and Offset Test Panel. Each Alignment Tab contains a Next Step, Last Step and Exit
button. The Next Step button enables the user to move forward to the next Step in the alignment process.
The Last Step button allows the user to move back to the previous Step. The Exit button will exit the user
out of the Alignment Tab and return the user to the Main Menu.

6.1 Power Supply

The Power Supply Tab (Figure 6.1) enables the user to adjust the +12.3 volt, +5 volt, +15 volt and
–15 volt power supply voltages. The main power supply for the autoSCAN-4 Instrument is the
12.3 volt power supply located under the drawer. LabPro autoSCAN-4 Diagnostics does not
monitor this power supply. The 12.3 volts is adjusted using a voltage meter and a non-conductive
screwdriver. The 12.3 volts is applied to the Power Control Board and tungsten halogen lamp. The
power board converts the 12.3 volts to +/-15 volts and +5 volts on the Power Controller Board.
The 12.3-volt power supply must be set properly to ensure the validity of all other voltage
adjustments.

Figure 6.1, Alignment – Power Supply Tab

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 6.1.1 Step 1

Figure 6.2, Alignment – Power Supply – Step 1

NEW PCB: using a voltmeter, measure between TP1 (12 volts) and TP2 (ground) on the Power Controller
Board. Using a non metallic screwdriver adjust R26 on the 12-volt power supply for 12.3 volts +/- 0.05
volts.
OLD PCB: using a voltmeter, measure between TP1 (ground) and TP4 (12 volts) on the Power Controller
Board. Using a non metallic screwdriver adjust R25 on the 12-volt power supply for 12.3 volts +/- 0.05
volts.
NOTE: The Power Supply is located under the drawer on the left front side.

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 6.1.2 Step 2

Figure 6.3, Alignment – Power Supply – Step 2

Step 2 is used to adjust R22 on the Power Controller Board for +5.00 (+/- .25) volts.

6.1.3 Step 3

Figure 6.4, Alignment – Power Supply – Step 3

Step 3 is used to adjust R20 on the Power Controller Board for +15.00 (+/- 1volt).

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 6.1.4 Step 4

Figure 6.5, Alignment – Power Supply – Step 4

Step 4 is used to adjust R21 on the Power Controller Board for -15.00 (+/- 1 volt).

6.2 Reference Fiber

The Reference Fiber Tab (Figure 6.6) allows the user to align the reference fibers. The reference
fibers are the two brightest fibers, using the 620nm or 560nm filters. The log amplifier computes
the logarithm of the ratio of the signal voltage to the reference voltage. This eliminates unwanted
detected signal transients and fluctuations. These signals and reference voltages are amplified on
the A/D board.

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 Figure 6.6, Alignment – Reference Fiber Tab

6.2.1 Step 1

Figure 6.7, Alignment – Reference Fiber – Step 1

Step 1 gives the user a place to check the diffuser and photo diode shields are clean and in place. Ensure
the frosted side of the diffuser plate is facing up.

6.2.2 Step 2

Figure 6.8, Alignment – Reference Fiber – Step 2

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 Step 2 displays the output of each colorwheel filter. The two filters with the highest output must be one of
the first two filters (620nm or 560nm). If this is not the case, all filters must be examined for dust, dirt and
contamination and/or replaced until the first two filters have the highest output. The user adjusts R3 on the
photo board until the highest filter reads 1.100 +/- 0.010 volts.

6.2.3 Step 3

Figure 6.9, Alignment – Reference Fiber – Step 3

Step 3; the user will adjust the higher (highlighted) of the first two filters (620nm or 560nm) in Step 2 to
1.100 (+/- 0.010) volts by adjusting R3 on the Photo Board.

6.3 A/D Board

The A/D Board Tab enables the user to align the A/D board. This is done to ensure the analog
signals generated by the photo board and the reference voltages are amplified in a uniform manner
on the A/D board.

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 Figure 6.10, Alignment – A/D Board Tab

6.3.1 Step 1

Figure 6.11, Alignment – A/D Board – Step 1

Step 1 is used to make the Preliminary Gain Adjustment by adjusting R4 on the A/D Board to 10.000 volts
(+/- 0.010 volts). The signal level that is being generated and DAC circuitry can be monitored at test point
11 on the A/D board.

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 6.3.2 Step 2

Figure 6.12, Alignment – A/D Board – Step 2

Step 2 is used to make the Preliminary Offset Adjustment by adjusting R3 on the A/D Board to 0.000 volts
(+/- 0.002) volts. As in step 1, the signal can be monitored at test point 11 on the A/D Board.

6.3.3 Step 3

Figure 6.13, Alignment – A/D Board – Step 3

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 Step 3 is used to make the Final Gain Adjustment by adjusting R4 on the A/D Board to 0.000 volts (+/-
0.002) volts. The digital signal being generated out of the A/D integrated circuit and its tolerances are
displayed on the screen. The signal level is present at test point 14 on the A/D board.

6.3.4 Step 4

Figure 6.14, Alignment – A/D Board – Step 4

Step 4 is used to make the Final Offset Adjustment by adjusting R36 on the A/D Board to an output that
reads between 9.995 and 10.000 volts. The signal level and tolerance out of the Log Amp is displayed on
the screen. The signal is present at test point 14 on the A/D board.

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 6.3.5 Step 5

Figure 6.15, Alignment – A/D Board – Step 5

Step 5 is used to verify the Slope reading is between –9.900 and –10.400 volts. The signal level is located
at test point 14 on the A/D board. If the voltage is out of range press the Last Step button to move back to
Step 1 of the alignment procedure and readjust the voltages in each Step.

6.4 Offset Test Panel

The Offset Test Panel Tab is used to perform the final offset adjustment. When the user presses
Next Step to go to Step 2 the autoSCAN-4 Instrument will perform a calibration. Then the user
must establish the target value and date for the offset test panel and enter it in Step 2. Check the
Date box in Step 2 if a date is stamped on the offset test panel. If not leave the box unchecked. If
actual value reading is not with in +/- 20 counts of the target value adjust R3 and re-read the offset
test panel.

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 Figure 6.16, Alignment – Offset Test Panel Tab

6.4.1 Step 1

Figure 6.17, Alignment – Offset Test Panel – Step 1

Step 2; informs the user the autoSCAN-4 Instrument must be re-calibrated. Clean the shields and diffuser
and ensure they are in place with the diffuser’s frosted side up. Also ensure the drawer is empty. Select
Next Step to move to Step 2 and calibrate the instrument.

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 6.4.2 Step 2

Figure 6.18, Alignment – Offset Test Panel – Step 2

Please wait while the autoSCAN-4 Instrument is calibrating.

Figure 6.19, Alignment – Offset Test Panel – Step 2B

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 This screen is displayed when the calibration is completed and the autoSCAN-4 Instrument is ready to
read the offset test panel. Enter the offset test panel number printed on the panel, if a date is also stamped
on the panel check the date stamp box. Press the Next Step button to read the offset test panel.
NOTE: No date etched in the corner of the panel Subtract 223 from the Target Value etched in on comer of the
panel.
Example: Offset Test Panel (Target Value 1022) – 223 = 799 new Target Value.

Has a date etched in the corner with Target Value, do not subtract 223. Target value is the number etched
in the comer of the panel.
Example: Offset Test Panel (Target Value 1022) = 1022 new Target Value.

6.4.3 Step 3

Figure 6.20, Alignment – Offset Test Panel – Step 3

Step 3; the Offset Test Panel is being read by the autoSCAN-4 Instrument. All filters are read
during the offset test panel read. When the read is completed compare the Read Value to the
Target Value and if the value is within 20 counts of the Target value the alignment is complete.

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 Figure 6.21, Alignment – Offset Test Panel

Compare the Target Value with the Read Value, if the Read Value is within specifications, (+/- 20 counts
of the Target Value) the adjustment is complete. If the Read Value is out of range then adjust R3 and press
the Re-read Panel button to read the offset test panel again.
Note: Adjust R3 on the A/D board clockwise to decrease the Read Value number.
Adjust R3 on the A/D board counter clockwise to increase the Read Value number.

Figure 6.22, Alignment – Offset Test Panel – Step 3C

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7 Test
The Tests Tab (Figure 7.1) allows the user to test the PROM Checksum, RAM Memory, Lamp voltage and
Drawer movement. The user selects each test individually by checking the box next to the test. Press the
Run button to execute the selected test or tests. View the test results in the Results Box. Print the test
results by selecting Print Results button.

Figure 7.1, Test Tab

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 7.1 PROM Checksum

Figure 7.2, Test – PROM Checksum

The PROM Checksum check box enables the user to select the PROM Checksum test. Once
selected pressing the Run button will execute the test. The results will be displayed in the Results
box. If the reader program (which controls the internal working functions of the instrument) has
been corrupted, the PROM checksum test should indicate this as a failed component. The CPU
board should be replaced.

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 7.2 RAM Memory

Figure 7.3, Test – RAM Memory

The RAM Memory box allows the user to test the RAM Memory. All panel data gathered by the
CPU is temporarily stored in RAM before being sent to the computer. A RAM memory failure
requires replacing the CPU board assembly.

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 7.3 Lamp

Figure 7.4, Test – Lamp

The Lamp box allows the user to test the color wheel lamp. By selecting Lamp the system will
give an indication of whether the lamp is operating or burned out. The color wheel should
advance to a designated location and the user should observe a color from the scanning indicator.
A failure in this test indicates a defective lamp.

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 7.4 Drawer

Figure 7.5, Test – Drawer

The Drawer box when checked opens and closes the drawer three times. This function is used to
check the drawer open and close opto functionality and drawer movement. Failure of this test
could indicate a faulty or misalign open and/or closed drawer opto, or a bad motor or power
controller board.

7.4.1 Cycle ? of List Box

The Cycle ? of box allows the user to enter the number of times the selected test or tests will
be run.

7.4.2 Clear Tests Button

The Clear button allows the user to deselect all checked boxes in the Selections Box.

7.4.3 Run Button

The All button allows the user to select all checked boxes in the Selections Box.

7.4.4 Cancel Button

Terminates the Test Tab and returns the user to autoSCAN-4 LabPro Diagnostics Main
Menu.

7.4.5 Print Results Button

The Print Results box allows the user to print the test results.

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 7.4.6 Results Box
The Results box displays the pass/fail results of the tests run in the Selections Box.

8 File
The File Drop down box (Figure 8.1) contains the Log, Parameters, Password and Exit items. The Log
lists all the events that have occurred on the autoSCAN-4 Instrument. The Parameters file lists the
autoSCAN-4 Instrument to Computer communication parameters. The Password launches the
autoSCAN-4 Diagnostics Password dialog box. Exit terminates autoSCAN-4 Diagnostics.

Figure 8.1, File Drop Down Box

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 8.1 Log

Figure 8.2, File – Log

The Log records events taken place on the autoSCAN-4 Instrument.

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 8.2 Parameters

Figure 8.3, File – Parameters

The Parameters box contains the Timer Test and Parameters boxes. The Timer Test is used to set
up the communication start and stop times. The Parameters is used to set up the communication
parameters used to communicate between the autoSCAN-4 Instrument and the computer system.
The default parameters shall be used in the communication between the autoSCAN-4 Instrument
and the computer. These parameters shall not be altered unless directed to by Dade Behring
Microscan Technical Support.

8.3 Password
Selecting the Password item will execute the LabPro autoSCAN-4 Diagnostics password screen
as shown in Figure 8.4. This Password screen is used to log on to LabPro autoSCAN-4
Diagnostics.

Figure 8.4, File – Password

8.4 Exit
Selecting the Exit will terminate LabPro autoSCAN-4 Diagnostics.

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