Mispa Count Plus Service Manual

DOC No.
ADL/TSD/MC PLUS/SER/001
DOCUMENT NAME- Service manual
EQUIPMENT MODEL- Mispa Count Plus
AGAPPE DIAGNOSTICS LTD
YOUR BEST PARTNER IN DIAGNOSTICS 1

DOC No. ADL/TSD/MC PLUS/SER/001
REVISION RECORD
REV REASON FOR THE PAGE
No. DATE REVISION AUTHOR CHANGED S/No
1 11/04/2018 JIJU VARGHESE -- 50---------
DISRIPTION
PAGE
S/No. DIVISION/ PART DETAILS NUMBER

Chapter 1 Safety Guidance……………………………………………………………………………………………………………………………………….…8

Symbols and Definitions…………………………………………………………………………………………………………………………………...…………8
1.2 Warnings and Cautions………………………………………………………………………………………………………………………………………….8
Chapter 2 General Overview……………………………………………..…………………………………………………………………………………………10
2.1 Introduction…………………………………………………..………………………………………………………………………………………….…………..10
2.2 Main Parts…………………………………………………………………………………………………………..……………………………………………..12
2.2.1 Front cover………………………………………………………………………………………………………………………………………………...….12
2.2.2 Fluidic part……………………………………………………………………………………………………………………….…13
2.2.3 Reagent area………………………………………………………………………………………………………………….…..14
2.2.4 Connection board………………………………………………………………………………………………………….……14
2.2.5 External power supply block…………………………………………………………………………………………….15
2.2.6 Printer (optional)TBD…………………………………………………………………………………………………..……15
2.2.7 External Barcode reader (optional)…………………………………………………………………………………15
2.3 Configuration…………………………………………………………………………………………………………………….……16
2.3.1 Standard Configuration…………………………………………………………………………………….………………16
2.3.2 Options……………………………………………………………………………………………………………………….……….16
Chapter 3 Installation Guidance………………………………………………………………………………………….……16
3.1 Installation Requirement………………………………………………………………………………………………….….16
3.1.1 location………………………………………………………………………………………………………………………………..16
3.1.2 Installation environment………………………………………………………………………………………………..…16
3.2 Unpacking………………………………………………………………………………………………………………………..…….17
3.2.1 Unpacking Procedure……………………………………………………………………………………………………….17
3.2.2 Visual check……………………………………………………………………………………………………………………....17
3.3 Installation………………………………………………………………………………………………………………………….….19
3.3.1 Electric and Hydraulic connections…………………………………………………………………………………19
3.4 STARTUP………………………………………………………………………………………………………………………….……23
3.3.2.1 Rights of access……………………………………………………………………………………………………….…….25
Chapter 6 Technology…………………………………………………………………………………………………………………27
6.1.1 Detection Principle WBC, RBC, PLT Counting………………………………………………………………27
6.1.2 Five-part diff measurement……………………………………………………………………………………………..28
6.2 Haemoglobin Measurement………………………………………………………………………………………………..29
6.3 Leukocyte Analysis…………………………………………………………………………………………………………....…30
6.4 Erythrocyte Analysis………………………………………………………………………………………………………….….31
6.5 Platelet Analysis……………………………………………………………………………………………………………….…..33
6.6 Alarms…………………………………………………………………………………………………………………………………..…36
6.6.1 General Flags……………………………………………………………………………………………………………………..36
6.6.2 Leukocytes Flags………………………………………………………………………………………………………………37
6.6.3 Erythrocyte and HGB Flags…………………………………………………………………………………………..…39
6.6.4 Platelet Flags……………………………………………………………………………………………………………………..40
6.6.5 System Alerts………………………………………………………………………………………………………………… ….41
6.6.5.1 INS-T / Instrument Temperature Out Of Range………………………………………………………..41
6.6.5.2 WBC BAL / WBC Balance………………………………………………………………………………………….….41
6.6.5.3 WBC-CL / WBC Aperture Clog……………………………………………………………………………..………41
6.6.5.4 RBC-CL / RBC Aperture Clog……………………………………………………………………………………….41
6.6.5.5 O-DF / Optical Default……………………………………………………………………………………………..……41
6.6.5.6 SU-F / Startup Failed…………………………………………………………………………………………………....41
6.6.5.7 QC Not Done / QC Not Done During The Day……………………………………………………………41
6.6.5.8 QC Failed / Last Run QC Failed…………………………………………………………………………………..41
6.7 Hydraulic Description…………………………………………………………………………………………………………..42
6.7.1 Sampling module…………………………………………………………………………………………………….…………43
6.7.2 syringe module…………………………………………………………………………………………………………..………43
6.7.3 Syringe valve module…………………………………………………………………………………………………........43
6.7.4 Counting module……………………………………………………………………………………………………………….……………………….…43
6.7.5 Optic Bench………………………………………………………………………………………………………………..………43
6.8 Software………………………………………………………………………………………………………………………….……..43

6.8.1 Graphical User Interface…………………………………………………………………………………………..………43

6.8.2 Windows……………………………………………………………………………………………………………………………...44
Chapter 7 Specifications……………………………………………………………………...…………………………..………………………….45
7.1 Analytical Specifications…………………………………………………………………………………………………..…45
7.1.1 Linearity………………………………………………………………………………………………………………………..…….45
7.1.2 Background…………………………………………………………………………………………………………………………46
7.1.3 Precision………………………………………………………………………………………………………………………….….46
7.1.4 Carry-Over…………………………………………………………………………………………………………………………..47
7.1.5 Correlation……………………………………………………………………………………………………………………..……48
7.2 Units………………………………………………………………………………………………………………………………..…….49
7.3 Reagent consumption…………………………………………………………………………………………………….…..50
7.4 Physical Specifications………………………………………………………………………………………………………..50
7.4.1 Instrument Specifications………………………………………………………………………………………………..51
7.4.2 Power Supply Block………………………………………………………………………………………………………..…51
7.5 Reagents Specifications……………………………………………….……………………………………………………..52
7.5.1 MISPACOUNTPLUS Diluent (DIL-5D)…………………………………………………………………………..52
7.5.2 MISPACOUNTPLUS Cyanide Free Lytic Solution (Lyse5D) ………………………………………52
7.5.3 MISPACOUNTPLUS Enzymatic Cleaning Solution (Cleaner)…………………………………………52
7.3 Reagent consumption…………………………………………………………………………….53
7.6 Analytical Limitations…………………………………………………………………………..54
7.6.1 Interferences…………………………………………………………………………………………………………54
Chapter 9 SERVICE………………………………………………………………………….55
9.1 Introduction……………………………………………………………………………………………55
9.2 SYSTEM INIT…………………………………………………………………………………………….55
9.3 LOGS……………………………………………………………………………………………………….56
9.3.1 Event logs………………………………………………………………………………………………………………….56
9.4 ERROR LOGS…………………………………………………………………………………………….56
9.5 BACKUP & RESTORE……………………………………………………………………………….57
9.5.1 BACKUP……………………………………………………………………………………………………………………57
9.5.2 RESTORE……………………………………………………………………………………………………………………57
9.6 SETTINGS………………………………………………………………………………………………..58
9.6.1 LAB PARAMETERS……………………………………………………………………………………………………..58
9.6.1.1 LAB PREFERENCE…………………………………………………………………………………………………….59
9.6.1.2 UNITS………………………………………………………………………………………………………………………61
9.6.1.2.1 US Format & units………………………………………………………………………………………………..61
9.6.1.2.2 SI Format & units……………………………………………………………………………………………….61
9.6.1.2.3 SI MODE Format & units:……………………………………………………………………………………62
9.6.1.2.4 Japanese Format & units:…………………………………………………………………………………..62
9.6.1.3 CBC THRESHOLDS A FLAGS………………………………………………………………………………….63
9.6.1.4 DIF THRESHOLDS AND FLAGS………………………………………………………………………………63
9.6.1.5 REFERENCE RANGES……………………………………………………………………………………………64
9.6.1.6 Calibration Factors……………………………………………………………………………………………….65
9.6.2 DATE/TIME……………………………………………………………………………………………………………………….66
9.6.3 AUTOMATIC CYCLES………………………………………………………………………………………………….67
9.6.4 PRINTER…………………………………………………………………………………………………………………68
9.6.4.1 PRINTER SETTINGS………………………………………………………………………………………………..68
9.6.4.2 PRINTER MANAGEMENT……………………………………………………………………………………….69
9.6.5 Communication……………………………………………………………………………………72
9.6.6 USERS MANAGEMENT……………………………………………………………………………………………..74
9.6.6.1 Add……………………………………………………………………………………………………………………….74
9.6.6.2 Change Password…………………………………………………………………………………………………..74
9.6.6.3 Remove………………………………………………………………………………………………………………….75
9.6.7 SOFTWARE UPDATE………………………………………………………………………………………………..76
9.7 TROUBLESHOOTING……………………………………………………………………………….77
9.7.1 FLUIDICS CONTROL……………………………………………………………………………….77

9.7.2 BLEACH CLEANING………………………………………………………………………………78

9.7.3 DRAIN BATHS…………………………………………………………………………………………………………79
9.7.4 BACKFLUSH APERTURES…………………………………………………………………………………………79
9.7.5 BACKFLUSH OPT.BENCH………………………………………………………………………………………….80
9.7.6 NEEDLE DISMANTLING…………………………………………………………………………………………….80
9.7.6.1 CHECK ROCKER………………………………………………………………………………………………………81
9.7.7 PARK MOTORS…………………………………………………………………………………………………………..81
9.7.8 RINSE………………………………………………………………………………………………………………………..82
9.7.9 CLEAN………………………………………………………………………………………………………………………82
9.7.9 DRAIN FOR PACK UP…………………………………………………………………………………………………82
9.7.10 SYRINGE GREASING………………………………………………………………………………………………….82
9.7.11 CHECK SENSORS/VALVES…………………………………………………………………………………………84
9.7.12 CHECK SYRINGE………………………………………………………………………………………………………87
9.7.13 CHECK NEEDLE………………………………………………………………………………………………………..87
9.7.15 Maintenance……………………………………………………………………………………………………………………….88
Troubleshooting…………………………………………………………………………………………..89
9.7.16.1 Analytical problems……………………………………………………………………………………………………….89
9.7.16.2 Other problems………………………………………………………………………………………………………………….89
9.7.17 Troubleshooting Messages…………………………………………………………………..90
Chapter 10 SHUTDOWN……………………………………………………………102
Introduction………………………………………………………………………………………………………………………………..103
Error Messages – Trigger, consequence’s, Trouble Shooting……………………………………….103
Mispa Count Plus Analyse Cycle - Fluidic Sequence Description……………………………….125
Dismantling of different Units……………………………………………………………………………………………..130
Vacuum Tank………………………………………………………………………………………………………………………….131
Pump………………………………………………………………………………………………………………………………………..132
Heating system……………………………………………………………………………………………………………………….135
Optical Module Disassembly………………………………………………………………………………………………..136
Optical Module Replacement………………………………………………………………………………………………137
Optical Module Dismantling………………………………………………………………………………………………….137
Optical Module Assembling……………………………………………………………………………………………………142
Optical module Adjustment………………………………………………………………………………………………..145
Optical Module……………………………………………………………………………………………………………………..153
Illumination Part…………………………………………………………………………………………………………………..154
Detection Part………………………………………………………………………………………………………………………154
Flow cell………………………………………………………………………………………………………………………………..155
Counting Module Disassembly………………………………………………………………………………………….157
RBC Electrode………………………………………………………………………………………………………………………157
RBC Counting Chamber replacement………………………………………………………………………………158
WBC Counting Head Replacement……………………………………………………………………………………161
WBC Electrode Replacement ……………………………………………………………………………………………164
WBC Counting Chamber Replacement……………………………………………………………………………….…165
WBC Counting Head Replacement …………………………………………………………………………………….…168
Counting Holder………………………………………………………………………………………………………………………..171
Heater Module Disassembly…………………………………………………………………………………………………..173
Heater Replacement……………………………………………………………………………………………………………….173
Heater Dismantling…………………………………………………………………………………………………………………173
Heater Assembling………………………………………………………………………………………………………………….175
Inline Pressure Sensor Disassembly………………………………………………………………………………….….177
Inline Pressure Sensor Replacement……………………………………………………………………………….……177
Inline Pressure Sensor Dismantling……………………………………………………………………………………..177
Inline Pressure Sensor Assembling………………………………………………………………………………….……178
Pump Disassembly…………………………………………………………………………………………………………………180
Pump Replacement………………………………………………………………………………………………………………….180
Rinse pump dismantling…………………………………………………………………………………………………………..180

Rinse pump assembling…………………………………………………………………………………………………………183

Stopcock Disassembly…………………………………………………………………………………………………………….185
Stopcock Replacement……………………………………………………………………………………………………………185
Stopcock Dismantling………………………………………………………………………………………………………………185
Stopcock Assembling………………………………………………………………………………………………………………187
Ambient Temperature Sensor Disassembly………………………………………………………………………189
Ambient Temperature Sensor Replacement………………………………………………………………………189
Ambient Temperature Sensor Dismantling…………………………………………………………………………189
Ambient Temperature Sensor Assembling………………………………………………………………………….191
Diluent Temperature Sensor Replacement………………………………………………………………………..192
Diluent Temperature Sensor Dismantling…………………………………………………………………………..192
Diluent Temperature Sensor Assembling…………………………………………………………………………….194
Discharge Tubing Disassembly……………………………………………………………………………………………..195
Discharge Tubing Replacement……………………………………………………………………………………………195
Discharge Tubing Dismantling……………………………………………………………………………………………..195
Discharge Tubing Assembling……………………………………………………………………………………………..197
CPU Board Disassembly…………………………………………………………………………………………………………199
CPU Board Replacement………………………………………………………………………………………………………199
CPU Board Dismantling………………………………………………………………………………………………………..199
CPU Board Assembling………………………………………………………………………………………………………….201
USB Board Replacement……………………………………………………………………………………………………….205
USB Board Dismantling……………………………………………………………………………………………………….205
USB Board Assembling………………………………………………………………………………………………………..206
Rear Panel board replacement……………………………………………………………………………….………….208
Rear Panel board dismantling……………………………………………………………………………….…………….208
Rear Panel board assembling……………………………………………………………………………………………….210
HGB Board replacement…………………………………………………………………………………….………………….212
HGB Board dismantling……………………………………………………………………………..……………………………212
HGB Board assembling……………………………………………………………………………………………………………213
Sampling Module Disassembly…………………………………………………………………………………………..…..214
Sampling probe replacement…………………………………………………………………………………………………..214
Probe dismantling…………………………………………………………………………………………………………………..…214
1.2 Probe assembling……………………………………………..…………………………………………………………….….217
Probe’s O-ring replacement…………………………………………………………………………………………………….220
2.1 Probe’s O-ring dismantling………………………………………………………………………………………….…..220
Syringe Module Disassembly…………………………………………………………………………………………….…..221
Syringe body & Pistons replacement……………………………………………………………………………….……221
Syringe body & pistons dismantling………………………………………..………………………………..………….221
Syringe body & pistons assembling…………………………………………………………………………………….…223
Syringe motor holder replacement………………………………………………………………………………………..226
Syringe motor holder dismantling………………………………………………………………………………….………226
Syringe motor holder assembling……………………………………………………………..………………..………….229
Slotted optical sensor replacement……………………………………………………………………………………….232
Slotted optical sensor dismantling………………………………………………………………………………………..232
Counting Valves Module Disassembly………………………………………………………………………….…..….233
Counting valves replacement……………………………………………………………………………………….…..……233
Counting Valves Module Replacement…………………………………………………………..…………….………235
Slotted optical sensor assembling………………………………………………………….…………………………..237
2.2 Probe’s O-ring assembling………………………………………………………………………………………………..240
3 Rocker replacement………………………………………………………………………………………………………………243
3.1 Rocker dismantling……………………………………………………………………………………………………..…….243
3.2 Rocker assembling……………………………………………………………………………………………………..…….250
4 Probe belt & probe carriage replacement…………………………………………………………………………261
4.1 Probe belt & probe carriage dismantling……………………………………………………………………..261
1.1 Belt and probe carriage assembling…………………………………………………….…………………………265

3 Probe’s sensor board replacement……………………………………………………………………………..…….269

3.1 Probe’s sensor board dismantling………………………………………………………………………………..269
3.2 Probe’s sensor board assembling…………………………………………………………………………..………271
4 Probe stepper motor replacement……………………………………………………………………………………272
4.1 Probe stepper motor dismantling………………………………………………………………………………….272
4.2 Probe stepper motor assembling……………………………………………………………………………………273
5 Rocker stepper motor replacement…………………………………………………………………………………..275
5.1 Rocker stepper motor dismantling………………………………………………………………………………..275
5.2 Rocker stepper motor assembling…………………………………………………………………………………..279
6 Rocker optical sensor replacement………………………………………………………………………………....281
6.1 Rocker optical sensor dismantling……………………………………………………………………………….…281
6.2 Rocker optical sensor assembling….…………………………………………………………………………..……284
Syringe Valves Module #1 Disassembly…………………………………………………………………….………….285
Syringe Valves module #1 replacement….……………………………………………………………………………287
Syringe Valves module #1 dismantling……………………………………………………………………………….287
Syringe Valves module #1 assembling…..………………………………………………………………………….….289
Syringe Valves Module #2 Module Disassembly.. …………………………………………………..…………290
Syringe valves (from syringe Valves Module #2) replacement……………………………..…………290
Syringe Valves module #2 replacement………………………………………………………………….……………292
Syringe Valves module #2 dismantling………………………………………………………………………………….292
Syringe Valves module #2 assembling………………………………………………………………………….………294
Syringe Valves module #3 Disassembly………………………………………………………………………..………295
Syringe Valves module #3 replacement. ………………………………………………………………………………295
Syringe Valves module #3 dismantling….………………………………………………………………………..…….295
Syringe Valves module #3 assembling………………………………………………………………………….………297
Fluidic system………………………………………………………………………………………………………………….………..298
MISPACOUNTPLUS Sampling……………………………………………………….…………………………………….325
Optic back flush……………………………………………………………………………………………………………..………….326
RBC bubbling…………………………………………………………………………………………………………………….……….327
WBC bubbling…………………………………………………………………………………………………………………………….329


Chapter 1 Safety Guidance
1.1 Symbols and Definitions
WARNING RISK OF DANGER. Indicates a procedure to be strictly respected in order to

avoid any risk for the user, damage on the instrument or on the result’s quality.
Indicates that wearing gloves is mandatory before performing the described operation
due to risk of contact with materials that may be infectious.
NOTE Indicates important information
Indicates that this product may not be treated as household waste. Instead it shall be
handed over the applicable collection point for the recycling of electrical and electronic
equipment. By ensuring this product is disposed of correctly, you will help prevent
potential negative consequences for the environment and human health, which could
otherwise be caused by inappropriate waste handling of this product. For more
detailed information about recycling of this product, please contact your local city
office or your distributor of this product.
1.2 Warnings and Cautions

NOTE: Misuse of electrical equipment may cause electrocution, burns, fire and other hazards.
Check if the voltage setting matches the supply voltage.

Connect MISPACOUNTPLUS power supply block to a main power supply outlet with an earth connection.
Preserve a good access to the supply outlet to be able to unplug MISPACOUNTPLUS in emergency case.
Do not place the power supply adapter in liquid, nor put it where it could fall into liquid. If the power
supply adapter becomes wet, unplug it before touching it.
Do not use MISPACOUNTPLUS if it is not working properly, or if it has suffered any damage (damage to
the supply cord or its plug; damaged caused by dropping the power supply adapter).
Do not let the power supply adapter or its flexible cord meet surfaces which are too hot to touch.
Do not place anything on top of MISPACOUNTPLUS
Do not use MISPACOUNTPLUS where aerosol sprays are being used, or where oxygen is being
administered.
Do not use MISPACOUNTPLUS outside
Always switch off MISPACOUNTPLUS and disconnect the power adaptor before dismantling any part.
NOTE: MISPACOUNTPLUS is an automated hematology analyzer for in vitro diagnostic use in clinical
laboratories by a representative people.
Only human blood or artificial control and calibration blood must be run.

The optimum performances can be only achieved if the cleaning and maintenance procedures are
strictly respected.
Due to the use and the environment of this equipment, all parts and surfaces of MISPACOUNTPLUS are
potentially infective. Wearing gloves and hands washing after work completion are strongly
recommended.
Always replace or use parts of the equipment by original parts.
Basic safety precautions should always be taken. If the equipment is not used per the manufacturer’s
instructions, the protective by the equipment may be impaired.
The treatment of waste and the elimination of a part or the complete instrument must be done in
compliance with the local legislation.
Any output or input connections (except the printer and the barcode reader) cannot be done without
representative authorization.
Do not open the door located on the right side of the instrument when hydraulic cycle is in progress, it will
lead to an immediate stop.

Chapter 2 General Overview
2.1 Introduction
MISPACOUNTPLUS is a fully automated analyzer performing hematological analysis on whole blood
collected on EDTA tubes.
Sampling volume is 15.6 µl and analysis cycle duration is 60 seconds.
NOTE: Result is displayed and printed before the end of the analysis cycle.
Here below the list of all parameters determined by the analyzer for each analysis:
White blood cell parameters:

Determination
Symbol Description
WBC Total Count Measured
LYM%/# Lymphocyte percent and absolute value Measured/Calculated
MON%/# Monocyte percent and absolute value Measured/Calculated
NEU%/# Neutrophil percent and absolute value Measured/Calculated
EOS%/# Eosinophil percent and absolute value Measured/Calculated
BAS%/# Basophils percent and absolute value Measured/Calculated
ALY%/#* Abnormal Lymphocyte percent and absolute value Measured/Calculated
IMM%/#* Immature Monocyte percent and absolute value Measured/Calculated
Red blood cell parameters:

Determination
Symbol Description
RBC Total count Measured
HGB Hemoglobin Measured
HCT Hematocrit Calculated
MCV Mean Cell Volume Calculated
MCH Mean Cell Hemoglobin Calculated
MCHC Mean Cell Hemoglobin Concentration Calculated
RDW-CV Red Blood Cells Distribution Width-CV Calculated
RDW-SD Red Blood Cell Distribution Width-SD Calculated
Platelet parameters:
Determination
Symbol Description
PLT Total count Measured
MPV Mean Platelet Volume Calculated
PCT* Plateletcrit Calculated
PDW* Platelets Distribution Width Calculated
P-LCR* Large Platelets Counts Ratio Calculated

Note: Parameters followed with (*) are RUO (Research Use Only), to be displayed the option must be
activated.

2.2 Main Parts

MISPACOUNTPLUS consists of the seven main parts listed below:
1. Front cover.
2. Fluidic part.
3. Reagent area.
4. Connection board.
5. External power supply block.
6. Printer (optional).
7. External Barcode reader.
2.2.1 Front cover

The front cover consists of the five elements listed below:
1. Touch screen LCD display (8.4”).

2. ON/OFF & EMERGENCY STOP button.
3. Start analysis cycle trigger.
4. USB port connection.
5. CPU board located behind the screen

2.2.2 Fluidic part

The fluidic part is located on the right side of the instrument directly accessible after opening of the fluidic
door.
The fluidic part consists of the six elements listed below:
1. Sampling module
2. Syringe module
3. Syringe valve module
4. Counting module
5. Counting valve module
6. Optic Bench
1
3

2.2.3 Reagent area

The reagent area is located on the left side of the instrument directly accessible after opening of the reagent
door. The reagent area is dedicated for lyse & cleaner reagent bottles.
2.2.4 Connection board

The connection board is located at the back of the instrument and allows different types of connections
described below.
4 USB Ports
TP/TCIP Port
Serial Link RS232 Port
Not Used
P/S 24V

2.2.5 External power supply block

MISPACOUNTPLUS is supplied with an external power supply block.
• 100-240 VAC
• 50/60 Hz
• Single phase with ground
In the case of replacement of the main power cord supplied with MISPACOUNTPLUS,
the new cord must comply with the local regulation.
MISPACOUNTPLUS has been certified with the power supply block provided with. Use
with another external power supply block is not guaranteed.
2.2.6 Printer (optional)TBD

MISPACOUNTPLUS is not delivered with the printer. The instrument is equipped already with PCL3 & PCL6
standard printer drivers which cover a large choose of printer models. Here below the list of the printers
compatible with the instrument.
2.2.7 External Barcode reader (optional)

An external barrcode reader can be provided as an option (Model OPTICON - C37).
Connected to an USB port of MISPACOUNTPLUS, it allows entering automatically the following fields:
 Sample identification SID.

 Reagent identification (LYSE, CLEANER and DILUENT).
 QC and calibration lot number.
NOTE: The Automatic barcode identification is available only for these fields.

2.3 Configuration
2.3.1 Standard Configuration
 1 analyzer MISPACOUNTPLUS
 1 power cord
 1 power supply block
 1 flat screwdriver
 1 user manual
 1 certificate of approval
 1 diluent pick up tubing
 1 waste tubing
 Maintenance kit (composition TBD)
2.3.2 Options
 1 printer with USB connection

 1 USB Barcode scanner
 1 USB Keyboard (QWERTY only)
Chapter 3 Installation Guidance
3.1 Installation Requirement

3.1.1 location
To ensure MISPACOUNTPLUS fulfills its function, it must be installed on strong and stable table which
supports the weight of the unit as well as the one of the printer and reagents (around 30 Kg). Ten
centimeters space at the rear of the instrument and 30 centimeters on each side are required for
maintenance tasks and reagents change.
Exposure to direct sunlight shall be avoided.
3.1.2 Installation environment

 Indoor use.
 Altitude up to 3000 meters.
 Using temperature [18 °C to 32 °C].
Note: If the ambient temperature moves more than 10°C during the working day, MISPACOUNTPLUS must
be calibrated more frequently.
 Maximum relative humidity for temperatures up to 31 °C is 80 %, decreasing linearly to 50 % at 40 °C.

 Main power supply voltage fluctuations up to ±10 % of the nominal voltage.
 Transient over voltages typically present on the main supply.
 Rated pollution degree II.

Contact your distributor to use the MISPACOUNTPLUS in other conditions than the
ones described above.
3.2 Unpacking
3.2.1 Unpacking Procedure
MISPACOUNTPLUS is delivered in a cardboard. It is recommended to examine the package visually before
unpacking. If there is any sign of mishandling, damage or else, contact the carrier to claim for damage.
During device unpacking, personal in charge of the installation must control the good presence of all
elements needed for installation comparing to the PACKING LIST.
Installation of the device must be done according to the procedure described hereafter.
3.2.2 Visual check

Open the fluidic door on the right side with the screw driver provided in the kit and check the following items:
 Syringes pistons located in top position.

 Sampling module located on front.
 Needle rinsing dismountable system well locked on the sampling module.

If MISPACOUNTPLUS has been stored at a temperature less than 10°C, it must stay
at room temperature during 24 hours to let the time to all elements to reach the
ambient temperature before switching it on.
3.3 Installation
This instruction describes the different stages to follow for the physical installation of MISPACOUNTPLUS
instrument.
3.3.1 Electric and Hydraulic connections.
1. Remove the instrument from the cardboard and place it on a stable table.
2. Remove the accessories boxes from the reagent compartment and unpack the elements.

3. Control the presence of all elements comparing to the packing list
Whether any element would be missing, contact immediately your distributor
4. Connect the diluent pickup tubing to the dedicated hydraulic connector located at the back of the
instrument.
To connect the diluent pick up tubing,

place the two hydraulic connectors face
to face and turn clockwise the one of
the diluent pickup tube
5. Connect the waste tubing to the red hydraulic connector located at the back of the instrument.
To connect the waste tubing, place the

two hydraulic connectors face to face
and turn anticlockwise the red one of
the instrument
6. Tighten the cap of the Lyse pickup tubing (Red color sleeve) and the cap of the Cleaner pickup tubing
(Blue sleeve) to the dedicated bottles.

7. Install lyse and cleaner bottles in the reagent compartment of the instrument.
8. Connect the diluent pickup tubing to the diluent container.
DILUENT CONTAINER MUST ALWAYS BE PLACED ON THE SAME LEVEL THAN THE INSTRUMENT
9. Place the waste straw into a waste container.

WASTE CONTAINER MUST ALWAYS BE PLACED ON FLOOR UNDER THE INSTRUMENT
10. Connect the power supply cable coming from the power supply block to the instrument respecting
the connection way as shown below.
MISPACOUNTPLUS has been certified with the power supply block provided with.
Any use of another external power supply block could not be guaranteed.
11. Connect the main cord to the power supply block.
In the case of change of power cord provided with the instrument, the replacing
cord must be in compliance with the local regulation and the instrument
specification in matter of consumption.
NOTE: The power supply block must be placed at the rear of MISPACOUNTPLUS and if possible, upper than
instrument level to avoid any risk of contact with liquid in case of leak.
12. Connect the main cord to a plug of the main power supply.
• 100-240 VAC
• 50/60 Hz
• Single phase with ground
13. Switch on the instrument pressing on the start button and wait for loading until the display of the
login screen.

The instrument is now physically installed, follow the following chapters of this document to setup all
options needed for putting into operation.
3.4 STARTUP
STARTUP cycle is dedicated to dayly determine the background values of measured parameters.
It must be launched every day before QC and then before patient’s analysis.
Press on .
The front cycle LED turns red , meaning no cycle can be launched before it turns green.
Instrument will proceed first with counting chambers rinsing then, 1 to 3 blank cycles to control the
background values.
NOTE: The background values do not to exceed the following levels:
WBC: 0.2
RBC: 0.02

HGB: 0.2
PLT: 10
DIF#: 100 (number of pulses within the DIF Plot)
If any level from any parameter is higher than expected value, the system warns the user with an alarm
message “STARTUP FAILED”, it is suggested in this case to perform a new start up.
NOTE: If the user chooses to run patient blood after a startup failed, all results will be displayed and printed
with the indication “Startup failed”
Once the startup is performed, the following screen is display in the RESULTS screen.
Only WBC, RBC, HGB, PLT and DIF# are displayed.

3.3.2.1 Rights of access
Depending from the level of access which can be Administrator, Operator or Technician, the rights of
access to the menus and sub-menus are different.
Hereafter the menu tree with access levels required.
ARBORESCENCE ACCESS
MENU SUB-MENU FUNCTION OPERATOR ADMINISTRATOR TECHNICIAN
STARTUP
SHUTDOWN
CHANGE LOAD
QC
RESULT LJ GRAPHS
DETAIL
CHANGE
STATS
CALIBRATION
RESULT Calibrate
LOAD
DEL.UNSELECT
REPEATABILITY
DETAIL
RUN
NEXT SAMPLE
SAMPLE
DATE
RESULTS
DETAILS
PRIME ALL
DILUENT
PRIME
LYSE
PRIME
CLEANER
REAGENTS PRIME
WASTE
RESET
Cycle Counters
RESET
Reagents Log
SYSTEM INIT
EVENT LOGS
ERROR LOGS
BACKUP & RESTORE BACKUP

RESTORE
LAB PREFERENCES
UNITS
SERVICE CBC TRHESHOLDS
AND FLAGS
LAB PARAMETERS DIFF TRHESHOLDS
SETTINGS AND FLAGS
REFERENCE RANGES
CALIBRATION
FACTORS
DATE AND TIME
AUTOMATIC CYCLES
PRINTER PRINTER SETTINGS

PRINTERS
MANAGEMENT
COMMUNICATON
COMMUNICATION
SETUP
Add
USERS
Change Password
MANAGEMENT
Remove
SOFTWARE UPDATE
FLUIDIC CONTROL
BLEACH CLEANING
DRAIN BATHS
BACKFLUSH
APERTURES
BACKFLUSH OPT.
BENCH
NEEDLE
DISMANTLING
CHECK ROCKER
PARK MOTORS
RINSE
CLEAN
DRAIN FOR PACK UP
SYRINGE GREASING
DRAIN FLOW CELL
EV1
EV2
EV3
EV4
EV5
TROUBLESHOOTING EV6
EV7
EV8
EV9
EV10
EV11
EV12
CHECK
EV13
SENSORS/VALVES
EV14
EV15
EV16
EV17
EV18
ALL EV ON
EV CHASER
SET HGB LED OFF
SET CO ON
SET OPT LED ON
TEST VACUUM
PUMP ON
CHECK SYRINGE
CHECK NEEDLE
ADJ. LED
ADJ. OB
ADJUST DIF CHECK OB
ADJ.WBC
CHECK WBC
WBC ADJUST
TECHNICIAN
WBC CHECK
RBC ADJUST
ADJUST OTHERS
RBC CHECK
HGB ADJUST
PRESSURE ADJUST
SYSTEM CONFIG
LOG OUT

Chapter 6 Technology
6.1.1 Detection Principle WBC, RBC, PLT Counting

The counting as well as the discrimination of the cellular elements of the blood sample is based on The Coulter
Principle.
This technic is based on the modification of the impedance of a calibrated aperture soaking in an electrolyte
and going through a constant current delivered by two electrodes located on both sides of the aperture.
A vacuum applied on a side of the aperture allows the cells passage. Cells oppose their physical volume to the
current passage. A voltage impulse is registered at the electrodes terminal. The height of this impulse is
proportional to the cell volume.

6.1.2 Five-part diff measurement.

The principle of this technology called is based on the introduction of the sample solution flow in the flow
cell with low pressure and the introduction of the diluent sheath with a high pressure. Thus, the diluent
sheath drives the sample flow straight across the cuvette through the detection area.
The main advantages are:
 High level of reliability of the optical adjustment.

 Only two measurement axes for five parameters.
 High resolution matrix.
 Low level of contamination between two measurements.
 Low Reagents consumption
Hydraulic connection
Optical Flow Cell
Flow Cell holder
Nozzle holder
Hydraulic connection
4 White blood cells

Absorbance detection
Transmitted Incident Light

LED Light flow Flow
Diffraction
detection
Absorbance
Eosinophils Neutrophils
Lymphocytes
AGAPPE DIAGNOSTICS LTD Monocytes
Diffraction
YOUR BEST PARTNER IN DIAGNOSTICS Basophils 29
6.2 Hemoglobin Measurement

The hemoglobin measurement is directly done in the WBC chamber, by spectrophotometry ( = 545 nm).
Hemoglobin is detected by formation of oxyhemoglobin.
HGB blank check is performed at each STARTUP cycle.
HGB Blank measurement is done also at the beginning of each analysis cycle.
By comparison of the two values, it is possible to follow potential evolution of the blank value in order to warn
the user if necessary.

6.3 Leukocyte Analysis
The White Blood Cell Total Count is obtained by Impedance metric in the WBC counting chamber; the
other ten parameters are obtained by flow cytometry measurement.
Parameter Associated Pathologies

Leukocytosis when WBC>WBC h
WBC White Blood Cells Total count
Leucopenia when WBC<WBC l
LYM% Lymphocytes expressed in percentage Lymphocytosis when LYM # > LYM # h
LYM# Lymphocytes expressed in absolute value Lymphopenia when LYM # < LYM # l
MON% Monocytes cells expressed in percentage
Monocytosis when MON > MON h (% & #)
MON# Monocytes cells expressed in absolute value
Neutrophilia when NEU % > GRA % h
NEU% Neutrophils expressed in percentage
Neutropenia when NEU % < GRA % l
Neutrophilia when GRA # > GRA # h
NEU# Neutrophils expressed in absolute value
Neutropenia when GRA # < GRA # l
EOS% Eosinophils expressed in percentage
Eosinophila when EOS > EOS h (% & #)
EOS# Eosinophils cells expressed in absolute value
BAS% Basophils expressed in percentage
Basophilia when BAS > BAS h (%&/or #)
BAS# Basophils expressed in absolute value
IMM% Immature cells expressed in percentage
Immature Cells when IMM> IMM h (%&/or #)
IMM# Immature cells expressed in absolute value
ALY% Atypical lymph. cells expressed in percentage
Atypical Lymphocytes when ALY> ALY h(%&/or #)
ALY# Atypical lymph. expressed in absolute value

WBC Total Count calculation is based on the pulses distribution curve after the action of the lytic reagent.
The lyse reagent destroys the RBC and their stromas and acts on the cell cytoplasmic walls for better
discrimination into the optic measurement.
L1 L5
 WBC histogram is displayed after pressing on DIF Plot on the result screen.
 L1 and L5 thresholds are displayed on curve in full vertical blue color line.
NOTE: first peak on the left side of the histogram represents the lymphocytes cells, the other one located
on the right side represents all the others WBC populations.
WBC 5 differential absolute values and percentages are obtained by optic measurement.
The measured pulses on the two optical channels are displayed on DIF Plot ALL (Y axis) and FSC (X axis).
Each dot on the DIF Plot represents the height in ALL and FSC of each pulse
Lymphocytes (as well as atypical lymphocytes) population

is colored in PINK
Monocytes population is colored in BLUE
Neutrophils population is colored in GREEN (
Eosinophils population is colored in YELLOW (#FFFF00)
Basophils population is colored in ORANGE
Immature cells population is colored in RED
6.4 Erythrocyte Analysis

The erythrocyte analysis is done by impedance metric in RBC counting chamber. Seven parameters are
obtained:
Parameters Associated Pathologies

RBC Red Blood Cells Erythrocytosis RBC > RBC h
HGB Hemoglobin Anemia HGB < HGB b
HCT Hematocrit
MCV Mean Corpuscular Volume Microcytosis VMC < VMC b
Macrocytosis VMC > VMC h
MCH Mean Corpuscular Hemoglobin
MCHC Mean Corpuscular Hemoglobin Concentration Hypochromia MCHC < MCHC b
Cold Agglutinin MCHC > MCHC h
RDW-CV Red blood cells Distribution Width (CV) Anisocytosis RDW-CV > RDW-CV h
RDW-SD Red blood cells Distribution Width (SD) Anisocytosis RDW-SD > RDW-SD h
RBC calculation is based on the pulses distribution curve.
R1 R2
 RBC histogram is displayed on result screen.

 R1 and R2 thresholds are displayed on curve in full vertical blue color line.
 Hematocrit (HCT) is measured by integration of the volume of the red blood cells which flow in the
RBC counting chamber aperture.
HCT = MCV x 10
RBC

 Mean Corpuscular Volume (MCV) is obtained by calculation, following the formula:
MCV = HCT x 10
RBC
 The RBC distribution curve analysis allows the measurement of RDW which is an expression
of the standard deviation compared to MCV. This parameter evaluates the RBC anisocytosis.
RDW = k x SD
MCV
 Mean Corpuscular Hemoglobin (MCH) calculation is obtained from HGB and RBC by the following
formula
MCH = HGB x 10
RBC
 Mean Corpuscular Hemoglobin Concentration (MCHC) is made from HGB and HCT by the formula
below:
MCHC = HGB 100
HCT
 RDW-SD is an actual measure of size. It is derived by finding the width at the 20% height
of the distribution histogram.
 RDW-CV is determined by taking the standard deviation of RDW-SD and the mean
corpuscular volume (MCV) number.
6.5 Platelet Analysis
Platelet analysis is made by impedance metric in the RBC counting chamber.

Four parameters are obtained:

Parameters Pathologies
PLT Platelets Thrombopenia PLT<PLT b
Thrombocytosis PLT>PLT h
MPV Mean Platelet Volume Giant platelets MPV> MPV h
Small platelets MPV<MPV l
PCT Thrombocrit
PDW Platelet Distribution Width
PLCR Platelet Large Cell Ratio
PLT calculation is based on the pulses distribution curve.
CP1 CP2
P CP3
 PLT histogram is displayed on the result screen.

 Only P threshold is displayed in full vertical line using Blue as standard color.
 Thrombocrit (PCT) is made from PLT and MPV by formula below:
PCT = PLT x MPV
10000
 Platelet Large Cell Ratio (PLCR) indicates the percentage of large platelets with a volume >12 fL. Aside
from the two flexible discriminators which delimit the volume distribution curve, there is additionally
a fixed discriminator at 12 fL (marked in red on below picture). The PLCR is the percentage of cells
higher than 12fL regarding the whole platelets count.


6.6 Alarms
MISPACOUNTPLUS manages different alarms. These alarms allow the user to be alerted if there is a mistake
which can affect the quality of the results. These alarms appear on screen at the right of the result.
In presence of one or more alarms, it is recommended to check the result by a

conventional measure or on blood smear.
NOTE: Most of these alarms can be adjusted by the user.

6.6.1 General Flags
Each parameter value is accompanied with specific information per the automaton characteristics.
XXXX+: Over range on linearity high limit.

XXXX!: Suspicious result. This occurs when a morphological alarm has been triggered (please see chapters
below) or when an associated parameter might be suspected (For instance, if WBC if flagged with +, LYM,
MON, NEU, EOS and BAS will be flagged with +)
++++ : Higher than the reportable limits. WBC, RBC, PLT, HCT, HGB.
----: Invalid result
XXXX*: Rejected result
h: results higher than normal value.
L: results lower than normal value.
H: results higher than panic value.
L: results lower than panic value.
NOTE: Whenever one of the following symbol is triggered (*, -, +, ++++) display tagged result in bold (flag
included)
Whenever one of the following flag is triggered (L, l, h, H) display tagged result normally and flagged
in bold.
Whenever “!” is triggered display afferent result in italic (flag included)
Whenever one of the following flag is triggered (L, l, h, H) display tagged result normally and flagged
in bold.
Change color of all flagged result to a specific color in accordance to the UI specifications (flag
included):
 Orange (l, h) is used as standard color

 Red (L, H) is used as standard color
When result is flagged with: *, +, ++++,!, the parameter printout on table view is the parameter
value followed or replaced (++++) by the appropriate symbol
When result is flagged with L,l, h or H, the parameter printout on table view is in reverse video
only

6.6.2 Leukocytes Flags

Extraction of distribution curve in height of pulses (after initial pulse width based cut-off).
L1 L5
Two editable thresholds L1, L5 enable to discriminate the resistive count flags.
MORPHOLOGICAL ALARMS
On Curve
Trigger Condition Alarm Short version Alarm Flags enlarged
Determination of the population defined from channel CL1 Review on Smear:

to CL1-2. Small lymphocytes
The alarm is raised if this population is higher than an L1 Probable Incomplete Erytrolysis
absolute limit AND a percentage of the lymphocytes Platelet Aggregates
population. Erythroblats
Determination of the population defined by CL5 threshold
and curve ending. Review on Smear:
L5
The alarm is raised if this population is higher than an Immature Cells
absolute limit AND a percentage of the WBC population.

The measured pulses on the two optical channels are displayed on DIF Plot ALL (Y axis) Vs FSC (X axis).
Definition of height zones enable to discriminate optic count flags.
On DIF Plot
Presence of cells in the zone in Review on Smear:
regard of the total number of N1 Debris or Small Cells
leucocytes in the DIF Plot. Platelet Aggregates
Review on Smear:
Presence of cells in the zone in
Probable Incomplete Erytrolysis
regard of the number of N2
Platelet Aggregates
lymphocytes.
Erythroblasts
Presence of Immature Cells (from Review the DIF
the mono or polynucleated cells IMM Review on Smear:
line) Immature Cells
Review the DIF
Presence of atypical lymphocytes ALY Review on Smear:
Atypical Lymphocytes
Review the DIF
Presence of atypical lymphocytes
RL Review on Smear:
or basophiles.
Right Lymphocytes
Presence of basophiles, small
Review the DIF
neutrophils (without granulations HL
High Lymphocytes
or few segmented), band cells.
Presence of small neutrophils
(without granulations or few Review the DIF
NL
segmented), band cells or hyper Low Neutrophils
basophil monocytes.
Presence of giant neutrophils,
hyper segmented neutrophils, Review the DIF
NH
eosinophils with few granulations High Neutrophils
or damaged eosinophils.

6.6.3 Erythrocyte and HGB Flags

Extraction of distribution curve in height of pulses (after initial pulse width based cut-off).
Two (2) editable thresholds R1 and R2 enable to discriminate between microcytes, normocytes and
macrocytes. CR1 CR2
100%
68.26% of total
RDW distribution area
RDW-SD
20%
MCV
 MCV is measured on the whole RBC acquisition.
 HCT parameter is calculated from MCV and RBC parameters.
 MCHC parameter is calculated from HGB and HCT parameters.
 MCH parameter is calculated from HGB and RBC parameters.
 RDW parameter is calculated on curve distribution (defined as the CV of 68.26% of total distribution
area)
 RDW-SD parameter is calculated on curve distribution (defined as curve width at 20% of peak)
On The Curve
Determination of the population defined
from channel 0 to CR1.
The alarm is raised if this population is R1 Microcytes
higher than an absolute limit OR a
percentage of the RBC population.
by CR2 threshold and curve ending.
The alarm is raised if this population is R2 Macrocytes
percentage of the RBC population.

6.6.4 Platelet Flags

Extraction of distribution curve in height of pulses (after initial pulse width based cut-off), below an editable
threshold P that discriminates between PLT and RBC (sample dependent).
On The Curve
from channel 0 to CP1.The alarm is
raised if this population is higher than an P1 Platelet Debris
absolute limit OR a percentage of the
PLT population.

by CP2 threshold and P.
The alarm is raised if this population is P2 Schizocytes
percentage of the PLT population.
by CP3 threshold and over a CP3-2 width
at the right of CP3.
P3 Microcytes
The alarm is raised if this population is
higher than an absolute limit AND a
percentage of the PLT population.
NOTE: Only P threshold is displayed on histogram.

 MPV parameter is calculated on curve distribution (defined as curve width at 20% of peak)
 PCT parameter is calculated from PLT and MPV parameters.
 P-LCR is calculated as the percentage of platelets with a volume higher than 12fL, i.e. those
appearing between 12fL and P.
 P-LCR is significantly decreased in patients with thrombocytosis while it is increased in
thrombocytopenia. In patients with high counts, P-LCR is significantly decreased in reactive
thrombocytosis than neoplastic thrombocytosis. P-LCR is more likely higher in destructive
thrombocytopenia than hypoproliferative thrombocytopenia. P-LCR is inversely related to platelet
count and directly related to PDW and MPV.

6.6.5 System Alerts

6.6.5.1 INS-T / Instrument Temperature Out Of Range
Means that the Instrument temperature is lower than 17°C or higher than 33°C and the result could be
affected.
6.6.5.2 WBC BAL / WBC Balance

Unbalanced counting between the resistive and the optical measurement on the WBC Channel. This may be
explained by a clog on the WBC aperture, a clog on the WBC flow cell or that the stop cock has been left as
closed.
6.6.5.3 WBC-CL / WBC Aperture Clog

Clog detected during WBC measurement.
6.6.5.4 RBC-CL / RBC Aperture Clog

Clog detected during RBC or PLT measurement.
6.6.5.5 O-DF / Optical Default

Unstable LED light low measurement during WBC DIF measurement. This may come from bubble or
unexpected phenomenon during WBC DIF measurement.
6.6.5.6 SU-F / Startup Failed

Background limits or HGB blank are out of range.
6.6.5.7 QC Not Done / QC Not Done During The Day

While no QC are done during the day. This system alert is set to every analysis until a QC sample is run.
6.6.5.8 QC Failed / Last Run QC Failed

The last QC result is out of the lot limits. This system alert is set to every analysis until a correct QC is done.

6.7 Hydraulic Description

The fluidic part is located on the right side of the instrument directly accessible after opening of the fluidic
door.
The fluidic part consists of the six elements listed below:
1. Sampling module
2. Syringe module
3. Syringe valve module
4. Counting module
5. Counting valve module
6. Optic Bench
1
3

6.7.1 Sampling module

This module (patented) allows the blood sampling and dispensing in counting chambers (WBC and RBC) to
perform appropriate dilutions.
It supports the needle and its rinse block assembly called rinse head.
The needle and its rinse head can be removed completely without tool.
The O-ring of the needle located inside the rinsing head can also be removed without tool.
The maintenance of these parts is very easy to perform please refer to the maintenance chapter to perform it
as describes.
6.7.2 syringe module

This module allows performing the aspiration/dispense of the reagents, blood’s drawing, vacuum and positive
pressure.
6.7.3 Syringe valve module

This module supports the valves allowing opening or closing the reagent and waste syringes hydraulic circuit
as well as the optic hydraulic circuit.
6.7.4 Counting module

This module allows to count the WBC and RBC/PLT and to measure the HGB.
6.7.5 Optic Bench

This module allows discriminating the 5 parts diff for WBC.
6.8 Software
MISPACOUNTPLUS software runs using the Linux operating system. The software is embedded on a Q7 CPU
board.
This board is equipped with an Intel ATOM CPU (32 bits), RAM memory and flash where software and data
are stored.
6.8.1 Graphical User Interface

Common keys present in all screens:
: Allows coming back to the previous screen in the menu tree.
: Allows to opens a contextual menu for actions linked to the current menu (save, delete,
print, sent…).
An exit button allows to close the tools window and return to current menu.
: Allows coming back to the MENU display where ever you are in the arborescence.

: Display of current state of the instrument:
 Instrument Model
 Operation System Version
 Software version
 FPGA / µblaze versions
 Algorithm (dynamic clustering version)
 Cycles Modules
 Fluidic Module: N/A
 Instrument’s serial Number
 User - Login level
 IP address
6.8.2 Windows
MispaCountPlus displays few types of window to communicate with the operator in case of needs.
1. Confirmation message.
2. Information Message

Chapter 7 Specifications
7.1 Analytical Specifications

7.1.1 Linearity
Linearity of WBC, RBC, HGB, HCT and PLT will be verified using linearity kits.
Measuring
Measurand Units* Limit Operating Range
Range
WBC 103/µL 0.2 – 100 ± 0,4 or ± 4% 0-150
0.02 – 8.0 ± 0.05 or ± 3%

RBC 106/µL 0-15
8.0 – 15 ± 0.10 or ± 4%
HGB g/dL 0.2 – 24 ± 0.2 or ± 2% 0-25
HCT % 5 – 70 ± 2 or ± 3% 0-80
MCV fL 50-150 ±2.5 or ±3.0% 50 - 150
PLT 103/µL 10 – 2000 ± 10 or ± 10% 0 - 4000
RDW-CV % 10 – 40 ± 1.5 or ± 10% 0 – 70
RDW-SD fL 15 – 150 ± 6.5 or ± 10% 0 – 220
MPV fL 5 – 25 ± 1 or ± 10% 0 – 25
MCH pg N/A N/A 0 – 99.9
MCHC g/dL N/A N/A 0 – 99.9
PCT % N/A N/A 0 – 9.999
PDW % N/A N/A 0 – 99.9
PLCR % N/A N/A 0 - 100

LYM, MONO,
NEU, EOS, BASO, 103/µL 0-100 N/A 0-150
ALY, IMM #
LYM, MONO,
NEU, EOS, BASO, 103/µL 0-100 N/A 0-100
ALY, IMM %

7.1.2 Background
Maximum background counts during STARTUP cycle
Measurand* Background Concentration Limits
WBC / DIFF < 0.2 103/µL
RBC < 0.02 106/µL
HGB < 0.2 g/dL
PLT < 10 103/µL
*Results are expressed in

Standard (US) units
7.1.3 Precision
Short term (within run) imprecision will be tested by assaying the same normal whole blood (collected in
K2EDTA) specimen consecutively 20 times.
Repeatability Limits
Measurand* Ranges Tested
Whole blood (%CV)
WBC (103/µL) >6.0 < 2.5
RBC (106/µL) >3.5 < 2.0
HGB (g/dL) >11 < 1.5
MCV (fL) >80 < 1.0
HCT (%) >35 < 2.0
RDW-CV >12 < 4.0
RDW-SD >40 < 4.0
PLT(103/µL) >200 <5.0
MPV (fL) >8 <3.0
Lymphocyte (%) >15 < 5.0
Monocyte (%) >5.0 < 10
Neutrophil (%) >40 < 3.0
Eosinophil (%) >5.0 < 10
Basophil (%) >1.0 < 40

*Results are expressed in Standard (US) units
7.1.4 Carry-Over
The following table shows carryover percent for WBC, RBC, HGB and PLT. Carryover will be determined by
running linearity kit specimens with high target values of WBC, RBC, HGB and PLT. Each specimen will be run
in triplicate followed by three blank runs. Carryover is calculated and expressed as a percentage using the
following formula:
Measurand Target Values % Carryover (95%

(units*) Low Target Values High Target Value Confidence Limit)
WBC /
> 0 < 0.2 >15 <1%
DIFF(103/µL)
RBC(106/µL) > 0 < 0.02 >6 <1%
HGB(g/dL) > 0 < 0.2 >20 <1%
PLT(103/µL) > 0 < 10 >300 <2%

7.1.5 Correlation
Specimens from normal blood donors and specimens from patients identified with normal and abnormal
hematology results will be run in duplicate on test systems and comparative Systems using same
measurement technique.
All results have no alarm or flag.
Measurand* Range Tested r-value Comparability
0.2-100 ≥0.95 N/A
WBC (103/µL) 0.2 to <2.00 N/A ±10%
2.00 to 100 N/A ±5%
RBC (106/µL) 0.02 – 7.0 ≥0.95 ±2.5%
HGB (g/dL) 0.2 -22 ≥0.95 ±2.5%
HCT (%) 15 – 60 ≥0.90 N/A
MCV (fL) 55-120 ≥0.90 ±3.0%
RDW-CV (%) >12 ≥0.60 N/A
RDW-SD ≥ 35 ≥0.60 N/A
PLT (103/µL) 10-1500 ≥0.95 N/A
MPV (fL) >7 ≥0.90 N/A
Lymphocyte (%) >15 ≥0.95 N/A
Monocyte (%) >5.0 ≥0.95 N/A
Neutrophil (%) >40 ≥0.95 N/A
Eosinophil (%) >5.0 ≥0.90 N/A
Basophil (%) >1.0 ≥0.15 N/A

7.2 Units
MISPACOUNTPLUS is multi units for hematology result.
US
Parameters SI SI MOD JAPANESE
(Standard)
WBC XXX.X XXX.X XXX.X XXXX.

103/µL 109/L 109/L 102/µL
LYM, MONO, NEU, EOS, BASO, ALY, IMM # XXX.X XXX.X XXX.X XXX
103/µL 109/L 109/L 102/µL
LYM, MONO, NEU, EOS, BASO, ALY, IMM % XX.X XX.X XX.X XX.X
% % % %
RBC XX.XX XX.XX XX.XX XXXX
106/µL 1012/L 1012/L 104/µL
HGB XX.X XXX XX.XX XX.X
g/dL g/L mmol/L g/dL
HCT XX.X X.XXX X.XXX XX.X
% L/L L/L %
MCV XXX.X XXX.X XXX.X XXX.X
fL fL fL fL
MCH XX.X XX.X X.XX XX.X
pg pg fmol pg
MCHC XX.X XXX XX.XX XX.X
g/dL g/L mmol/L g/dL
RDW CV XX.X XX.X XX.X XX.X
% % % %
RDW SD XXX,X XXX,X XXX,X XXX,X
fl fl fl fl
PLT XXXX XXXX XXXX
XXX.X 104/µL
103/µL 109/L 109/L
MPV XX.X XX.X XX.X XX.X
fL fL fL fL
PCT X.XXX X.XXX X.XXX X.XXX
% cL/L cL/L %
PDW XX.X XX.X XX.X XX.X
% % % %
P-LCR XX.X XX.X XX.X XX.X
% % % %
 L: Liter

µ: micro (10-6)
 g: gram
 dl: deciliter
 f : femto (10-15)
 mmol : millimole (10-3 mole)
 mol: mole
 SI MOD: SI modified (mole instead of g)
 JAPANESE: Commonly used in Japan

7.3 Reagent consumption

Consumption in ml based on measurement by weight.
CYCLES Type DILUENT LYSE CLEANER

WB CBC Analysis Analysis 15,14 0,92 0,48
WB DIF Analysis Analysis 17,40 1,00 0,51
PD CBC Analysis Analysis 14,89 0,92 0,48
PD DIF Analysis Analysis 17,19 1,02 0,49
Dil Dispense Analysis 0,30 0,00 0,00
Fluidics Control Daily Use 15,04 0,11 0,29
Pre-Startup Daily Use 63,59 2,71 1,48
Startup Daily Use Pre-Startup + from 1 to 3 WB DIF cycles
Shutdown Daily Use 0,00 0,47 32,24
Automatic Rinse Daily Use 16,19 1,09 0,53
Bleach Clean 61,69 1,27 9,40
Back flush Clean 11,72 0,10 0,71
Backflush Optic Clean 5,51 0,19 0,00
Cleaning Clean 17,08 0,42 15,02
Prime ALL Prime 39,10 6,50 4,90
Prime Diluent Prime 39,13 0,42 0,00
Prime Lyse Prime 5,71 6,47 0,00
Prime Cleaner Prime 0,00 0,00 5,05
Rinse Chambers Rinse 11,70 0,10 0,00
Rinse WBC BAL Rinse 11,62 0,89 0,00
Prime_Wait Rinse 16,19 1,09 0,53
Latex RBC Adjust. 7,55 0,00 0,39
Latex WBC Adjust. 13,16 1,01 0,38
Adjust OB Adjust. 17,51 1,02 0,00
Adjust OB LED Adjust. 5,90 0,05 0,00
Check OB / ADJ WBC / CHECK
Adjust. 16,84 1,11 0,00
WBC
Adjust HB Adjust. 11,70 0,10 0,00
Drain For Pack Up Drain 61,69 1,27 9,40
Drain Flow Cell Drain 5,42 0,10 0,00
7.4 Physical Specifications

General:
Ambient temperature: from 18 to 32°C
Relative Humidity: 80% maximum at 32°C
Storage temperature: -10 to 50°C
If MISPACOUNTPLUS has been stored at a temperature less than 10°C, it must stay at
room temperature during 24 hours before switching it on.

If the room temperature moves more than 10°C during the working day,
MISPACOUNTPLUS must be calibrated.
7.4.1 Instrument Specifications
Here below the physical specifications of the instrument:
- Dimensions: Height: 405 mm (approx.) * Width: 270 mm (approx.) * Depth: 430 mm (approx.)
- Weight: 12kg (approx.)
- Power supply Input: 100-240VAC 50-60Hz
- Power supply Output: 24V – 6.75A
- Electric consumption: 160W
- Screen: LCD Touch-screen 8.4 inch (800*600)
- Memory capacity: 35000 Files (Demographics, results and histograms)
QC: 12 lots (100 results per lot)
- Connection: 5 USB ports/ Ethernet-RJ45/ Serial LIS-SUB D9, PC connection/Serial Port
7.4.2 Power Supply Block

MISPACOUNTPLUS is equipped with an external Power Supply block
 Dimensions: Height: 31 mm (approx.)

 Width: 58,5 mm (approx.)
 Depth: 132 mm (approx.)
 Weight: 0,35 kg (approx.)
 Power supply Input: 100 to 240V AC - 1,5A, 50-60Hz

7.5 Reagents Specifications

All the reagents must be stored at temperature stated on their labels.
If the reagent has been stored at a temperature less than 10°C, it must stay at room
temperature during 24 hours before use.
7.5.1 MISPACOUNTPLUS Diluent (DIL-5D)

Shelf life: 3-years closed shelf life and 60-days open shelf life
Application: MISPACOUNTPLUS Diluent is used to carry out the necessary dilutions for the measurement
performed by MISPACOUNTPLUS
Description: Clear and odorless aqueous solution.
Storage: Between 2°C and 30°C, until the expiration date printed on the container label.
Precautions: Avoid contact of the preparation with skin and eyes. Wear protective gloves and glasses. For
detailed precautions please refer to relevant Material Safety Data Sheet.
7.5.2 MISPACOUNTPLUS Cyanide Free Lytic Solution (Lyse-5D)

Application: MISPACOUNTPLUS Cyanide Free Lytic solution is used in Lyse of the red blood cells and the
leukocytes differentiation during the measurement performed on MISPACOUNTPLUS.
Storage: At temperature between 2°C and 25°C, until the expiration date printed on the bottle label.
7.5.3 MISPACOUNTPLUS Enzymatic Cleaning Solution (Cleaner)

Enzymatic Cleaning Solution is used to carry out the cleaning of the measurement
system and hydraulic circuit.
Description: Odourless, blue colour.
Storage: At temperature between 2°C and 30°C, until the expiry date printed on the bottle label.
First emergency care:

After exposure by respiratory tract: Supply fresh air, consult doctor in case of
symptoms.
After skin contamination: Wash off with large amount of water. Take off
contaminated clothing.
After contamination of eyes: Rinse with large amount of water for at least 15
minutes. Consult a doctor.
After consumption: Give the sufferer a lot of water to drink.
If the sufferer feels unwell, consult a doctor / ambulance.
For detailed first aid measures please refer to relevant Material Safety Data Sheet.

7.3 Reagent consumption

Consumption in ml based on measurement by weight.

7.6 Analytical Limitations

MAINTENANCE Recommendations: Please respect the maintenance procedure and the quality control
procedure. Otherwise, results can be affected.
GENERALITIES: Some abnormal samples may give incorrect results by automated cell counting methods.
The following table shows examples of specific specimens that could cause errors.
Each result for a new patient out of lab linearity limits or with an alarm must be
checked with a conventional method or checked with blood smear.
7.6.1 Interferences
Parameter Specimen Possible Indication of Error

Cold Agglutinin (+) ↑MCV, ↓HCT, red cell clumping on smear NRBC
Nucleated RBC (+) on smear
WBC Cryoglobulins (+)
Platelet aggregation (+) Platelet aggregates on smear
Erythroblastosis (+) Erythroblasts on smear
Cold Agglutinin (-) ↑MCV, ↓HCT, red cell clumping on smear
Severe Microcytosis (-) Elevation of WBC
RBC
Fragmented RBC (-)
Leukocytosis (>100,000/µL) (+)
Leucocytoses(>100,000/µL) (+) Elevation of WBC
HGB Lipémie (+) ↑MCHC, “milky” appearance of plasma
Abnormal Protein (+) ↑MCHC, Lysed Hgb/WBC sample turns cloudy
Cold Agglutinin (-) ↑MCV, ↓HCT, red cell clumping on smear
Leukocytosis(>100,000/µL) (+) Elevation of WBC
HCT
Abnormal Red Cell Fragility (?)
Spherocytosis (?) ↓MCV, spherocytes on smear
Pseudothrombocytopenia (-) Platelet Satellitism on smear
Platelet Aggregation (-) Platelet Aggregates on smear
PLT Increased Microcytosis (+) ↓MCV
Megalocytic Platelets (-)
(+): Instrument count is affected by an increase in the result.

(-): Instrument count is affected by a decrease in the result.
(?): Instrument count is affected by either an increase or decrease in the result which is sample dependent.

Chapter 9 SERVICE
9.1 Introduction
From the main menu, select , the following screen is displayed.
No Available in this screen:
9.2 SYSTEM INIT

This option allows performing a mechanic initialization of all the modules.
NOTE: Can be used whether the instrument is switched on with the fluidic door opened and due to that,
did not performed the automatic mechanic initialization.

9.3 LOGS
Log depth (EVENT+ERROR) is set to 500 entries.
When the number or EVENTS+ERRORS is higher than 500, older messages are removed from the database.
9.3.1 Event logs

This option allows displaying the EVENT logs of the instrument.
EVENTS are the most important actions performed or requested by the instrument’s user.
Among EVENTS lists, the user can find: WASTE IS RESET, WASTE SET AS OPEN DRAIN, PRIME ALL CYCLE
REQUESTED, STARTUP STARTS, ….
For each EVENT, the Date, Time, Operator, Event Type and Event description is recorded.
Available in this screen:
9.4 ERROR LOGS

This option allows to display the error logs of the instrument.
Each occurrence of ERROR message window will be recorded in this file.
Among ERRORS lists, the user can find: PLEASE REPLACE DILUENT, PLEASE REPLACE DILUENT, ….
For each ERROR, the Date, Time, Operator, Event Type and Event description is recorded.
Available in this screen:

9.5 BACKUP & RESTORE

This option allows to backup and/or to restore the patients result (.csv format) the set up and adjustments.
9.5.1 BACKUP
In BACKUP field, check the options you want backup “Data and Setup” and/or “.csv file” then select
.
The following window is displayed.
Connect an USB drive then press to confirm the backup.
9.5.2 RESTORE
In restore field, press on , the following window is displayed.

Connect an USB drive then press to confirm the restoration.
NOTE: It is recommended to perform backup regularly because in case of trouble it would be the easiest
way to restore all data in the state of the last backup save.
9.6 SETTINGS
This menu is dedicated to set all the instrument with different options like printer, communication, limits,
flags, etc…
Select from menu, the following screen is displayed.
9.6.1 LAB PARAMETERS

This menu allows to access to set-up options as described below.

9.6.1.1 LAB PREFERENCE

 DIF DISPLAY FORMAT

Select the option desired for the DIFF result format among:
 Auto increment
Check in box if you want auto-increment of SID from start number specified.
 QC
Check in the box if you want QC alert displayed and printed on patient files (QC Not Done and QC Failed)
 RUO
Check in the box if you to see displayed the RUO parameters.

Note: See below the difference of display between with and without RUO parameters.

9.6.1.2 UNITS
This screen gives the possibility to the user to choose the units among the following list
9.6.1.2.1 US Format & units
9.6.1.2.2 SI Format & units

9.6.1.2.3 SI MODE Format & units:
9.6.1.2.4 Japanese Format & units:
 Select the model of unit you want use then press to valid, the following window is displayed.
 press to save change or to cancel.

9.6.1.3 CBC THRESHOLDS AND FLAGS

This menu allows to modify the thresholds and Flags for each CBC parameters.
No Available in this screen
Thresholds modification can affect the quality of the results or can affect the alarm
detection. We recommend to modify these values only after training.
9.6.1.4 DIF THRESHOLDS AND FLAGS

This menu allows to modify the thresholds and Flags for each DIF parameters.
Thresholds modification can affect the quality of the results or can affect the alarm
detection. We recommend to modify these values only after training.
NOTE: In both CBC & DIF thresholds and flags menus, the button allows returning to the
original values.

9.6.1.5 REFERENCE RANGES

This menu allows modifying the existing values of normal and panic limits values for all parameters.
1. allows displaying the CBC

parameters.
2. allows displaying the DIF
parameters.
3. allows coming back to the default
values.
Once modifications are done, press to validate or to exit without any modification.
Following window is displayed

9.6.1.6 Calibration Factors

This menu allows modifying the calibration factors manually without to redo the complete calibration.
The modification of these factors without running a calibration blood could affect the
quality of the result.
If the calibration factors are manually modified, the calibration information in the
calibration screen will be flagged with “MODIFIED” and previous calibration results are
modified
If the calibration factors are manually modified, the following prompt is displayed to
inform the biologist that the calibration results will be removed.

9.6.2 DATE/TIME
This menu is dedicated to modify the Date /Time Setting

 To change the Date, select the field among day/month/year and change the value using the arrows or
enter it manually using the displayed numeric keyboard.
 To change the date format, press on the arrow and choose the desired format.
D for day
M for month
Y for year
 To change the time, select the minute or hour field and change the value using the arrows or enter it
manually using the displayed numeric keyboard.
 To change the time format, select the arrow and choose the desired format.
 Once modifications are done, press to validate or to exit without any modification.

9.6.3 AUTOMATIC CYCLES

This menu is dedicated to set the different automatic cycle.
 To enable automatic power up setting, Select Enable and choose the time and date for the automatic
power up.
 To modify the Auto clean frequency, adjusted by default to be done every 100 analyses, the value can be
set from 10 to 5000.
 If you want set an automatic shutdown, check in the option enable and enter the time chosen.

NOTE: Time is expressed in minutes and Shutdown frequency can be set from 30 to 720.
9.6.4 PRINTER
This menu is dedicated to set the different possible printing options.
9.6.4.1 PRINTER SETTINGS

This screen is dedicated to the printing option settings.
Select , the following screen is displayed.
 Report Headers: Allows to enter the Laboratory header using touch screen keyboard.
 Patient Report Options: Options selected will be printed on the patient report.
 Auto Print: Options selected will be automatically printed.
 Control Report Options: Allows to select LMG format, percentage or absolute value for all analysis
and not only for control.

 Printer Selection: Allows to select the printer and paper size.
 Once modifications are done, press to validate or to exit without any modification.
9.6.4.2 PRINTER MANAGEMENT
This screen is dedicated to the printer installation.
It must be used the first time the printer is connected to the instrument, following the instruction below.
 Connect the printer to an USB port located at the rear of the instrument.
 Switch ON the printer.
 Select , the following screen is displayed.
NOTE: It needs few seconds for the instrument to detect the printer as in the example below.
 Press on printer name’s field, the following window is displayed.

 Press , the following window is displayed.
 Select the field corresponding to the printer connected then press .
NOTE : As in the exemple above, if the field of the printer, connected and detected, is not present in the
list, choose pcl3 or pcl6 drivers depending from printer compatibility.
Generally: pcl3.ppd in dedicated to black & White printers and Pcl6.ppd for colors printers.
 Exit the menu pressing PREVIOUS, then go back and check that the printer is well installed.

Previously not installed
 Go back to and select the type of printer in Printer Selection field.
 Press to valid, the following window is displayed.
 Press to save changes the printer is installed.

9.6.5 Communication
This menu allows to setup the connection between MISPACOUNTPLUS and the Host Computer of the Lab.
Select from menu, the following screen id displayed.
 Communication field allows the user to select the type of communication required.
o COMMUNICATION FORMAT, at that time only CSV format (Coma Separated Values)
NOTE: MISPACOUNTPLUS can be connected to the host computer in two different ways:
1. Serial link (type RS232)
2. Ethernet
3. Select NONE if no communication is required.

 allows to configure the communication data accordingly to the host computer dat.
 Auto Transmit field provide the possibility to choose the data to be transmitted. Select the concerned
options.

9.6.6 USERS MANAGEMENT
This menu allows to add, to modify or to delete an operator and/or a biologist Login information.
Select from menu, the following window is displayed.
9.6.6.1 Add
Select to add a new operator or biologist and fill in the different fields.
9.6.6.2 Change Password

Select to change the password of the selected user and fill in the different fields.

9.6.6.3 Remove
Select to delete the selected user, the following window is displayed.
press to validate or to exit without modification.
Note: at least one Biologist id must stay in the list.

9.6.7 SOFTWARE UPDATE

This option allows to update the software when necessary.
Select , the following window is displayed.
 Connect to the front USB port, USB drive containing the software update (two files), then press ,
the following screen is displayed.
 Select to valid the software update, the following screen is displayed.
NOTE: Software upgrade can take few minutes, do not remove the USB drive or power off the instrument
until the following message.

9.7 TROUBLESHOOTING
This menu gives access to different fluidic options as well as mechanical checks.
Select , the following screen is displayed:
9.7.1 FLUIDICS CONTROL

option allows performing a complete control cycle of the mechanic and the fluidic of
the instrument in case of needs. A control bar indicates the progress of the cycle
NOTE: Control cycle allows to control mechanic, hydraulic and electric functions to completely reinitialize
the instrument when it is needed.
Example of need: In case of mechanical or hydraulic problem, immediately press on button,

MISPACOUNTPLUS will perform an emergency stop. After having identified and fixed the problem, it is
necessary to perform a Control cycle to reinitialize the instrument mechanically and hydraulically.
When control cycle is finished, the following window is displayed

9.7.2 BLEACH CLEANING

option allows performing a concentrate cleaning cycle using Sodium Hypo chloride
solution*
Hypo chloride solution*: The recommended concentration of Hypo chloride solution

is 12°.
NOTE: In normal conditions, it is recommended to perform bleach cycle one time a week for a daily
workload of 50 analyses. Bleach cycle can be performed also in case of permanent rejection for one or few
measured parameters. Follow the instruction below for bleaching.
1. Press on button, the following.
 Select to confirm, the following window is displayed.
2. Instrument begins to drain the counting chambers and the following window is displayed.
3. Open the fluidic door and put 4ml of 12° Hypo chloride solution in each counting chamber. Close the
fluidic door when bleach dispense is done, then press . The system will perform fluidic actions
cleaning the mains counting module parts like apertures, counting chambers and optic flow cell.

NOTE: On each counting bath, an Arrow indicates the volume of 4ml for bleach P-5.
The bleach cycle takes 15 minutes to be completed.
It is not possible to use the instrument during that time.
9.7.3 DRAIN BATHS
option allows draining the two counting chambers in case of needs.
The following window indicates the progress of the cycle.
NOTE: This option is principally used by field FSE in case of parts replacement which does not require a
complete instrument draining.
9.7.4 BACKFLUSH APERTURES
option allows to perform a backflush onto the apertures* in case of needs.
*backflush onto the apertures: The system push cleaner under pressure into the WBC and RBC apertures in
order to remove a potential clogging. It can be used in case of permanent rejection for one or few measured
parameters.

9.7.5 BACKFLUSH OPT.BENCH
option allows to perform a backflush onto optic flow cell* in case of needs.
*backflush onto optic flow cell*: The system push cleaner under pressure into the flow cell in order to
remove a potential clogging. It can be used in case of permanent rejection for optic measurement.
9.7.6 NEEDLE DISMANTLING
option allows moving automatically the sampling module to give the access to the
needle in case of needs (needle and/or rinse head O-ring replacement.
Hereafter the instruction to replace the needle and/or the rinse head O-ring.
1. Press , the system moves the Sampling module in “Needle Disassembling

position” and the following window is displayed.
2. Pull on the top of the needle to remove it from the needle carriage.
3. Pull on the rinsing head and remove it from the sampling module.

4. Pull up the needle to remove it from the rinsing head. Disconnect it from tubing and remove it
from the instrument.
5. If needed, replace the needle following the reverse instruction.
Wear rubber gloves and wash hands with a disinfectant after completion of work.
9.7.6.1 CHECK ROCKER
option allows controlling the functionality of the sampling module transversal axis; it
checks the motor and sensors.
9.7.7 PARK MOTORS

option allows to place the syringe pistons into the maximum high position.
NOTE: This option is Mainly used for instrument transportation.
9.7.8 RINSE
option allows the counting chambers rinsing, following tool bar indicates the progress of
the rinse cycle.
At the end of the rinse cycle the following window indicates the user that rinse cycle is completed.
9.7.9 CLEAN
option allows cleaning the apertures with cleaner. It performs three back flush, a
drain chambers and refill.
9.7.9 DRAIN FOR PACK UP
option allows performing a complete cleaning of the instrument. This procedure

includes cleaning MISPACOUNTPLUS with sodium hypochlorite, then rinsing with distilled water and drying.
NOTE: This option must be always used before a long term shut down.
9.7.10 SYRINGE GREASING
: This option allows moving the pistons down in order to perform the greasing.

NOTE: The piston greasing must be performed every 6 months, please proceed as describe in the
procedure below.
Operators must be trained before to perform the maintenance tasks. Due to moving
parts risk of injuries is present.
 Hereafter the instruction to perform the pistons greasing.
1. Press on and wait for the following information window.
2. Wear rubber glove on one hand and place a bit of silicon grease at the tip of the index.
3. Spread a thin film of grease on each piston excepted sampling piston.

NO GREASE ON SAMPLING
PISTON
NOTE: With a tool key type T20, turn the two bigger pistons (waste pistons) in order to spread
the grease all around the pistons.
9.7.11 CHECK SENSORS/VALVES
: This option is a control panel of all the sensors of the instrument.

 HOMES STATES
means the module is not at the

initialization position, home sensor is
not detected.
means the module is at the home

position, home sensor is detected.
 SWITCHS
means the start analysis trigger is

not activated.
means the fluidic door is closed.
 COUNT

 THERMAL
Value of vacuum/pressure measured in waste

chambers
Value of the vacuum generated by the rinse pump
Diluent Temperature (measured by diluent t° sensor)
Reagent temperature measured by heater t° sensor
Instrument temperature
Percentage of heating (from 0 to 100%)
 VALVES
To test each valve, press the dedicated

button.
To test all the valves, press ALL EV ON.
To test the valves one by one, press EV
CHASER.
 CHECK DEVICE

: This function allows switching ON/OFF optic LED.
: This function allows switching ON the apertures current.
: This function allows switching ON/OFF the Hemoglobin LED.
: This function allows controlling the vacuum.
: This function allows switching ON/OFF the rinse pump.
9.7.12 CHECK SYRINGE
option allows controlling the syringe module functionality, this function checks the
syringe motor and sensor.
9.7.13 CHECK NEEDLE
option allows controlling the functionality of the Y axis of the sampling module, this
function checks the motor and sensors.

9.7.15 Maintenance
The quality of the results and the reliability of MISPACOUNTPLUS are directly linked to the strict
respect of the maintenance hereafter described.
To perform the maintenance and the repair described in this Chapter, it is mandatory
to have received adequate training, to wear rubber gloves and wash hands with a
disinfectant after completion of work.
NOTE: This maintenance table is dedicated to the user and to FSE. It is established an average
workload of 50 daily patients. For bigger workload, please increase proportionally the frequency of
maintenance actions.
Needle
Motor
Pistons O-ring
Startup Shutdown Bleach screw
greasing replacemen greasing
t
U  
Daily

Weekly
Semi 
annually
Annually
 

Troubleshooting
9.7.16.1 Analytical problems
PARAMETERS PROBLEMS CONDITIONS SOLUTIONS

No results No HGB Check the HGB LED wires.
Check the electrode wires.
WBC No results HGB OK Perform a Cleaning Cycle and then a Bleach cycle if
unsuccessful.
Perform a Back flush and a Cleaning Cycle and then a
Bad
Bleach cycle if unsuccessful.
stability
Check if bubbles in WBC bath during the analysis cycle.
Check the electrode wires.
No HCT nor
No results Perform a Cleaning Cycle and then a Bleach cycle if
PLT
unsuccessful.
RBC Perform a Back flush and a Cleaning Cycle and then a
Bleach cycle if unsuccessful.
Bad
HCT & PLT too Check if bubbles in RBC bath during the analysis cycle.
stability
Check the level of bubbles in WBC bath during the first
dilution.
No results Check if the GHB LED is lighted on.
Check the level bubble flow in the WBC lath during the
Bad
run cycle.
HGB stability
Change Lyse reagent
---
Rejection Perform a new Start Up cycle.
*
9.7.16.2 Other problems
ORIGIN PROBLEMS SOLUTIONS

Check the rinsing needle block (presence of clots) and
Diluent leaks around the needle
clean it if necessary
during the run cycle
Check the rinse pump
Check the power supply connection wires.
Instrument No starting
Check the Power Supply block
Check the level of diluent and if the supply tubing is not
pinched.
All results bad
Verify if the diluent container is well located placed at the
same level than MISPACOUNTPLUS
Check the screen wires connection on CPU boardflat
No display
cable.
Check the paper.
Printer No printing
Check the electrical connection.

9.7.17 Troubleshooting Messages
This Chapter allows knowing what to do when a troubleshooting message appears on the screen.
In any case, if a problem is not solved, contact your distributor.
Messages Description Action/Troubleshooting
System detected Optical stop cock as closed. Open the stop cock if it’s closed.
Check that the optical bench isn’t fouled up.
System detected that temperature is < to Wait a moment, the heater will reach set
18°C or > to 36.5°C. points.
System detected a reagent temperature Wait a moment, the heater will reach set points
that is < to target – 2.5°C.
System detect a Reagent temperature Check the heater.

overflow. (Reagent temperature > 60°C). Reboot the automaton.
System detected an efficiency failure in Check the heater.

the Heating system. (heating without Reboot the automaton.
temperature rising during 2 minutes).
System communication with I/O board is out Check HGB and FSC/ALL boards connection.
at automaton power on.
Reboot the automaton.

Auto clean cycle initiated. Press on and wait the end of the cycle.
Note: Auto Clean frequency is adjustable in

SETTINGS/OTHERS screen.
Appears when trying to run a cycle after an

Press on and perform a control cycle.
emergency stop.
Control cycle is mandatory after emergency stop.
Trying to run a cycle while another one is in

Press on and wait the end of the cycle in
progress.
progress before to run another cycle.
Cycle stopped upon user request (emergency stop

Press on and perform a control cycle.
button).
Press on to cancel the message.

Vacuum failure during bath draining
1. RBC/PLT bath is not drained:

 Perform VACUUM TEST then verify
the value (410 mb +/-5%) and the
stability of the vacuum.
 Check tub #2 and #14 (clogged,
pinched or disconnected).
 Check valve #2 (clogged or
damaged).
2. WBC bath is not drain:

 Perform VACUUM TEST then verify
 Check tub #1 and #14 (clogged,
pinched or disconnected).
 Check valve #1 (clogged or
damaged)

3. RBC/PLT and WBC baths are not

drained:
 Perform a VACUUM TEST; verify the
value (410 mb +/-5%) and the
 Check tub #14 (clogged or pinched).
 If the vacuum is close to “0”, check
Valve #8 functionality, check
tub#5;18;19;24:29 (bad connection
or disconnected).
Vacuum stability check failed during syringe

vacuum
Perform VACUUM TEST then verify the value (410
mb +/-5%) and the stability of the vacuum.
Vacuum failure during the needle rinsing.

1. Perform VACUUM TEST then verify

2. Check tub #10 (pinched or clogged).
3. Check if diluent comes properly to
rinsing head.
4. Check if needle O-ring damaged.
5. Check if needle guide released.
6. Check valve #3 (clogged or
damaged).
Vacuum failure at the beginning of bath draining

1. Verify diluent comes to the baths.

3. Verify valve #1; #2 (do not close
properly).

Vacuum failure during counting phase in analysis

Press on to cancel the message
cycle.
Pressure failure during waste syringe draining.

Press on to cancel the message

3. Check valve #7 (clogged or
damaged).
System detects waste container full.

Press on , empty the waste container and reset
waste level in reagent menu.
Note: Waste container capacity is adjusted in

reagent menu.
The system detected the fluidic door opened. If the fluidic door is closed, verify the good
functionality of the fluidic door switch and its
physical adjustment.
The adjustment of the HGB LED is failed. 1. Verify that WBC bath is filled with
diluent during HGB LED adjustment.
2. Change HGB board.
3. Change WBC bath.
Note: HGB LED light flux must be included in the
range [18000 ; 22000] after gain adjustment.
The system detected HGB command error just after

Press on to cancel the window.
switching ON the instrument.
1. Check the HGB cable connection on

Note: When this error occurs the HGB LED is not
HGB board.
lighted ON. 2. Check the HGB cable connection on
CPU board.
3. Replace the HGB board.
4. Replace the CPU board.

Motors initialization is not done. Press on to cancel the window.
Perform motors initialization.

Ex: Instrument was switched ON with fluidic
door opened.
Steps lost on the needle motor. Press on to cancel the window.
1. Check if mechanical hard point on

needle axis.
2. Check if needle bent.
3. Check the needle belt tension.
4. Check O-ring in rinsing head.
Needle cannot join its home Press on to cancel the window.
1. Bad connection on needle sensor

wiring.
2. Needle sensor is damaged.
3. Bad connection on needle motor
wiring.
4. Needle motor is damaged.
5. Mechanical hard point on needle axis
which prevents the needle to reach
the home pos.
Needle not in home position detected before Press on to cancel the window.
to perform cycle.
Perform a motor initialization.
Home needle detection error Press on to cancel the window.
Perform motor init.
If the issue occurs again, change the sensor.

Cycle prime all is mandatory after clean out. Press on and perform a prime all cycle.
Rinse cycle is mandatory after drain cycle. Press on and perform a rinse cycle.
Steps loss on the sampling X axis. Press on to cancel the window.
1. Check if mechanical hard point on X

axis.
2. Perform greasing of the worm wheel.
3. Check for damaged part cramping
sampling X axis to move freely.
Home sampling X axis detection error Press on to cancel the window.
Perform motor initialization.
Start-up cycle mandatory after shutdown. Press on and perform Start-up cycle.
Steps loss on the Syringe motor Press on to cancel the window.

1. Perform greasing of the worm wheel.

2. Perform pistons greasing.
3. Check if tub pinched on Syringe.
4. Check if valve do not open or clogged
on syringe.
syringe module.
6. Verify if syringe motor damaged.
Syringe can’t join its home Press on to cancel the window.
1. Bad connection on syringe sensor

wiring.
2. Syringe sensor is damaged.
3. Bad connection on syringe motor
wiring.
4. Syringe motor is damaged.
5. Mechanical hard point on syringe axis
which prevents the needle to reach
the home pos.
Home syringe detection error Press on to cancel the window.
Perform motor init.
System detects there’s no bleach during Press on and perform motor init.
bleach cycle.
Redo bleach cycle adding bleach when system
require.
Steps loss on the Waste motor Press on to cancel the window.
1. Perform greasing of the wormwheel.

2. Perform pistons greasing.
3. Check if tub pinched on Waste
Syringe.

4. Check if valve do not open or clogged

on waste syringe.
waste syringe module.
Verify if waste syringe motor damaged.
Waste cannot join its home Press on to cancel the window.
1. Bad connection on waste syringe

sensor wiring.
2. Waste syringe sensor is damaged.
3. Bad connection on waste syringe
motor wiring.
4. Waste syringe motor is damaged.
5. Mechanical hard point on waste
syringe axis cramping waste syringe
to reach the home pos.
Home waste syringe detection error Press on to cancel the window.
Perform motor init.
System detected a command error of valve 1. 1. Verify all connections concerning

valve 1 (valve and CPU connectors).
2. Verify electrical continuity on the two
cables of the valve 1.
3. Replace valve 1.

6. Replace valve 2.


3. Replace valve 3.

3. Replace valve 4.

3. Replace valve 5
1. Verify all connections concerning

System detected a command error of valve 6. 2. Verify electrical continuity on the two
Replace valve 6.

3. Replace valve 7.

4. Replace valve 8.

4. Replace valve 9.

System detected a command error of valve 1. Verify all connections concerning

10. valve 10 (valve and CPU connectors).
3. Replace valve 10.
System detected a command error of valve 1. Verify all connections concerning

11. valve 11 (valve and CPU connectors).
3. Replace valve 11.
1. Redo a second startup.

System detected a background value out of 2. Perform bleach cycle.
range on one or few measured parameters. 3. Troubleshoot on the high background
of the concerned parameter.
System detected start up cycle was not done. Run START UP cycle.
System detected cleaner reagent bottle out of Replace cleaner reagent.

date after opening.
System detected lyse bottle is empty. Change lyse reagent bottle.
System detected lot number already used Redo using another lot number

System detected cleaner bottle is empty Change cleaner bottle.
System detected lyse reagent bottle out of Change lyse bottle.

date after opening.
System detected default during reagent data Correct the default entering good data
recording
System detected diluent reagent is almost You still can perform ten analysis then system will
empty. generate diluent is empty
System detected failure during reagent data Correct the default entering good data
recording
System detected diluent bottle is empty. Change diluent bottle.

System detected default during reagent data Correct the default entering good data
recording
System detected diluent reagent bottle out of Change diluent bottle.

date after opening.
System detected Cleaner bottle almost empty You still can perform ten analysis then system will
generate cleaner is empty
System detected lyse bottle almost empty You still can perform ten analysis then system will
generate lyse is empty

Chapter 10 SHUTDOWN
1. From the main menu, press SHUTDOWN .
2. The following progress bar will be displayed.
3. The hydraulic circuit of the counting and the optic modules will be rinsed.
4. Counting chambers will be filled with cleaner reagent.
5. At the end of the shutdown cycle, MISPACOUNTPLUS will automatically turn off.
6. Shut Down can be set to be automatically performed (see ).
NOTE: After a shutdown, it is not possible to perform an analytical cycle without performing startup first
MISPACOUNTPLUS must stay at least with cleaning solution for three hours every 24
hours.

Introduction:
The present document, dedicated to the user and service engineers, details the needed information in
matter of error message.
Error message occurs when system detects a default which could have an impact on the instrument
(damage) or on the quality of the analysis (false result). When the system detects a default, any action in
progress is then stopped immediately and the user is alerted by a popup displayed on screen.
On cycle completion (auto stop or emergency stop), all motors, aperture currents, optical LED and valves are
deactivated. All error messages are saved in the error log file and can be consulted, printed or save on USB
flash drive as needed.
ERROR Message have the following requirements:
Prompt Trigger Consequence Troubleshooting
MESSAGE Condition to Consequence of

What can be done to fix the issue
DISPLAYED raise the error the error
 Error popup always uses confirmation type

 Prompt tittle is ERROR
if for any reason the user wants to stop the instrument, an emergency stop can be generated by simple
pressing on the on/off button.
Cycle stopped
upon user 1. Fix the Issue
CYCLE STOPPED CYCLE IS
request
BY USER STOPPED 2. Press on
(emergency 3. Select FLUIDICS CONTROL CYCLE
stop button).
If the adjustment fails, a prompt informs the operator, the background check is done ignoring the
haemoglobin. The haemoglobin is invalidated until an adjustment of the LED succeeds.

1. Press on
the operator 2. Check presence of diluent inside WBC
STARTUP bath.
STARTUP cycle can run Sample.
FAILED – HGB 3. Check all connections
is failed HGB is
ADJ FAILED 4. Perform HGB adjustment
invalidated 5. Check HGB LED (change is necessary)
6. Check HGB board (change is necessary)
7. Check CPU board (change is necessary)
If the level is higher, the instrument will display the following prompt:
STARTUP 4. Press on
FAILED – STARTUP cycle the operator 5. Perform a second startup
BACKGROUND is failed can run Sample 6. Perform bleach cycle
CHECK FAILED 7. Troubleshoot on the concerned
parameter
When a cycle is trigged while another one is being executed, the following prompt is displayed
Trying to run
CYCLE IS 1. Press on
CYCLE BUSY cycle while
REFUSED 2. Wait the end of the cycle in progress then
cycle is running retry
When trying to run a cycle after an emergency stop the following prompt is displayed

Appears when
trying to run a
cycle after an
FLUIDICS emergency
CONTROL stop. Fluidic CYCLE IS
1. Press on
CYCLE NOT control cycle is REFUSED
2. perform FLUIDICS CONTROL cycle
DONE mandatory
after
emergency
stop.
If the user attempts to run a cycle after a clean out without performing a Prime ALL, the following prompt is
displayed (except when the user requests STARTUP)
PRIME ALL Cycle prime all ANALYSIS CYCLE

CYCLE NOT is mandatory IS NOT 1. Press on
DONE after clean out ALLOWED 2. Select PRIME ALL cycle
If the Startup is not done and the user attempts to run analysis cycle, the following prompt is displayed
ANALYSIS CYCLE
STARTUP NOT STARTUP cycle
IS NOT 1. Press on
DONE is not done
ALLOWED 2. Select STARTUP cycle
If the user attempts to run a cycle after a drain or a drain flow cell without performing a rinse cycle, the
following prompt is displayed
Rinse cycle is
mandatory ANALYSIS
RINSE CYCLE
after drain CYCLE IS NOT 1. Press on
NOT DONE
cycle or drain ALLOWED 2. Select RINSE cycle
flow cell.
Note: Short fluidic mechanical initialization when automaton is switched on.


If the fluidic door is open, the short fluidic mechanical initialization is not done and the following prompt is
is displayed
Motors
MOTOR INIT Emergency
initialization is 1. Press on
NOT DONE stop.
not done. 2. Perform a motor initialization
Note: Opening the fluidic door generates an emergency stop of the running cycle, except during the waiting
message of a specific cycle (for example bleach cycle and clean out).
Fluidic door
opened
FLUIDIC during a
EMERGENCY
DOOR fluidic cycle 1. Press on
STOP
OPENED except when 2. Close FLUIDIC DOOR
the user is
asked for
Note: Opening door moves probe up if the probe is down (Probe will be up at any door opening for all user’s
login).
When the fluidic door is required to be closed, the following prompt is displayed once
Fluidic door is
PLEASE CLOSE open and/or is
FLUIDIC DOOR required to be N/A 1. Press on
TO CONTINUE closed to run 2. Close FLUIDIC DOOR
the cycle
When fluidic door is still not closed after “PLEASE CLOSE FLUIDIC DOOR TO CONTINUE” occurred once

CYCLE AUTO Fluidic door is

N/A 1. Press on
STOPPED still not closed
2. Close FLUIDIC DOOR
Note: When the measured pressure is out of the range defined in the fluidic cycle, an error is raised and the
current cycle is stopped.
Vacuum failure during the needle rinsing
1. Press on
Vacuum 2. Check the presence of diluent in rinse head fluidic
circuit
NEEDLE failure
Emergency 3. Check if tub clogged or pinched in rinse head
RINSING during the
stop. fluidic circuit
DEFAULT needle 4. Check the rinse pump
rinsing 5. Check the inline pressure sensor
6. Check valves #3,4,5,6,18,12 in check sensors
screen
Vacuum failure during bath draining in analysis cycle
1. Press on
Vacuum 2. Check the presence of reagent in counting bath
failure 3. Check the CPU pressure sensor
DRAIN
during bath Emergency 4. Check if tub clogged or pinched in drain bath
BATH
draining in stop. fluidic circuit
DEFAULT 5. Check valve #1 or 2 depending from the bath
analysis
cycle concerned
6. Check valve #17 and 8 (atmosphere circuit)
7. Check for leak on waste syringes
Vacuum failure during counting phase in analysis cycle

Vacuum
failure
VACUUM 1. Press on
during Emergency
COUNTING 2. Check the CPU pressure sensor
counting stop.
DEFAULT 3. Check for leak on waste syringes
phase in 4. Check syringe module
analysis cycle
Vacuum failure at begin of bath draining
Vacuum
failure at Emergency 1. Press on
NO DILUENT 2. Replace the empty diluent container by a new one
begin of bath stop.
draining
Vacuum failure during test syringe cycle
Vacuum 1. Press on
SYRINGE failure 2. Check for leak on waste syringes
Emergency 3. Check for disconnected tubing
VACUUM during test
stop. 4. Check the CPU pressure sensor
DEFAULT syringe
5. Check syringe module
cycle.
Pressure failure during test syringe cycle (on draining waste from syringe)

Pressure 1. Press on
failure 2. Check valve #7
WASTE during test 3. Check for pinched tubing on waste line
Emergency
DRAINING syringe cycle 4. Check syringe module
stop.
DEFAULT (on draining 5. Check the CPU pressure sensor (Change CPU as
waste from necessary)
syringe)
Pressure failure during pump test
1. Press on
Pressure
PUMP Emergency 2. Check for pinched or clogged tubing on pump line
failure during
DEFAULT stop. 3. Check valve #3 (change as necessary)
pump test 4. Check in line sensor (change as necessary)
5. Change the pump (change as necessary)
Detected air on bleach cycle
Detected air
NO BLEACH Emergency 1. Press on
on bleach
IN THE BATH stop. 2. Redo bleach cycle
cycle. 3. Check for leakage
Note: When the relative difference of the measured pressure to the previous check is out of the range
defined in the fluidic cycle, an error is raised and the current cycle is stopped.
Vacuum stability check failed during syringe vacuum

Vacuum
stability 1. Press on
SYRINGE
check 2. Check for leak on waste syringes
VACUUM Emergency
failed 3. Check for disconnected tubing
STABILITY stop.
during 4. Check the CPU pressure sensor
DEFAULT 5. Check syringe module using CHECK SYRINGE
syringe
vacuum option
Vacuum stability check failed during counting vacuum
Vacuum
stability
VACUUM 1. Press on
check
COUNTING Emergency 2. Check the CPU pressure sensor
failed
STABILITY stop. 3. Check for leak on waste syringes
during 4. Check syringe module using CHECK SYRINGE
DEFAULT
counting option
vacuum
Vacuum stability failed during the needle rinsing
7. Press on
Vacuum 8. Check the presence of diluent in rinse head fluidic
stability circuit
NEEDLE 9. Check for tubing clogged/pinched in rinse head
failed Emergency
RINSING fluidic circuit
during the stop.
DEFAULT 10. Check the rinse pump USING TEST PUMP option
needle 11. Check the inline pressure sensor USING TEST
rinsing. PUMP option
12. Check valves #3,4,5,6,18,12 in check sensors
screen
Note: When a motor is supposed to return to its home position, if it is not detected, or step loss is detected,
an error is raised, and the current cycle is stopped.
Note: When a motor is in its home position while it is not supposed to be, an error is raised, and the current
cycle is stopped.

Motors initialization is not done.
Motors 1. Press on
MOTOR INIT Emergency
initialization is 2. Perform SYSTEM INIT
NOT DONE stop.
not done.
Steps lost on the needle motor.
1. Press on
Steps lost on
Emergency 2. Check for mechanical hard point on needle
NEEDLE GAP the needle
stop. movement
motor. 3. Check Needle using CHECK NEEDLE option
4. Check Needle sensor CHECK NEEDLE option
Needle cannot join its home
Needle cannot 1. Press on

NEEDLE
join its home Emergency 2. Check Needle using CHECK NEEDLE option
HOME NOT
stop. 3. Check Needle motor using CHECK NEEDLE
FOUND option
4. Check CPU (change as necessary)
Needle not in-home position detected before to perform cycle.
1. Press on
Needle not in- 2. Check Needle sensor using CHECK NEEDLE
home position option
NEEDLE HOME Emergency
detected 3. Check needle sensor using CHECK NEEDLE
NOT FOUND stop.
before to option
perform cycle. 4. Check CPU ²
Home sensor had been detected but the sensor is not in the home position

Home needle 1. Press on

NEEDLE HOME Emergency
detection 2. Check needle sensor using CHECK NEEDLE
ERROR stop.
error option
3. Check CPU
Steps loss on the rocker motor.
1. Press on
Steps loss on
Emergency 2. Check Rocker module using CHECK ROCKER
ROCKER GAP the rocker
stop. option
motor. 3. Check Rocker sensor using CHECK ROCKER
option
Home sensor had been detected but the sensor is not in the home position
1. Press on
Home rocker 2. Check Rocker sensor using CHECK ROCKER
ROCKER HOME Emergency
detection option
ERROR stop.
error 3. Check CPU (change as necessary)
Message when Rocker cannot join its home
1. Press on
Home rocker 2. Check Rocker sensor using CHECK
ROCKER HOME
cannot join its Emergency stop. ROCKER option
NOT FOUND
home 3. Check Rocker module using CHECK
ROCKER option
Message when Home sensor had been detected but the sensor is not in the home position

1. Press on
SYRINGE HOME Home Syringe
Emergency stop. 2. Check syringe sensor using CHECK
ERROR detection error
SYRINGE option
Message when Syringe Home not found error
1. Press on
Home Syringe 2. Check Syringe sensor using CHECK
SYRINGE HOME
cannot join its Emergency stop. SYRINGE option
NOT FOUND
home 3. Check Syringe module using CHECK
SYRINGE option
Message when Steps loss on Syringe motor
1. Press on
Steps loss on
2. Check Syringe module using CHECK
SYRINGE GAP the syringe Emergency stop.
SYRINGE option
motor. 3. Check Syringe sensor using CHECK
SYRINGE option
Note: Communication with the HGB, FSC and ALL boards is checked (unplugged board).
Message when a communication error occurs

1. Press on
2. Reboot the automaton
The system 3. Check HGB and FSC/ALL boards
BOARDS detected connection.
EMERGENCY
COMMUNICATION communication 4. Check HGB and FSC/ALL cable
STOP
ERROR error with If one of the cable above was
satellite board(s). disconnected, please connect the
cable to the board and reboot the
instrument
5. Check CPU board.
Message when error is detected during the valve command, the fluidic cycle is stopped and an error
message informs the operator.
1. Press on
Valve [EV label] is 2. Verify connections on valve [EV
LABEL]
EV [EV LABEL] not connected
EMERGENCY 3. Verify electrical continuity on [EV
COMMAND (plugged or out
STOP LABEL]
ERROR of order) or is 4. Replace valve [EV LABEL]
short-circuited 5. Check fuse (change as necessary)
6. Check CPU board (change as
necessary)
Note Heating Temperature Security and control Reagent temperature are checked against overflow.
Message when Reagent temperature > 60°C


A prompt
informs the
MAXIMUM user: Reagent 1. Press on
REAGENT temperature 2. Check cable connections
Reagent 3. Check the heater (change as necessary)
TEMPERATURE over 60.
temperature > Note: Instrument must be restarted after issue
REACHED. Heating is
60°C fixing to unlock the error
HEATER IS stopped.
STOPPED.
Run sample
inaccessible.
Note Heating system is checked regarding efficiency (heating without temperature rising).
Message when Reagent heating at 100% and no temperature increase during 2mn
Heating is
Reagent 1. Press on
stopped; A
heating at 2. Check cable connections
prompt informs 3. Check the heater
REAGENTS 100% and no
the user: 4. Check CPU
HEATER IS temperature
Reagent Note: Instrument must be restarted after issue
STOPPED increase during
heating failed. fixing to unlock the error
2mn (less than
Run sample
0.5 increase)
inaccessible.

Message when the reagent temperature is < to target – 2.5°C
Run sample
inaccessible
(except with
SERVICE or
higher rights).
REAGENT These
TEMPERATURE conditions
If the reagent
IS OUT OF disable the 1. Press on
temperature
RANGE. probe down 2. Wait for set temperature reached
is < to target –
function.
ANALYSIS ARE 2.5°C
NOT ALLOWED The error
prompt
appears when
user goes in
screen where
you can run
sample.
Message when the instrument temperature is < to 18°C or > to 36.5°C
If the
INSTRUMENT 1. Press on
instrument A prompt
TEMPERATURE 2. Wait for set temperature reached
temperature informs the
OUT OF 3. Check cable connections
is < to 18°C or user 4. Check the heater
RANGE.
> to 36.5°C 5. Check CPU
Note:This prompt is not displayed when logged as manufacturing or R&D.
Message when Lyse bottle is empty

PLEASE No cycle can be 1. Press on

CHANGE LYSE: If Lyse is launched 2. Change the lyse bottle
CONTAINER IS empty (except in R&D 3. Perform lyse priming
EMPTY Mode)
Message when Cleaner bottle is empty
PLEASE
CHANGE No cycle can 1. Press on
CLEANER: If Cleaner is be launched 2. Change the cleaner bottle
empty (except in R&D 3. Perform cleaner priming
CONTAINER IS Mode)
EMPTY
Message when Diluent container is empty
PLEASE
No cycle can 1. Press on
CHANGE
If Diluent is be launched 2. Change the diluent container
DILUENT:
empty (except in R&D 3. Perform diluent priming
CONTAINER IS
Mode)
EMPTY
Message when Diluent Expiration date is attempted

PLEASE
REPLACE 1. Press on
Diluent No cycle can
DILUENT Expiration be launched 2. Change the diluent container
CONTAINER: date is (except in R&D 3. Perform diluent priming
attempted Mode)
REAGENT IS
OUT OF DATE
Message when Cleaner Expiration date is attempted
PLEASE
REPLACE no cycle can be 1. Press on
Cleaner
CLEANER launched 2. Change the lyse bottle
Expiration date
CONTAINER: (except in R&D 3. Perform lyse priming
is attempted
REAGENT IS Mode)
OUT OF DATE
Message when Lyse Expiration date is attempted
PLEASE
REPLACE no cycle can be
Lyse Expiration 1. Press on
LYSE launched 2. Change the lyse bottle
date is
CONTAINER: (except in R&D 3. Perform lyse priming
attempted
REAGENT IS Mode)
OUT OF DATE
Message when Lyse Open date is exceeded

Trigger Consequence Prompt Troubleshooting
PLEASE
no cycle can be REPLACE
Lyse Open 1. Press on
launched LYSE 2. Change the lyse bottle
date is
(except in R&D CONTAINER: 3. Perform lyse priming
exceeded
Mode) REAGENT IS
OUT OF DATE
Message when Diluent Open date is exceeded
PLEASE
REPLACE no cycle can
Diluent Open 1. Press on
DILUENT be launched 2. Change the diluent container
date is
CONTAINER: (except in R&D 3. Perform diluent priming
exceeded
REAGENT IS Mode)
OUT OF DATE
Message when Cleaner Open date is exceeded
PLEASE
REPLACE no cycle can 1. Press on
Cleaner Open
CLEANER be launched 2. Change the cleaner bottle
date is
CONTAINER: (except in R&D 3. Perform cleaner priming
exceeded
REAGENT IS Mode)
OUT OF DATE
Message when Incorrect code entry

REAGENT 1. Press on
Incorrect
AUTENTIFICATION NA 2. Enter the correct code then validate
code entry
FAILED
Note: This is not applied when customer is not configured.
Message when Reagent lot (lot number + bottle SERIAL) already exists in the reagent log
Reagent lot
(lot number + 1. Press on
REAGENT bottle SERIAL) 2. Change the diluent data
NA
ALREADY USED already exists 3. Perform diluent priming
in the reagent
log
Message when Invalid Lot Number for lyse
LOT NUMBER Invalid Lot 1. Press on

IS NOT FOR Number for NA 2. Enter the correct number then validate
LYSE lyse
Message when Invalid Lot Number for diluent


DILUENT diluent
Message when Invalid Lot Number for cleaner

CLEANER cleaner
Message when the user attempt to register more than 12 users
MAXIMUM The user

NUMBER OF attempt to 1. Press on
NA 2. Suppress one or few users
USERS IS register more
REACHED than 12 users
Message when an error occurred during software upgrade
An error 1. Press on
SOFTWARE occurred 2. Try again
UPGRADE during N/A 3. Check USB key (change as necessary)
ERROR software 4. Contact Customer Service Department
upgrade
When logged in Biolo, Operator or Service level, it is not possible to able/disable an actioner from the “check
sensors” screen while a cycle is in progress.

The user
attempts to ACTION ON
manually THE ACTIONER 1. Press on
CYCLE BUSY
activate/de- IS NOT 2. Wait the end of the cycle in progress then
active an PERFORMED retry
actioner
Note: Adjustment process fails if the number of cells counted during the first or second counting phase are
less than
 400 pulses per counting period for WBC

 300 pulses per counting period for RBC
Message when the RBC adjustment fails
1. Press on
2. Try again
RBC RESISTIVE 3. Perform a bleach cycle
Resistive gain
GAIN Default gain is 4. Check the latex solution (polluted)
adjustment
ADJUSTMENT applied 5. Check counting head
failed
FAILED 6. Check cable and connections
Message when the WBC adjustment fails

1. Press on
WBC 2. Try again
RESISTIVE Resistive gain 3. Perform a bleach cycle
Default gain is 4. Check the latex solution (polluted)
GAIN adjustment
applied 5. Check counting head
ADJUSTMENT failed
6. Check cable and connections
FAILED
Note: The HGB adjustment fails when the LED flow cannot be adjusted within target +/- tolerance.
The haemoglobin is invalidated until an adjustment of the LED successes.
Message when the HGB LED adjustment fails
The The HGB

invalidated 1. Press on
HBG LED adjustment of 2. Proceed to HGB LED adjustment again
the HGB LED until an
ADJUSTMENT 3. Verify the presence of diluent in WBC bath
failed. adjustment of
FAILED 4. Check cable and connections
the LED 5. Check HGB LED
successes. 6. Check HGB board
Message when the OPT LED adjustment fails
The DIF
Measurement
The 1. Press on
OPT LED is invalidated
adjustment of 2. Proceed to OPT LED adjustment again
ADJUSTEMENT until an
the OPT LED is 3. Verify the presence of diluent in WBC bath
FAILED adjustment of 4. Check cable and connections
failed.
the LED 5. Check optical bench
successes.

Message when during counting phase more than one sequence has a reference out of the fixed range
(Target ±10%), the following prompt is displayed
If more than
one sequence 1. Press on
GAIN has a 2. Try again
ADJUSTMENT reference out N/A 3. Check cable and connections
FAILED of the fixed 4. Check optical bench
range (Target
±10%)
Message when during counting phase, pulse count is less than 200
1. Press on
GAIN if pulse count 2. Check for tubing pinched or clogged
ADJUSTMENT in the area is N/A 3. Check for cables connections
FAILED not sufficient 4. Check for optic nozzle clogged
5. Change latex solution
6. Check for CPU (change as necessary)

MISPACOUNTPLUS ANALYSE CYCLE - FLUIDIC SEQUENCES DESCRIPTION STEP BY STEP
1. SAMPLE ASPIRATION AND SAMPLING PROBE CLEANING
STEP ACTION COMMENT

HGB Green led located in WBC counting chamber is switched
1 HGB led on
on
2 Read HGB Blank HGB led measure through diluent for HGB reference
3 Syringe DOWN Vacuum tank draining
4
Syringe DOWN Sample aspiration in the sampling probe
Sampling probe UP + Syringe
5 Sampling probe outside rinsing with syringe and pump
UP + Pump ON
Sampling module moves to
6 Sampling module and sampling probe in home position
home position
Sequence duration: 5 secs, cycle duration: 5 secs
2. SAMPLING PROBE MOVES TO WBC COUNTING CHAMBER AND WASTE CHAMBERS DRAINING
STEP ACTION COMMENT

1 Syringe UP Waste chambers draining
2 Sampling probe located above the WBC counting chamber
WBC counting chamber
Sampling probe moves to rinsing position in WBC counting
3 Sampling probe DOWN
chamber
4 Syringe UP Sampling probe outside rinsing with syringe in WBC counting
chamber
Sequence duration: 2 secs, cycle duration: 7secs

3. WBC COUNTING CHAMBER DRAINING AND SAMPLING PROBE POSITIONING
STEP ACTION COMMENT
1 Syringe DOWN + Pump ON WBC counting chamber draining + Transfer tubing draining
Sampling probe moves to dilution position in WBC counting

bath
Sequence duration: 3.25 secs, cycle duration: 10.25 secs
4. FIRST DILUTION AND BUBBLING IN WBC COUNTING CHAMBER
STEP ACTION COMMENT

Diluent (by entry on WBC counting chamber) in WBC
1 Syringe UP
counting chamber for first dilution
Sample + Diluent (through the sampling probe) dispense in
2 Syringe UP
WBC counting chamber for dilution
3 Syringe UP First sample and diluent bubbling in WBC counting chamber
5. FIRST DILUTION ASPIRATION, SAMPLING PROBE CLEANING
STEP ACTION COMMENT

RBC counting chamber partial draining and diluent
1 Syringe DOWN
removal on WBC counting chamber entry
Syringe DOWN and Sampling
2 First dilution aspiration in sampling probe
probe UP
Sampling probe UP + Syringe Sampling probe moves to home position and sampling
3
UP + Pump ON probe outside rinsing
4 Sampling module in RBC dilution position
RBC counting chamber
5 Sampling probe DOWN Sampling probe moves to rinsing position in RBC counting
chamber

6. LYSE IN WBC COUNTING CHAMBER AND BUBBLING
STEP ACTION COMMENT

Lyse dispense in WBC counting chamber + Sampling probe
2 Syringe UP
outside rinsing in RBC counting chamber
3 Syringe UP End of lyse dispense in WBC counting chamber
4 Syringe UP Second bubbling in WBC counting chamber
7. RBC COUNTING CHAMBER DRAINING
STEP ACTION COMMENT

RBC counting chamber draining and lyse removal on WBC
1 Syringe DOWN + Pump ON
counting chamber
Sampling probe moves to dilution position in RBC counting
chamber
8. SECOND DILUTION AND BUBBLING IN RBC COUNTING CHAMBER
STEP ACTION COMMENT

Diluent (by entry on RBC counting chamber) in RBC counting
1 Syringe UP
chamber for RBC dilution
First dilution sample + Diluent (through the sampling probe)
2 Syringe UP
dispense in RBC counting chamber for dilution
3 Measurement current ON
4 Optical LED ON
Syringe UP + Sampling probe Third bubbling in WBC counting chamber + Sampling probe in
5
UP home position
6 Syringe UP Bubbling in RBC counting chamber + counting heads rinsing
Sequence duration: 7 secs, cycle duration: 32.5 secs
9. FIRST COUNTING

STEP ACTION COMMENT

Waste chambers at atmosphere Pressure control to “0” in waste chambers before vacuum
1
pressure creation for counting
WBC dilution transferred to the optical bench and diluent
2 Syringe DOWN
removal on RBC counting chamber
First and second dilutions (present in counting chambers)
3 Syringe DOWN
aspiration through WBC and RBC counting heads
4 Wait 500ms Resistive counting vacuum stabilization
5 Check pressure Pressure control before resistive counting
Cells counting acquisition during aspiration through WBC and
6 Start first RES counting
RBC counting heads
7 Stop first RES counting
Sequence duration: 8 secs, cycle duration: 40.5 secs
10. BACK FLUSH IN COUNTING HEADS AND SECOND COUNTING
STEP ACTION COMMENT

Back flushes with diluent in counting heads to make sure
1 Syringe UP
potential clogs did not happen during first counting sequence
First and second dilutions (present in baths) aspiration
2 Syringe DOWN
through WBC and RBC counting heads
Syringe UP and start optical Injection of transferred sample in optical bench, WBC
3
WBC differentiation differentiation
Cells counting acquisition during aspiration through WBC and
4 Start second RES counting
RBC counting heads
5 Stop second RES counting
11. COUNTING HEADS CLEANING
STEP ACTION COMMENT

1 Counting Heads cleaning Diluent behind counting heads is replaced by cleaner
Stop Optical WBC
2
differentiation
3 Measurement current OFF
4 Optical LED OFF

12. WBC COUNTING CHAMBER DRAINING AND FILLING
STE
ACTION COMMENT
P
WBC counting chamber and transfer tubing draining,
Syringe DOWN + Pump ON +
1 sampling probe moves to rinsing position in RBC counting
Sampling probe DOWN
chamber
2 Syringe UP WBC counting chamber filling with diluent
Back flushes with cleaner for cleaning counting heads after
3 Syringe UP
counting sequences
Sequence duration: 2.5 secs, cycle duration: 55 secs
13. RBC COUNTING CHAMBER DRAINING AND FILLING
STEP ACTION COMMENT

1 Syringe DOWN + Pump ON RBC counting chamber draining and optical transfer priming
Diluent (through Sampling probe outside) dispense in RBC
2 Syringe UP
counting chamber + waste chambers draining
3 Sampling probe UP Sampling probe moves to home position
4 Pump ON Sampling probe drying through rinsing head
5 Syringe UP Diluent dispense in rinsing head to rinse the Sampling probe
14. SAMPLING MODULE MOVES TO WBC COUNTING CHAMBER AND SAMPLING PROBE INITIALIZATION FOR
NEXT SAMPLE
STEP ACTION COMMENT

1 Sampling module in sampling position
home position
2 Syringe UP Syringe moves to home position
3 Sampling probe DOWN Sampling probe in sampling position, ready for next sampling
Waste chambers to atmosphere
4
pressure
5 End of analytic cycle
Sequence duration: 2.5 secs, cycle duration: 60 secs

Dismantling of different units

1. Vacuum tank.
MISPACOUNTPLUS is equipped with an additional vacuum tank allowing to increase the quantity of vacuum
additionally to the one generated by the waste syringe. This additional reserve of vacuum is necessary to
answer to the instrument needs in matter of vacuum due to the implementation of the optical module.

2. Pump
MISPACOUNTPLUS is equipped with an additional pump increasing the efficiency of the needle rinsing.
This pump is a 24Volts membrane pump designed to support both air and liquids
The pump is installed on the rinse head fluidic circuit, on aspiration side. It is used to aspirate the diluent
pushed on the rinse head to perform to needle washing. Diluent used to clean the needle will be evacuated
to the waste container.
The vacuum generated by the pump is checked and controlled regularly during the analysis cycle.
To control the pump vacuum, the MISPACOUNTPLUS is equipped with an in-line pressure sensor connected
on the same line, checking the vacuum generated by the pump.
Rinse Pump
In line Pressure Sensor


It is possible to check verify or control the good functionality of the MISPACOUNTPLUS pump.
Going in check sensors screen, select TEST PUMP to switch on the pump, then check the value in INLINE
PRESSURE field.
The value displayed in INLINE PRESSURE field must be -300±50mb


3. Heating System
To perform the five-differential optical reading, diluent and lyse reagents must to be heated.
The reason why is to increase and accelerate the action of the lyse and for that reason, MISPACOUNTPLUS
is equipped with a additional heating module.
Heating system is internally equipped with two separated fluidic circuits respectively dedicated for diluent
and lyse.
Internal diluent fluidic circuit is much longer than internal lyse fluidic circuit due to the difference of
volumes which must be heated. The Difference of volumes which must be heated come from the
difference of volumes necessary to perform the analysis.
Reagent Heating System is equipped with the following elements

It is possible to check verify or control the good functionality of the MISPACOUNTPLUS Heating system.
Going in check sensors screen, REAGENT TEMP field is dedicated to the display of the heater temperature.
HEAT field is dedicated to the display of the heating power in percentage (0 for the min, 100 for the max).
DILUENT TEMP field is dedicated to the display of the diluent temperature injected in WBC counting
chamber.
Optic Module Disassembly

The following instructions will guide you to dismantle and assemble the Optic Module.
Optic Module replacement.

Optic Module dismantling.
Tools/Material  Torx tool size T20
DISMANTLING INSTRUCTION
Action Step Reference
Prerequisite 1. Perform a drain flow cell cycle. 1. Refer to the instruction.

2. Switch off the instrument and
disconnect the power supply. 2. Refer to the instruction.
3. Remove the fluidic door. 3. Refer to the instruction.
4. Remove the reagent door. 4. Refer to the instruction.
5. Remove the top cover. 5. Refer to the instruction.
6. Remove the reagent plate. 6. Refer to the instruction.
Dismantling 1. Disconnect the four connectors

from detection boards.
Dismantling

2. Disconnect the optic LED

connector.
Dismantling 3. Disconnect the diluent tubings

(#35B & #35C) from the Y
connector.
Dismantling 4. Disconnect the dilution tubings

(#34A) from the flow cell.

Dismantling 5. Locate plastic clip which maintain

the tubing #33E in good position.
NOTE: Position of tubing 33E is very

important, it allows keeping potential air
bubbles in the higher part of the tubing
thus avoiding pushing it up into the flow
cell.
Dismantling 6. Remove tubing 33E from plastic

clip.
Dismantling 7. Disconnect tubing #33E from the

Y connector.

Dismantling 8. Disconnect tubing #36 from the

check valve.
Dismantling 9. Remove the ground cable fixation

screw.
Dismantling 10. Release (do not remove) the

optic module fixations screws.

Dismantling 11. Remove the optic module from

the unit.
Completion NOTE: To avoid any risk of clotting, it is

recommended to dry the flow cell using
an air aerosol.
7. Connect the air aerosol to the

tubing #35B and dry it.

tubing #35C and dry it.

tubing #33E and dry it.

Optic Module assembling

 Adjustable Torque screw
tool
ASSEMBLING INSTRUCTION
Prerequisite 1. Follow the instruction of Optic 1. Refer to the instruction.

Module dismantling.
Assembling 1. Install the optic module on the

unit.
Assembling 2. Secure the module with the

fixation screws tighten to
dedicated torque.
20 cNm
Assembling 3. Install the ground cable fixation

screw tighten to dedicated torque.
150 cNm
Assembling 12. Connect tubing #36 on the check #36

valve.

Assembling 13. Connect tubing #33E on the Y

connector.
#33E
Assembling 14. Install tubing 33E on plastic

clip.
#33E
Assembling 15. Notice the position of tubing

#33E and repeat.
NOTE: Position of tubing 33E is very

important. Allows keeping potential air
bubbles in the higher part of the tubing
thus avoiding pushing it up into the
flow cell.
Assembling 16. Connect tubings #34A on the

flow cell.
#34A

Assembling 17. Connect tubings #35B & #35C

on the Y connector.
#35c
#35B
Assembling 18. Connect the optic LED

connector.
Assembling 19. Connect the four connectors on

detection boards.
NOTE: Yellow coaxial must be connected

on ALL board. Black coaxial must be
connected on FSC board.
Completion 1. Install the reagent plate 1. Refer to the instruction.

2. Install the top cover 2. Refer to the instruction.
3. Install the reagent door 3. Refer to the instruction.
4. Install the fluidic door 4. Refer to the instruction.
5. Refer to the instruction.

5. Connect the power supply and

switch on the instrument 6. Refer to the instruction.
6. Perform FLUIDIC CONTROL 7. Refer to the instruction.
cycle. 8. Refer to the instruction.
7. Perform a startup cycle.
8. Follow the instruction of Optic
Module Adjustment.
Optic Module Adjustment.

 Torx tool size T10
 Adjustable Torque screw tool
 Transverse adjustment tool
 Latex beads Ø7µm
 G-Cal
Prerequisite 1. Follow the instruction of 2. Refer to the instruction.

Optic Module assembling.
Adjustment 1. Select:
SERVICE/TECHNICIAN/ADJUST WBC
then select ADJ LED.
Adjustment 1. Select the green tick to validate the

optic LED adjustment
Adjustment 2. After LED adjustment, new OPTIC

LED and ALL value are implemented

Adjustment 3. When the optic LED adjustment is

done, place a tube of latex 7µm in
sampling position then press on
CHECK OB.
Adjustment 4. After CHECK OB cycle completion,

the latex mark is displayed on
screen.
NOTE: the latex mark must have the

same shape than the one on the picture
of the right. If it is the case go directly
to the point 17 from this instruction.
NOTE: If the latex mark has this shape it

means the transverse adjustment must
be done.
Adjustment 1. If the latex mark does not have a

correct shape, remove the flow
cell protection cover fixation
screw.

Adjustment 2. Release (do not remove) the transverse

support fixation screw ½ turn
Adjustment 5. Press on ADJ.OB
Adjustment 6. The following window is displayed, select

the green tick to validate.
Adjustment 7. The following window is displayed

Adjustment 8. Place the stopcock in close position.
Adjustment 9. Place a tube of latex 7µm in sampling

position then select the green tick to
validate

Adjustment 10. When the curves appear on screen, you can begin
the adjustment turning the transverse adjustment
tool on both side.
Adjustment 11. The goal is to adjust the transverse position to

have the 2 curves as flat as possible and as close
as possible from each other
Adjustment 12. Redo the adjustment as necessary until to obtain

the good result.

Adjustment 13. Once the transverse adjustment done, place the

stopcock in vertical position (open).
Adjustment 14. Place a tube of latex 7µm in sampling position

then press on CHECK OB.
15. After CHECK OB cycle completion, the latex mark

is displayed on screen.
NOTE: the latex mark must have this shape to

consider the optic module as conform. If it is the case
go directly to Xx from this instruction.

Adjustment 16. When the latex mark is correct, screw the

transverse support fixation screw to the dedicated latex mark conform
torque.
15 cNm
Adjustment 3. install the flow cell protection cover

fixation screw tighten to the dedicated
torque.
40 cNm

Adjustment 17. Place a vial of G-Cal in sampling position then

press on ADJ.WBC.
Adjustment 18. At the end of the cycle FSC and ALL gain values
are updated
Adjustment 19. Run with fresh blood and verify the good position
of the scattergram.

4. Optical module
Allowing to give 5 parts differentials of WBC, MISPACOUNTPLUS instrument is equipped with an Optical
bench.
Optical bench is composed with 3 parts, illumination, detection and flow cell.
Detection part Illumination part

Flowcell

VERY IMPORTANT NOTE
Only the complete optical module (equipped with the optical flow cell) can be replaced on
MISPACOUNTPLUS.
It means that it is impossible to replace independently the illumination, detection and or flowcell
sub-assembly which compose the optical bench and it is obvious that it is impossible to replace
independently the different parts which compose these sub- assemblies.
These sub-assemblies are adjusted during manufacturing process with a specific adjustment
tooling which allows to adjust and control each part independently and to adjust and control the
complete module assembly.
Illumination part
 Uniform intensity distribution at the interrogation zone.
 Transverse Light beam on Sample Flow adjustment.
 Blue wavelength available at low cost.
 Large LED chip emitting area è no critical positioning tolerances.
LED
Detection part

 Light collection.
 Beam spill ting.
 FSC & ALL measurement
Photo diode
Flowcell
- Metallic nozzle for sample injection.
- Nozzle aperture diameter is 80µm.
- Sample flow with perfect cylindrical shape


Counting Module Disassembly
The following instructions will guide you to dismantle and assemble all elements of the counting module.
RBC Electrode.
Tools/Material  Torx tool T10
Removal
Action Steps Reference
Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN BATHS

2. Switch off the instrument and disconnect the 2. Refer to the instruction
main power cord.
3. Open the fluidic door. 3. Refer to the instruction
Electrode 1. Turn the electrode plastic holder from a quarter

RBC turn anticlockwise.
2. Pull on it and remove the RBC electrode
assembly from the RBC counting chamber.
3. Remove the ground cable fixation screw (1) of

the RBC electrode.
1
4. Disconnect the RBC coaxial connector (2) from

the counting manifold and then remove the RBC
electrode from the instrument. 2
5. Replace the RBC electrode respecting the

reverse instruction.

RBC Counting Chamber replacement
Removal

2. Switch off the instrument and disconnect the
main power cord.
3. Open the fluidic door.
4. Remove the RBC electrode.
RBC 1. Release the maintaining screw (1) of the

Chamber chambers protection and remove it.
2. Remove the three remaining fixation screws (2)

of the RBC chamber. 2 2
3. Pull on the RBC chamber and remove it.
4. Replace the RBC chamber following the reverse 2

instruction.
Note: Verify the presence of the O-ring on the red

fitting before to perform the reassembling.

Note: Verify the presence of the three O-rings on

the chamber before to perform the reassembling.
38
38 38
38

Important note: Screw back the four

fixation screws respecting a torque
to 50cNm
5. Reinstall the chambers protection.
50cNm

RBC Counting Head replacement

 4K05 Microparticles
Removal

main power cord.
4. Remove the RBC electrode. 4. Refer to the instruction
5. Remove the RBC counting chamber. 5. Refer to the instruction
RBC 4. Remove the two fixations screws (1) of the RBC

Counting counting head.
Head
NOTE: RBC counting head is identified with a red

dot.

5. Remove the RBC counting head using pliers to

pull it if necessary.
6. Replace the RBC counting head following the

Note: Verify the presence of the O-ring before to

perform to the reassembling.

Important note: Screw back the two

fixation screws, respecting a torque
to 30cNm.
30 cNm
7. From the same screen, using Latex particles

5µm, perform RBC gain adjustment.
NOTE: Compare the size of the latex particles

with the value in the field DIAMETER and
modify as necessary.

WBC Electrode replacement
Removal

main power cord.
Electrode 1. Turn the electrode plastic holder from a quarter

WBC turn anticlockwise.
2. Pull on it and remove the WBC electrode
assembly from the WBC counting chamber.
3. Remove the ground cable fixation screw (1) of

the WBC electrode. 1
4. Disconnect the WBC coaxial connector (2) from

the counting manifold and then remove the RBC
electrode from the instrument. 2
5. Replace the WBC electrode respecting the


WBC Counting Chamber replacement
Removal
Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN BATHS 1. Refer to the instruction.

2. Switch off the instrument and disconnect the 2. Refer to the instruction.
main power cord. 3. Refer to the instruction.
1. Open the fluidic door. 4. Refer to the instruction.
2. Remove the WBC electrode.
3. Remove the chambers protection.
4. Do HGB GAIN Adjustment
WBC 1. Disconnect the HGB cable connector (1).

Chamber
2. Remove the three remaining screws (2) of the

WBC chamber. 2 2
3. Pull on the WBC chamber and remove it.
4. Replace the WBC chamber following the reverse

instruction.

Note: Verify the presence of the O-ring on the red

fitting before to perform the reassembling.
38
Note: Verify the presence of the three O-rings on

the chamber before to perform the reassembling.
4 4

Important note: Screw back the four

fixation screws respecting a torque
to 50cNm
50cNm

WBC Counting Head replacement

 4K07 Microparticles
Removal
Prerequisite 1. SERVICE/TROUBLESHOOTING/DRAIN BATHS 1. Refer to the instruction.

main power cord. 3. Refer to the instruction.
4. Remove the WBC electrode.
5. Remove the WBC counting chamber.
6. perform WBC GAIN Adjustment
6. Refer to the instruction
WBC 1. Remove the two fixations screws (1) of the WBC

Counting counting head.
Head
2. Remove the WBC counting head using pliers to

pull it if necessary.

3. Replace the WBC counting head following the

Note: Verify the presence of the O-ring before to

perform to the reassembling.
38
Important note: Screw back the two 2

fixation screws, respecting a torque
to 30 cNm.

30 cNm
4. From the same screen, using Latex particles

7µm, perform WBC gain adjustment
pressing on ADJUST.
NOTE: Compare the size of the latex particles

with the value in the field DIAMETER and
modify as necessary.

Counting Holder
Removal

2. Switch off the instrument and disconnect the
main power cord.
3. Open the fluidic door.
4. Remove the complete equipped RBC chamber.
5. Remove the complete equipped WBC counting
chamber.
Counting 1. Remove the two nuts (1) which maintain the

Holder RBC and WBC coaxial connectors on the
counting holder. 1
2. Once the two nuts removed, push on the coaxial

connectors to remove them from the counting
holder.
3. Remove the ground terminal fixation screw (2).

4. Disconnect all tubings from the counting holder.
5. Remove the three fixation screws (3) of the

counting holder then, remove it.
3 3
6. Equip the new counting manifold with all
elements and reassemble it following the

Heater Module Disassembly
The following instruction will guide you to dismantle and assemble the heater module.
Heater replacement.
Heater dismantling.
Prerequisite 10. Drain the instrument from any 7. Remove pick up tubing’s from containers and
liquids. perform prime all.
15. Remove the reagent plate
Dismantling 20. Disconnect the tubing’s from the

heater.
Dismantling 21. Disconnect the heater connector.

Dismantling 22. Remove the three (3) fixation

screws located on fluidic side,
then remove the heating system
from the unit.

Heater assembling

Heater dismantling.
Assembling 23. Install the three (3) fixation

screws located on fluidic
side.
100 cNm
Assembling 24. Connect the heater

connector.

Assembling 25. Connect the tubings on the heater.

4A
22
11
7
Completion 1. Install the reagent plate. 1. Refer to the instruction.

2. Install the top cover. 2. Refer to the instruction.
3. Install the reagent door. 3. Refer to the instruction.
4. Install the fluidic door. 4. Refer to the instruction.
5. Connect the power supply and switch on the 5. Refer to the instruction.
instrument.
6. Select REAGENT/PRIME ALL.
7. Verify the good working in check sensors.

In Line Pressure Sensor Disassembly
The following instructions will guide you to dismantle and assemble all elements of the In Line Pressure
Sensor.
In Line Pressure Sensor replacement.

In Line Pressure Sensor dismantling.
Tools/Material  NA
Prerequisite 16. Switch off the instrument and disconnect the 13. Refer to the instruction.
power supply.
20. Remove the reagent plate 17. Refer to the instruction.
Dismantling 26. Disconnect the two tubing #10B and #10C

from the sensor. 10B
10C
Dismantling 27. Disconnect the electrical connector.

Dismantling 28. Remove the pressure sensor from the unit.
In Line Pressure Sensor assembling
Prerequisite 3. Follow the instruction of In Line Pressure 4. Refer to the instruction.

Sensor
Assembling 1. Connect the electrical connector.

Assembling 2. Connect the tubings #10B and #10C to the

sensor. 10B
10C

instrument.
6. Verify the good working in check sensors
screen
7. Select SERVICE/TECHNICIAN/ADJUST
OTHERS, Enter the altitude in dedicated field,
then press ADJUST.

Pump Disassembly
The following instructions will guide you to dismantle and assemble the rinse pump.
Pump replacement.
Rinse pump dismantling.
power supply.
25. Remove the front cover. 22. Refer to the instruction.
27. Remove the second reagent plate.
Dismantling 29. Remove the two fixation screws

Dismantling 30. Disconnect the two tubings #10D & #23E

from the pump.
#23E #10D
Dismantling 31. Disconnect the electrical connector.

Dismantling 32. Remove the pump from the unit.

Rinse pump assembling
Prerequisite 4. Follow the instruction of the Rinse pump 5. Refer to the instruction.
dismantling
Assembling 33. Connect the electrical connector.
Assembling 34. Connect the two tubings #10C & #10B) from
the pump.
#10C #10B
#10B

NOTE: Pay attention to tubing connections on

rinse pump.
#10C
35. Install the pump with the two fixation screws.
NOTE: No specific torque for these screws, just

screw until the mechanical contact with the
rubber washers, then perform 1/4 turn more.
Completion 1. Install the second reagent plate. 1. Refer to the instruction.

2. Install the reagent plate. 2. Refer to the instruction.
instrument
7. Verify the good working in check sensors
screen.

Stopcock Disassembly
The following instructions will guide you to dismantle and assemble the stopcock element.
Stopcock replacement.
Stopcock dismantling.

 Cutting pliers
Prerequisite 28. Switch off the instrument and 24. Refer to the instruction.
disconnect the power supply.
Dismantling 36. Locate the stopcock at the back

on the top of the instrument.
Dismantling 37. Using cutting pliers, remove the

two fasteners which maintain
the stopcock.

Dismantling 38. Unscrew the Luer connector of

the tubing 33B.
#33B
Dismantling 39. Unscrew the Luer connector of

the tubing 3C.
#3C
Dismantling 40. Remove the stopcock from the

unit.

Stopcock assembling
Prerequisite 5. Follow the instruction of Stopcock 6. Refer to the instruction.

dismantling.
.
Assembling 1. Install the Luer connector of the

tubing 3C.
#3C
Assembling 2. Unscrew the Luer connector of the

tubing 33B.
#33B

Assembling 41. Install two fasteners to maintain

the stopcock.
Completion 1. Install the reagent plate 1. Refer to the instruction.

3. Install the reagent door 3. Refer to the instruction.
4. install the fluidic door 4. Refer to the instruction.
5. Connect the power supply and 5. Refer to the instruction.
switch on the instrument
6. Perform a fluidic control cycle. 6. Refer to the instruction.

Ambient Temperature Sensor Disassembly
The following instructions will guide you to dismantle and assemble the ambient and reagent temperature
sensors.
Ambient Temperature Sensor Replacement.

Ambient temperature sensor dismantling.
power supply.
39. Remove the second reagent plate.
Dismantling 42. Locate the instrument temperature sensor

screwed on the base pate.
Dismantling 43. Remove the fixation screw.
44. Remove the temperature sensor from the

unit.

NOTE: Ambient temperature sensor is directly

connected to the CPU board

Ambient temperature sensor assembling
Prerequisite 6. Follow the instruction of Ambient 7. Refer to the instruction.

temperature sensor dismantling.
Assembling 4. Install the ambient temperature sensor with

the fixation screw tighten to the dedicated
torque.
NOTE: Ambient temperature sensor is directly

connected to the cpu board.
150 cNm

11. Install the front cover. 10. Refer to the instruction.
14. Connect the power supply and switch on the
instrument.
15. Verify the good working of the sensor in
check screen.
16. Verify the good working of the ambient
temperature sensor in check sensors.

Diluent temperature sensor replacement.
Diluent temperature sensor dismantling.
power supply.
7. Remove the second reagent plate. 7. Refer to the instruction.
Dismantling 1. Locate the diluent temperature sensor close

to the back of WBC chamber.
Dismantling 2. Remove the fixation screw.

Dismantling 3. Disconnect the temperature sensor.
Dismantling 4. Remove the temperature sensor from the

unit.

Diluent temperature sensor assembling
Prerequisite 1. Follow the instruction of Diluent 1. Refer to the instruction.

temperature sensor dismantling.
Assembling 1. Install the diluent temperature sensor

with the fixation screw tighten to the
100 cNm
dedicated torque.
Assembling 5. Connect the diluent temperature

sensor.

4. Install the front cover. 4. Refer to the instruction.
7. Connect the power supply and switch 7. Refer to the instruction
on the instrument.
8. Verify the good working in check
sensor screen.

Discharge Tubing Disassembly
The following instructions will guide you to dismantle and assemble the Discharge Tubing.
Discharge Tubing replacement.

Discharge Tubing dismantling.
Tools/Material  Cutting pliers
Dismantling 45. Locate the discharge tubing at the

back of the unit, fixed with 2
fasteners on the vacuum tank
support.
Dismantling 46. Remove the 2 fasteners.

Dismantling 47. Disconnect the discharge tubing from the

stopcock.
Dismantling 48. Disconnect the discharge tubing from the Y

connector, then remove it from the unit.

Discharge Tubing assembling
Prerequisite 7. Follow the instruction of Discharge Tubing 8. Refer to the instruction.

dismantling.
Assembling 49. Install the discharge tubing on the vacuum

tank support with the 2 fasteners.
Assembling 50. Connect the discharge tubing on the Y

connector.

Assembling 51. Connect the discharge tubing on the

stopcock.

instrument.
13. Select REAGENT/PRIME LYSE. 11. Refer to the instruction
14. Perform a startup cycle. 12. Refer to the instruction

CPU board Disassembly
The following instructions will guide you to dismantle and assemble the different electronic boards.
CPU board replacement.

CPU board dismantling.
Tools/Material  Torx tool size T10.
Prerequisite 45. Select SERVICE/BACKUP & 1.

RESTORE/BACKUP and follow the
instructions displayed.
NOTE: USB flash drive is mandatory to

backup.
46. Switch off the instrument and 3. Refer to the instruction.

47. Follow the instruction of Front
Cover Dismantling.
Dismantling 52. Remove the 7 CPU board fixation

screws.

Dismantling 53. Remove the 6 remaining connectors

from CPU board.
54. Remove the CPU board from
location

CPU board assembling
Prerequisite 8. Follow the instruction of CPU 9. Refer to the instruction.

board dismantling.
9. Note the serial number of the
new CPU board.
Assembling 6. Install the CPU on location.

7. Connect the 6 cables to the CPU.
Assembling 8. Install the CPU board fixation

screws.
Completion 1. Follow the instruction of Front 1. Refer to the instruction.

Cover Assembling.
2. Connect the main cord and switch 2. Refer to the instruction.
on the instrument.
3. Select SERVICE/TECHNICIAN/
SYSTEM CONFIG, then enter the

new CPU serial number and

instrument serial number. 3.
4. Select
SERVICE/BACKUP&RESTORE/RESTO
RE and follow the instructions 4.
displayed.
NOTE: USE the same USB flash drive

than the one used to backup.
5. Select 5.
SERVICE/TECHNICIAN/ADJUST
OTHERS, enter the altitude (where
the instrument is located) in the
dedicated field and select ADJUST.
6.
6. From the same screen, using Latex
particles 7µm, perform WBC gain
adjustment pressing on ADJUST.
NOTE: Compare the size of the latex

particles with the value in the field
DIAMETER and modify as necessary.
7.
7. From the same screen, using Latex
particles 5µm, perform RBC gain
adjustment.
NOTE: Compare the size of the latex

particles with the value in the field
DIAMETER and modify as necessary.

8. Select 8.
OTHERS, in the field HGB select
ADJUST.
9.
9. Validate the adjustments by

selection of the green tick.
10.
10. Select
SERVICE/TECHNICIAN/ADJUST/ADJ.L
ED to perform optic LED
adjustment. 11.
11. Place a vial of G-Cal in sampling

position then press on ADJ.WBC.

12.
12. At the end of the cycle FSC and

ALL gain values are updated
13. Run with fresh blood and verify

the good position of the
scattergram

USB board replacement.

USB board dismantling.
power supply.
2. Follow the instruction of Front Cover 2. Refer to the instruction.
Dismantling.
Dismantling 1. Disconnect the USB cable coming from CPU.

2. Remove the USB ground cable fixation screw.
Dismantling 3. Remove the 2 USB board fixation screws.

Dismantling 4. Remove the USB board from the instrument.
USB board assembling
Prerequisite 1. Follow the instruction of USB board 10. Refer to the instruction.
dismantling.
Assembling 1. Connect the USB cable coming from CPU.
Assembling 2. Install the 2 USB board fixation screws.

3. Install the USB ground cable fixation screw.

Completion 1. Follow the instruction of Front Cover 7. Refer to the instruction.

Assembling.
2. Using an USB flash drive, verify the good

working of the new USB board

Rear Panel board replacement.

Rear Panel board dismantling.
power supply.
2. Follow the instruction of reagent plate 2. Refer to the instruction.
dismantling.
Dismantling 1. Remove the 3 fixation screws of the Rear

Panel Board.

Dismantling 2. Disconnect the cables from the board.
Dismantling 3. Remove the board from the unit.

Rear Panel board assembling
Prerequisite 1. Follow the instruction 1. Refer to the instruction.

of Rear Panel board
dismantling
Assembling 1. Connect the cables

onto the board.

Assembling 2. Install the board with

the fixation screws.
Completion 1. Follow the 1. Refer to the instruction.

instruction of
Reagent Plate 2. Refer to the instruction.
Assembling.
2. Connect the main cord
and switch on the
instrument.
3. Verify the good
working of the
associated board
function.

HGB Board replacement.

HGB Board dismantling.

Dismantling 4. Disconnect the 2 cables from the

HGB board.
Dismantling 5. Remove the HGB board fixation

screw, then remove the board
from the unit.

HGB Board assembling
Prerequisite 1. Follow the instruction of HGB 1. Refer to the instruction.

Board dismantling
Assembling 1. Install the board with the

fixation screw.
15cNm
Assembling 2. Connect the 2 cables connectors.
Completion 1. Connect the main cord and 1. Refer to the instruction.

switch on the instrument.
2. Close the fluidic board. 2. Refer to the instruction.
3. Select 3.
OTHERS, in the field HGB select
ADJUST.

Sampling Module Disassembly
The following instructions will guide you to dismantle and assemble all elements of the sampling module.
1 Sampling probe replacement.

1.1 Probe dismantling.
Tools/Material  Beak pliers
Sampling 55. Pull on the top of the sampling

Probe probe and release it from the
Dismantling sampling probe carriage.

Sampling 56. Pull on the rinse head to release it

Probe from the rocker module.
Dismantling
Sampling 57. Pull the sampling probe up until to

Probe remove it from the rinse head.
Dismantling

Sampling 58. Disconnect the tubing 9 from the

Probe sampling probe.
Dismantling
NOTE: Because tubing 9 material

is polyurethane, do not try to pull on it
to disconnect from the sampling
probe.
Use a beak pliers or an equivalent tool
as described below.
Sampling 59. Insert the beak pliers or an

Probe equivalent tool in the space
Dismantling located between the base of the
probe and the end of tubing 9.
NOTE: Move the tool to the down,

leveraging on tubing 9 moving it
through the top.
Sampling 60. Once tubing 9 begins moving onto

Probe the probe, shift the tool
Dismantling transversely and do the action
than on point 5.
61. Repeat the same operation until

the complete disconnection of
tubing 9.

NOTE: To prevent any risk of leakage,

it is recommended to replace the O-
ring located in the rinse head when
replacing the sampling probe.
1.2 Probe assembling
Tools/Material  Torx T06

 Cutting pliers

sampling probe dismantling.
11. Follow the instruction of O-ring 12. Refer to the instruction.
replacement.
Sampling 1. Using cutting pliers, refresh

Probe tubing 9 from 3 millimeters. Tubing 9
Assembling
NOTE: To facilitate connection of

tubing 9, insert Torx tool size T06 to
enlarge internal Ø.
Torx T06

Sampling 12. Connect tubing 9 to the probe

Probe leaving a space from one
Assembling millimeter between end of
tubing and the probe base.
1mm
Needle base
Sampling 13. Insert the probe in the rinse

Probe head.
Assembling
Sampling 14. Install the rinse head on the

Probe rocker.
Assembling

Sampling 15. Engage the base of the probe on

Probe the probe carriage, respecting
Assembling the good positioning of the
probe.
Needle
Needle base carriage
NOTE: Notice the specific shape of

the probe base and the carriage.
Sampling 16. Press on the probe base to

Probe assure the good positioning on
Assembling the carriage.
Completion 1. Install the front cover. 1. Refer to the instruction.

5. Connect the power supply cord 5. Refer to the instruction.
and switch on the instrument.
6. Perform a startup cycle. 6. Refer to the instruction.

2 Probe’s O-ring replacement

2.1 Probe’s O-ring dismantling

Probe’s 1. Pull on the top of the sampling

O-ring probe to release it from the
Probe’s 2. Pull on the rinse head to release it

O-ring from the rocker.
Dismantling

Syringe Module Disassembly
The following instructions will guide you to dismantle and assemble all elements of the syringe module.
Syringe body & Pistons replacement.
Syringe body & pistons dismantling.
Prerequisite 53. Drain the instrument from any 45. Remove pick up tubings from their
liquids. container and select REAGENT/PRIME ALL.
57. Remove the reagent plate.
58. Remove the top cover.
Syringe 62. Remove the four fixation screws of

Body the syringe body.
&
Pistons
Dismantling

Syringe 63. Remove the assembly body/pistons.

Body
&
Pistons
Dismantling
Syringe 64. Remove the pistons from the syringe

Body body.
&
Pistons
Dismantling

Syringe body & pistons assembling
Prerequisite 17. Follow the instruction of Syringe 13. Refer to the instruction.
body & pistons dismantling.
.
Syringe Body 9. Install the pistons in the syringe

& body.
Pistons
Assembling
Sampling Piston
Diluent Piston
Waste Piston
Waste Piston
Lyse Piston
Syringe Body 10. Install the assembly on the syringe
& motor holder.
Pistons
Assembling Note: Be sure all the 16 O-rings are in
well in good position before installing
the syringe body.

3. Install the body on the syringe

motor holder.
NOTE: Pay attention to the good

positioning of the 5 pistons in the
carriage before to secure the module
with fixation screws.
4. Secure the body with the six

fixations screws.
NOTE: Tighten first the six screws to

35 cNm respecting the order.

35cNm
NOTE: Tighten the screws from the

top to 60 cNm.
60cNm
Completion 1. Install the fluidic door. 1. Refer to the instruction.

4. Connect the power supply and 4. Refer to the instruction.
5. Select
SERVICE/TROUBLESHOOTING/SYRI
NGE GREASING and perform a
pistons greasing.
6. Select
SERVICE/TROUBLESHOOTING/CHE
CK SENSORS/TEST VACUUM and
control the stability of the vacuum
in waste pressure field.
7. Select REAGENT/PRIME ALL.

Syringe motor holder replacement.
Syringe motor holder dismantling.
Prerequisite 1. Drain the instrument from any liquids. 1. Refer to the instruction.
6. Follow the instruction of Syringe body 6. Refer to the instruction.
& pistons dismantling
Syringe 1. Remove the ground cable fixation

motor screw.
holder
Dismantling
Syringe 2. Disconnect the sensor cable

Motor connector.
Holder
Dismantling

Syringe 3. Disconnect the motor cable

Motor connector.
Holder
Dismantling
Syringe 4. Disconnect all tubings from Syringe

Motor motor holder
Holder
Dismantling
Syringe 5. Move the piston carriage downward.

Motor
Holder
Dismantling

Syringe 5. Release (do not remove) the four

Motor fixation screws
Holder
Dismantling
Syringe 6. Remove the syringe motor holder

Motor from the unit.
Holder
Dismantling

Syringe motor holder assembling
Prerequisite 1. Follow the instruction of Syringe 1. Refer to the instruction.

motor holder dismantling.
.
Syringe 1. Place the syringe motor holder in

Motor position.
Holder
Assembling
Syringe 2. Secure the module with the four

Motor fixation screws, tighten to the
Holder dedicated torque.
Assembling
20 cNm
Syringe 3. Connect all tubings on syringe

Motor motor holder
Holder
Assembling

Syringe 6. Connect the motor cable

Motor connector.
Holder
Assembling
Syringe 7. Connect the sensor cable

Motor connector.
Holder
Assembling

Syringe 8. Install the ground cable fixation

Motor screw tighten to the dedicated
Holder torque. 150 cNm
Assembling
Completion 1. Connect the power supply and 1. Refer to the instruction

2. Follow the instruction of Syringe 2. Refer to the instruction
body & pistons assembling.

Slotted optical sensor replacement.

Slotted optical sensor dismantling.

Slotted 1. Disconnect the syringe sensor

optical connector.
sensor
Dismantling
Slotted 2. Remove the syringe sensor fixation

optical screw.
sensor
Dismantling

Counting Valves Module Disassembly
The following instructions will guide you to dismantle and assemble counting valves module.
Counting valves replacement.
Removal
liquids. containers and select REAGENT/ PRIME
ALL.
disconnect the main power cord. 3. Refer to the instruction.
Counting NOTE: Counting valve module is

Valves composed with 2 valves 2/2-1.6mm-
Disassembling M2.5 .
1. Disconnect the valve connector.
NOTE: As an example, the following

instruction will describe the way how to
replace the counting valve N°1, proceed
as the same in case of change of valve
N°2.
1
2. Remove the two fixation screws (1) of
the valve solenoid.
3. Remove the solenoid from the valve

body.

2
4. Remove the two fixation screws (2) of 2
the valve body.
5. Remove the valve body from the

manifold.
6. Install the new valve following the

NOTE: Be careful to the mounting

direction of the valve body.
The “NC” inscription must be oriented
through the bottom for the two valves.
1. Tighten the two fixation screws of the

valve body, respecting a torque to 40cNm
40cNm.
2. Install the valve solenoid on the valve

body.
NOTE: Be careful to the mounting

direction of the valve solenoid.
Connector must be oriented through the
right.

3. Tighten the two fixation screws of the 25cNm

valve solenoid, respecting a torque to
25cNm.
Counting Valves Module Replacement
Removal
liquids. containers and select REAGENT/ PRIME ALL.

disconnect the main power cord. 4. Refer to the instruction.
5. Remove the top cover.
Counting 1. Disconnect the 4 tubings from

Valves valves #1 and #2.
Module
2. Disconnect the two valves

connectors.

3. Remove the two fixation screws (1)

of the counting valves module and
remove it.
1
4. Reassemble the counting valves
module following the reverse
instruction.
NOTE: Torque for fixation screws of 1

counting valve module is 100 cNm.
Slotted 4. Remove the syringe sensor from the

optical unit.
sensor
Dismantling

Slotted optical sensor assembling

Slotted optical sensor
dismantling.
Slotted 1. Install the syringe sensor on

optical the motor holder.
sensor
Assembling
Slotted 2. Secure the syringe sensor

optical with the fixation screw
sensor tighten to the dedicated
Assembling torque.
100 cNm

Syringe 5. Connect the syringe sensor

optical connector.
sensor
Assembling
Completion 1. Install the reagent plate 1. Refer to the instruction

2. Install the top cover. 2. Refer to the instruction
3. Install the fluidic door. 3. Refer to the instruction
4. Install the reagent door. 4. Refer to the instruction
5. Connect the power supply 5. Refer to the instruction
6. Select
SERVICE/TROUBLESHOOTING
/CHECK SYRINGE to control
the good working of the new
sensor

Probe’s 3. Unscrew totally the probe guide and

O-ring release it from the rinse head.
Dismantling
Probe’s 4. Pull the sampling probe up, removing at

O-ring the same time the probe, the probe guide
Needle
Dismantling and the probe’s O-ring from the rinse
head.
Guide
O-ring
Probe’s NOTE: Be careful to the gasket located in the

O-ring rinse head. It must stay in position during
Dismantling probe guide and O-ring removal but check it
out its presence.
Gasket
5. Remove the Probe’s O-ring from the

probe.

2.2 Probe’s O-ring assembling

Sampling probe’s O-ring
dismantling.
1. Install the probe’s O-ring on the

probe without removing the
probe guide.
Probe’s 2. Once O-ring is installed on the

O-ring probe, the assy. is ready to be Needle
Assembling installed. Guide
3. Install the assy. On rinse head.

4. Screw the probe guide on the

rinse head.
NOTE: It is not necessary to

overtighten the probe guide on the
rinse head, gently screw it until the
mechanical stop.
Probe’s 5. Put back the rinse head in the

O-ring rocker.
Assembling
Probe’s

O-ring 6. Insert the base of the probe on

Assembling the probe carriage, respecting
the good positioning of the
probe.
Needle
carriage
Needle base
NOTE: Notice the specific
shape of the probe base and the
carriage.
Probe’s 6. Press on the probe base to

O-ring assure it is well pressed in the
Assembling carriage.

6. Select SERVICE/SYSTEM INIT

3 Rocker replacement
3.1 Rocker dismantling.
 Torx T10
 Cutting pliers

Sampling 1. Pull on the top of the sampling

Probe probe and release it from the
Sampling 2. Pull on the rinse head to release it

Probe from the rocker module.
Dismantling

Sampling 3. Pull the sampling probe up until to

Probe remove it from the rinse head.
Dismantling
Rocker 4. To avoid any damage during

Dismantling operations, place the probe as
shown on picture.

Rocker 1. Disconnect tubing 10 from the rinse

Dismantling head.
Rocker 2. Disconnect tubing 21 from the rinse

Dismantling head, then remove the rinse head.
Rocker 3. Remove tubing 10 & 21 from

Dismantling rocker’s location.
Rocker 4. Remove the two (2) fixation screws

Dismantling of the rocker guide.
2 1

Rocker 5. Move the rocker Backward.

Dismantling
Rocker 6. Rotate the rocker’s guide clockwise

Dismantling then pull on it to remove it.
Rocker 7. Move the sampling probe carriage

Dismantling down on the middle of the rocker.

Rocker 8. Release (do not remove) the fixation

Half a Turn
Dismantling screw of the lower pulley.
Rocker 9. Remove the three (3) fixation

Dismantling screws of the rocker protection,
then remove it.
2
3 1
Rocker 10. Manually move the rocker

Dismantling forward.

Rocker 11. Release (do not remove) the

Dismantling fixation screw of the belt
tensioning system.
One Turn
Rocker 12. Remove the lower fixation

Dismantling screw of the metallic plate
which maintains the rocker on
the ball bearing.
Rocker 13. Release (do not remove) the

Dismantling second fixation screw of the
metallic plate which maintains One Turn
the rocker on the ball bearing

Rocker 14. Push manually on the metallic

Dismantling plate to rotate it.
Rocker 15. Keeping the metallic plate

Dismantling rotated, pull on the rocker to
disengage it from the ball
bearing.
Rocker 16. Using cutting pliers, remove the

Dismantling plastic fastener which
maintains the sampling probe
sensor cable on the board.

Rocker 18. Disconnect the sampling probe

Dismantling sensor cable.
Rocker 17. Remove rocker module from

Dismantling the unit.
3.2 Rocker assembling.
 Adjustable Torque tool

 Torx T20
 Cutting pliers
Prerequisite 1. Follow the instruction of rocker 1. Refer to the instruction.

dismantling.

Rocker 1. Grease the rocker gear.

Assembling
Rocker 2. Install the rocker.

Assembling
NOTE: maintain the metallic plate

rotated to insert the rocker onto the
ball bearing
Rocker 3. Once the ball bearing is engaged

Assembling in its location, gently push on the
rocker until the metallic plate
can rotate freely through the
down.

Rocker 4. Secure the plate, with the

Assembling second fixation screw and tight TORQUE = 80 cNm
both screw to the dedicated
torque
2
Rocker 5. Move the rocker forward and

Assembling backward to engage the two (2)
gears in each other.
NOTE: Do not force on the rocker to

engage the two (2) gears. It could
damage the teeth.
Find the good positioning allowing
the gearing.

Rocker 6. Install the rocker guide in

Assembling position by tilting it.
Rocker 7. Once in position, rotate the

Assembling guide to the horizontal position.
Rocker 8. Secure the guide with the two

Assembling (2) fixation screws.
NOTE: Do not tight at this

stage.
2
1
Rocker 9. Move the rocker over the guide.

Assembling

Rocker 10. Push up the guide against the

Assembling rocker.
NOTE: Pay attention to the guide

positioning.
It mustn’t be too high, it could
generate excessive friction with the
rocker.
Set a gap of one (1) millimeter
between the guide and the rocker.
Front view
1mm
1mm
Rocker 11. Secure the two (2) screws to the TORQUE = 80 cNm
Assembling dedicated torque.
2
1
NOTE: After tightening the fixation

screws of the guide, move the
rocker back and forth and verify
there is no mechanical hard point.
3 1

Rocker 18. Manually move the rocker

Assembling forward.
Rocker 19. Using adjustable torque tool,

Assembling secure the fixation screw of the
belt tensioning system to the
dedicated torque.
3 cNm
3 cNm

Rocker 20. Using adjustable torque tool,

Assembling secure the fixation screw of the 20 cNm
lower pulley to the dedicated
torque.
Rocker 21. Once lower pulley fixation screw

Assembling secured to 20 cNm, apply the
same torque of 20 cNm to the
fixation screw of the belt
tensioning system.
20 cNm
20 cNm

Rocker 12. Pass tubing 21 in the first

Assembling fastener.
Rocker 13. Pass tubing 21 in the second

Assembling fastener, then in the dedicated
place of the rocker.
NOTE: Hemostat can be

used to facilitate the operation.
2
3
4
Rocker 14. Refresh tubing 21 from 3

Assembling millimeters, then connect it to
the rinse head.

Rocker 15. Pass tubing 10 in the first

Assembling fastener.
Rocker 16. Pass tubing 10 in the second

Assembling fastener, then in the dedicated
2
place of the rocker.
NOTE: Hemostat can be used to

facilitate the operation.
3
4
Rocker 17. Pass tubing 10 in the dedicated

Assembling hole of the rocker.

NOTE: Refresh tubing 21 from 3

millimeters before to connect. 55 mm
NOTE: Respect a length of 55mm

between the end of tubing 10 and
the hole on the rocker.
Rocker 18. Connect tubing 10 to the rinse

Assembling head.
NOTE Once connected, tubing 10

must make a loop as shown on the
right picture.
NOTE: if the length of 55mm is not

respected (too short) the tubing 10
can be pinched at the rinse head.
NOTE: If the length of 55mm is not

respected (too long) the loop of
tubing 10 is too large.
In this configuration, tubing 10 can

be pinched against the front plate
during rocker movements.

NOTE: When the length of 55mm is

respected rocker can move freely
without risk of tubing 10 pinched
with the front plate.


4 Probe belt & probe carriage replacement

4.1 Probe belt & probe carriage dismantling
 Torx T08
Tools/Material
 Torx T10
Removal
Prerequisite 1 Follow the instruction of Rocker dismantling. 1. Refer to the instruction.
Probe belt 2. Remove the screw which maintains the

& metallic shaft in location.
Carriage
Dismantling
Probe belt 3. Remove the metallic shaft from the rocker.

&
Carriage
Dismantling

Probe belt 4. Remove the fixation screw of the lower

& pulley.
Carriage
Dismantling
NOTE: Be careful to the scare nut located at the

back of the rocker while removing the lower
pulley fixation screw.
Probe belt 5. Release the lower pulley from its location.

&
Carriage
Dismantling

Probe belt 6. Remove the sub-assembly belt/carriage from

& the rocker module.
Carriage
Dismantling
Probe belt 7. Remove the two (2) fixation screws which

& maintain the belt in the carriage gripper.
Carriage
Dismantling

Probe belt 8. Release the belt from the carriage gripper.

&
Carriage
Dismantling
Probe belt 9. At this stage of the instruction, it is possible

& to change either the belt or carriage.
Carriage
Dismantling

1.1 Belt and probe carriage assembling
 Torx T08
 Torx T20
 Cutting pliers
 Beak pliers
Removal
Action Action Action
1. Follow the instruction of Probe belt & probe 1. Refer to the instruction.
Prerequisite carriage dismantling.
Probe 2 Install the probe carriage on the belt.

Belt &
Carriage
Assembling

Probe 1. Secure the assy. with the two (2) fixation

Belt & screws.
Carriage
2 1
Assembling
Screw to the
mechanical
NOTE : There is no specific torque, screw until stop

the mechanical contact with the probe carriage
then stop screwing.
GOOD
BAD
NOTE: Do not overtight the screws, it could
damage the gripper of the carriage.
Gripper
Is bent
Probe 2. Grease the groove of the rocker.

Belt &
Carriage NOTE: The groove allows the straight driving of
Assembling the probe carriage during the up and down
movements, it must be greased.

Probe 3. Install the assembly Belt/carriage.

Belt &
Carriage
Assembling
Probe 4. Install the probe carriage in the groove.

Belt &
Carriage
Assembling
Probe 2. Install the down pulley in the belt tension

Belt & system.
Carriage
Assembling
Probe 3. Secure the assembly with the fixation screw 1

Belt & to maintain elements together.
Carriage
Assembling

Probe 4. Place the square nut in position and screw by

Belt & few turns.
Carriage
Assembling
Probe 5. Install the metallic shaft inside the rocker.

Belt &
Carriage
Assembling
NOTE: Inserted the shaft first in the dedicated

hole located on the top of the rocker.
Then in the hole of the probe carriage.
Finally, in the bottom of the probe carriage.

Probe 6. Secure the shaft with the fixation screw.

Belt &
Carriage NOTE: Move the washer onto the shaft direction
Assembling before tightening to the dedicated torque.
TORQUE = 80 cNm
Completion 1. Follow the instruction of the Rocker

assembling.
3 Probe’s sensor board replacement

3.1 Probe’s sensor board dismantling
Dismantling
Prerequisite 1. Follow the instruction of Rocker dismantling. 1. Refer to the instruction.
Probe’s 1. Remove the two (2) fixation screws of the

Sensor probe sensor board.
Board
Dismantling

Probe’s 2. Remove the probe sensor board from the

Sensor rocker.
Board
Dismantling

3.2 Probe’s sensor board assembling
Assembling
Prerequisite 1. Follow the instruction of Probe’s sensor 1. Refer to the instruction.

board dismantling
Probe’s 1. Install the probe sensor board on the rocker

1
Sensor with the two (2) fixation screws tighten to
Board the dedicated torque
Assembling
2
TORQUE = 80 cNm
Probe’s 2. Connect the cable to the probe sensor board.

Sensor
Board
Assembling
Probe’s 3. Place a plastic fastener to maintain the cable

Sensor to the board
Board
Assembling

Completion 2. Follow the instruction of the Rocker 1. Refer to the instruction.

assembling.
4 Probe stepper motor replacement

4.1 Probe stepper motor dismantling
Dismantling
Prerequisite 1. Follow the instruction of Rocker dismantling. 1. Refer to the instruction.
Probe 1. From fluidic modules side, locate the two (2)

Stepper fixation screws of the sampling probe motor.
Motor 2
Dismantling

Probe 2. Remove the two (2) fixation screws of the 2

Stepper sampling probe motor.
Motor
Dismantling NOTE: Hold the motor while fixation screws
removing.
Probe 3. From tubing side, disconnect the sampling

Stepper probe motor connector.
Motor
Dismantling 4. Remove the sampling probe motor from the
unit.
4.2 Probe stepper motor assembling
Assembling
Prerequisite 1. Follow the instruction of the Probe stepper 2. Refer to the instruction.
motor dismantling.
Probe 1. Connect the cable to the stepper motor.

Stepper
Motor
Assembling
1

Probe 2. Install the stepper motor in position.

Stepper
Motor
Assembling NOTE: Connector must be oriented through the
top of the unit.
Probe 3. Secure the motor with the two (2) fixation

Stepper screws tighten to the dedicated torque.
Motor
Assembling
1
2
TORQUE = 120 cNm
Completion 1. Follow the instruction of the rocker 1. Refer to the instruction.

assembling.

5 Rocker stepper motor replacement

5.1 Rocker stepper motor dismantling
 Torx T10
 Cutting pliers
 Beak pliers
Dismantling
power supply.
Rocker 1. Place the rocker in central position.

Stepper
Motor
Dismantling
2. Remove the two (2) fixation screws of the

rocker guide.
NOTE: protection can be installed to avoid

screws falling in counter chambers.


Rocker 3. Release (do not remove) the higher fixation

Stepper screw of the holder plate.
Motor
Dismantling
4. Remove the lower fixation screw of the

holder plate.
Rocker 5. Disengage the rocker from its location.

Stepper
Motor
Dismantling
Rocker 6. Place the rocker as shown on the right.

Stepper
Motor
Dismantling

Rocker 1. From fluidic side, remove the two (2) fixation

Stepper screws of the rocker motor.
Motor
Dismantling 2
NOTE: Hold the motor while fixation screws

removal
Rocker 2. From tubing side, disconnect the rocker

Stepper motor connector and remove the rocker
Motor motor from the unit.
Dismantling

5.2 Rocker stepper motor assembling
 Torx T10
Assembling
Prerequisite 1. Follow the instruction of Rocker stepper 3. Refer to the instruction.

motor dismantling.
Rocker 1. Connect the cable to the stepper motor.

Stepper
Motor
Assembling
2. Install the stepper motor in position.
NOTE: Connector must be oriented through the

front of the unit.
3. Secure the motor with the two (2) fixation.
2
NOTE: Do not tighten at this stage.

Rocker 1. Follow the instruction of the rocker 1. Refer to the instruction.

Stepper assembling.
Motor
Assembling
Adjustment 1. Once the motor in position push it up until

the mechanical stop against the rocker gear.
NOTE: Do not push up the motor too much, the

goal is to engage the teeth of motor and rocker
gears each together.
Adjustment 1. Tighten the two (2) screws to the dedicated

torque.
Torque = 120 cNm

6 Rocker optical sensor replacement

6.1 Rocker optical sensor dismantling
 Torx T10
Tools/Material
 Torx T20
Dismantling
Prerequisite 1. Switch off the instrument and disconnect 1. Refer to the instruction.
the power supply.
3. Remove the top cover 3. Refer to the instruction.
4. Remove the Reagent plate. 4. Refer to the instruction.
Rocker 1. Locate the rocker optical sensor on

Sensor tubing side.
Dismantling
Rocker 1. Remove the rocker sensor unscrewing

Sensor the two (2) fixation screws.
Dismantling
1

NOTE: Fixation screws are equipped with

plastic washer.
Rocker 2. Disconnect the sensor connector.

Sensor
Dismantling
Rocker 3. Remove the sensor from the unit.

Sensor
Dismantling


6.2 Rocker optical sensor assembling
 Torx T10
Tools/Material
 Torx T20
Assembling
Prerequisite 1. Follow the instruction of Rocker optical 1. Refer to the instruction.

sensor disassembling.
Rocker 1. Install the sensor in position.

Sensor
Assembling
Rocker 2. Secure the sensor with the fixation screws

1
Sensor tighten to the dedicated torque.
Assembling
2
Rocker 3. Connect the sensor connector.

Sensor
Assembling
Completion 2. Follow the instruction of the rocker 2. Refer to the instruction.

assembling.

Syringe Valves Module #1 Disassembly
The following instructions will guide you to dismantle and assemble the Syringe Valves module #1.
Syringe valves (from syringe Valves Module #1) replacement.
Removal
Prerequisite 7. Drain the instrument from any liquids. 7. Remove pick up tubings from
their containers and select
REAGENT/ PRIME ALL.
main power cord.
Syringe NOTE: Syringe valve module #1 is composed with 2

Valves valves 3/2-1.6mm-M2.5 .
Disassembling
7. Disconnect the valve #18 connector.
V18 V12
NOTE: As an example, the following instruction will

describe the way how to replace the valve #18,
proceed as the same in case of change of valve
N°12.
8. Remove the two fixation screws of the valve

solenoid.

9. Remove the solenoid from the valve body.

body.
11. Remove the valve body from the support.
12. Install the new valve following the reverse

instruction.
NOTE: Be careful to the mounting direction of the

valve body.
The inscription must be oriented through the right
for the two valves which compose the module.
4. Tighten the two fixation screws of the valve

body, respecting a torque to 40cNm.

40cNm
5. Install the valve solenoid on the valve body.

valve solenoid. Connector must be oriented
through the top.

solenoid, respecting a torque to 25cNm.
25cNm
Syringe Valves module #1 replacement.

Syringe Valves module #1 dismantling.
Prerequisite 59. Drain the instrument from any liquids. 51. Remove pick up tubings from their
containers and select REAGENT/ PRIME
ALL.
power supply. 53. Refer to the instruction.
64. Remove the reagent plate
Dismantling 65. Disconnect the six tubings from the assembly.

Dismantling 66. Disconnect the valves connectors.
V18 V12
Dismantling 67. Remove the two fixation screws.

68. Remove the valves assembly from the unit.
V18 V12

Syringe Valves module #1 assembling
Prerequisite 18. Follow the instruction of Syringe Valves 14. Refer to the instruction.
module #1 dismantling.
.
1. Install the valve assembly.

Assembling 2. Secure the assembly with the two fixation
screws tighten to the dedicated torque.
100 cNm
V18 V12
Assembling 3. Connect the six tubings to the assembly.

2. Install the top cover.
5. Connect the power supply and Switch on the 4. Refer to the instruction.
instrument. 5. Refer to the instruction.
6. Perform a prime all cycle. 6. Refer to the instruction.

Syringe Valves Module #2 Module Disassembly
Syringe valves (from syringe Valves Module #2) replacement.
Removal
Prerequisite 13. Drain the instrument from any liquids. 13. Remove pick up tubings from
their containers and select
REAGENT/ PRIME ALL.
main power cord.
Syringe NOTE: Syringe valve module #2 is composed with 2

Valves valves 3/2-1.6mm-M2.5
Disassembling
13. Disconnect the valve #6 connector.
V6 V5
NOTE: As an example, the following instruction will

describe the way how to replace the valve #6,
proceed as the same in case of change of valve N°5.

solenoid.
15. Remove the solenoid from the valve body.


body.
17. Remove the valve body from the support.
18. Install the new valve following the reverse

instruction.

valve body.
The inscription must be oriented through the right
for the two valves which compose the module.

body, respecting a torque to 40cNm.
8. Install the valve solenoid on the valve body.

valve solenoid. Connector must be oriented
through the down.


solenoid, respecting a torque to 25cNm.
40cNm
25cNm

ALL .
power supply.

Dismantling 69. Disconnect the six tubings from the assembly.
V6 V5
Dismantling 71. Remove the two fixation screws.

72. Remove the valves assembly from the unit.
V18 V12

module #2.
4. Install the valve assembly.

Assembling
5. Secure the assembly with the two fixation
100 cNm
V6 V5
Assembling 6. Connect the six tubings to the assembly.

instrument.

Syringe Valves module #3 Disassembly

ALL.
power supply.
Dismantling 73. Disconnect the tubings from the module.

Dismantling 75. Remove the four fixation screws.
76. Remove the Syringe Valves module from the

unit.

module #3.
7. Install the Syringe Valves module.

Assembling 8. Secure the assembly with the four fixation
100 cNm
Assembling 9. Connect the tubings to the assembly.
14. Install the top cover.
instrument. 17. Refer to the instruction.

Fluidic system

Cleaner Line.
1. Cleaner reagent is used to:
 Deproteinize the counting apertures at each analysis cycle avoiding clogging.

 Deproteinize the counting chambers and the counting apertures, when shut down
selected.
2. Cleaner Line.
3. Cleaner line is composed specifically with 1 valves 2/2.

V11: <OFF> Cleaner line is closed.
<ON> Cleaner line is opened.

4. Cleaner prime.
NOTE: Because there is no dedicated syringe for cleaner, system will use valves #8 and #10 and waste
syringe to prime the cleaner solution.
 Valves #11, #8, #10 <ON>.

 Syringe motor moves pistons down.
 Cleaner is aspirated inside the waste syringe.

5. Cleaner into apertures (backflush)
 Cleaner is already primed at the back of the apertures.

 Syringe motor moves pistons up.
 Valves #4, #12 <ON>
 Thanks to diluent line, cleaner is pushed through apertures.
NOTE: Typically, this case is used at the end of each analysis cycle to perform a backflush into the
apertures.

6. Cleaner into counting chambers.
6.1 Cleaner Prime.
 Valves #11, #8, #10 <ON>.


6.2 WBC bath filled with Cleaner

 Valves #1 <ON>

6.3 Cleaner Prime.
 Valves #11, #8, #10 <ON>.


6.4 RBC bath filled with Cleaner

 Valves #2 is ON

Fluidic Diluent Line.

7. Diluent reagent is used to:
 Perform the dilutions WBC and RBC.

 Make the sheath in the optic flow cell.
 Wash the needle internally and externally.
 Rinse the needle externally.
 Be present at the back of the apertures during the counting.
8. General Diluent Line.

9. Diluent line is composed with 6 valves 3/3.
V4: Aspiration of the diluent into the syringe. V18: Dispense diluent to V12.
Dispense diluent to V5. Dispense diluent to RBC bath
V5: Dispense diluent to V6. V12: Dispense diluent to rinse head.
Dispense diluent to internal needle. Dispense diluent to V15
V6: Dispense diluent to V18. V15: Dispense diluent to apertures.
Dispense diluent to WBC bath Dispense diluent to flow cell (sheath)

10. Diluent aspiration in diluent syringe.
 All valves in normal state, as shown in the drawing.

 Due to the normal state of valve #4, diluent is aspirated inside the diluent syringe.

11. Diluent to the rinse head.
 Valve #4 is ON.
 Diluent is pushed through V4/V5/V6/V18/V12/Rinse head.
NOTE: Typically, this case is used to wash and to rinse the needle externally.
Needle washing: (1) The diluent is pushed to the rinse head (2) at the same time vacuum is applied at the
opposite side of the rinse head (3) the needle moves from down to up.
Needle rinsing: (1) The diluent is pushed to the rinse head without vacuum applied on the opposite side.
a) Diluent fall down along the needle and rinse it

12. Diluent to the internal of the needle.
 Valve #4 ON
 Valve #5 ON
 Diluent is pushed through V4/V5/Sampling syringe/Internal needle.
NOTE: Typically, this case is used to dispense the blood into the counting chambers, to perform 1/3 of the
WBC and RBC dilution and to rinse the needle internally.
13. Diluent to the WBC bath.


 Valve #4 ON
 Valve #6 ON
 Diluent is pushed through V4/V5/V6/heating system/WBC bath.
NOTE: Typically, this case is used to perform 2/3 of the WBC dilution.

14. Diluent to the RBC bath.
 Valve #4 ON
 Valve #18 ON
 Diluent is pushed through V4/V5/V6/V18/heating system/RBC bath.
NOTE: Typically, this case is used to perform 2/3 of the RBC dilution.

15. Diluent to flow cell.
 Valve #4 ON
 Valve #12 ON
 Valve #15 ON
 Diluent is pushed through V4/V5/V6/V18/V12/V15/Flow cell.
NOTE: Typically, this case is used to make the diluent sheath in the flow cell during optic measurement.

16. Diluent to the apertures.
 Valve #4 ON
 Valve #12 ON
 Diluent is pushed through V4/V5/V6/V18/V12/V15/apertures.
NOTE: Typically, this case is used to supply the fluidic located at the back of the apertures with diluent.

Lyse Supply Line

Lyse Supply to Optical bench

Valve 14 “OFF”
Valve 9 “ON”

Lyse Supply to WBC bath
Valve 9 “ON”
Valve 14 “ON”

Lyse suction to syringe

Fluidic Waste Line.
17. Waste line is used to:
 Drain WBC counting chamber.

 Drain RBC counting chamber.
 Drain optical flowcell during measurement.
 Drain rinse head during needle cleaning.
 Drain waste syringe.
18. General Waste Line.

19. Waste line is composed with 4 valves.
V3: Drain rinse head during needle cleaning

V1: Open WBC bath for draining
V7: Drain waste syringe, when pistons move

V2: Open RBC bath for draining
up
20. Drain WBC counting chamber.
 Valve #1 switched on.


Thanks to the vacuum created in waste syringes when pistons go down and valve #1 opened, the liquid
present in WBC chamber will be drained to the waste syringes.
21. Drain RBC counting chamber.

Thanks to the vacuum created in waste syringes when pistons go down and valve #2 opened, the liquid
present in RBC chamber will be drained to the waste syringes.

22. Drain waste syringe

Thanks to the pistons moving up and Valve#7 opened, the liquid present in waste syringe is
evacuated to the waste container.

23. Drain optical flowcell during measurement.

During optic measurement diluent and sampling dilution are pushed through the nozzle and naturally
evacuated to the waste container.

24. Drain rinse head during needle cleaning.
 Valve #3 in normal state.

 Pump is ON.
Thanks to the pump ON and valve #3 in normal state, liquid is aspirated from rinse head and evacuated to
the waste container.

MISPACOUNTPLUS Sampling
All valves in normal state, pistons move down.
When sampling piston moves down it allows the blood aspiration inside the needle

Optic back flush
Valve #16 is switched on and pistons move down allowing.

RBC bubbling
1. Valve #17 and #8 are switched on, pistons move down.

 Air is aspirated in waste syringe.

2. Valve #2 is switched on and piston move up.

 Air is pushed in RBC chamber making the bubbling.

WBC bubbling
1. Valve #17 and #8 are switched on, pistons move down.

 Air is aspirated in waste syringe.

2. Valve #1 is switched on and piston move up.

 Air is pushed in WBC chamber making the bubbling.

Mispa Count Plus Service Manual

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Mispa Count Plus Service Manual

Uploaded by

Copyright:

Available Formats

DOC No.

DOCUMENT NAME- Service manual

EQUIPMENT MODEL- Mispa Count Plus

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 1

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 2

Chapter 1 Safety Guidance……………………………………………………………………………………………………………………………………….…8

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 3

6.8.1 Graphical User Interface…………………………………………………………………………………………..………43

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 4

9.7.2 BLEACH CLEANING………………………………………………………………………………78

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 5

Rinse pump assembling…………………………………………………………………………………………………………183

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 6

3 Probe’s sensor board replacement……………………………………………………………………………..…….269

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 7

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 8

Chapter 1 Safety Guidance

1.1 Symbols and Definitions

WARNING RISK OF DANGER. Indicates a procedure to be strictly respected in order to

1.2 Warnings and Cautions

Check if the voltage setting matches the supply voltage.

YOUR BEST PARTNER IN DIAGNOSTICS 9

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 10

Chapter 2 General Overview

White blood cell parameters:

Red blood cell parameters:

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 11

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 12

2.2 Main Parts

2.2.1 Front cover

1. Touch screen LCD display (8.4”).

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 13

2.2.2 Fluidic part

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 14

2.2.3 Reagent area

2.2.4 Connection board

Serial Link RS232 Port

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 15

2.2.5 External power supply block

2.2.6 Printer (optional)TBD

2.2.7 External Barcode reader (optional)

 Sample identification SID.

AGAPPE DIAGNOSTICS LTD

YOUR BEST PARTNER IN DIAGNOSTICS 16

 1 printer with USB connection

Chapter 3 Installation Guidance

3.1 Installation Requirement

3.1.2 Installation environment

 Maximum relative humidity for temperatures up to 31 °C is 80 %, decreasing linearly to 50 % at 40 °C.

AGAPPE DIAGNOSTICS LTD