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Received: 10 December 2021 Accepted: 7 April 2022

DOI: 10.1111/jopr.13526

ORIGINAL ARTICLE

Influence of the Number of Teeth and Location of the Virtual


Occlusal Record on the Accuracy of the Maxillo-Mandibular
Relationship Obtained by Using An Intraoral Scanner

Marta Revilla-León, DDS, MSD, PhD1,2,3 Jorge Alonso Pérez-Barquero, DDS4


Álvaro Zubizarreta-Macho, DMD, PhD5 Abdul B. Barmak, MD, MSc, EdD6
Wael Att, DDS, Dr Med Dent, PhD7 John C. Kois, DDS, MSD1,2,8

1
Department of Restorative Dentistry, School of Abstract
Dentistry, University of Washington, Seattle, WA
Purpose: To assess the influence of the number of teeth (2, 3, or 4) and location (molars,
2
Kois Center, Seattle, WA molar and premolar, or premolars and canines) of the bilateral virtual occlusal record
3
Revilla Research Center, Madrid, Spain on the accuracy of the virtual maxillo-mandibular relationship acquired by an intraoral
4
Department of Dental Medicine, Faculty of scanner (IOS).
Medicine and Dentistry, University of Valencia, Material and methods: Diagnostic casts mounted on a semi-adjustable articulator
Valencia, Spain
were obtained. Four markers were adhered on the facial surfaces of the first molars
5
Department of Implant Surgery, Faculty of and canines. The mounted casts were digitized using an extraoral scanner. Maxillary
Health Sciences, Alfonso X El Sabio University,
Madrid, Spain
and mandibular intraoral digital scans were obtained using an intraoral scanner (TRIOS
6
4). The maxillary and mandibular digital scans were duplicated 105 times and divided
Eastman Institute of Oral Health, University of
Rochester Medical Center, Rochester, NY into 7 groups based on the number of teeth (2, 3, or 4) and location (molar, molar and
7
premolar, or premolars and canines) of the bilateral virtual occlusal records (n = 15).
Department of Prosthodontics, Tuff University
School of Dental Medicine, Boston, MA The alignment of the scans was automatically performed after the acquisition of the
8
Private Practice, Seattle, WA
corresponding occlusal records by the IOS program. Eight linear distances between the
gauge balls were computed on the reference scan and on the 105 digital scans. The
Correspondence distances obtained on the reference scan were used to calculate the discrepancies with
Marta Revilla-León, DDS, MSD, PhD, 1001 the distances obtained on each experimental scan. The Shapiro-Wilk test showed that
Fairview Ave N # 2200, Seattle, WA 98109.
the data was normally distributed. The trueness and precision data were analyzed using
E-mail: marta@koiscenter.com
2-way ANOVA followed by pairwise comparison Tukey tests (α = 0.05).
Funding information Results: Two-way ANOVA showed that the number of teeth (p < 0.001) and the posi-
This research did not receive any specific grant tion of the virtual occlusal record (p < 0.001) were significant factors on the accuracy of
from funding agencies in the public, commercial, the maxillo-mandibular relationship. Tukey test showed significant overall mean differ-
or not-for-profit sectors.
ences between the different groups tested: the 4-teeth group obtained the highest true-
ness, and the 2-teeth group showed the lowest trueness values (p < 0.001). Tukey test
showed significant trueness differences between the virtual occlusal record locations.
The 2-teeth record located more posteriorly obtained the lowest trueness. Significant
differences in precision values were found among the subgroups tested (p < 0.001).
The 2-teeth group obtained significantly more precision values than the 3- and 4-teeth
groups. Additionally, there was a significant difference in precision values between the
subgroup tested in which the first molar and second premolar location had the highest
precision, while the first and second premolar’s location obtained the lowest precision.
Conclusions: The number of teeth and the location of the bilateral virtual occlusal
record influenced the accuracy of the virtual maxillo-mandibular relationship obtained
by the intraoral scanner tested. The more teeth included in the bilateral virtual occlusal
record, the higher the accuracy of the maxillo-mandibular relationship. Additionally,

J. Prosthodont. 2023;32:253–258. wileyonlinelibrary.com/journal/jopr © 2022 by the American College of Prosthodontists. 253


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254 REVILLA-LEÓN ET AL

the more anteriorly located the virtual bilateral occlusal record involving 2 or 3 teeth
was, the higher the accuracy mean value.

KEYWORDS
Intraoral digital scan, virtual occlusal records, virtual articulator, virtual patient

Digital occlusal records obtained by using intraoral scan- right canines (Fig 1). Then, the mounted diagnostic casts
ners (IOSs) are an integral part of the digital workflow for were digitized by using a laboratory dental scanner (Dental
fabricating dental prostheses. Dental literature has analyzed 3D Scanner Medit T500; Identica) following the manufac-
the accuracy of the virtual interocclusal records reporting turer’s recommendations. The manufacturer of the laboratory
an adequate reproduction of the maxillo-mandibular occlusal scanner used reports a scanning accuracy of <7 μm following
contacts.1–12 However, differing characteristics of the vir- the ISO 12836.
tual occlusal records such as the position in the dental arch, Maxillary and mandibular intraoral digital scans were
dimensions, and number the occlusal record sections, and the obtained using an IOS (TRIOS 4, wireless, v. 21.2.0; 3Shape
extension (quadrant or complete-arch) of the intraoral digital A/S) following the scanning protocol endorsed by the man-
scans can affect the maxillo-mandibular relationship of the ufacturer. The IOS device was calibrated before starting and
virtual diagnostic casts mounted on the virtual articulator.5,10 after every 10 scans following the manufacturer’s protocol.14
A previous in vitro study showed that the combination of Furthermore, the intraoral digital scans were acquired under
bilateral virtual occlusal records with a dimension of 10 × 1000-lux illumination conditions (LX1330B Light Meter; Dr.
15 mm obtained a better 3-dimensional (3D) spatial position Meter Digital Illuminance) in a room without windows.15,16
of the mandibular cast in reference to its corresponding max- All the digital scans were obtained by a prosthodontist (M.R-
illary cast on a virtual articulator.5 However, the influence of L.) with 11 years of previous experience handling IOSs.
tooth type and number of teeth involved in the bilateral virtual The maxillary and mandibular intraoral digital scans were
occlusal records on accuracy remains unclear. duplicated 105 times and divided into 3 groups based on the
The accuracy is measured by trueness and precision.13 number of teeth involved: 2 (G2 group), 3 (G3 group), or 4
Trueness describes the ability of a digitizer to obtain the teeth (G4 group). Additionally, G2 and G3 groups were sub-
maxillo-mandibular relationship by obtaining a bilateral vir- divided into 3 subgroups depending on the teeth location of
tual occlusal record as close as possible to its real rela- the bilateral virtual occlusal record (Table 1) (n = 15).
tionship, while precision designates the reproducibility of The alignment of the virtual maxillary and mandibular
the maxillo-mandibular relationship by obtaining a bilateral scans was automatically performed after the acquisition of the
virtual occlusal record captured under the same scanning corresponding bilateral virtual occlusal records by the IOS
conditions.13
The purpose of the present in vitro study was to assess the
influence of the number of teeth (2, 3, or 4) and the loca-
tion (molars, molar and premolar, or premolars and canines)
of the bilateral virtual occlusal record obtained by using an
IOS (TRIOS 4, wireless, v. 21.2.0; 3Shape A/S) on the accu-
racy of the maxillo-mandibular relationship on the virtual
articulator. The null hypothesis was that there would be
no difference on the accuracy (trueness and precision) of
maxillo-mandibular relationship on the virtual articulator
obtained from virtual occlusal records acquired with different
number of teeth involved and differing locations.

MATERIAL AND METHODS

Maxillary and mandibular conventional diagnostic stone casts


mounted on a semi-adjustable articulator (Panadent Articula-
tor; Panadent) were obtained. The maxillary and mandibu-
lar diagnostic casts presented with second molar to the
second molar dentition. Subsequently, four 1mm diameter
markers (DentalMark 1.0 mm lead ball; Suremark) were
adhered on each diagnostic cast on the middle third of the F I G U R E 1 Maxillary and mandibular conventional diagnostic stone
buccal surfaces of the left and right first molars and left and cast mounted on a semi-adjustable articulator
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VIRTUAL OCCLUSAL RECORD ACCURACY 255

TA B L E 1 Description of the bilateral virtual occlusal record In the G4 group, the bilateral virtual occlusal record
characteristics obtained on each experimental group involved the maxillary and mandibular right and left first
Location (tooth type and second molars, first and second premolars, and canines
involved) in the maxillary (Fig 2g).
Group Dimensions and mandibular arch The STL files were imported into a reverse engineering
G2-1 2 teeth bilaterally Right and left second molar software program (Geomagic Control X; 3D Systems). As the
Right and left first molar maxillary and mandibular digital scans were already aligned
G2-2 Right and left first molar automatically after obtaining the bilateral virtual occlusal
Right and left second premolar record by the IOS software program, the maxillary and
G2-3 Right and left second premolar mandibular scans were aligned when importing them into the
Right and left first premolar reverse engineering software program. Eight linear distances
G3-1 3 teeth bilaterally Right and left second molar between the centers of the gauge balls were computed on the
Right and left first molar reference scan and on the 105 intraoral digital scans using the
Right and left second premolar same software program (Fig 3). The linear distances obtained
G3-2 Right and left first molar on the reference scan were used to calculate the discrepan-
Right and left first premolar cies with the linear distances obtained on each experimental
Right and left second premolar
intraoral digital scan.
G3-3 Right and left first premolar The Shapiro-Wilk test showed that the data was normally
Right and left second premolar
distributed. The trueness and precision data were analyzed
Right and left canine
using 2-way ANOVA followed by post hoc pairwise multiple
G4 4 teeth bilaterally Right and left first molar
comparison Tukey tests (α = 0.05). All the data was analyzed
Right and left second molar
Right and left first premolar using a statistical software program (SPSS Statistics v25 for
Right and left second premolar Windows; IBM Corp.).
[Correction added on July 4, 2022, after first online publication: For group G3-2, “Right
and left second molar” was changed to “Right and left first molar” in table 1 and under
MATERIAL AND METHODS section] RESULTS

software program. Therefore, no manual alignment was per- The overall mean ±standard deviation (SD) measurement dis-
formed. Additionally, the scanning protocol of the bilateral crepancies (trueness ±precision) are presented in Table 2.
occlusal record recommended by the IOS manufacturer was Regarding the trueness assessment, 2-way ANOVA analy-
followed. The right virtual occlusal record started on the buc- sis of variance showed that the number of teeth involved
cal surface of the most posterior maxillary and mandibular (G1, G2, and G3 groups) (df = 2, MS = 0.0040, F =
teeth, in a horizontal anterior movement; the data acquisi- 255.29, p < 0.001) and the position of the bilateral virtual
tion finished on the more anterior maxillary and mandibular occlusal record (df = 4, MS = 0.0032, F = 203.12, p <
teeth included in the record. The same procedure was com- 0.001) were significant factors on the accuracy of the maxillo-
pleted for the left virtual occlusal record. After the acqui- mandibular virtual relationship. Additionally, the number of
sition of both virtual occlusal records of each group, the teeth involved explains the 36.14% of variation in the accu-
post-processing procedures were completed by using the IOS racy of the maxillo-mandibular virtual relationship, while
software program and the aligned maxillary and mandibular the position of the bilateral virtual occlusal record evalu-
virtual casts were exported in the same x-, y-, and z-axis ori- ated explains the 57.05% of variation in the accuracy of the
entation in standard tessellation language (STL) file format. maxillo-mandibular virtual relationship (Fig 4).
In the G2-1 group, the bilateral virtual occlusal record Tukey post hoc multiple pairwise comparison test showed
involved the maxillary and mandibular right and left second significant overall measurement discrepancies and mean
and first molars (Fig 2a). In the G2-2 group, the bilateral vir- value differences among the different groups tested (G1,
tual occlusal record involved the maxillary and mandibular G2, and G3 groups). All the groups were significantly dif-
right and left first molar and second premolar (Fig 2b). In the ferent from each other. The G4 group obtained the highest
G2-3 group, the bilateral virtual occlusal record involved the trueness compared with the remining groups, while the G2
maxillary and mandibular right and left first and second pre- groups obtained the lowest trueness among the groups. Addi-
molars (Fig 2c). tionally, Tukey post hoc multiple pairwise comparison test
In the G3-1 group, the bilateral virtual occlusal record showed significant overall measurement discrepancies and
involved the maxillary and mandibular right and left first and mean value differences between the bilateral virtual occlusal
second molars and second premolar (Fig 2d). In the G3-2 record location. The subgroup G4 obtained the lowest over-
group, the bilateral virtual occlusal record involved the max- all mean measurement discrepancies compared with the other
illary and mandibular right and left first molar and first and subgroups tested, and the G2-1 subgroup obtained the high-
second premolars (Fig 2e). In the G3-3 group, the bilateral est overall mean measurement discrepancies compared with
virtual occlusal record involved the maxillary and mandibular the other subgroups tested. The more anteriorly the virtual
right and left first and second premolars and canine (Fig 2f). bilateral occlusal recorded in the present study, a higher the
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256 REVILLA-LEÓN ET AL

F I G U R E 2 Representative bilateral virtual occlusal record obtained among the different experimental groups. (a), G2-1 group. (b), G2-2 group. (c),
G2-3 group. (d), G3-1 group. (e), G3-2 group. (f), G3-3 group. (g), G4 group. G2, 2 teeth; G3, 3 teeth; G4, 4 teeth

trueness mean value of the virtual maxillo-mandibular rela- significantly influenced the accuracy of the virtual maxillo-
tionship was obtained. mandibular relationship. Therefore, the null hypothesis was
Regarding the precision evaluation, factorial ANOVA rejected.
showed significant differences in overall mean discrepancy Dental literature has previously evaluated the accuracy
SDs of the three distance groups of teeth and their respec- of virtual maxillo-mandibular relationships established with
tive teeth location subgroups (p < 0.001). Tukey post hoc differing virtual occlusal record characteristics acquired by
test showed that the G2 group had significantly higher pre- using IOSs.1–12 However, no previous study assessed if
cision mean values than the G3 and G4 groups, but no sig- the number of teeth and location of the virtual bilateral
nificant difference was shown between G3 and G4 groups. occlusal record influence the virtual maxillo-mandibular
Additionally, there was a significant difference in precision relationship.1–12 Therefore, comparisons with previous stud-
values between the subgroup tested in which the first molar ies are challenging. This in vitro study obtained higher
and second premolar location had the highest precision, while accuracy on the virtual maxillo-mandibular relationship when
the first and second premolar’s location obtained the lowest 4-teeth were included in the virtual bilateral occlusal record
precision. compared with those that contained only 2 or 3 teeth. This
might be explained by surface information variations among
the 2-, 3-, and 4-teeth virtual occlusal records that the
DISCUSSION IOS software tested uses to establish the virtual maxillo-
mandibular relationship. The greater the amount of sur-
The results of the present study revealed that the number of
face information available on the virtual occlusal record,
teeth and the location of the virtual bilateral occlusal record
the higher the accuracy on the virtual maxillo-mandibular
relationship outcome. Therefore, it might be recommended
to include at least 4 teeth comprising the virtual bilateral
occlusal record when using the selected IOS system. How-
ever, additional studies are recommended to assess the influ-
ence of bilateral occlusal records involving more than 4 teeth
on the accuracy of the virtual maxillo-mandibular relation-
ship captured using IOSs.
In the present study, when the same number of teeth were
involved in the bilateral occlusal record, the anterior loca-
F I G U R E 3 (a), Representative linear inter-landmark measurements tions of those records showed better accuracy compared with
on the right quadrants. (b), Representative linear inter-landmark posterior locations. This might be also explained by a “tri-
measurements on the left quadrants pod” effect during the alignment. The virtual casts might be
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VIRTUAL OCCLUSAL RECORD ACCURACY 257

F I G U R E 4 Trueness evaluation. (a), Boxplot of overall mean measurement discrepancies obtained among the groups tested. (b), Main effects plot for
mean overall measurement discrepancies obtained among the groups tested

TA B L E 2 Trueness and precision mean values computed among the selected for obtaining the reference data, all the groups were
groups tested. Data provided in millimeters (mm) assessed using the same methodology. Comparisons could
Trueness ± precision then be made among the groups, providing valuable data.
(Overall mean ±SD Further laboratory and clinical studies are needed to assess
Group Subgroup measurement discrepancies) the influence of the number of teeth and location of the vir-
G2 (2 teeth) 1 0.098a ± 0.004b tual occlusal records on the accuracy of the virtual maxillo-
2 0.073c ± 0.003d mandibular relationship when different IOS devices and
post-processing procedures are used, and an industrial scan-
3 0.064d ± 0.002b
ner is selected as a reference output. Furthermore, different
G3 (3 teeth) 1 0.079b ± 0.003c
clinical scenarios, such as partial edentulism, might produce
2 0.065d ± 0.002c different outcomes when compared with the results of the
3 0.058e ± 0.006a present study. Moreover, additional studies are recommended
G4 (4 teeth) 0.053f ± 0.002b to assess the accuracy of the maxilla-mandibular relationship
when the alignment of the maxillary and mandibular diagnos-
SD, standard deviation.
Trueness values that do not share a letter are significantly different. tic casts is completed using manual and automatic methods.
Precision values that do not share a letter are significantly different. Additionally, future investigations should evaluate the influ-
ence of the number of teeth and location of the bilateral vir-
tual occlusal record on the accuracy of the virtual maxillo-
better articulated if the surface or contact points contained in mandibular relationship acquired by using extraoral or indus-
the bilateral virtual record are homogenously distributed on trial scanners.
the arches. A previous in vitro study assessed the influence of the
In the present study, the research methodology aimed number and size of the virtual occlusal records on the vir-
to minimize the variables that could influence the vir- tual maxillo-mandibular relationship.5 Authors compared 3
tual maxillo-mandibular relationship outcome. The intraoral possible locations (frontal, left lateral, and right lateral)
digital scans of the maxillary and mandibular casts were and 2 differing dimensions (10 × 15 and 25 × 15 mm)
duplicated, avoiding discrepancies caused by variability of the occlusal records. The assessment was completed by
introduced by the intraoral digitalization of the casts. How- comparing the existing physical occlusal contacts and the
ever, it is important to remember that the virtual maxillary and determined virtual contacts. The results revealed that the
mandibular scans used and duplicated were obtained using an combination of bilateral virtual occlusal records with a
IOS device which might also have introduced scanning dis- dimension of 10 × 15 mm obtained the highest accuracy on
crepancies on the virtual casts and influenced on the results the virtual maxillo-mandibular relationship.4 The 10 × 15 and
obtained. 25 × 15 mm might correspond with the 2- and 3-teeth groups
Similarly, the virtual relationship between the digital of the present study, respectively. Contrary to the results of
maxillary and mandibular diagnostic casts was established the present study, the authors reported better accuracy when
automatically by the IOS software, without any opera- using the shortest mesio-distal occlusal record (10 × 15 mm).
tor intervention. Although a laboratory dental scanner was However, the virtual occlusal records were obtained with an
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258 REVILLA-LEÓN ET AL

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CONFLICT OF INTEREST
The authors did not have any conflict of interest, financial or
personal, in any of the materials described in this study. How to cite this article: Revilla-León M, Alonso
Pérez-Barquero J, Zubizarreta-Macho Á, Barmak AB,
ORCID Att W, Kois JC. Influence of the Number of Teeth and
Marta Revilla-León, DDS, MSD, PhD https://orcid.org/ Location of the Virtual Occlusal Record on the
0000-0003-2854-1135 Accuracy of the Maxillo-Mandibular Relationship
Wael Att, DDS, Dr Med Dent, PhD https://orcid.org/0000- Obtained by Using An Intraoral Scanner. J
0002-4879-0895 Prosthodont. 2023;32:253–258.
John C. Kois, DDS, MSD https://orcid.org/0000-0002- https://doi.org/10.1111/jopr.13526
5022-7567

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