Artificial insemination (AI)

Ziyad Al-Kass
E-mail:ziyadalkass@gmail.com
 Artificial insemination (AI)
• Collecting sperm cells from a male animal.
• Manually depositing sperm into the reproductive tract of a female.
Advantages:
1. Increase efficiency of bull usage
2. Increase potential for genetic selection
3. Decrease costs
4. Increase safety for animals and farmers
5. Reduce disease transmission
Disadvantages:

Your Job
 Artificial insemination technique
• Preparations for insemination • General tips
❖ Insert the insemination gun upward at a 30 ̊ to 40 ̊
1. Must the cow to be bred is in heat. angle ( to avoid entering the urethral opening on the
2. Hold or fix the cow good. floor of the vagina).
❖ The inseminator should be able to feel the gun within
3. Inseminator prepare him or her self the vaginal fold, but unable within the cervix (he or
(clothes, cloves ect,,,,,). she able to recognizing the correct position of gun).
❖ The insemination must be in the uterine body, is quite
small.
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Artificial insemination Methods
1. Recto-vaginal method
• Most use in large animals e.g Cattle.
• The semen loading in insemination gun.
• The inseminator insert one hand in rectum.
• the hand inside rectum hold the cervix.
• The A.I gun insert into vagina and direction by other hand.
• Semen deposit in uterine body.
• after depositing the semen the gun is remove.
 2. Spectrum method
• Spectrum is place in the vagina.
• Provides passage to the site of
insemination.
• Inseminating tube is passed through the
spectrum.
• Semen is deposit at the cervix.
 3. Vaginal method
• Semen is deposit in the vagina, as close as possible to the entrance of the cervix.
• Using mostly in small animals.
• The ewe should be position with the rear elevate at a 45° angle.
• The technician uses a vaginal speculum and a light source to visualize the
entrance of the cervix.
• The inseminating dose for this technique is 200 to 400 x 106 spermatozoa and
the volume of 0.2mL.
 4. Laparoscopic Insemination
• Semen is deposit directly in the uterine horn of the female.
• The procedure is surgical.
• Using in ewes or goats.
• Animals fasting for at least 12 hours before.
•
4. Laparoscopic Insemination
Pregnancy rate after A.I. in sheep
Sperm A.I. Method Pregnancy rate
Frozen Superficial cervix 32.2%
deep cervix 44.4%
laparoscopic 57.1%
Refrigerated Vaginal 35.7%
Laparoscopic 71.8%
Semen transportation

chilled semen transport box

Frozen semen
References:;
1. Mike, O’, C. and Jana, P. Reviewing Artificial Insemination Technique. Penn State’s
College of Agricultural Sciences
2. Reproduction Biotechnology in Farm Animals. Isbn: 978-93-86337-64-1