BIOTECHNOLOGY PRINCIPLES AND PROCESSES

HHH

GOLDEN PRACTICE SHEETS

1. Restriction endonucleases: - 7. Electroporation procedure involves :-

(1) Are synthesized by bacteria as part of their defence (1) Fast passage of food through sieve pores in phloem
mechanism elements with the help of electric stimulation.
(2) Are present in mammalian cells for degradation of (2) Opening of stomatal pores during night by
DNA when the cell dies artificial light
(3) Are used in genetic engineering for ligating two DNA (3) Making transient pores in the cell
molecules membrane to introduce gene constructs
(4) Are used for invitro DNA synthesis (4) Purification of saline water with the help of a
membrane system.
2. Agrobacterium tumefaciens contains a large
plasmid, which induces tumour in the plants it is 8. A suitable vector for gene cloning in higher
termed as :- organism is :-
(1) Ti plasmid (1) Baculovirus
(2) Ri plasmid (2) Retrovirus
(3) Recombinant plasmid (3) Salmonella typhimurium
(4) Shine Delgrano sequence (4) Neurospora crassa

3. In which type of bioreactor air bubbles 9. In the following diagram showing a

dramatically increases the oxygen transfer area? typical agaro gel electrophoresis
(1) Simple stirred tank bioreactor 1. Lane 1 shows undigested DNA fragment
2. Lane 4 shows largest DNA particles towards
(2) Sparged stirred tank bioreactor
‘y’
(3) Both (1) & (2)
3. The procedure helps to form clones of DNA
(4) None of these 4. The procedure takes place as the DNA
fragments are double
4. Which of following feature is not necessary for stranded
cloning vector?
(1) Origin of replication
(2) High copy number
(3) Selectable marker
(4) Cloning sites

5. The substrate for restriction enzyme is :-

(1) Single stranded RNA
(2) Proteins
(3) Double stranded DNA 10. Select the correct statement .
(4) Single stranded DNA 1. A restriction exonuclease cuts both strands of
foreign DNA as well as vector DNA at specific
6. Taq - polymerase which is used for amplification palindromic sequences
of DNA related with: - 2. A restriction endonuclease is named on the
basis of scientific name and strain of bacteria
(1) Hybridoma technique
from which it is isolated
(2) PCR-technique
3. All restriction enzymes cut the strands of
(3) Gene cloning DNA in the centre of palindromic site between
(4) r-DNA technology the same two bases of opposite strand

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4. When cut by same restriction enzyme, the resultant
DNA fragments generate different kinds of sticky ends
11. During the process of gel electrophoresis, DNA
moves towards the _A_ of the electrophoretic
chamber as the charge of DNA is _B_.
Choose the option that fills the blanks correctly.
A B
1. Cathode Positive
2. Cathode Negative
3. Anode Positive
4. Anode Negative
12. Read the following statements
Statement-A : Restriction enzymes are
obtained only from eukaryotes.
Statement-B : The first discovered restriction
endonuclease was EcoRI.
Choose the correct option.
(1) Both statements are correct
(2) Both statements are incorrect
(3) Statement A is correct but B is incorrect
(4) Statement B is correct but A is incorrect
13. Mark the correct statement for pBR322 as a
plasmid vector
I. Contains relaxed origin of replication.
II. Two genes coding for resistance to antibiotics have
been introduced.
III. There are single recognition sites for a number of
restriction enzymes at various points.
IV. Insertional inactivation is a useful selection method
for identifying recombinant vectors with insert.
1. I, II, III & IV
2. I, II only
3. II, III only
4. II only
14. Given below are four statements pertaining to
separation of DNA fragments using gel
electrophoresis. Identify the incorrect statements.
(i) DNA is negatively charged molecule and so it is loaded
on gel towards the anode terminal.
(ii) DNA fragments travel along the surface of the gel
whose concentration does not affect movement of DNA.
(iii)Smaller the size of DNA fragment larger is the distance
it travels through it.
(iv) Pure DNA can be visualized directly by exposing UV
radiation.
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Choose correct answer from the options given
below.
(a) (i), (iii) and (iv)
(b) (i), (ii) and (iii)
(c) (ii), (iii) and (iv)
(d) (i), (ii) and (iv)
15. Which of the following statements is
incorrect with respect to polymerase chain
reaction?
(1) Repeated amplification is achieved by the
use of thermostable DNA polymerase
(2) Thermostable DNA polymerase is isolated
from a bacterium, Thermus aquaticus
(3) Each cycle in PCR has three steps, first is
primer annealing, second is denaturation and
third is extension of primers
(4) In polymerase chain reaction, multiple
copies of gene of interest are synthesized in
vitro using two sets of primers and enzyme DNA
polymerase
16. Which statement regarding restriction
endonucleases is NOT correct?
1. They recognize a specific base sequence in
the DNA.
2. They are produced by bacterial cells as a
primitive immune system.
3. They digest DNA by removing nucleotides
from a free 3' end.
4. They often generate stick ends.
17. Assertion (A) : β-galactosidase coding
sequence acts as a selectable marker.
Reason (R) : Enzyme galactosidase
converts the galactose into lactose.
A. Both assertion (A) and reason (R) are true and
reason (R) is the correct explanation of assertion
(A).
B. Both assertion (A) and reason (R) are true
but reason (R) is not the correct explanation
of assertion (A).
C. Assertion (A) is true but reason (R) is false.
D. Assertion (A) is false but reason (R) istrue.
18. Assertion (A) : DNA is a positively
charged molecule.
Reason (R) : DNA moves towards the
positive electrode (anode).
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A. Both assertion (A) and reason (R) are true and
reason (R) is the correct explanation of assertion
(A).
B. Both assertion (A) and reason (R) are true but
reason (R) is not the correct explanation of
assertion (A).
C. Assertion (A) is true but reason (R) is false.
D. Assertion (A) is false but reason (R) is true. (1) A - Exonuclease cuts the DNA between bases
G and A only when the sequence GAATTC is
present in the DNA
19. Which one of the following techniques made it (2) B - Sticky ends are formed.
possible to genetically engineer living organisms ? (3) C - The DNA fragments join at sticky ends by
1. Hybridization DNA ligase.
2. Recombinant DNA techniques (4) D - Restriction enzyme Eco RI cuts the strand
3. X-ray diffraction of DNA a little away from centre of the
4. Heavier isotope labelling palindromic sites, but between the same two
bases on the opposite strands
20. Choose the correct pair from the following.
(1) Ligases - Join the two DNA molecules 24. The bioreactor shown in the diagram
(2) Polymerases - Break the DNA into fragments is:
(3) Nucleases - Separate the two strands of
DNA
(4)Exonucleases - Make cuts at specific positions
within DNA

21. Following statements describe the

characteristics of
the enzyme restriction endonuclease. Identify the
incorrect statement.
(1) The enzyme recognises a specific palindromic
nucleotide sequence in the DNA.
(2) The enzyme cuts DNA molecule at identified
position within the DNA.
(3) The enzyme binds DNA at specific sites and
cuts only one of the two strands.
(4) The enzyme cuts the sugar-phosphate backbone
at specific sites on each strand.
22.The correct order of steps in Polymerase Chain
Reaction (PCR) is
(1) extension, denaturation, annealing
(2) annealing, extension, denaturation
(3) denaturation, extension, annealing
(4) denaturation, annealing, extension.
23. Following are the steps in the formation of
recombinant DNA by action of a restriction
endonuclease. Which one of the given option
incorrectly identifies the steps, components
labelled as A, B, C, and D?
1. Sparged stirred tank type
2. Airlift type
3. Simple stirred tank type
4. Fluidized bed type
25. Assertion: Agrobacterium tumefaciens
is popular in genetic engineering because
this bacterium is associated with roots of
all cereals and pulse crops
Reason : A gene incorporated in the
bacterial chromosomal genome gets
automatically transferred to the crop with
which the bacterium is associated
[a] Both Assertion and Reason are true and
Reason is correct explanation of Assertion.
[b] Both Assertion and Reason are true, but

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 Reason is not the correct explanation of
Assertion.
[c] Assertion is true, but Reason is false.
[d] Assertion is false, but Reason is true.

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 Answer key
Ques 1 2 3 4 5 6 7 8 9
Ans 1 1 2 2 3 2 3 2 1
Ques 10 11 12 13 14 15 16 17 18
Ans 2 4 2 1 4 3 3 1 4
Ques 19 20 21 22 23 24 25
Ans 2 1 3 4 1 3 4

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