Life Sciences / Practical Task Grade 11 Page 1 Of 6 Limpopo DoE / February 2023

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NATIONAL
SENIOR CERTIFICATE

GRADE 11

LIFE SCIENCES PRACTICAL TASK 1

16 FEBRUARY 2023

MARKS: 40
TIME: 1 ½ hour
This question paper consists of 06 pages.

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PRACTICAL TASK: THE PREVALENCE OF BREAD MOULD (Rhizopus sp.)

NAME OF THE SCHOOL: _________________________________

NAME OF THE LEARNER: ________________________________

DATE: ______________

Mould is something that we often take for granted, as something that makes us have to
throw the bread away or the cheese smell bad. Mould is, in fact, a fascinating organism
which has had many different uses over the years and our lives would not be the same
without it. Most of us know that food seems to become mouldy more quickly in certain
substances than in the other.

MATERIALS/ APPARATUS

1. Slices of white bread

2. A plastic sandwich bag (sealable)

3. Water, in a small container

4. Surgical gloves (recommended but optional)

5. Lemon juice

6. Sugar water(Sugar solution)

7. Hand lens

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INSTRUCTIONS AND METHOD

1. Arrange your leaners into a manageable group.

2. Take four slices of white bread for each group.
3. Put different substances on each slice of bread as follows:
3.1. Slice 1: dry bread
3.2. Slice 2: 20 ml of tap water
3.3. Slice 3: 20 ml of lemon juice
3.4. Slice 4: 20ml sugar water
4. Slices of bread – Any sort will do but it is perfectly fine to use cheap white sliced
bread as then you will know that all of the slices are of a similar size, weight and
thickness.
5. Put each slice of bread was in a plastic sandwich bag (sealable)
6. Plastic sandwich bags must be marked accordingly.
7. Keep in a dark cupboard at a temperature of about 25oC for one week.
8. Remove all slices for observations.
9. RESULTS: Observe the growth of mould on the four slices of bread and work out
the percentage area of the bread covered by mould. You can express your
results in percentage, for example if half area of the slice is covered by the
mould then it is 50%.
10. Once you have finished, safely dispose of all the bags without opening them.

PRECAUTIONS: Please note that some people are allergic to mould; ask your
doctor or parents. Always wear gloves and a mask, wash your hands, and don’t
eat or drink whilst you are performing this study

******* What I see, hear, smell, touch and taste in the laboratory, I won’t forget*******

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Life Sciences / Practical Task Grade 11 Page 4 Of 6 Limpopo DoE / February 2023
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ACTIVITY 1

1.1. TEACHER OBSERVATION DURING EXPERIMENT (15)

ASSESSMENT 0 MARK 1 MARKs 2 MARKS 3 MARKS COMMENT

CRITERIA S
1.1.1. Follow Do not Follow Follows all Follows all
instruction follow some precaution precautionary
s. precautionar precautionar ary measures and
y measures y measures measures cautions other
group members
1.1.2. Handle Handle Handle Handle Handle
equipment/ materials materials materials materials
materials with with minimal without confidently and
maximum assistance assistance assist other
assistance group members
1.1.3. Make Unable to Able to Able to Able to observe
observatio observe observe observe slice coverage
ns slice slice slice percentage in
coverage coverage coverage three slices
percentage percentage percentage
in all slices in one slice. in two
slices
1.1.4. Record Table was Table was Table was Table was fully
information not partially fully completed with
/ data completed completed completed correct data
with some
incorrect
data
1.1.5. Measurem None of the Two Three All
ent measureme substances substance measurements
nts was measured s done correctly
done correctly measured
correctly correctly
TOTAL:
[15]

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1.2. RESULTS
1.2.1. Table: Complete the percentage mould coverage for each bread slice. (4)

Slices 1 2 3 4
Percentage(%)

1.2.2. Plot this information in the bar graphs on the same system of axes. (6)

1.2.3. Where does mould come from? (How did it get into the bread?)
________________________________________________________________ (1) .

1.2.4. State a hypothesis for this investigation.

________________________________________________________________ (1)

1.2.5. Name the dependent variable.

________________________________________________________________ (1)

1.2.6. Explain why mould did not grow well on the bread sprinkled with lemon juice?

________________________________________________________________ (2)

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1.2.7. Why was dry bread included in the experiment?

________________________________________________________________ (2)
1.2.8. How can you ensure reliability in this investigation?
________________________________________________________________ (1)
1.2.9. State TWO ways in which the validity of this investigation was ensured.
________________________________________________________________
________________________________________________________________ (2)
1.2.10. Suggest ONE way on how fungus can be used in the medical industry.
________________________________________________________________
________________________________________________________________ (2)
1.2.11. Questions are based on the following micrograph:

Identify the parts labelled A, B, C,

A __________________________
B___________________________
C___________________________ (3)
[25]
GRAND TOTAL: 40

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