Lab Number:

Date:

Title: An investigation showing that carbon dioxide is produced when yeast respires anaerobically.

Aim: To show that carbon dioxide is produced during anaerobic respiration in yeasts.

Apparatus and Materials: 4 boiling tubes, Bunsen burner, tripod stand, wire gauze, 3 500ml beakers,
delivery tube, rubber bungs, test tube rack, glass rod, pipette, tap Place water, limewater, liquid paraffin,
yeast and brown sugar solution.

Diagram of Apparatus:

Method:

1. Place 10 mL of the yeast-brown sugar solution into 2 separate boiling tubes, labelled A and B.
2. Place tube B into a boiling water bath for 15 minutes.
3. Remove tube B and add a 1cm-thick layer of liquid paraffin gently down the sides of both tubes
using a pipette.
4. Stopper both tubes using the rubber bungs with the attached delivery tubes.
5. Place both experimental tubes into a water bath maintained at 38°C.
6. Submerge the open ends of each delivery tube into separate boiling tubes which have been
half-filled with limewater.
7. Record all observations made.

Results: Record your observations in an appropriately designed table.

Discussion:

1. Define the terms aerobic and anaerobic respiration.

2. Explain the purpose of the liquid paraffin.
3. Why was the gas produced bubbled through limewater?
4. What was the purpose of boiling tube B?
5. Why were both tubes placed into a water bath that was maintained at 38°C?
6. Explain the results observed for each tube.

Precautions: State TWO precautions taken either for safety or for accuracy.

Sources of Error: State TWO sources of error which were within your control and may have resulted in
inaccurate results.

Limitations: State any TWO factors over which you had no control and which may have affected the
results.

Conclusion: Give a concise statement which answers the aim.

Reflection: See syllabus document for guidelines.