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Total No. of Printed Pages: 03

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SUBJECT CODE NO: - FF-6502

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FACULTY OF SCIENCE AND TECHNOLOGY

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M.Sc. (Sem-I) (Chemistry)

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Examination January-2023

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Analytical Chemistry -CHE - 101

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[Time: 3:00 Hours] [Max. Marks: 80]

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Please check whether you have got the right question paper.

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N. B 1) Question1 is compulsory.
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2) Solve any five questions from question number 2 to 8.
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Q1 Attempt the following. 20
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a. Explain in brief rate theory of chromatography.

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b. Describe the methods for development of chromatogram.

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8

7
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c. Discuss in brief the types of error.

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d. Write a short note on ‘The F test’
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e. A batch of nuclear fuel pellets was weighted to determine if they fell within
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control guidelines. The weights were 127.2; 128.4; 127.1; 129.0 and 128.1gm.
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Calculate the mean and range of the set of observations.

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f. Discuss the factors affecting on the column efficiency.

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g. Explain the terms normal phase chromatography and reverse phase

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chromatography.
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h. Enlist the types of detectors used in HPLC.

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i. Describe the principle of liquid-liquid partition chromatography.

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j. Give the applications of gas chromatography.

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Q2 Attempt the following. 12

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a. The precision of a method is being established, and the following data are
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obtained.
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22.23; 22.18; 22.25; 22.09 and 22.17%

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Is 22.09 a valid measurement at the 95% confidence level?

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[ Qtable: 0.710]
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03C55FB0889C3EA8A1E7B42729632F6E
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 72
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32
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b. Write notes on –

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i. Significant figures

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ii. The confidence limit

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Q3 Attempt the following. 12

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a. Arsenic (III) is 70% extracted from 7m HCL into an equal volume of toluene.

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What percentage will remain unextracted after three individual extractions with

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toluene?
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b. What is distribution ratio and distribution coefficient? Derive the equation

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showing relationship between distribution ratio and distribution coefficient.
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Q4 Attempt the following. 12

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a. What is zone broadening? Discuss the different factors affecting on it.

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b. Write notes on
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i. Selection of chromatograph system.

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ii. Plate theory of chromatography
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Q5 Attempt the following. 12

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72
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a. What is the principle of size exclusion chromatography? Write a note on gel

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A
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63

preparation and column packing in gel permeation chromatography.

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b. How do the stationary and mobile phases selected in the partition

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chromatography ?
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Q6 Attempt the following. 12

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a. Explain with labelled diagram instrumentation of gas chromatography.

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b. What is the principle of HPLC?

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3C

Explain in detail applications of it.

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03

03C55FB0889C3EA8A1E7B42729632F6E
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C5 9C E7 96 3C
5F 3E B4 32 55
B0 A8 2 F6 FB
03 88 A1 72 E0
3C 088
C5 9C E7 96 9C
5F 3E B4 32 55 3E
B0 A8 2 F6 FB
88 A1 72 E0
3C 0 88
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08 A8 27 F6 FB A8 B4
89 A1 29 E0 08 27
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number
2F 5F C3 E7 63 C5
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8
B4
27 6E B0 E A8 B4 2F 5FB
A1 29 03 88 27 6E 08
C3 E7 63 C5 9C A1 29 03 89

b. Write notes on
63

Q8 Attempt the following.

Q7 Attempt the following.

2F 5F E7 C5
EA B4 3E C3 2F 5

chromatography.
8 2 72 6E B0 A8
27
B4 EA 6E FB

0.798 ×523
A1 96 03
C 88 A 2 0 8A 08

0.0164 ×127.45
E7
B4 3 2 5 5F
9C
3E
1E
7B
96
32
3C
55
89
C3 1E
27 F6 B A 4 F F E 7B
29 E0 0 8 8 2 7 6E B 0 A 42
63 3C 89 A1 29 03 8 8 8A 72
55 C3 E7 63 C5 9C 1E 96
0.00675; 390.07; 100.0; 0.089

2F FB EA B4 2F 5F 3 7 32
6E 2 6 B EA B4
03 08 8 72 E 0 0 8 8 2 7
F6
C5 89 A1 96 3C 89 A 29 E0
ii) Give the answer of the following.
indicate which zeros are significant?

5F C3 E7 3 5 C 1E 6 3C

3
B0 EA B4 2F 5F 3 E 7B 32
F
55
88 8A 27 6E B0 A 8 4 6 E
FB
9C 1E 29 03 8 89 A 27
29 0 08
63 C5 C 1E 6 3C 89
3E 7B 2 5F 3E 7B 32 55

ii) Quantitative utility of gas chromatography.

A8 42 F6 C3
72 E0 B0 A8 42 F6 FB EA
A1 9 3 8 8 A 7 2 E0 0 8 8A

i) HPLC adsorption and partition chromatography

E7 63 C5 9C 1E 96 3C 89
B4 2F 5F 3 EA 7 B4 3 2F 5 5F C 3E
27 6E B0 2 6

03C55FB0889C3EA8A1E7B42729632F6E
29 0 3 8 8 A 7 E 03
B0
8
A8
63 C5 89
C3 1 E7
29
63 C5 8 9C A1
2F 5F EA B4 2F 5F 3E E7
6E B0 2 6 B
03 88 8A 72 E0 A
a. Compare the gel permeation chromatography and ion-exchange

3C 08 8A
C5 9C 1E 96 89
5F 3E 7 B 3 2 55 C 3
1E
B0 A8 42 F 6E FB EA 7B
88 A 72 03 0 8 8A 42
9C 1E 96 C5 8 9C 1E
72
3E 7B 32 5F 3E 7B
F6
b. Explain in detail theory of phase contact method for repeated equilibrations.

42 B A
a. i) List the proper number of significant figures in the following numbers and

A8 E
7 42
Operation to the maximum number of significant figures and indicate the key

A1 29 03 08 8A 72
E7 63 89 1E
2
C5 C 3
96
B4
27 F 6E
5F E A
7B
4
32
12
12

B0 2
29 03 88 8A 72
FF-6502

63 C5 9C 1E 96
2F 5F 3 7 B 32
6E B0 EA
8 4 F6
E
03 8 A 27
C5 89
C3 1 E7
29
63
5F EA B4 2F
B0
8 8 27 6E