Printed on 2/9/2024 8:15:03 PM

LECTURE PLAN

Institute/Department UNIVERSITY INSTITUTE Program Bachelor of Science

OF BIO-TECHNOLOGY (Biotechnology)(BS201)
(UIBT)
Master Subject Coordinator Nitu Rani Master Subject Coordinator E- E6742
Name: Code:
Course Name Cell and Tissue Culture Course Code 21BTT-357
Technology

Lecture Tutorial Practical Self Study Credit Subject Type

3 0 0 0 3.0 T

Course Type Course Category Mode of Assessment Mode of Delivery

N.A Graded (GR) Theory Examination (ET) Theory (TH)

Mission of the 1. Providing state-of-the-art infrastructure which will help excel in the field of research, innovation and entrepreneurship.
Department 2. Formulating a curriculum that is compatible with major national and international institutes to prepare the students to meet the
global needs of industry and society.
3. To enrich students and research scholars with domain-specific knowledge to function as competent professional
biotechnologists.
4. To promote research through academic and industrial collaborations at the national and international levels.
5. To provide an environment for preparing students for a robust and sustainable society, by participation in community service
and environmental protection.
Vision of the To be recognized as an institute, committed to excel in the field of research and innovation in Biotechnology and allied fields
Department through experiential learning to produce professional leaders.

Program Educational Objectives(PEOs)

PEO1 CU Biotechnology graduates will be well prepared to be successful in their profession as renowned academicians with their
theoretical and practical knowledge either in industries or academia in one or more of discipline and / or sub disciplines of
Biotechnology Research, Innovation, Consultancy and Entrepreneurship relevant to the society
PEO2 CU Biotechnology graduates will be academically prepared to provide feasible and sustainable solutions for real life problems; and
become expertise professionals in due course and will contribute and research skills and nurture entrepreneurial endeavours.
PEO3 CU Biotechnology graduates will be prepared to be engaged in professional activities to enhance their own stature with
professional grooming and simultaneously contribute for nation building and to solve global challenges in service of humankind.
PEO4 CU Biotechnology graduates will be prepared to be successful in their higher education in science and technology, with strategic
learning in leading National and International corporates and universities for academic as well as research, if pursued.

Program Specific OutComes(PSOs)

PSO1 CU Biotechnology graduates will be prepared to become efficient skilled resources to execute their knowledge effectively in areas
of Biotechnology and allied disciplines and, to perform their duties efficiently in both Govt and nongovernment organizations
PSO2 CU Biotechnology graduates will be empowered with scientific analysis and thinking, Communication skills, Bioethics, IPR,
entrepreneurship, and professional ethics to provide enabling their applications in industry and research in next generation
Biotechnology industries.
PSO3 CU Biotechnology graduates will be able to provide scientifically feasible, economically viable and environmentally sustainable
solutions to regional, national and global real-life problems for benefit of humankind.

Program OutComes(POs)
PO1 Disciplinary Knowledge: Grasp basic knowledge on microorganisms and their role in ecosystem and demonstrate practical skills to
work with microorganisms.
PO2 Communication Skills: Communicate efficiently with scientific community and society

PO3 Coordinating/collaborating with others: Function efficiently both as an individual, and as a member or leader in various teams, and
in multidisciplinary scientific activities.
PO4 Learning how to learn skills: Recognize the need and can engage in independent and life-long learning in the broadest context of
technological change

University Information System - By - ERP Division Page 1 of 7

 Printed on 2/9/2024 8:15:03 PM
LECTURE PLAN

PO5 Digital and technological skills: Demonstrate competencies in the use of digital technology needed to access learning
opportunities, to pursue chosen careers and to contribute to society as active citizens
PO6 Multicultural competence and inclusive spirit: Demonstrate multidimensional worldview and foster the capacity to imagine, create
and collaborate across and within multicultural, transcultural and intercultural spaces.
PO7 Autonomy, responsibility, and accountability: Demonstrate decision making ability, along with professional and ethical
accountability in the scope of a goal.
PO8 Environmental awareness and action: Understand the influence of the professional scientific solutions in societal and
environmental contexts, and determine the biological knowledge and its need for sustainable development
PO9 Community engagement and service: Reflect ability to link personal and social development to achieve real objectives for the
community through community engagement and service.
PO10 Critical thinking: Identify, formulate, analyze, and investigate complex scientific problems reaching validated conclusions using
both theoretical and practical basic principles of Life sciences
PO11 Creativity: Design solutions for complex biological problems and design innovative components or processes that meet the
specified needs with suitable contemplation for the public health, safety, cultural, societal, and environmental considerations.
PO12 Analytical reasoning/thinking: Use, apply and generate suitable bio-techniques, resources, and modern interdisciplinary tools to
complex biological activities with an understanding of the boundaries

Text Books
Sr No Title of the Book Author Name Volume/Edition Publish Hours Years
1 Plant biotechnology: the genetic Slater 2nd Oxford 2008
manipulation of plants
2 An introduction to Plant Tissue Razdan MK. 2nd Oxford & IBH 2013
culture Publishing Co,
New Delhi.
3 Animal Cell Culture and Michael Butler 2nd Garland 2003
Technology Science/BIOS
Scientific
Publishers.

Reference Books
Sr No Title of the Book Author Name Volume/Edition Publish Hours Years
1 Handbook of Plant Biotechnology Christou P & Klee H. 3rd John Wiley & 2011
Sons
2 Plant Propagation by Tissue George EF, Hall MA & De Klerk 4th Agritech Publ. 2018
Culture GJ.

Course OutCome
SrNo OutCome
CO1 Understanding various components of cell and tissue culture media, e.g. minerals, growth factors,
hormones, and what governs the choice of components
CO2 Successfully perform a variety of cell and tissue culture techniques.
CO3 Apply cell and tissue culture techniques to address specific research questions or biotechnological
challenges.
CO4 Student able to make the wider employment opportunities with knowledge of plant and animal culture
technology

Lecture Plan Preview-Theory

Mapped with
Pedagogical
Unit No LectureNo ChapterName Topic Text/ Reference Books CO Numer
Tool**
(s)
1 1 Introduction to History and landmark discoveries ,T-An introduction to Plant Tissu,T- PPT CO1
plant tissue Plant biotechnology: the genet,R-
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu

University Information System - By - ERP Division Page 2 of 7

 Printed on 2/9/2024 8:15:03 PM
LECTURE PLAN

1 2 Introduction to Terminology in plant tissue culture. ,T-An introduction to Plant Tissu,T- Activity,Case CO1
plant tissue Plant biotechnology: the genet,R- Study,Flippe
culture Handbook of Plant Biotechnolog,R- d
Plant Propagation by Tissue Cu Classes,Info
graphics,Inst
ructor Lead
WorkShop,P
PT,Professo
r of
Practice/Adj
unct
Faculty/Visiti
ng
Professor,R
eports,Video
Lecture
1 3 Introduction to Plant tissue culture media. ,T-An introduction to Plant Tissu,T- PPT,Video CO1
plant tissue Plant biotechnology: the genet,R- Lecture
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 4 Introduction to Plant growth regulators – Part 1 ,T-An introduction to Plant Tissu,T- PPT CO1
plant tissue Plant biotechnology: the genet,R-
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 5 Introduction to Plant growth regulators – Part 2 ,T-An introduction to Plant Tissu,T- PPT CO1
plant tissue Plant biotechnology: the genet,R-
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 6 Sterilization Sterilization and its type – Part 1 ,T-An introduction to Plant Tissu,T- PPT CO2
techniques Plant biotechnology: the genet,R-
Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 7 Sterilization Sterilization and its type – Part 2 ,T-An introduction to Plant Tissu,T- PPT CO2
techniques Animal Cell Culture and Techno,T-
Plant biotechnology: the genet,R-
Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 8 Sterilization Storage of Media ,T-An introduction to Plant Tissu,T- PPT,Video CO2
techniques Plant biotechnology: the genet,R- Lecture
Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 9 Sterilization Murashige and Skoog media ,T-An introduction to Plant Tissu,T- PPT,Video CO2
techniques composition – Part 1 Plant biotechnology: the genet,R- Lecture
Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 10 Sterilization Murashige and Skoog preparation ,T-An introduction to Plant Tissu,T- PPT,Video CO2
techniques (mapped with CO1, CO2) Plant biotechnology: the genet,R- Lecture
Plant Propagation by Tissue Cu
1 11 Techniques in Isolation of explant, types of plant ,T-An introduction to Plant Tissu,T- PPT,Video CO2
plant tissue tissue techniques Plant biotechnology: the genet,R- Lecture
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 12 Techniques in Sterilization of explant ,T-An introduction to Plant Tissu,T- PPT,Video CO2
plant tissue Plant biotechnology: the genet,R- Lecture
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 13 Techniques in Isolation of single cell ,T-An introduction to Plant Tissu,T- PPT,Video CO2
plant tissue Plant biotechnology: the genet,R- Lecture
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
1 14 Techniques in Cryoperservation – Part 1 ,T-An introduction to Plant Tissu,T- PPT,Video CO2
plant tissue Plant biotechnology: the genet,R- Lecture
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu

University Information System - By - ERP Division Page 3 of 7

 Printed on 2/9/2024 8:15:03 PM
LECTURE PLAN

1 15 Techniques in Cryoperservation – Part 2 ,T-An introduction to Plant Tissu,T- PPT,Video CO2

plant tissue Plant biotechnology: the genet,R- Lecture
culture Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
2 16 Plant tissue Types of plant tissue culture ,T-An introduction to Plant Tissu,T- PPT,Video CO2
culture and its Plant biotechnology: the genet,R- Lecture
types Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
2 17 Plant tissue Callus culture ,T-An introduction to Plant Tissu,T- PPT,Video CO2
culture and its Plant biotechnology: the genet,R- Lecture
types Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
2 18 Plant tissue Organ Culture ,T-An introduction to Plant Tissu,T- PPT,Video CO2
culture and its Plant biotechnology: the genet,R- Lecture
types Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
2 19 Plant tissue Anther culture ,T-An introduction to Plant Tissu,T- PPT,Video CO2
culture and its Plant biotechnology: the genet,R- Lecture
types Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
2 20 Plant tissue Protoplast culture ,T-An introduction to Plant Tissu,T- PPT,Video CO2
culture and its Plant biotechnology: the genet,R- Lecture
types Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
2 21 Steps involved Various steps in micropropogation -Part ,T-An introduction to Plant Tissu,T- PPT,Video CO2
in 1 Plant biotechnology: the genet,R- Lecture
micropropogati Handbook of Plant Biotechnolog,R-
on Plant Propagation by Tissue Cu
2 22 Steps involved Various steps in micropropogation -Part ,T-An introduction to Plant Tissu,T- PPT,Video CO2
in 2 Plant biotechnology: the genet,R- Lecture
micropropogati Handbook of Plant Biotechnolog,R-
on Plant Propagation by Tissue Cu
2 23 Steps involved Factors affecting micropropogation ,T-An introduction to Plant Tissu,T- PPT,Video CO2
in Plant biotechnology: the genet,R- Lecture
micropropogati Handbook of Plant Biotechnolog,R-
on Plant Propagation by Tissue Cu
2 24 Steps involved Applications of micropropagation ,T-An introduction to Plant Tissu,T- PPT,Video CO3
in Plant biotechnology: the genet,R- Lecture
micropropogati Handbook of Plant Biotechnolog,R-
on Plant Propagation by Tissue Cu
2 25 Steps involved Virus free plant production ,T-An introduction to Plant Tissu,T- Activity,Case CO3
in Plant biotechnology: the genet,R- Study,Flippe
micropropogati Handbook of Plant Biotechnolog,R- d
on Plant Propagation by Tissue Cu Classes,Info
graphics,Inst
ructor Lead
WorkShop,P
PT,Professo
r of
Practice/Adj
unct
Faculty/Visiti
ng
Professor,R
eports,Simul
ation,Video
Lecture
2 26 Transgeneic Transgenics and its importance in crop ,T-An introduction to Plant Tissu,T- PPT,Video CO3
and GMO’s improvement Plant biotechnology: the genet,R- Lecture
Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu

University Information System - By - ERP Division Page 4 of 7

 Printed on 2/9/2024 8:15:03 PM
LECTURE PLAN

2 27 Transgeneic Applications of Transgenics in crop ,T-An introduction to Plant Tissu,T- PPT,Profess CO3
and GMO’s improvement Plant biotechnology: the genet,R- or of
Handbook of Plant Biotechnolog,R- Practice/Adj
Plant Propagation by Tissue Cu unct
Faculty/Visiti
ng
Professor,Vi
deo Lecture
2 28 Transgeneic Genetically modified crops and its ,T-An introduction to Plant Tissu,T- PPT,Video CO3
and GMO’s important Plant biotechnology: the genet,R- Lecture
Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
2 29 Transgeneic Organogenesis and embryogenesis ,T-Plant biotechnology: the genet,R- PPT,Video CO3
and GMO’s Handbook of Plant Biotechnolog,R- Lecture
Plant Propagation by Tissue Cu
2 30 Transgeneic Synthetic seeds and their significance ,T-An introduction to Plant Tissu,T- PPT,Video CO3
and GMO’s Plant biotechnology: the genet,R- Lecture
Handbook of Plant Biotechnolog,R-
Plant Propagation by Tissue Cu
3 31 Introduction to Terminology in Animal tissue culture ,T-Animal Cell Culture and PPT,Video CO3
animal culture Techno,R-Handbook of Plant Lecture
Biotechnolog
3 32 Introduction to Historical aspects of Animal tissue ,T-Animal Cell Culture and PPT,Video CO3
animal culture culture Techno,R-Handbook of Plant Lecture
Biotechnolog
3 33 Introduction to Types of culture media in Animal tissue ,T-Animal Cell Culture and PPT,Video CO3
animal culture culture – part 1 Techno,R-Handbook of Plant Lecture
Biotechnolog
3 34 Introduction to Types of culture media in Animal tissue T-Animal Cell Culture and Techno PPT,Video CO3
animal culture culture – part 2 Lecture
3 35 Introduction to Components of culture media ,T-Animal Cell Culture and PPT,Video CO3
animal culture Techno,R-Handbook of Plant Lecture
Biotechnolog
3 36 Introduction to Components of culture media- part 2 ,T-Animal Cell Culture and PPT,Video CO3
animal culture Techno,R-Handbook of Plant Lecture
Biotechnolog
3 37 Growth, Growth of animal cell in Animal culture - ,T-Animal Cell Culture and PPT,Video CO4
characteristics part 1 Techno,R-Plant Propagation by Lecture
of Animal Tissue Cu
Culture
3 38 Growth, Growth of animal cell in Animal culture - T-Animal Cell Culture and Techno PPT,Video CO4
characteristics part 2 Lecture
of Animal
Culture
3 39 Growth, Maintenance of animal cell in Animal ,T-Animal Cell Culture and PPT,Video CO4
characteristics culture -part 1 Techno,R-Handbook of Plant Lecture
of Animal Biotechnolog
Culture
3 40 Growth, Maintenance of animal cell in Animal ,T-Animal Cell Culture and PPT,Video CO4
characteristics culture -part2 Techno,R-Handbook of Plant Lecture
of Animal Biotechnolog
Culture
3 41 Growth, Characteristics of different animal cell in ,T-Animal Cell Culture and PPT,Video CO4
characteristics Animal culture Techno,R-Handbook of Plant Lecture
of Animal Biotechnolog
Culture
3 42 Growth, Characteristics of different animal cell in ,T-Animal Cell Culture and PPT,Video CO4
characteristics Animal culture - part 2 Techno,R-Handbook of Plant Lecture
of Animal Biotechnolog
Culture
3 43 Growth, Introduction to stem cells and skin ,T-Animal Cell Culture and PPT,Video CO4
characteristics grafting – part 1 Techno,R-Handbook of Plant Lecture
of Animal Biotechnolog
Culture

University Information System - By - ERP Division Page 5 of 7

 Printed on 2/9/2024 8:15:03 PM
LECTURE PLAN

3 44 Growth, Introduction to stem cells and skin ,T-Animal Cell Culture and PPT,Video CO4
characteristics grafting – part 1 Techno,R-Handbook of Plant Lecture
of Animal Biotechnolog
Culture
3 45 Growth, Introduction to stem cells and skin T-Animal Cell Culture and Techno PPT,Video CO4
characteristics grafting – part 2 Lecture
of Animal
Culture

Assessment Model
Sr No Assessment Name Exam Name Max Marks
1 20SU06 External Theory 60
2 20SU06 Assignment 10
3 20SU06 Attendance Marks 2
4 20SU06 Mid-Semester Test-1 20
5 20SU06 Quiz 4
6 20SU06 Surprise Test 12
7 20SU06 Mid-Semester Test-2 20

CO vs PO/PSO PSO3 PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12 PSO1 PSO2

CO1 NA 2 1 2 1 NA NA 1 NA 3 NA 1 1 1 1

CO2 1 NA 1 NA 2 NA 1 NA 1 NA 2 1 1 2 3

CO3 NA 1 NA 2 NA 1 3 3 NA NA 2 NA 3 2 NA

CO4 NA 2 2 NA 3 3 NA 3 3 2 NA NA NA NA 3

Target 1 1.67 1.33 2 2 2 2 2.33 2 2.5 2 1 1.67 1.67 2.33

University Information System - By - ERP Division Page 6 of 7

 Printed on 2/9/2024 8:15:03 PM
LECTURE PLAN

University Information System - By - ERP Division Page 7 of 7