INSTRUMENTAL BY-

METHOD OF BHUPESH,RAGHAV,
ABHAY,RISHAB
ANALYSIS: CASE B.PHARM 7th
STUDY Semester
CASE STUDY THAT COMPARES THE USE
OF GAS CHROMATOGRAPHY (GC) AND
HIGH-PERFORMANCE LIQUID
CHROMATOGRAPHY (HPLC) FOR THE
ANALYSIS OF A SPECIFIC COMPOUND IN
ENVIRONMENTAL SAMPLES
COMPARATIVE ANALYSIS OF GC AND HPLC FOR THE DETECTION
OF BENZENE IN ENVIRONMENTAL WATER SAMPLES

▪ Introduction: Benzene is a hazardous volatile organic compound that

can contaminate water sources. This case study aims to compare Gas
Chromatography (GC) and High-Performance Liquid Chromatography
(HPLC) for the quantification of benzene in environmental water
samples, with a focus on their respective strengths and limitations.

▪ Objective: To determine which chromatographic technique, GC or

HPLC, is better suited for the sensitive and accurate quantification of
benzene in water samples.
 METHODS

▪ Sample Collection: Water samples from a contaminated site

were collected and prepared for analysis.

▪ Sample Preparation: Samples were processed to extract

benzene, including appropriate extraction and concentration
steps.
 INSTRUMENTATION
▪ Gas Chromatography (GC):A GC system equipped with a capillary column and a
Flame Ionization Detector (FID) was employed.
GC offered excellent separation for volatile compounds like benzene and high
sensitivity.

▪ High-Performance Liquid Chromatography (HPLC): An HPLC system with a

reverse-phase C18 column and UV-Vis detection was used.
HPLC provided good solubility for benzene and the ability to separate compounds with
similar polarities.
 RESULTS
▪ Gas Chromatography: GC analysis demonstrated superior
sensitivity and resolution, enabling the precise quantification
of benzene at low concentrations in the water samples.

▪ HPLC: HPLC showed good sensitivity as well but was

somewhat less selective for benzene compared to GC due to
the matrix effect in water samples.
 CONCLUSION
▪ Both GC and HPLC can be used effectively for the analysis of benzene in
water samples, with GC providing better sensitivity and separation. However,
HPLC may require additional sample preparation steps to remove matrix
interferences.

▪ The choice between GC and HPLC depends on the specific analytical goals
and the nature of the sample matrix. In the case of benzene analysis in water
samples, GC may be preferred for its higher sensitivity and selectivity.
However, HPLC remains a valuable alternative, especially if extensive sample
preparation is needed.
THIS CASE STUDY ILLUSTRATES THE
IMPORTANCE OF SELECTING THE MOST
APPROPRIATE CHROMATOGRAPHIC TECHNIQUE
BASED ON THE SPECIFIC ANALYTICAL
REQUIREMENTS AND THE PROPERTIES OF THE
COMPOUNDS AND MATRICES BEING ANALYZED.
THANK
YOU