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Lesson 10
Lesson 10
OUTLINE o Urine
I. Overview o Amniotic fluid
II. Non-blood Specimen Labeling and Handling o Cerebrospinal fluid
III. Non-blood Body-Fluid Specimens (11)
o Gastric fluid / Gastric Analysis
A. Urine
o Nasopharyngeal Secretions
B. Amniotic Fluid
o Saliva
C. CSF
o Semen
D. Gastric Fluid / Gastric Analysis
E. Nasopharyngeal Secretions, etc o Serous fluid
IV. Other Non-blood Specimens o Sputum
o Sweat
o Synovial fluid.
I. OVERVIEW ● Its function is to serve as a lubricant so that there could be
● Although blood is the specimen of choice for many laboratory distance between the tissues and surrounding tissues.
tests, various other body substances are also analyzed. ● It also serves to lubricate the various organs.
● The phlebotomist may be involved in obtaining the specimens
(e.g., throat swab collection), test administration (e.g., sweat URINE
chloride collection), instruction (e.g., urine collection), ● Is the most frequently analyzed non-blood body fluid.
processing (accessioning and preparing the specimen for ● It is the focus specimen for clinical microscopy. It is a
testing), or merely labeling or transporting the specimens to the non-invasive collection procedure since you just ask the patient
lab. to void his or her urine.
● This chapter addresses routine and special nonblood ● Urine has been studied since the very beginning of laboratory
specimens and procedures, including the collection and medicine.
handling of urine specimens and other non blood body fluids ● It is readily available, easy to collect, and generally inexpensive
and substances. to test, its analysis can provide information on many of the
● A phlebotomist with a thorough understanding of all aspects of body's major metabolic functions.
nonblood specimen collection helps ensure the quality of the ● Normally, urine is the color yellow. If the urine is mostly
specimens and the accuracy of test results. concentrated, it is described to be dark yellow in color.
● Analysis of urine can aid in.
II. NON-BLOOD SPECIMEN LABELING AND o monitoring wellness,
HANDLING o the diagnosis and treatment of urinary tract infections,
● Proper labeling helps avoid testing delays, which can o the detection and monitoring of metabolic disease,
compromise patient care. Nonblood specimens should be o and determining the effectiveness or complications of
labeled with the same identifying information as blood therapy
specimens. ● Accurate results depend on.
● In addition, since many body fluids are similar in appearance, o collection method
labeling should include the type and/or source of the specimen. o container used
● Because the lid is removed for testing, the label should be o specimen transportation and handling
applied to the container, not the lid, so as to avoid o timeliness of testing
misidentification. If urine is not tested in a timely fashion, this results in
● Nonblood specimens have various handling requirements. The erroneous results.
phlebotomist must be familiar with these requirements to ● If urine is not tested in a timely fashion, this results in erroneous
protect the integrity of the specimen and help ensure accurate results.
test results. ● Inpatient urine specimen collection is typically handled by
● In addition, all body substances are potentially infectious, and nursing personnel.
standard precautions must be observed in handling them. ● Outpatient urine specimen collection is often handled by
phlebotomists.
● The phlebotomist must be able to explain urine collection
III. NON-BLOOD BODY-FLUID SPECIMEN procedures without embarrassing the patient.
● Nonblood body fluids are liquid or semiliquid substances ● If urine specimens are not tested promptly, urine components
produced by the body and found in the intracellular and can change. For example, cellular elements decompose,
interstitial spaces and within various organs (e.g., the bladder) bilirubin breaks down to biliverdin, and bacteria multiply, leading
and body spaces (e.g., joints). to erroneous test results.
SEMEN
SWEAT
● Semen (seminal fluid) is the sperm-containing thick
● Sweat is analyzed for chloride content in the diagnosis of cystic
yellowish-white fluid discharge during male ejaculation.
fibrosis, predominantly in children and adolescents under the
● It is analyzed to assess fertility or determine the
age of 20.
effectiveness of sterilization following vasectomy.
● Cystic fibrosis is a disorder of the exocrine glands that
● It is also sometimes examined for forensic (or legal)
affects many body systems but primarily the lungs, upper
reasons (e.g., criminal sexual investigations).
respiratory tract, liver, and pancreas.
● Semen specimens are collected in sterile or chemically
● Patients with cystic fibrosis have abnormally high levels (two to
clean containers and must be kept warm, protected from light,
five times normal) of chloride in their sweat, which can be
and delivered to the lab immediately.
measured by the sweat chloride test.
● A semen specimen should not be collected in a condom unless
● The test involves transporting pilocarpine (a
it is one specifically designed for specimen collection.
sweat-stimulating drug) into the skin by means of electrical
Regular condoms often contain spermicides (substances that
stimulation from electrodes placed on the skin, a process called
kill sperm) that invalidate test results.
iontophoresis.
● The forearm is the preferred site, but the leg or thigh may be
SEROUS FLUID used on infants or toddlers.
● Serous fluid à is the pale-yellow, watery, serum-like fluid
found between the double-layered membranes enclosing the
SYNOVIAL FLUID
pleural, pericardial, and peritoneal cavities.
● Synovial fluid à is a clear, pale-yellow, viscous fluid that
● It lubricates the membranes and allows them to slide past one
lubricates and decreases friction in movable joints.
another with minimal friction.
● It normally occurs in small amounts but increases when
● The fluid is normally present in small amounts, but
inflammation is present.
volumes increase when inflammation or infection is present
● It can be tested to identify or differentiate arthritis, gout, and
or when serum protein levels decrease.
other inflammatory conditions.
● Effusion à An increase in fluid volume.
● It is typically collected in three tubes:
● Ascites à Accumulation of excess serous fluid in the
o an EDTA or heparin tube à for cell counts, identification of
peritoneal cavity, and the fluid is referred to as ascitic fluid.
crystals, and smear preparation;
● Serous fluids can be aspirated for testing purposes or when
o a sterile tube à for culture and sensitivity;
increased amounts are interfering with the normal function of
associated organs.
BREATH SAMPLES
● Breath samples are collected and analyzed for hydrogen
content in one type of lactose tolerance test and to detect the
presence of Helicobacter pylori (H. pylori)
● Helicobacter pylori à a type of bacteria that secretes
substances that damage the lining of the stomach and
causes chronic gastritis, which can lead to peptic ulcer disease.
FECES (STOOL)
● The fecal specimen (feces or stool) is collected
o (1) to determine gastrointestinal disorder,
o (2) to analyze for the presence of intestinal ova and
parasites (O&P),
o (3) to be cultured then examined for the presence of
pathogenic bacteria and viruses,
o (4) to check fat and urobilinogen content, or