µEncapsulator System Datasheet

Version: 5.0

Issue Date: 12 March 2020

Author: DR

Dolomite Microfluidics is a brand of Blacktrace

Registered office: The Dolomite Centre Ltd. 1 Anglian Business Park, Royston, SG8 5TW, UK
Reg in England No. 04257809
Contents
1 Disclaimer ................................................................................................................................... 3

2 Description and Part Numbers ........................................................................................... 4

2.1 µEncapsulator System ............................................................................................... 4

2.2 uEncapsulator Chips ................................................................................................... 5

3 Benefits ........................................................................................................................................ 5

4 System Specifications ............................................................................................................ 6

5 PC Specifications ..................................................................................................................... 7

6 Compressor Specifications................................................................................................... 7

7 Typical System Set-up ........................................................................................................... 8

8 Sample Reservoir Chip .......................................................................................................... 8

8.1 Features and benefits................................................................................................. 8

8.2 Geometry ......................................................................................................................... 9

9 µEncapsulator Droplet Chip................................................................................................. 9

9.1 Features and benefits.............................................................................................. 10

9.2 Geometry ...................................................................................................................... 11

10 P-Pump ..................................................................................................................................... 12

10.1 Features and benefits ........................................................................................... 12

10.2 Specifications ........................................................................................................... 12

11 Sensor Interphase/ Display ............................................................................................... 12

11.1 Features and benefits ........................................................................................... 13

12 Flow rate sensor.................................................................................................................... 13

12.1 Features and benefits ........................................................................................... 13

13 Microscope Specifications ................................................................................................. 14

14 Temperature Control Unit .................................................................................................. 15

14.1 Features and benefits ........................................................................................... 15

14.2 Specifications ........................................................................................................... 15

Dolomite Microfluidics Offices........................................................................................................ 17

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 2 of 17

1 Disclaimer
This product is for research use only and is not to be used for any other purposes,
including, but not limited to, use in drugs, in vitro diagnostics, or use in humans. This
document is confidential and is the property of property of Blacktrace Holdings Ltd.
Dolomite Microfluidics is a part of Blacktrace Holdings Ltd. No attempt should be
made to copy this document in any way without prior consent. © 2017 Blacktrace
Holdings Ltd. All rights reserved.

The document and its contents shall not be reproduced, disclosed, or used for any
other purpose without written consent of Dolomite Microfluidics. Dolomite
Microfluidics does not convey any license under its trademark, copyright, or common-
law rights nor similar rights of any third parties by this document.

Revisions and modifications of kit components and manuals will be performed as

appropriate. The instructions within this document must be followed rigorously by
qualified and trained personnel. Failure to adhere may result in damage to the
product, injury to persons or damage to other property. Dolomite Microfluidics shall
not be liable for any damages or expenses arising directly or indirectly from the use
of this product (including parts or software).

Dolomite Microfluidics makes no warranty of any kind, either expressed or implied.

This includes merchantability for this product, and the fitness of the product for any
purpose.

The µEncapsulator system is a trademark of Dolomite Microfluidics. All other brands

and names mentioned within this document are the property of their respective
owners.

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 3 of 17

2 Description and Part Numbers
• The cell encapsulation system enables the simple, quick and reliable
encapsulation of single cells, DNA and/or functionalized beads in high
precision, monodisperse picolitre droplets.

• To encapsulate a sample, the user loads sample and reagent into the
100 µl reservoirs, closes the module, and starts the program. The
droplets are collected in the built-in output reservoir. 200 µl of reagent
and sample, containing 300,000 cells, can be converted into 3 million
droplets in 15 minutes. The module can be maintained at 4°C with the
Dolomite Temperature Control Unit, the TCU-100. Droplet production
can be monitored with the Dolomite High Speed Digital Microscope.

• The hardware can be controlled by the µEncapsulator 1 program in the

Dolomite Flow Control Centre software. The necessary reagents for
encapsulation are included in the µEncapsulator 1 Reagent Kit

2.1 µEncapsulator System

Orders from Instrument/Consumables Order

Number
US and µEncapsulator System with Enhanced Control 3200554
Canada (110v, 60Hz, US) - Excludes Applications Pack
High Speed Digital Microscope and Camera 3200531
UK µEncapsulator System with Enhanced Control 3200556
(230V, 50Hz, UK) - Excludes Applications Pack
High Speed Digital Microscope and Camera 3200531
Europe µEncapsulator System with Enhanced Control 3200558
(230V, 50Hz, EU) - Excludes Applications Pack
High Speed Digital Microscope and Camera 3200531
Japan µEncapsulator System with Enhanced Control 3200560
(100V, 50-60Hz, JP) - Excludes Applications
Pack
High Speed Digital Microscope and Camera 3200531
Rest of the µEncapsulator System with Enhanced Control 3200556
world (230V, 50Hz, UK) - Excludes Applications Pack
High Speed Digital Microscope and Camera 3200531

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 4 of 17

 2.2 uEncapsulator Chips

Instrument/Consumables Order Number

µEncapsulator 1 Sample Reservoir Chip (2x 100 µl) Pack 3200562
of 3
µEncapsulator 1 - 2 Reagent Droplet Chip (50 µm etch 3200563
depth), fluorophilic, Pack of 3
µEncapsulator 1 - 2 Reagent Droplet Chip (50 µm etch 3200564
depth), hydrophilic, Pack of 3
µEncapsulator 1 - 2 Reagent Droplet Chip (30 µm etch 3200567
depth), fluorophilic, Pack of 3
µEncapsulator 1 - 2 Reagent Droplet Chip (30 µm etch 3200568
depth), hydrophilic, Pack of 3
µEncapsulator 1 - 2 Reagent Droplet Chip (15 µm etch 3200565
depth), hydrophilic, Pack of 3
µEncapsulator 1 - 2 Reagent Droplet Chip (15 µm etch 3200566
depth), fluorophilic, Pack of 3

3 Benefits
• High-throughput encapsulation of cells or nucleic acid in monodisperse
droplets

• Simple-to-use and reliable encapsulation

• Encapsulates 300,000 cells in 3 million droplets in 15 minutes

• Co-encapsulates 100 µl of sample and reagent, or two sequential 100 µl

samples, with minimal wastage

• Encapsulated samples are ready for downstream processes.

• Enables high throughput single cell expression profiling, or profiling natively

paired T cell receptors, isolating monoclonal antibody coding sequences
from blood samples, droplet thermocycling, encapsulation in hydrogels, etc.

• Inert, biocompatible carrier oil

• Biocompatible droplet reagents enabling cell culture studies

• Reagents for breaking emulsion on demand

• Temperature control to maintain cell viability

• P-Pump flow control for monodisperse droplets, with precisely controlled

and reproducible droplet volumes

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 5 of 17

4 System Specifications

Specification

Computer, microscope, 3 x P-
Pumps, uEncapsulator module, 3 x
Flow sensor interphase/ flow sensor
System components display, 3 x Flow sensors,
Temperature Control Unit,
Compressor, Sample reservoir chip,
Droplet Chip
Droplet Reagent input volume 2 x 100 µl
Droplet size range (1)
Ø 10 – 70 µm

Droplet volume range (1) 0.5 – 180 picoliters

3,000 droplets/second (for Ø 50 µm
Droplet frequency (1)
droplets)
2% FluoSurf, an oil commercialised
Carrier oil type
by Dolomite Microfluidics
Output emulsion reservoir volume 2 ml micro centrifuge tube
0.7 - 2 ml (for Ø 70 - 50 µm
Output emulsion volume (per run) (1)
droplets)
Output emulsion droplet count (per Approx. 3 million (for Ø 50 µm
run) (1) droplets)
Wetted materials FKM, Glass, FEP (tubing material)
Maximum pressure 5 bar
Temperature range 1 – 100 ºC
Maximum recommended size of
25 μm
encapsulated material (2)
Maximum recommended density of
1.3 g/ml
encapsulated material (2)
1300 mm (L) x 600 mm (W) x 600
Dimension (complete system)
mm (H)

(1) Specification dependent on exact reagents used, chip junction size and process
parameters selected

(2) Performance with encapsulated material outside of this range requires individual
testing

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 6 of 17

5 PC Specifications
Specification

PC: 0.649 kg
Weight Screen: 2.9 kg
Keyboard: 0.50 kg
Ampere 1A
Voltage 110 V
Operating Temperature 5 – 40 ˚C
Operating Humidity 20 – 80 %
PC:
120 mm (L) x 110 mm (W) x 50 mm (H)
Screen:
Dimension 498 mm (L) x 164 mm (W) x 387 mm
(H)
Keyboard:
190 mm (L) x 140 mm (W) x 25 mm (H)

6 Compressor Specifications
Specification

Weight 14 kg
Ampere 8.5 A
Voltage 120 V
Operating Temperature Up to 130 ˚C
This will depend upon CFM, PSI,
Operating Humidity Runtime (no values provided by
supplier)
360 mm (L) x 475 mm (W) x 343 mm
Dimension
(H)

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 7 of 17

7 Typical System Set-up

µEncapsulator
TCU Microscope

Flow sensor Microscope

power supply

P-pumps

Cells

Oil

Droplet chip Sample reservoir chip

2nd Aqueous line

8 Sample Reservoir Chip

The Sample Reservoir Chip (part no. 3200444) for the
µEncapsulator 1 module contains 2 reservoirs of 100 µl
volume for storage of biological samples at
temperature before droplet formation. Reagents are
pipetted in the inlet ports. There are 2 additional
straight through channels for carrier oil input and
emulsion output. The smooth channel surfaces and precision fabrication provide an
excellent flow path for passage of encapsulated material. The Sample Reservoir
Chip can be used in conjunction with the Droplet Chips (see Section 9) to form 2
reagent droplets.

8.1 Features and benefits

• 100 µl storage volume for 2 reagents
• Reagent 1 and 2 channel cross-section 1.00 mm x 1.40 mm (depth x
width)
• Reduced cross section for the oil-in and emulsion-out channels (0.50 mm
deep x 1.02 mm wide)
• Compatible with standard pipettes for injection of reagents
• Functionalised top surface to hold reagents in reservoirs
• Chip size 30 mm x 15 mm x 4.1 mm
• Fabricated in glass
• Very smooth channels (surface roughness Ra of 5 nm)
• Excellent optical clarity
• Easy to clean

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 8 of 17

 8.2 Geometry

9 µEncapsulator Droplet Chip

The Droplet Chips for the µEncapsulator 1 module enables 2-reagent droplets
containing biological material to be generated with ease. These chips interface
directly with the Sample Reservoir Chip (part no. 3200444) via the supplied FKM
gasket. It can also be addressed independently for basic tests using the Multiflux®
Linear Connector 4-way (part no. 3000024) and Top Interface 4-way 2.15 mm (part
no. 3000237).

The 2 reagent streams meet immediately before the junction, minimising mixing prior
to the formation of droplets. The Dolomite flow focussing junction design enables
thousands of reactions per second to be carried out in volume-controlled
compartments, isolated by a flowing carrier stream.

The uEncapsulator Chips are available with different coatings (hydrophilic and
fluorophilic) and junction sizes to cover a wide range of droplet dimensions and
volumes:

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 9 of 17

 Droplet size Order
uEncapsulator Droplet Chips
(volume) Number
2 Reagent Droplet Chip (50µm etch depth), 3200563
fluorophilic 35 – 70 µm
2 Reagent Droplet Chip (50µm etch depth), (22 – 180 pl)
3200564
hydrophilic
2 Reagent Droplet Chip (30µm etch depth), 3200567
fluorophilic 20 – 40 µm
2 Reagent Droplet Chip (30µm etch depth), (4 – 33 pl)
3200568
hydrophilic
2 Reagent Droplet Chip (15µm etch depth),
3200565
hydrophilic 10 – 20 µm
2 Reagent Droplet Chip (15µm etch depth), (0.5 – 4 pl)
3200566
fluorophilic

9.1 Features and benefits

• Rapid generation of 2 reagent droplets containing biological material

• Fluorophilic surface coating for use with fluorocarbon carrier oils

• Attached FKM 0.5 mm thick gasket for chip-to-chip connection

• Integrated flow resistors for excellent flow stability

• F20* flow resistor on each droplet line

• F600* flow resistor on oil line

• 100 µm scale marks at the junction for imaging

• Fabricated in glass

• Very smooth channels (surface roughness Ra of 5 nm)

• Excellent optical clarity

*F(X) notation signifies the approximate flow rate in µl/min that is obtained when
water is pumped at 1 bar pressure at room temperature and there is no other fluidic
resistance in series.

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 10 of 17

 9.2 Geometry

uEncapsulator Chip Layout

2 Reagent Droplet Chip Junction 2 Reagent Droplet Chip Junction 2 Reagent Droplet Chip Junction
(50 µm etch depth) (30 µm etch depth) (15 µm etch depth)

On-chip filter on droplet lines with On-chip filter on droplet lines with On-chip filter on droplet lines with
pore size 50 µm x 55 µm (depth x pore size 30 µm x 35 µm (depth x pore size 15 µm x 20 µm (depth x
width) width) width)

On-chip filter on carrier oil line with On-chip filter on carrier oil line with On-chip filter on carrier oil line with
pore size 25 µm x 55 µm (depth x pore size 15 µm x 35 µm (depth x pore size 7.5 µm x 20 µm (depth x
width) width) width)

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 11 of 17

10 P-Pump
The Mitos P-Pump provides pulseless liquid flow with
a precise pressure driven pumping mechanism. When
used in conjunction with Dolomite flow sensors, flow
rate as well as pressure can accurately be set and
controlled. The P-Pump operates over a wide pressure
range (0-10 bar) with excellent response time and
accuracy. Pressure driven flow is ideal for microfluidic
systems where highly stable flow is required in the
nl/min to µl/min range for applications such as droplet
formation. The design features a lockable pressure
chamber for safety, which is easy to access and
accommodates a wide range of fluid vessels.

10.1 Features and benefits

• Pulseless pumping performance
• Fast response time
• Wide pressure range (0 - 10 bar) enabling use with systems of high fluidic
resistance
• Flexible - accommodates a wide range of fluid vessels (standard and non-
standard)
• Quick and easy to use
• Works with laboratory N2 or Ar supply, gas bottle or compressor
• Efficient - uses samples directly from your container, avoiding mess and/or
waste

10.2 Specifications

Specification

Weight 2 kg
Ampere 1A
Voltage 110 V
Operating Temperature 5 – 40 ˚C
Operating Humidity 20 – 80 %
239 mm (L) x 93 mm (W) x 146
Dimension
mm (H)

11 Sensor Interphase/ Display

The Mitos Sensor Display can be used interchangeably
with the Mitos Flow Rate Sensors (Part Nos. 3200096-
3200100) which simply attach with a push-click action.
Easy to use, the Mitos Sensor Display provides a USB
connection and visual output for displaying micro-scale
flow rate and pressure.

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 12 of 17

 11.1 Features and benefits
• Real-time display of flow rates
• Easy to use, just connect and switch on
• Dimensions: 20.5mm (length) x 82mm (width) x 55mm (height)
• Interchangeable flow rate sensors to provide wide range of flow rates
• Excellent chemical resistance
• Standalone operation or integrated with a PC or a Mitos P-Pump (Part
No. 3200016)

12 Flow rate sensor

Designed for use with the Mitos Sensor Display (Part
No. 3200095), the Mitos Flow Rate Sensors enable fast
and accurate measurements of ultra-low liquid rates.
The high precision thermal sensor technology provides
total media isolation and extremely low internal volume
with no moving parts.

All measurement data is fully calibrated for water and temperature compensated by
means of an internal microcontroller.

12.1 Features and benefits

• Flow rate range: 30-1000 µl/min, 1 – 50 µl/min, 0.4 – 7 µl/min

• Inner diameter: 1000 µm, 430 µm, 430 µm

• Maximum pressure: 10bar

• Overall size: 75mm (length) x 82mm (width) x 60mm (height)

• Compatible with Mitos P-Pump (Part No. 3200016)

• Wetted materials: PEEK, glass and PTFE

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 13 of 17

13 Microscope Specifications
Specification

Visible area of chip (Innovate Chip Ø 3.5 mm around junction, Ø 5 mm

Interface – Standard Mode) around output

Visible area of chip (Innovate Chip Full channel network 12 mm x 60

Interface – Inverted Mode) mm
Minimum field of view (maximum
0.26 mm x 0.19 mm (approx.)
zoom)
Maximum field of view (minimum 1.8 mm x 1.3 mm (approx.)
zoom)
Objective lens magnification 5X
Zoom ratio 7:1
Working distance 45 mm
Number of pixels per micron at
5
maximum zoom
LED Luminous Flux (1) 4000 lumens
LED colour Cool white
LED wavelength 400 – 800 nm
1.3 Megapixel colour camera, 1/2”
Imaging device
CMOS sensor
Resolution 1280 pixels x 1024 pixels
Minimum Shutter time 0.05 ms

~150 fps @ 1280 x 1024

~506 fps @ 640 x 480
Frame rate (2)
~1,500 fps @ 320 x 240
~4,100 fps @ 160 x 96
Still image file type .bmp
Video image file type .avi
PC connection USB 3.0
260 mm x 318 mm x 490 mm (W x
Module dimensions (3)
L x H)
107 mm x 187 mm x 52 mm (W x L
Stage dimensions
x H)
Supply voltage and frequency 80 – 264 VAC, 47 – 63 Hz
Weight 5 kg
Ampere 1.2 A
Operating Temperature 5˚C – 40 ˚C
Operating Humidity 20 – 80 %

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 14 of 17

14 Temperature Control Unit
The Meros TCU-100 is an advanced temperature
controller for microfluidic chips and other devices. It
can provide extremely accurate control of a fixed
temperature from 1°C to 100°C, rapid temperature
ramps or thermocycling.

14.1 Features and benefits

• Accurate and stable: Stability ± 0.1 °C
• Fast ramp rates: Temperature ramps up to 2 °C/s
• Compact: Only 11 cm (4 ¼") wide
• Flexible: Accepts 100 s of chip designs and other devices
• Easy to use: Plug and play
• Versatile: Can be integrated with a larger microfluidic system, such as
pumps, sensors, valves etc.
• Ideal for academic or industrial applications
• Robust: Chemically resistant and reliable

14.2 Specifications

Specification

Weight 2 kg
Ampere 2.1 A
Voltage 110 V
Operating Temperature 5 – 40 ˚C
Operating Humidity 20 – 80 %
180 mm (L) x 107 mm (W) x 149
Dimension
mm (H)

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 15 of 17

 [Page intentionally left blank]

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 16 of 17

Dolomite Microfluidics Offices
Dolomite Microfluidics - UK (Europe and Rest of World)

Unit 1, Anglian Business Park, Royston, Herts, SG8 5TW, United Kingdom

Telephone: +44 (0) 1763 242 555

E: info@dolomite-microfluidics.com

W: www.dolomite-microfluidics.com

Dolomite Microfluidics - USA

156 Norwell Avenue, Norwell, Massachusetts, 02061, U.S.A.

Telephone: +1 617 848 1211

E: info@dolomite-microfluidics.com

W: www.dolomite-microfluidics.com

Dolomite Microfluidics - Japan

1002 Kannai JS Bldg. 207-2 Yamashita-cho, Naka-ku, Yokohama-shi, Kanagawa, 231-0023

Telephone: +81 452 638 211

E: info@dolomite-microfluidics.com

W: www.dolomite-microfluidics.com

Dolomite Microfluidics - India

607-E, Ecstasy Business Park, City of Joy-Nirmal Lifestyle,

JSD Road, Muland West Mumbai 400080

Telephone: +91 98 9232 7462

E: info@dolomite-microfluidics.com

W: www.dolomite-microfluidics.com

Dolomite Microfluidics - Brasil

Rua Dr. Bacelar, 231 - cj 47, Vila Clementino, 04026-000 / São Paulo – SP, Brasil

Telephone: +55 11 5083 4963

E: info@dolomite-microfluidics.com

W: www.dolomite-microfluidics.com

Dolomite Microfluidics - SHPT-487168127-326_v.5.0 Page 17 of 17