INTERMED Page 1 of 4

SOP No.: IM-II/QM/007

STANDARD OPERATING PROCEDURE
Title: CALIBRATION PROCEDURE FOR
Revision No.: 00
MICROPIPETTES
Department Microbiology Supersedes SOP: NA
Effective Date: Next Review Date:

1.0 PURPOSE:
To describe the standard operating procedure for calibration of micropipettes.

2.0 SCOPE:
This procedure is applicable for calibration of micropipettes used in the microbiology
laboratory.

3.0 RESPONSIBILITY:
3.1 Senior Executive-Microbiologist/ Designee

4.0 ACCOUNTABILITY:
4.1 Head-QC/ Designee
4.2 Head-QA/ Designee

5.0 PROCEDURE:
5.1 Receipt of micropipette:
5.1.1 Whenever a new micropipette is received it should be checked for its make, range that
is specified in the purchase order.
5.1.2 Verify if the supplier’s certificate of analysis is present.
5.1.3 Internally calibrate the micropipette given below the point 5.2 as follows.
5.1.4 After calibration that gives satisfactory result, assign the micropipette a serial number
and other details in the format: IM-II/QM/007/F2.
5.1.5 Assign the micropipette serial number as follows:-QM/MP/XX.
Where,
QM-Quality Control Microbiology,
MP-Micropipette,
XX-Sequential number starting from 01

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Format number: IM-II/QA/001/A2-00
 INTERMED Page 2 of 4
SOP No.: IM-II/QM/007
STANDARD OPERATING PROCEDURE
Title: CALIBRATION PROCEDURE FOR
Revision No.: 00
MICROPIPETTES
Department Microbiology Supersedes SOP: NA
Effective Date: Next Review Date:

5.1.6 Paste the calibration label as per the format: IM-II/QM/007/F3.

5.2 Calibration:
5.3 Clean the external surfaces of Micropipette with clean dry lint free cloth.
5.4 Sanitize the Micropipette with wet cloth dipped with 70% IPA.
5.5 Wipe the Micropipette with dry cloth after sanitization.
5.6 SET the desired volume by adjusting the plunger cap by rotating in clockwise or
anticlockwise direction.
5.7 Keep clean, dry beaker over the balance pan.
5.8 Put the print to tare weight of beaker.
5.9 Fix the Micro pipette with appropriate tip firmly.
5.10 Note the temperature of the purified water used for Performance Verification of
Micropipette.
5.11 Take purified water and adjust its temperature to 25ºC pipette out the purified water.
5.12 Pipette out set volume of purified water and dispense in the beaker.
5.13 Observe the weight and print it then repeat the pipetting and weighing to totally 10
times. From the weight of water calculate the volume as per the following formula.

Weight of Beaker with water (W2) - Weight of empty beaker (W1)

Volume of Purified =
water (V) Density of Purified water

Micro Pipette Range (µL) Set Volume (µL) Number of Transfers

10 10
10 - 100 50 10
100 10
100 10
100 - 1000 500 10
5.14 1000 10
1000 10
1000 - 10000 5000 10
10000 10
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Format number: IM-II/QA/001/A2-00
 INTERMED Page 3 of 4
SOP No.: IM-II/QM/007
STANDARD OPERATING PROCEDURE
Title: CALIBRATION PROCEDURE FOR
Revision No.: 00
MICROPIPETTES
Department Microbiology Supersedes SOP: NA
Effective Date: Next Review Date:

Three point calibrations shall be performed for the variable range pipette. E.g. 0.01ml, 0 .05 ml,
0.1ml range pipette perform calibration at set point 0.1ml, 0.5ml & 1ml.
5.15 Example calculation
Weight of Water = 100.1 mg
Density of Water at 25ºC = 0.997044 g
Weight of water
Volume of purified water =
Density of water

100.1 mg
Volume of purified water at 25ºC =
997.044 mg
Volume of purified water at 25ºC = 100.40 µl
5.16 Recorded the observation in the format: IM-II/QM/007/F1
5.17 Acceptance Criteria: the volume variation should be within ±2% of designated set
volume.
5.18 If any deviation observed during calibration of micro pipette the analyst shall be inform
to supervisor for necessary corrective action.
5.19 Frequency of Calibration: Once in three month or after each breakdown.
5.20 Acceptance Criteria:
Pipettes and Desired Calibration Volume

S. No. Volume of Pipette Desired Calibration Volume

1 10 to 100 µl 10 µl 50 µl 100 µl

Acceptance Criteria 9.8 – 10.2 µl 49 – 51 µl 98 – 102 µl

2 100 to 1000 µl 100 µl 500 µl 1000 µl

Acceptance Criteria 98 – 102 µl 490 – 510 µl 980 – 1020 µl

3 1000 to 10000 µl 1000 µl 5000 µl 10000 µl

Acceptance Criteria 980 – 1020 µl 4900 – 5100 µl 9800 – 10200 µl

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Signature
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Format number: IM-II/QA/001/A2-00
 INTERMED Page 4 of 4
SOP No.: IM-II/QM/007
STANDARD OPERATING PROCEDURE
Title: CALIBRATION PROCEDURE FOR
Revision No.: 00
MICROPIPETTES
Department Microbiology Supersedes SOP: NA
Effective Date: Next Review Date:

6.0 ABBREVIATIONS, ACRONYMS AND SYMBOLS:

6.1 SOP - Standard Operating Procedure.
6.2 µl - Microliter
6.3 mg - milligram

7.0 DEFINITIONS:
7.1 Micropipette is a laboratory instruments designed for measuring small quantities of
liquid for routine analysis.

8.0 LIST OF ANNEXURES:

8.1 For applicable Format/Annexure list refer “List of Enclosures” as per Annexure no.: “IM-
II/QM/007/A1” (For reference of current revision).

9.0 REVISION HISTORY:

Revision Effective Date of Reason for Reference change
No. Superseded SOPs Revision/Review control no.

00 NA New SOP NIL

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Signature
Date
Designation
Format number: IM-II/QA/001/A2-00