Comparative study of antioxidant activity of orange peel

and frankincense oil in pure form and in antiaging cream.

FRANKINSCENSE TREE TEARS
 Boswellia sacra

 Boswellia carterrii

 Boswellia frereana

 Boswellia seratta

 Boswellia peprifera

 (75% monoterpenoles+ 25% sesquiterpenols & ketones)

 Orange peel oil and frankincense oil are popular for
antiaging creams
 Comparative study to analyze which one is better and
to what extent
 Their activity comparison in pure form and also in
cream base
 ORANGE PEEL AS ONE OF THE BEST
ANTIAGING ANTIOXIDANT
 Rich in d-limonene(cyclic mono terpenes)

 Very high antioxidant activity(scavenge free radicals)

 Support cellular functioning

 By product of orange juice production

 EXTRACTION OF PURE OIL BY STAEM
DISTILLATION
 Staem distillation for both essential oils extraction

 Repeat distillation many times for appropriate

amount
 Ingredient Percentage
Parrafin oil 3
Bee wax 7
Emulsifying wax 7
Cetyl alcohol 3
water 49
Glycerine 1
Active agent (antiaging oil) 3
Physical Properties of anti-aging Cream

Initial After 10 days at After 10 days at

Sr. no. Properties
-20 oC 60 oC
1 Colour White White White
Characteristic Characteristic/ no
2 Odour Characteristic
smell
3 Appearance Semi -solid Semi -solid Semi-solid

Thermal stability and pH Determination

Sr.No. TEST After 10 days at -20 oC After 10 days at 60 oC

Initial
Thermal Stability (at Stable, no oil
Semi -solid,
1 RH 75% Stable, No gelling separation/ no
Emulsion
and 60 ± 2 oC) deterioration

2 pH (at 27oC ± 2oC) 6.03 5.58

6.06

3 Texture ok ok
ok

4 Degradation of product Nil Nil

Nil
 FRAP Assay
20
19.5
19
FRAP Value (µM)

18.5
18
17.5
17
16.5
16
15.5
15
Or-1 F-oil
DPPH ASSAY AND RESULTS. Absorbance measured at
515 nm
Mathonal
n-Hexane Layer (absorbance)
(absorbance)

Test-1 Test-2 Test-3 Avg. Value Test-1 Test-2 Test-3 Avg. Value
F-1 1.02 1.03 1.046 1.032 0.655 0.732 0.6 0.662
Frankincense Oil F-2 0.669 0.667 0.669 0.668 0.63 0.632 0.74 0.667
F-3 0.607 0.602 0.601 0.603 0.582 0.62 0.614 0.605
O-1 0.5 0.73 0.81 0.680 1.192 1.28 1.16 1.211
Orange Peel Oil O-2 0.62 0.52 0.61 0.583 1.58 1.64 1.64 1.620
O-3 0.22 0.2 0.22 0.213 1.68 1.66 1.36 1.567
BK-1 0.91 0.89 0.95 0.917 0.67 0.69 0.6 0.653
Cream-A BK-2 1.11 1.12 0.99 1.073 0.65 0.65 0.65 0.650
BK-3 0.95 0.89 1.03 0.957 0.71 0.65 0.65 0.670
O-1 0.694 0.755 0.801 0.750 0.728 0.735 0.74 0.734
Cream-B O-2 0.691 0.7 0.99 0.794 0.69 0.69 0.69 0.690
O-3 0.725 0.82 1.03 0.858 0.733 0.731 0.73 0.731
FC-1 0.88 0.94 1.02 0.947 0.88 0.87 0.88 0.877
Cream-C FC-2 0.96 0.91 1.02 0.963 0.81 0.81 0.81 0.810
FC-3 0.99 0.93 0.99 0.970 0.703 0.68 0.68 0.688
BD-1 0.78 0.823 0.98 0.861 0.7 0.694 0.687 0.694
Cream-D BD-2 0.83 0.79 0.829 0.816 0.68 0.68 0.68 0.680
BD-3 0.78 0.79 0.79 0.787 0.66 0.67 0.66 0.663
 Absorbance measured at
760 nm

n-Hexane Layer Mathonal

(absorbance) (absorbance)
Avg. Value Avg. Value
0.5 ml 0.85
Frankincense Oil 1 ml 0.80
1.5 ml 1
0.5 ml 0.85
Orange Peel Oil 1 ml 1.53
1.5 ml 1
BK-1 0.77 0.7
Cream-A BK-2 1.41 0.47
BK-3 2.21 0.42
O-1 1.77 0.52
Cream-B O-2 2.55 0.41
O-3 2.65 0.38
FC-1 0.74 0.43
Cream-C FC-2 0.936 0.41
FC-3 2.61 0.46
BD-1 0.96 0.51
Cream-D BD-2 2.25 0.37
Peak Base Base
R.Time I.Time F.Time Area Area% Height Weight% A/H Mark
# m/z Int.
1 7.112 7.067 7.167 2136905 31.88 962107 38.24 2.22 V 93.10 235936
2 7.332 7.300 7.383 457610 6.83 213731 8.50 2.14 V 93.10 46272
3 8.598 8.550 8.650 370749 5.53 156250 6.21 2.37 93.05 33578

4 10.301 10.250 10.367 567555 8.47 204031 8.11 2.78 119.05 60134

5 20.454 20.367 20.667 1206728 18.00 143718 5.71 8.39 V 32.10 9001

6 26.750 26.733 26.825 243855 3.64 105891 4.21 2.30 V 43.10 3808

7 28.050 28.008 28.125 297353 4.44 103903 4.13 2.86 74.05 24349

8 29.991 29.958 30.025 249763 3.73 126293 5.02 1.97 67.10 9872

9 30.059 30.025 30.100 752749 11.23 373819 14.86 2.01 V 55.10 27842

10 30.906 30.833 30.958 419224 6.25 126004 5.01 3.32 149.05 23918
6702491 100.00 2515747 100.00