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Experiment 3.

3
DETERMINING THE CONCENTRATION OF AN
EXTERNAL SOLUTION WHICH IS ISOTONIC
TO THE CELL SAP OF PLANT CELLS
Determining the Procedure :
concentration of an external 1. Prepare and label seven petri dishes A, B, C, D,
solution which is isotonic to E, F and G.
the cell sap of plant cells 2. Fill each beaker with the solutions in the table
Problem statement : __________ below.
Variables : 3. Remove the potato tissue from the cork borer.
Manipulated variable : _________ 4. Then cut the potato into a cylindrical strip
Responding variable : _________ 50mm long.
Fixed variable : _________ 5. Repeat step 3 to 5 to prepare another six
Hypothesis : _______________ cylindrical strips of the same length.
Materials : a freshly cut potato,
6. Wipe dry each strip with a piece of tissue paper.
distilled water, 0.1M, 0.2M, 0.3M,
0.4M, 0.5M and 0.6M sucrose 7. Weigh and record the mass of each potato strip.
solution. 8. Then place each of a potato strip in a petri dish.
Apparatus : a razor blade, a cork 9. Cover completely the strips of potato in the
borer, Petri dishes, forceps, 50ml
solution.
beaker, an electronic balance and
tissue paper. 10. After soaking for an hour, remove each strip
Technique : Measure the mass of from its respective petri dish and wipe dry.
potato strips with an electronic Weigh again and record the mass of each strip.
balance and determine the 11. Record the result in a table. Draw a graph of the
percentage difference in mass. percentage difference in mass against the
concentration of the sucrose solution.
Result :

Solution Petri Mass of the strip Difference Percentage difference Texture and
dish of potato (g) in mass in mass (%) appearance
Distilled A
water
0.1M sucrose B
solution
0.2M sucrose C
solution
0.3M sucrose D
solution
0.4M sucrose E
solution
0.5M sucrose F
solution
0.6M sucrose G
solution
Graph of percentage difference in the Discussion :
mass of potato strips against the
concentration of sucrose solution

Percentage
difference in
mass (%)

Concentration
of sucrose
solution (M)

Conclusion :

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