Professional Documents
Culture Documents
FS0102 1 2000
Microorganisms classified
by their significance
pathogenic organisms
spoilage organisms
useful organisms
FS0102 2 2000
Dangerous microorganisms
and parasites
Foodborne diseases
bacteria
moulds
viruses
parasites
FS0102 3 2000
Major bacteria causing
foodborne disease
Aeromonas spp. Mycobacterium bovis
Bacillus cereus Salmonella spp.
Brucella spp. Shigella spp.
Campylobacter jejuni Staphylococcus aureus
Clostridium botulinum Vibrio cholerae
Clostridium perfringens Vibrio parahaemolyticus
Escherichia coli Vibrio vulnificus
Listeria monocytogenes Yersinia enterocolitica
FS0102 4 2000
Spoilage microorganisms
bacteria
yeasts
moulds
FS0102 5 2000
Food products made
with useful microorganisms
fermented meats
yoghurt
cheese
beer
leavened bread
soy sauce
fermented soybean (tofu)
FS0102 6 2000
Useful microorganisms
1 cup
of yoghurt contains
20 X this number!
World population is i.e. 120 000 000 000
5.9 BILLION separate living
organisms.
i.e. 5 900 000 000
FS0102 8 2000
Microorganisms are very small
Cell wall Cytoplasmic
membrane
Ribosomes Cytoplasm
Nuclear
FS0102 9 2000
body
Bacterial division
5
FS0102 10 2000
Bacterial growth curve
Toxic
- - - - - - - - - - - -+++++++++++
_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _
Spoils
+ + +
9
Time to spoilage
8
7
6
5
4
3
2
1
0
Time
Lag phase Log phase Stationary phase
FS0102 11 2000
Phases of bacterial growth
Lag phase
(short) period of adjustment to environment
Logarithmic Growth phase
growth begins and accelerates to a phase of rapid,
constant exponential growth
Stationary phase
depletion of nutrients and accumulation of toxic
metabolic products growth is slowed to a point
where cell division and cell death are in balance
Death phase
population decreases due to death of cells
FS0102 12 2000
Some toxigenic moulds
causing foodborne disease
Aspergillus spp.
Fusarium spp.
Penicillium spp.
FS0102 13 2000
Major viruses
causing foodborne disease
Rotavirus
Polio virus
FS0102 14 2000
Virus structure
Protein Capsule
Poliovirus
Hepatitis-A virus
Rotavirus
Norwalk-like or
SRSV
Nucleic acid
(DNA or RNA)
FS0102 15 2000
Virus levels required for
infection and detection
infection
10 -100 particles / ml
detection
105 - 106 particles / g
(by electron microscopy)
104 - 105 particles / g
(by radioimmunoassay)
FS0102 16 2000
Examples of peak excretion period
of some faecal viruses
SRSV 1 - 3 days
Astrovirus 1 - 4 days
Rotavirus 1 - 7 days
Adenovirus prolonged
Coronavirus prolonged
cause diarrhea
Small round structured viruses (SRSV)
Astrovirus
Adenovirus
Coronavirus
Rotavirus
do not cause diarrhea
Hepatitis A virus
Poliovirus
Echovirus
Coxsackievirus
FS0102 18 2000
Major parasites
causing foodborne disease
Protozoa
• Cryptosporidium
• Entamoeba histolytica
• Giardia
• Toxoplasma
Helmints
• Angiostrongylus • Fasciola
• Anisakis • Fasciolopsis
• Ascaris • Haplorchis
• Capillaria • Opisthorchis
• • Paragonimus
Gnathostoma
• Cysticercus cellulosae
• Trichinella
FS0102 19 2000
Transmission of trichinellosis
Infected person
RAT
PIG
encysted
larvae
in muscle Adults in encysted larvae
intestine in muscle
FS0102 20 2000
Transmission of cysticercosis
Infected person
Cysticercus cellulosae
in the body
Taenia saginata
Regurgitating
Taenia solium
Adult in intestine
Contaminated
vegetable, water
Eggs in soil
FS0102 21 2000
Transmission of toxoplasmosis
Cat
Infected person
Bradyzoite Oocyst
in meat
ingestion
Transplacenta Mammals
transmission Birds
(Cattle, pig
goat etc.)
handling
Baby
meat
Meat
FS0102 22 2000
Transmission of anisakiasis
Infected person
Adult
in
Marine mammals
2nd stage larvae
Fish
encysted larvae
FS0102 23 2000
Transmission of ascariasis
Infected person
Adult worms
in intestine
Contaminated
hand Contaminated
vegetables
with night soil
Unsegmented Infective
eggs excreted eggs
FS0102 24 2000
Transmission of fascioliasis
Infected person
Sheep
Juvenile migrate
to other organs ie.
brain, eye, muscle
FS0102 25 2000
Transmission of giardiasis
Infected person Giardia cysts in intestine
Cyst in water
Cysts on hands
Cyst
FS0102 26 2000
Transmission of cryptosporidium
The Environment
Effluents, slurry & sludge Liquid discharges and
dumped on land effluents dumped in water
Water treatment
system
Farm
Animals
Human
Imported
exotic
Indigenous livestock
wildlife Pets
FS0102 27 2000
Nature of bacteria, moulds, viruses
and parasites - Key messages (1)