Formalin-ethyl Acetate

Concentration, FLOTAC
Pellet And Anal Swab
Techniques For The
Diagnosis Of Intestinal
Parasites

Mulyanti

Source: CDC, 2020

 FLOTAC
vs

Aim FECT
Diagnosis of intestinal parasites
INTRODUCTION
Intestinal parasitoses caused
by protozoa
and helminths represent a
public health problem and
affect millions of people
worldwide
Feccal
contamination,
inadequate
hygiene
 Coproparasitological
Methods
A diphasic
sedimentation method
 diagnosis of Anal
parasites from fixed FECT swab

samples
Centrifugation in a
Qualitative flotation solution
FLOTAC (FS)

Quantitative
Materials and
methods
 Study Area
May 2017

Puerto Madryn (province of

Chubut, South Argentina)

<14 yo 174
 8

Study Population
May 2017

174 samples 5 municipal Child

Development Centers
≤ 14 yo : (CDC):N°1,N°2,N°
3 yo (31%) 3,N°4 and N°8
4 yo (28,2 %)
5 yo (11,5%)

89 (51,1 %) 85 (48,9 %)
Sample Collection And Parasitological
Analysis
Processed
Informed consent FLOTAC & FECT
Parents and legal guardians

Samples Examination
Same experienced
2 vials for stool parasitologist
samples and anal
swabs
Preservativ
e Data analysis
Formalin 5% software R
Procedure
 The Formalin-ethyl Acetate
Concentration Technique(FECT)

Stool samples

Shaken Examined
vigorously and the pellet
centrifuged

10 ml Pellet +
suspension, 7 ml formalin
centrifuged, +
supernatants 3 ml ethyl
were acetate
discarded Recommende
d by WHO
 FLOTAC
Stool samples

centrifuged ,
weighed the supernatants were
pellet (0,3 g) discarded
12 ml suspension,
centrifuged,
supernatants were
discarded 12 ml water
6 ml 6 ml

Homogenised

6 ml FS2 6 ml FS3
 Anal Swab

Stool samples

15ml

Agitated centrifuged 10
vigourously min at 400g

E. vermicularis
 Stastistical Analysis

Sensitivit
y
Negative Kappa
Predictiv
e Value
Index
(NPV) (KI)
 KAPPA INDEX
MODERA
TE
POOR
≤0 0,41-0,60
SLIG SUBSTANTI
HT AL
0,01-0,20 0,61-0,80
KI

ALMOST
FAIR
PERFECT
0,21-0,40
0,81-1,00
Results
Kappa index
Agreement
FECT and
FLOTAC
 CONCLUSION
FECT and FLOTAC  recommended as
complementary copromicroscopic methods 
diagnosis of intestinal parasites in humans

FECT  reliable method for detecting protozoa

FLOTAC  importance in the diagnosis of

helminths in populations where the prevalence is low
Thankyou…