Professional Documents
Culture Documents
By
Trishla Chaturvedi
''How can I have possibly come so far,
And still have so far to go ? ''
HAEMOCYTOMETER /
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Automated Blood Cell Counter
Automated Blood Cell Counter
Sysmex XT i2000
CelI DYN Ruby
■ Automation minimizes or does away with the need for
manual intervention.
■ Even semi-automation involves steps like the dilution
of blood samples and allows for the measurement of
only fewer variables.
■ With a fully automated system, all that is required is
an appropriate blood sample.
■ Errors are a major issue with manual counting. There
are problems with cell recognition such as
distinguishing lymphocytes from monocytes and these
may be overestimated or underestimated.
■ Other sources of error with manual counts pertain to
distribution of cells on the slide and statistical
sampling error. One the other hand, automated cell
counters ensure a high level of precision and accuracy
for cell sizing.
■ Automated leukocyte differentials significantly reduce
the time and cost of performing routine examinations.
State of the Art Hematology Analyzers
■ Hematology analyzers also come with on
board storage for thousands of patient reports
and even allow for customization of these
reports with patient identification and color
histogram.
Advantages of Automated Hematology Analyzers
►Impedance (conductivity)
System
(coulter)
►Optical System (Light
Scattering)
►Flow Cytometry
►S elec ti ve Lysis
►Special Stains
Analytic Goals
■ CBC Count
Leukocytes
Erythrocytes
Hemoglobin
Mean cell volume
Platelets
■ Leukocyte
Differential Count
Neutrophils
Lymphocytes
Monocytes
Eosinophils
Basophils
■ Reticulocyte
Whole Blood Reportable Parameters
►C WB
►C RB
►B HG
►CTH
►C MV
M
Leukocyte Differential Count
►hemodialysis
Guidance of iron and EPO therapy in
►withDiagnosis of iron deficiency in patients
patients
Blood films
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Blood Cells
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• • •• •••••• • •• • •
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Erythrocytes
..
Eosinophil
Monocyte
Platelets
••
•• • •
•
• •• •
Ly m p h o c y t e • ••
Neutrophil
•
••
Basophil
Peripheral Blood Film (Typical)
Peripheral Blood Film (Atypical)
' Peripheral Blood Film (Atypical)
•!• technological evolution as applied to hematology
Theanalyzers has provided new opportunities.
•!• The possibility of determining the fraction of immature
platelets by using a simplified method opens the door to new
applications.
•!• It should be remembered that despite the essential role of
automation in the modern hematology laboratory, microscopic
control of pathologic samples remains indispensible, so much
so that in certain cases, it alone is diagnostic.
•!• Moreover, knowledge of the limits of the specific analyzer in
use is of paramount importance for the correct interpretation of
results.
Thanks To All
For Patient Hearing