Axolotl- Skin Regeneration

EPIDERMIS
Axolotl skin BASEMENT MEMBRANE
DERMIS

Incision is made.

1hr after injury.

some epidermal cells at the edge of the wound
Regeneration Process:
are already positioning themselves in
1. End of Blood Flow
The axolotl is a Urodele amphibian. preparation for migration over the wound bed.
2. Inflammation
It can regenerate multiple structures like 3. New tissue formation
limbs, jaws, tail, spinal cord, skin and more 4. Remodeling process
without evidence of scarring throughout
their lives.  4hrs later 
Axolotls can even receive transplanted Epidermal cells have already migrated to
organs from other individuals and accept cover about half the distance to the center
them without rejection. of the wounded area.

Complete wound closure by 8hrs

after wounding.

Design Workshop I Year 02 Shravani Salvi B029

 Basement Membrane Regeneration
Basement Membrane
Regeneration completed after
90 days.

The wound epidermis has

covered the wound bed but is
still not lying directly on the
muscle 

1hr

4hrs

Even after 8hrs of wound enclosure no

8hrs basement membrane regeneration is seen.

Design Workshop I Year 02 Shravani Salvi B029

 Skin Details:​ • Fibroblasts migrate to the
•Epidermis contains keratinocytes which aid in actively dividing to provide new wound bed and proliferate.
cells to replenish lost skin.​ (division of cells)
• Fibroblasts secrete ECM
•Dermis consists of a loose network of thin collagen fibres and fibroblasts.​

• Fibroblasts migrate through the

collagen network which leads
to collagen network tightening.

• Fibroblasts get activated and

form myofibroblasts

• Myofibroblasts take part in

wound contraction
• Fibroblasts transform and remodel ECM
to Myofibroblasts- helps in wound repair by
contracting the edges of the wound.

Fibroblasts Myofibroblasts

Design Workshop I Year 02 Shravani Salvi B029

 Collagen Fibers

Strain produced 

Cell-induced Matrix Reorganization

Design Workshop I Year 02 Shravani Salvi B029

 2nd Attempt
1st Attempt

Fixed points

3rd Attempt

Fixed points

Design Workshop I Year 02 Shravani Salvi B029

Fixed points

Design Workshop I Year 02 Shravani Salvi B029

Design Workshop I Year 02 Shravani Salvi B029
 1st unit attempt 2nd unit attempt

Design Workshop I Year 02 Shravani Salvi B029

 Final Model

Design Workshop I Year 02 B029 Shravani Salvi 

 Final Model

Design Workshop I Year 02 B029 Shravani Salvi