Litopenaeus vannamei (Pacific

white shrimp) Shells and

Decapodiformes (Squid) Pens
as Biodegradable Plastic

Murray, Gortayo, De La Cruz

CHAPTER 1
Introduction
Versatile
material

Major
problem
CHITIN
is a polysaccharide

Shells of shrimps
Pens of squids
Statement of the Problem
1. What are the properties of biodegradable plastic made of Litopenaeus
vannamei (Pacific white shrimp) Shells and Decapodiformes (squid) pens,
biodegradable plastic made of cassava starch, and non-biodegradable
plastic
in terms of:
a. Tensile Strength
b. Density
c. Water Permeability
d. Biodegradability
i. Photo Degradability
ii. Soil Degradability
 
Statement of the Problem
2. Is there a significant relationship between the amount of chitin in grams
and the following properties of the biodegradable plastic:

a. 10g vi. Density

i. Tensile Strength vii. Water Permeability
ii. Density viii. Biodegradability
iii. Water Permeability a) Photo
iv. Biodegradability Degradability
a) Photo b) Soil Degradability
Degradability
b) Soil Degradability
Statement of the Problem
2. Is there a significant relationship between the amount of chitin in grams
and the following properties of the biodegradable plastic:

c. 30g
i. Tensile Strength
ii. Density
iii. Water Permeability
iv. Biodegradability
a) Photo Degradability
b) Soil Degradability
Statement of the Problem
3. Is there a significant difference in the properties of biodegradable plastic made of
Litopenaeus vannamei (Pacific white shrimp) Shells and Decapodiformes (squid)
pens, biodegradable plastic made of cassava starch, and non-biodegradable plastic
in terms of the following:
a. Tensile Strength
b. Density
c. Water Permeability
d. Biodegradability
i. Photo Degradability
ii. Soil Degradability
Hypothesis

HYPOTHESES
1. There is no significant relationship between the amount of chitin in grams and the following properties
of biodegradable plastic:
a. 10g c. 30g
i. Tensile Strength i. Tensile Strength
ii. Density ii. Density
iii. Water Permeability iii. Water Permeability
iv. Biodegradability iv. Biodegradability
a.) PhotoDegradability a.) Photo Degradability
b.)Soil Degradability b.)Soil Degradability
b. 20g
i. Tensile Strength
ii. Density
iii. Water Permeability
iv. Biodegradability
a.)Photo Degradability
b.)Soil Degradability
Hypothesis

HYPOTHESES
2. There is no significant difference in the properties of biodegradable plastic made
of Litopenaeus vannamei (Pacific white shrimp) Shells and Decapodiformes (squid)
pens, biodegradable plastic made of cassava starch, and non-biodegradable plastic
in terms of the following:
a. Tensile Strength
b. Density
c. Water Permeability
d. Biodegradability
i. Photo Degradability
ii. Soil Degradability
Conceptual Framework
Types of plastic Properties
A. Non-biodegradable plastic A. Tensile Strength
B. Cassava plastic B. Density
C. Biodegradable plastic made C. Water Permeability
of chitin D. Biodegradability
• 10g • Soil Degradabilty
• 20g • Photo Degradability
• 30g

Figure 1. The figure shows the relationship between the types of plastic and its properties
Scope and Limitations
Significance of the Study
CHAPTER 3
Materials and Methods
Research Design True Experimental

Gathering of Pacific Gathering of

Gathering of non-
white shrimp shells and biodegradable plastic
biodegradable plastic
squid pens made up of cassava starch
(1 day)
(1 day) (1 day)

Preparation of Pacific white shrimp

shells and squid pens
(2 days)

Pulverization of samples
(2 days)

Purification of chitin
(1 week)

Evaluation of chitin
(1 day)
 Formulation of biodegradable plastic
a. 10g
b. 20g
c. 30g
(1 week)

Test for different properties

a. Tensile Strength (1 day)

b. Density (1 day)
c. Water Permeability (1 day)
d. Biodegradability
i. Photo Degradability (3 weeks)
ii. Soil Degradability (3 weeks))

Analysis of data
(2 days)

Figure 2. The flowchart shows the experimental processes needed to conduct this study
Location and Duration of the
Study
Tatoy’s Manokan and Seafood Restaurant
and Elizabeth Restaurant

Chemistry Laboratory of Assumption-Iloilo

University of San Agustin

October 18, 2019

Materials and Methods
SQUID PENS AND SHRIMP SHELLS

250g 250g 1hr, 110°C

Materials and Methods
PULVERIZATION OF SAMPLES

Powdered
samples
Materials and Methods
CHEMICALS

Patagonian Enterprises
Materials and Methods
PURIFICATION OF CHITIN
Demineralization
Materials and Methods
PURIFICATION OF CHITIN
Deproteinization
Materials and Methods
PURIFICATION OF CHITIN
Decolorization
Materials and Methods
EVALUATION OF CHITIN
 Materials and Methods
POLYETHYLENE PELLETS

BIODEGRADABLE PLASTICS

NON-BIODEGRADABLE
PLASTIC
Materials and Methods
FORMULATION OF BIODEGRADABLE PLASTIC
 Materials and Methods
DATA COLLECTION
Tensile Strength:

Density:

Density =  mass / volume  ( cm 3 )

Soil Degradability:

1 ft . 3 weeks
 Materials and Methods
DATA COLLECTION
Photo Degradability:

3 weeks

Water Permeability:

15ml  [( wet   weight − dry   weight ) /  wet   weight ]  x  100%
Materials and Methods
DATA ANALYSIS

One-way ANOVA
Analysis of Variance
Analysis of Covariance

ETHICS

SAFETY CONSIDERATIONS
Materials and Methods
PROPER WASTE DISPOSAL
Data Collection and Analysis
Research Question 1
Data Table 1
Cassava Plastic Bioplastic made of Non-biodegradable
Chitin Plastic

Tensile Strength
(N/cm2)

Density (g/mL)

Water Permeability
(%)

Soil Degradability
(g)

Photo Degradability
(g)
Data Collection and Analysis
Research Question 2
Data Table 2
Cassava Plastic Bioplastic made Non- Mean
of Chitin biodegradable
Plastic

Tensile Strength
(N/cm2)

Density (g/mL)

Water
Permeability (%)

Soil
Degradability (g)

Photo
Degradability (g)
Data Collection and Analysis
Research Question 3
Data Table 3
Cassava Plastic Bioplastic made Non- Level of
of Chitin biodegradable Significance
Plastic

Tensile Strength
(N/cm2)

Density (g/mL)

Water
Permeability
(%)
Soil
Degradability
(g)
Photo
Degradability
(g)