Hematology Analyzer Z3

A new generation 3-diff hematology analyzer

Hematology Analyzer Z3
●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●●

A new generation 3-diff hematology analyzer

contents
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01 Basic Knowledge

02 Z3 Overview

03 Analysis Principle

04 Installation

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contents
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05 Maintenance

06 Mechanical structure

07 Hardware and Hydraulic System

08 Trouble Shooting

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01
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Basic Knowledge

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 Blood Constituent

Include inorganic compound,

50-60% glucose, protein, lipid etc.

0-10%

40-50%

 Blood consists of 40-50% blood cell and 50-60% plasma.

 Three main parts of blood cell: red blood cell, white blood cell and platelet.
 3 Main Blood Cell

Blood corpuscle sort and modality

WBC RBC PLT

Lifespan: 7days 120days 8days

Size: (50-300) fl (60-120) fl (2-30) fl
Ratio: 1 ≥500 ≥30
Function: Anti-infection carry oxygen 　　　 coagulation
　 Immunization metaboly hemostasis
 3-part leukocyte

Morphology: size, shape, cytomembrane, cytoplasm, nucleus, granule.

Lymphocyte Monocyte Eosinophil Basophil Neutrophil

nucleus dark granules

nucleus eosin granules
nucleus nucleus
nucleus

Smallest size, large
nucleus/cytoplasm ratio,
clear cytoplasm, major
constituents of human
immune system.
(No granules)
25 ~ 30%
Largest size, clear
cytoplasm, involved in
ingestion of foreign
substances such as
bacterial or parasites.
(No granules)
3 ～8%
Granules in cytoplasm, Least common, Granules Granules in cytoplasm,
stained in dark red, in cytoplasm, stained in stained in neutral pink,
responsible for dark blue, important react within an hour of
combating infection source of histamine and tissue injury and are
and parasites in cytokine. the hallmark of acute
vertebrates; inflammation;
0.5 ～ 3% 0 ～1% 50 ~70%

Middle Cells
Granulocyte
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Z3 Overview

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 Z3 Overview —appearance

Z3-3 parts hematology analyzer

 Z3 Overview —appearance

touch screen

sampling probe
start key [Aspirate]
power indicator
 Z3 Overview —appearance

4 USB ports

LAN port
Power socket & switch
Waste sensor connector

Waste tube connector

Diluent tube connector

 Z3 Overview —appearance

Built-in thermal printer

Lyse house
 Z3 Overview — Report parameter

Parameters Abbr. Parameters Abbr.

Red Blood Cell Distribution Width -

White Blood Cell count WBC RDW-CV
Coefficient of Variation
8items
Red Blood Cell Distribution Width -
Lymphocytes number Lym# RDW-SD
Standard Deviation
7items Mid number Mid# Hematocrit HCT

Granulocyte number Gran# Platelet count PLT

Lymphocytes percentage Lym% Mean Platelet Volume MPV

Mid percentage Mid% Platelet Distribution Width PDW

Granulocyte percentage Gran% Plateletcrit PCT

6items

Red Blood Cell count RBC Platelet larger cell ratio P-LCR

Hemoglobin Concentration HGB Platelet larger cell count P-LCC

Mean Corpuscular Volume MCV White Blood Cell Histogram WBC Histogram

Mean Corpuscular Hemoglobin MCH Red Blood Cell Histogram RBC Histogram

Mean Corpuscular Hemoglobin

MCHC Platelet Histogram PLT Histogram
Concentration
 Parameters calculated

Abbr. Name Formula

HCT Hematocrit RBC × MCV ÷ 10

MCH Mean corpuscular hemoglobin HGB ÷ RBC

MCHC Mean corpuscular hemoglobin concentration HGB ÷ HCT × 100

PCT Plateletcrit PLT × MPV ÷ 10000

 Background range

Parameter Background

WBC ≤0.2×109/L

RBC ≤0.02×1012/L

HGB ≤1 g/L (0.1g/dL)

HCT ≤0.5%

PLT ≤5×109/L
 Good precision - stable results

Parameter (Detection limits) Whole blood precision (CV)

WBC (7 - 15.0  109/L) ≤2.0 ％

(4– 6.9.0  109/L) ≤2.5 ％

RBC (3.5 - 6.5  1012 / L) ≤1.5%

HGB (110 - 180 g/L) ≤1.5%

MCV (70 - 110 fL) ≤0.5%

PLT (100 - 149  109 / L)

≤5.0%
(150 - 500  109 / L) ≤4.0%
 Wide linearity range

Parameter Linearity range

WBC (0.00 ～ 300.00)×10⁹/L

RBC (0.00 ～ 8.00)×1012/L

HGB 0 ～ 250g/L

PLT 0 ～ 4000×10⁹/L

HCT 0~67%
Z3 Overview-Main Interface

21 parameters

3 histograms + Flag
 Z3 Overview — Specification

Parameters 21parameters

Histograms 3 histograms (WBC 、 RBC 、 PLT)

Test speed 70test/H

Sample mode Whole blood, Pre-diluted

Whole blood: 10ul Pre-diluted: 183ul
Sampling volume
(2-3ml venous blood) (20ul Capillary blood)
Sample storage ≥40000

Screen size 10.4 inch touch color display

Size (mm) 300(W)*400(D) *410(H)

Weight 18Kg

Operating temperature 15℃~35℃

Storage temperature -10℃~40℃

AC input 100V~240V ， 50/60Hz

Power ＜ 200VA
 Easy operation and maintenance

 One button to automatically sample count

 Daily maintenance is performed simply by soaking of probe cleanser during

shutdown.
 Sample probe anti-block design

Bottom is close,
aspiration hole is on aside.
 Long reagent expiration date

Reagent DILUENT LYSE Probe Cleanser

Shelf life 24 months 24 months 12 months

Open-vial stability 60 days 60 days 60 days

 2 years expiration date for diluent & lyse.

 Long expiration date can save the cost for reagent storage.
 Easy troubleshooting

 One button to troubleshoot problem. (Intelligent Fault Diagnosis System)

One button to
troubleshooting
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Analysis Principle

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 Analysis Principle-blood cell counting

Principle ： Electrical impedance method-blood cell counting.

U=R*I
 Analysis Principle-blood cell counting

Principle ： Electrical impedance method-blood cell counting.

 Analysis Principle-blood cell counting

WBC chamber: analog signal Digital signal

RBC are hundreds of times more than WBC, and some parts of WBC are in the same size
as RBC, so the purpose of adding lyse, is to dissolve the RBC.
Meanwhile, PLT still exist in WBC chamber, but signal of PLT is very weak, easy to be
affected by broken RBC pieces, that’s why WBC chamber don’t test the result of PLT.
 Analysis Principle-blood cell counting

RBC signal
RBC chamber: analog signal Digital signal

When test RBC and PLT, WBC signal is also included. WBC result have been tested in WBC
chamber, so software can easily calculate precise RBC result.

PS: 1. RBC and PLT are tested in the same counting chamber.
2. WBC and HGB are tested in another counting chamber.
 Analysis Principle--HGB measurement

Principle ： Colorimetry
Lyse HGB
(Dissolved) HGB React
compound LED530nm

Colorimetry: C = F × A
HGB (C) = constant factor × lg (Blank light intensity / sample light intensity)

Sample in WBC counting chamber.

Signal: 1. Blank light intensity (Colorless Diluent in chamber)
2. Sample light intensity (Red color sample solution in chamber)
 Function of diluent and lyse

Dilute blood cells and

make an even distribution.

Act as a good conductor in Dissolve RBC, then release

the electric circuit. and react with HGB.
Diluent
Used in some maintenance
Lyse
procedures like unclog, and Shrink white blood cells and
cleaning. keep them in a stable status.

Provide an isotonic
environment for blood cell
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Installation

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 Installation – preparation

• Tools needed ：
 Diagonal pliers
 Cross screwdriver
 Calibrator & QC (normal)
 Blood sample Diagonal pliers Cross screwdriver

Installation Video
Calibrator & QC Blood sample
 Installation - preparation

• Environment ：
 Good power grounding;
 Should be free from dust, mechanical vibrations and electrical interference;
 Far from brush-type motors, flickering fluorescent lights;
 Do not place the analyzer on a slope;
 Do not place the analyzer in direct sunlight.
 The environment shall be well ventilated.
 Temperature: 15-30℃.
 Humidity: 20%-85% (operation status).
 Atmospheric pressure: 70-106KPa (operation status).
 UPS is suggested if the power usually shutdown.
 Power stabilizer is suggested if power voltage is unstable.
 Installation - Appearance checking

 Check the appearance of the packaging box, if there are any destroy or deformation for packaging box or not. If
have, please take a photo and send to assigned service engineer of our company. After appearance checking,
then remove the packing string , then open the carton box.
 Installation - unpacking

Accessary box

Documents Main Unit

Waste container
 Take out the instrument from the cargo box, Place the analyzer to flat workbench by handling left &
right side.
 Check the appearance of the analyzer main unit, if there are any destroy or deformation for main unit
or not. If have, please take a photo and send to assigned service engineer of our company.
 Installation – Space & Power input requirement

 Space: Shown as in figure

 Ensure enough space or countertop to
10cm
accommodate the diluent and waste containers.
 Power input requirement:
 Voltage: 100V-240V AC, 50Hz/60Hz
 Input power: ≤200VA
30cm
 Product Standard configuration:
Analyzer, reagents
 Installation – unpacking

Remove fixing belt

Remove 2 fixing belts

Remove
the buffer

Remove 2 shielding paper

 Open the right panel, cut off and remove the fixing belt, which fixes sampling system. (Don’t damage the black
conveyor belt.) Remove the black buffer which between the horizontal motor and pathway. Then, remove the
shielding paper from chambers shielding box.
 Installation - unpacking

Remove notice label on the power socket Connect the power cord

 Remove the Notice label on the power socket and connect the power cord. Ensure the grounding is good. If the
grounding is not good enough (Grounding wire and Null wire voltage is higher than 5V AC), please connect the
grounding wire for the instrument.
 Fix grounding wire

 One end of the grounding wire connects to the device as above

figure shows. Another end goes through the hole at the bottom
and connects to the building’s grounding cord or metal water pipe.
 Installation - connection

Waste sensor connector

Waste tube connector

Power switch
Diluent tube connector Power socket

 Connect reagent tubes / sensor to the corresponding connector (color). White connector is for Diluent solution,
Red connector is for waste. Metal connector is for Waste sensor.
 Installation - connection

Lyse is installed inside of the instrument. Open the door of lyse house
Connect lyse tubing.
 Installation – connection

Peripheral devices

LAN Port:
 Computer (Data Management Software)
 LIS

USB Port:
 Barcode scanner
 Printer
 Mouse
 Key board
USB port and LAN port  USB flash disk
 Installation - Install Printer Paper

Insert a new roll Printer Paper into the compartment as shown below:
Ensure the glaze surface face to printer (Thermal printer, 57mm width Printer paper)

Printer
 Installation – Power on

Turn on the power switch on back of the instrument.

 Installation – Starting up

Login software
User: admin
Password: admin
 Installation – Starting up

Software initialization : Then the analyzer will boot up and have a hardware self-check, fluidics initialization,
fluidics cleaning. If it passes the checking, initialization and cleaning, it will do a background test
automatically.
 Installation – Date, Time setting

Enter Management => Setup => General => Time interface to set up the correct date, time and time format.
 Installation – Starting up

Loading Reagent

Before delivery, the hydraulic system is empty, so for the first installation, instrument will give reagent empty
alarm. Please enter Reagent => Replace Reagent interface to load corresponding barcode. After apply the barcode,
analyzer will run priming to make the hydraulic system full of reagent.
 Installation – Starting up

Reagent loading

When perform loading the reagent, need input the Lyse/Diluent barcode via barcode scanner or input manually.
And the barcode of Lyse local on the bottle of Lyse, the barcode of diluent stick on the cap of diluent (As figure of
above)
 Installation – Starting up

HGB gain adjustment:

If you get HGB alarm, please adjust HGB (Gain) value as following steps.
Enter Management => Setup => System => Gain interface.
Click + or – button to adjust HGB gain value or click 4.5v button to adjust HGB gain value to get HGB blank
value around 4.5±0.1V automatically. Then click OK to save HGB gain.
 Installation – Power on

Do one more background test. Make sure blank test results are within range.
 Installation – Calibration

Enter Management => Calibration =>Calibrator Cal. interface. Input calibrator information
and target value, then test at least 5 times to finish calibration.
 Installation – Quality Control

Enter QC => Setting => New interface. Input QC information, target and limits values,
then test QC accordingly.
 Installation – Sample test

Sample Mode Edit patient information

Enter Analysis menu. Select sample mode, edit patient information, then can test
sample accordingly.
 Installation - Power off

When power off the instrument, please click Shutdown button, then the analyzer will ask for probe cleanser soak.
Finish probe cleanser soak, then instrument indicates to power off the analyzer.
 Installation – Transportation & Storage

If the instrument will be kept for a long time such as more than 3 days, it is necessary to enter Management =>
Service => [Drain All] to operate packing procedure.
After click [Drain All] button, follow instrument indication to do the flushing & draining performance. Then fix
sampling system via fixing belt and cover counting chamber like original condition via shielding paper . Finish all above
operation, you can pack the instrument for storage or sending to other place.
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Maintenance

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 Maintenance

Routine Maintenance:
Wipe the appearance of the instrument with neutral detergent, distilled water
or alcohol.
Caution: Avoid using corrosive acids, alkaline, and volatile organic solvent. Only
neutral detergent can be used.
Caution: Avoid wiping the inner of the instrument.

Daily Maintenance:
1. When the instrument shutdown, it will run daily shutdown probe cleanser
cleaning procedure.
2. If we need keep the instrument power on more than 24H, we can set daily
auto cleanser soak time in Management→ Setup→ System → Maintenance
interface. After the first 24H, when system time reach the time we have set,
the instrument will remind user to do cleanser soak.
3. Use soft cotton to clean sample probe with alcohol .
 Maintenance

Weekly Maintenance:

This instrument has weekly maintenance procedure as below steps:

A. Prepare the probe cleanser.
Operation procedure:
Management→ Service → Maintenance →(Probe Cleanser
Soak)Whole Device. Finish all the operations according to the prompts
on the screen.
 Maintenance

Weekly Maintenance:

B. Run Unclog for WBC/RBC Chamber:

Management→ Service → Maintenance →Aperture →Unclog
Run Cleaning for Fluidics:
Management→ Service → Maintenance → Fluidics Cleaning

Note: Hematology Analyzer is a kind of precise instrument, so it is

necessary to do maintenance every week. If you have a big amount of
samples, you should increase the maintenance times appropriately.
 Maintenance

Monthly Maintenance:

It is necessary to clean up dust and crystal for the instrument monthly.

Operate as follows:
a ） Power off the instrument, then pull out the power cord. Open the
right door, clean sample probe wash set and shielding box carefully (avoid
dusty fall into WBC/RBC chamber).
b) Remove the shielding box, place a shielding cloth above (WBC/RBC)
counting chamber to keep counting chamber free from dust or crystal
during maintenance. Then clean up dust or crystal on sample probe,
titanium pipe (above WBC chamber) by soft cotton, and also the
appearance of WBC/RBC chamber.
 Maintenance

Monthly Maintenance:

c) After above steps, do not run sample test immediately. we

should run operation as below firstly:

Run Unclog for WBC/RBC Chamber:

Management→ Service → Maintenance →Aperture →Unclog
Run Cleaning for Fluidics:
Management→ Service → Maintenance → Fluidics Cleaning
We do these operation in order to ensure the interior of the
pipeline be cleared.
 Maintenance

Yearly Inspection & Maintenance:

Enter into Management => Service => Self

checking interface:
1. Check Vertical, Horizontal and
Syringe motor function: if any error,
check motor, motor sensor or motor
driver board.
2. Check from Valve1 to Valve 9: When
click valve, if did not hear valve
sound, please check valve power
cable connection and valve voltage
(12V DC when click the button), or
disassemble it and clean valve, or
check motor driver board.
 Maintenance

Yearly Inspection & Maintenance:

3. WBC and RBC Aperture voltage

checking: if not in range, need to
maintenance chamber, aperture or
replace Aperture O ring.
4. Vacuum: If Vacuum pressure is not in
range, need to check vacuum system
air tightness, clean vacuum tank,
clean vacuum pump membrane
inside, check vacuum pressure sensor
function on motor driver board.
5. Air Pump: When run this function,
chambers will get bubble inside, if no
bubble, check tubing air tightness or
check air pump.
 Maintenance

Yearly Inspection & Maintenance:

1. Check and clean sample probe, incase of it is blocked inside, try dispense the diluent to
check if it is blocked.
2. Clean sample probe swab, in case of crystal affect instrument performance.
3. Clean chambers shielding box, in case of crystal affect instrument performance.
4. Check and clean WBC and RBC chambers and back bath tubing, if necessary replace
back bath tubing.
5. Replace WBC and RBC apertures’ O ring, if performance is not good even finish step 4.
6. Check Vacuum Pressure, clean Vacuum Tank and Vacuum Pump membrane inside.
7. Check Valve function, if necessary, disassemble it and clean valve inside.
8. Check tubing system, if any tube loose or dirty, maintain or replace them.
 Maintenance

Why maintenance is important

The following is an example about problem distribution of hematology analyzer :

Large part of problems is caused by no executing maintenance as requirement.

So, execute the maintenance as requirement can reduce problem rate.
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Mechanical structure

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 Mechanical structure

Touch screen: control & communicate to

instrument.
Sample probe: aspirate & dispense sample
Touch screen or probe cleanser, dispense diluent.
Start key: start sample test, dispense
diluent or start probe clean, maintenance,

Sample probe exit standby mode.

Power indicator:
Start key [ Aspirate]
Blue color: normal
Power indicator
Yellow color: standby
Red color: alarm
 Mechanical structure

USB port: connects to key board, mouse, printer,

barcode reader, USB flash disk.
LAN port: connects to PC or LIS.
Waste sensor connector: connects to waste sensor
Waste tube connector: connects to waste container
Diluent tube connector: connects to diluent box.

4 USB ports

LAN port
Power socket & switch
Waste sensor connector

Waste tube connector

Diluent tube connector

 Mechanical structure

Built-in thermal printer

Lyse house

Built-in thermal printer: print report and histogram, QC

Lyse house: Store lyse

 Mechanical structure

Sampling system

Syringe

Wash set

Sampling system: aspirate and dispense

sample, probe cleanser, diluent.
Syringe: aspirate and dispense sample,
diluent, lyse and probe cleanser.
Wash set: wash sample probe exterior
 Mechanical structure
Air pump
Reagent detector

Sampling system

Vacuum tank
Valves

WBC, RBC chambers, HGB Assembly

Liquid pump
• Air pump: mixing sample ,
• Reagent detector: detect reagent is enough or
not (bubble inside tubing or not)
• Vacuum tank : maintain vacuum pressure
• Valves: control hydraulic system
• WBC, RBC chambers: blood cell counting
• HGB Assembly: HGB measurement
• Liquid pump: drain waste liquid, generate
vacuum pressure
 Mechanical structure

Sampling system
Motor Driver board

Lyse house
Core board
Main control board

Motor Driver board: control valves, motors, pumps, sensors and

so on
Core board: CPU control and memory
Main control board: signal receive and control (communication)
07
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Hardware and Hydraulic

System

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 Hardware-Main control board

J16 J18 J22 J17 J20 J23

J16: power input 12V, 5V DC
J4: power input 110V DC for aperture shocking
J21 J1: power input ±12V DC
J15: LAN port
P4
J26: USB ports
J12 J25: USB ports
J18: Communication port (connect to motor driver
J27 board )
J25 J22: Reserved
J17: Reserved
J14 J20: Reserved
J26
J23: Printer port
J15 J5 J21: LCD data cable port
P4: LCD backlight port
J7 J12: Touch panel port, Micro switch and Indicator
J1
J27: Reserved
J4 J14: HGB signal port
J5: RBC signal port
J7: WBC signal port
 Hardware-Driver board

J12 connector, from top to bottom:

1&2: Micro switch

3 - 8: Indicator

9 - 14: Touch panel

 Hardware-Motor Driver board

Air pressure sensor

J7 J8 J15 J5 J13 J14 JM1 J9: Power input 24V, 12V, 5V DC

J7: Sensors ports
J8 ： Ambient Temperature sensor port
JM2
J15: Null
J5: Null
J13: Valve control ports
J14: Liquid pump & air pump control port
JM1: Sampling module V & H motor port
JM2: Syringe motor port
J4: Communication port (connect to main
J9 J4 control board)
 Hardware-Driver board

J7 connector, from left to right side:

1: Null
1 17
2 & 4: Diluent sensor board port
3: Null
5 - 6: Lyse sensor board port
7: Waste sensor connector
8 -9: Null
10 - 11: Syringe motor sensor port
12-13: Sampling module V motor sensor port
14 -15: Sampling module H code sensor port
16 - 17: Sampling module H home sensor port
 Hardware-Motor Driver board

J13 connector, from left to right side:

1: Null
2 - 8: Null
9: SV2
1 17
10: SV4
11: SV6
12: SV8
13: SV7
14: SV5
15: SV3
16: SV1
17: SV9
 Hardware-Motor Driver board

J14 connector, from left to right side:

1. Liquid pump port.
2. Air pump control port.
3 - 6. Null
1 2 3 4 5 6
JM1 connector, from left to right side:
1 - 4: Sampling module V motor port
5 - 8: Sampling module H motor port
1 8
JM2 connector, from left to right side:
1 - 4: Syringe motor port
5 – 8: Null
1 8
 Hardware

Reagent sensor board Power indicator board

（ detector ）
Hydraulic system – Hydraulic diagram
 Hydraulic system– solenoid valve (SV)

Aspirate/dispense diluent/lyse, connects to reagent syringe&SV6&DIL

SV01
detector
SV02 Connects to sample syringe, sample probe and SV5
Connects to sample probe wash set (out) and connect to Vacuum tank
SV03
(port 1)
Connects WBC back bath (together with RBC back bath) and connect
SV04
to Vacuum tank (port 3)

SV05 Connects to RBC back bath, sample probe wash set (in) and Valve 2

SV06 connects to sample syringe and SV7, SV1

Aspirate/dispense lyse, dispense diluent (connect to Lyse detector,
SV07
WBC bath, SV6)
Mixing RBC chamber, drain out liquid ( connect to Vacuum tank, air
SV08
pump, buffer bath Ⅱ)
Mixing WBC chamber, drain out liquid (connect to Vacuum tank ， air
SV09
pump, buffer bath Ⅰ)
 Hydraulic system–solenoid valve (SV)

2-way solenoid valve: 3-way solenoid valve

a. In and Out port will connect when • NC (Port 1): normal closed (connect to COM if the valve is energized)
the valve is energized
b. In and Out port will disconnect • NO (Port 3): normal open (connect to COM if the valve is de-energized)
when the valve is de-energized • COM (Port 2): common port
 Hydraulic system– syringe

Name Specification Function

Small syringe 250ul Aspirate sample
(sample)

Big syringe 10ml Aspirate and dispense diluent and lyse, sample, probe cleanser.
(diluent/lyse) probe wash set cleaning and back flush.
 Hydraulic system– liquid pump

Function:
Generate vacuum pressure for vacuum tank, support sample probe wash
set(sample probe cleaning), WBC/RBC chamber washing, blood cell counting.
 Hydraulic system– air pump

Function:
Mixing WBC & RBC chamber and assist chamber washing.
 Hydraulic system– sample probe

Function:
Aspirate & dispense sample or probe cleanser, dispense diluent.
 Hydraulic system– sample probe wash set

Function:
Wash sample probe exterior.
 Hydraulic system–vacuum pressure sensor

Function:
Monitor vacuum tank pressure. Give alarm if vacuum pressure is out of range.
Test Flow chart
Test Flow chart
 Hydraulic system–time sequence

1. Aspirate sample, drain WBC chamber.

2. Sample probe raise up and wash sample probe exterior.
3. Drain RBC chamber. Add diluent into WBC chamber, then add sample with diluent into WBC chamber.
4. Mix WBC chamber, sample probe raise up and wash sample probe exterior.
5. Add diluent into RBC chamber, sample probe raise up, mix WBC and RBC chamber.
6. Sample probe aspirates diluted sample from WBC chamber, then raise up and wash sample probe exterior.
7. Add lyse and diluent into WBC chamber, mix WBC and RBC chamber.
8. Add diluted sample into RBC chamber, mix WBC and RBC chamber. Sample probe raise up and wash probe
exterior.
9. Counting blood cell.
10. Drain WBC chamber, drain RBC chamber.
11. Add diluent into WBC chamber and mix WBC chamber, add diluent into RBC chamber.
12. Darin WBC chamber, sample probe raise up. Add diluent into WBC chamber, mix WBC and RBC chamber.
13. Sample probe goes to sample test position for next sample testing.
1. Aspirate sample and diluent, drain WBC chamber
2. Sample probe raise up and wash sample probe exterior
3.Drain RBC chamber. Add diluent into WBC chamber, then add sample
with diluent into WBC chamber.
4.Mix WBC chamber, sample probe raise up and wash sample probe
exterior.
5. Add diluent into RBC chamber, sample probe raise up, mix WBC and RBC chamber.
6. Sample probe aspirates diluted sample from WBC chamber, then raise up
and wash sample probe exterior.
7. Add lyse and diluent into WBC chamber, mix WBC and RBC chamber.
8. Add diluted sample into RBC chamber, mix WBC and RBC chamber.
Sample probe raise up and wash probe exterior.
9. Aspirate the RBC and WBC sample solution to the back bath at
the same time, start counting
10. Drain both chamber
11. Add diluent into WBC chamber and mix WBC chamber, add diluent
into RBC chamber.
12.Darin WBC chamber, sample probe raise up. Add diluent into WBC chamber, mix WBC
and RBC chamber.
 Hydraulic system–Time sequence

• Probe cleanser Maintenance (shut down

instrument):

• Big syringe aspirate probe cleanser, then add

into WBC, RBC chamber.

• Vacuum pump aspirate some Maintenance liquid to

WBC, RBC back bath for soaking.

• Drain and wash chambers after Maintenance.

08
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Trouble Shooting

www.zybio.com
 Trouble Shooting-Aperture clog

How does the analyzer report clog error?

During blood cell counting procedure, blood cells pass through
aperture and the analyzer count blood cells signal. This process
spent about 10 seconds. The analyzer divides this process into 8
parts. The analyzer will get RBC WBC PLT result for each part
then compare the results between these 8 parts. If it is clogged
during counting, there will have a big difference between some
parts. In this way the analyzer can judge whether the aperture is
clogged or not. Also we call it fixing time method.
 Trouble Shooting-Aperture clog

How does clog error appear?

If we test sample with the analyzer, but don’t do maintenance very
well, some protein or lipid will adhere on the aperture or tubes.
After we use it for a long time, these protein and lipid will
accumulate and finally cause the aperture clogged.

How to remove clog error?

Use unclog function to remove clog with high voltage shock and
back flush. If it doesn’t work, do Whole Device Cleanser Soak
operation with probe cleanser. If it still doesn’t work, we need to
disassemble the counting chamber to clean WBC/RBC aperture
manually.
 Blank result abnormal

Blank result abnormal:

1. Check whether power supply is stable and whether power grounding is good?
If power is stable and power grounding is good, go for next step.
2. Temperature is high also can make PLT high result (Operation temperature:
15-35 ℃).
3. Check whether reagents are expired or contaminated. If yes, replace it.
4. Try to solve the problem by unclog, cleaning, whole device soak
function(Tubing dirty, lipid/protein may adhere on aperture.).
5. Check whether back bath tube is dirty or not. If it is dirty, clean it or replace it.
6. If there are any dust or crystal adhere to sample probe, sample probe wash
set, shielding box or counting chamber, clean it with alcohol manually.
 Reagent issue

Reagent alarm ：
A. Reagent empty
1. Check whether the corresponding reagent bottle is empty.
2. If yes, replace new reagent and load the barcode.
3. If reagent is enough and alarm still exist, enter Management => Status => Volt./Current
interface to check the reagent sensor voltage(0~2.5V means empty, 3.5V~5V means full.).
4. Enter Management => Service => Prime interface, run prime function to avoid bubble in tube.
B. Reagent expired
1. Enter Management => Setup => Reagent menu.
2. Select “One key elimination” to activate clear reagent expired alarm function. Dis-select
“Reagent expired” option and click OK to save.
3. Then we can click the alarm area, on the pop-up dialog box have one key clear alarm function.
Click clear to cancel the alarm and close the dialog box, then we can continue use the reagent.
C. Reagent run out
Reagent use up, need to load a new reagent.
 HGB blank abnormal

HGB adjustment:
1. Enter Management=> Setup => System=> Gain.
2. Change HGB gain value to get HGB blank value around
4.5±0.1V, then save HGB gain.
 Counting chamber overflow issue

WBC chamber overflow :

(Ps: Use hydraulic diagram to check it)

1. WBC bottom blocked (crystal or some particles )

2. Buffer bath blocked (strainer dirty)
3. Valve 9 blocked or defective
4. liquid pump defective
5. The tube is squeezed

RBC chamber overflow :

1. RBC bottom blocked (crystal or some particles )

2. Buffer bath blocked (strainer dirty)
3. Valve 8 blocked or defective
4. liquid pump defective
5. The tube is squeezed
 Trouble Shooting-Result is abnormal

Exchange RBC(J5) and WBC(J7) signal

Only one chamber’s counting result is 0: cable on main control board (Need
to disconnect J4 cable).
WBC Chamber: WBC&HGB RBC Chamber: RBC&PLT After exchange, here will have four
kinds of conditions.
For example: WBC chamber’s counting result is 0, and RBC
chamber’s counting result within range

WBC RBC Conclusion

1 Value Value Reconnect corresponding cable

2 Value 0 WBC chamber issue

J7 J5
3 0 Value WBC signal channel defective

4 0 0 Both WBC chamber and WBC signal channel

defective
THANK YOU

Zybio Inc., Floor 1 to Floor 4, Building 30, No.6 of Taikang Road,

Block C of Jianqiao Industrial Park, Dadukou District, Chongqing, China 400082
Tel: +86 (0)23 6865 5509 Fax: +86 (0)23 6869 9779
Email: info@zybio.com Website: www.zybio.com