Professional Documents
Culture Documents
INTRODUCTION TO NEUROSCIENCE
Wednesday lab
Howard, Clare E. Gladstone, Alexandra
Kativhu, Chido L. Glick, Michelle S.
Chapter 15 (490-497)
Tuesday lab
Kim, Daniel W. Hartley, Iris R.
LaMontagne, Laurel Holaday, Eric J.
Mattison, Jamie E. Jeppson, Pamela
Montgomery, Tracy Jiang, Debbie C.
Navarro, Yasmin Kundu, Surya
Rivera, Ashley M. Lim, Tiffany M.
Salgado, Sanjay M. Ludwig, Susannah W.
Thein, Thuzar Moin, Emily E.
Tsareva, Alina O'Loughlin, Kerry
Zhang, Wen Romanowicz, Jennifer
Zhu, Victor Waskom, Michael L
Neuroscience definition & history –
• Neuroscience: The
Chapter
study of1 behavior and the
mind through the study of the nervous system.
100 m
2. Golgi method Dendrites
1 m
Cell membrane
Neurons: a composite picture
Inject HRP
into left eye
Glass micropipette:
- Tip diameter 0.5 um
- Filled with salt solution
- Records electrical
difference across the
cell membrane
Fig. 3.11
Single neuron recording: extracellular
Sharpened metal probe, tip diameter 1 – 5 um
Records electrical currents flowing outside neurons
Voltage
Time
E. Studying chemical signalling:
Pharmacology
• How can chemicals have effects in the body
at very low concentrations?
• Concepts:
– Receptors
– Specific binding
– Multiple subtypes for a single neurotransmitter
– Agonists, antagonists
Measuring chemicals and chemical signals
(1) Immunological methods
– Antibody to specific protein, e.g.
receptor
– Single brain section:
Immunohistochemistry
– Piece of brain tissue: Western
blotting – ‘gel’
Example 2: lesions
• Cut, freeze, heat (via high electrical frequency), toxic
chemical (as in our lab later this semester)
• Problems:
• Not always precisely specific to desired region
• Fibers of passage
Example 3: Brain injections of
neurochemicals
• Neuropeptide-Y (NPY) and Melanin Concentrating
Hormone (MCH) both increase food intake.
• NPY neurons project to MCH neurons.
– Hypothesis: MCH neurons mediate the downstream effects
or “commands” of NPY on feeding.