Hemax 530 training

Catalog

Installation

Sample test

QC test

Manual calibration

Fluidic diagram &fluidic sequence

Parts introduce &check mechanic

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 Installation

• Requirement
• Connected dataline /power supply /reagent tu
bes
• Remove sampling controller
• Install software and connect computer

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 Sample test
• Open mode
• Auto mode
• Worklist mode

SOP

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QC test

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 Manual calibration
• Manual calibration procedure is as follows:
• 1. In Sample Test menu, use calibration sample to test several times (at
least three).
• 2. Record the tested data.
• 3. Calculate new calibration coefficient
• Calculate new calibration coefficient as per formula below

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Fluidic diagram

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 Parts introduce&check mechanic
1.sheath flow syringe
2.sample probe assembly
3.mix assembly
4.sample transfer unit
5.Diff reaction bath assembly
6.WBC chamber
7.RBC chamber
8.Negative pressure chamber
9.Positive pressure chamber
10.
Negative pressure pump
11.
Positive pressure pump
12.
Optical assembly
13.
Auto loader panel unit
14.
Ul(sample ) syringe assembly back
15.
Lys syringe assembly
16.
Diluent syringe assembly
17.
Liquid-level detect assembly
18.
Driver panel assembly
19.
Main control panel assembly
 Requirement
• Power: AC100V~240V, Input Power ≤250VA, 50/60HZ.
Note:(1)AC power must be well earthed.
(2) AC power shall be stable,we advice customer equip UPS.

• (External) Computer:
CPU: >1.4G
RAM: >2G
Hard disk space available: >20G
Graphics Card: OpenGL 2.0 or above
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Display aspect ratio: 10: 6
Connected dataline /power supply /reagent tubes

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Remove sampling controller

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 Install software and connect computer
• Step 1, install software.
• Step 2, change IP address of the PC to 10.0.0.102
• Step 3, open the software and then power on the instrument, after 10 seconds
if the light which is located on the up right side of the screen become green
that means the PC connected with instrument well.
• Step4, prime all reagent 1 or 2 times.

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sheath flow syringe

• aspirate the incubated sample from the

Diff bath
• push the sheath fluid into the flow
chamber
• clean the sample preparation tubing
and flow chamber
• Clean diff bath

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 sample probe assembly
• consist of sampling needle and swab
(sampling needle cleaning head )
• Swab’s function :clean the outer wall of
the sampling needle .
• How to clean the swab :
After cleaning and reassembling the swab
(and ensuring that the inflow/outflow
interface tubing is connected correctly ),turn
on the device and to to “service ----clean
“,double click the “clean the sample probe
“button to perform the operation .

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mix assembly

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sample transfer unit

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Diff reaction bath assembly
• Provide diff1&2 to
optical assembly
• Incubate diff1&2
• How to PM diff bath
Clean diff bath with cotton
swab .
turn on the device and to to
“service ----clean “,double click
the “clean the DIFF bath
“button to perform the
operation .

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 WBC chamber

• Impedance method is used during WBC

measurement
• The blood sample and HGB lyse are mix well
for a complete reaction then HGB can be
measured by colorimetry.
• Provide diluent sample to RBC

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 RBC chamber

Impedance method is used during RBC

&PLT measurement

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Negative pressure
chamber
• Through the negative pressure
chamber ,WBC/RBC/PLT travel
through the aperture of the
chamber for being counted .
• any waste that counting and from
cleaning the back chamber of the
WBC and RBC flows into the
negative-pressure chamber.
• Provide power for cleaning both
rear chambers.

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Positive pressure
chamber
• Generate air bubbles in the
DIFF /WBC/RBC
• Release negative chamber
pressure

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Negative pressure pump

• PP1:drain WBC/Swab
• PP2:drain DIFF /RBC/negative
chamber
• establish a negative pressure of -
30Kpa for negative chamber

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Positive pressure
pump

Provide power with positive chamber

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Optical assembly

Classify WBC to 5 parts

Auto loader panel unit

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Ul(sample ) syringe assembly
• Push the sample which be mixed in DIFF to
flow chamber （ 250ul ）
• Aspirate the sample from sample tube/WBC
chamber (100ul)
• Push diluted-sample to RBC chamber (100ul)
• Built a bridge for Cleaning inside &outside of
the probe (100ul)

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Lys syringe assembly

• Diff1&2 be provided with

DIFF bath
• HGB be provide with WBC
chamber
Diluent syringe assembly

• Clean both Chambers

&probe
• Provide diluent with
both counting
Chambers

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Liquid-level detect assembly

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Driver panel assembly

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Main control panel assembly

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