Lesson 8: Topic 3

Recombinant DNA
and
Cloning Techniques
Dizon, Diesebel A.
BSED 3D
LEARNING OUTCOMES
At the end of the lesson, you should be able to:
• Discuss recombinant DNA and cloning techniques
 Genetic engineering
• Use of technology to change the genetic makeup of an organism
• recombinant DNA (Rdna)
• National Human Genome Research Institute (NHGRI).
• Genentech, 1976.
 Recombinant DNA technology
• 1970s
• Food and Drug Administration (FDA)
• Human insulin, in 1982
Genetically Modified Organisms (GMOs)
• is an animal, plant, or microbe DNA has been altered using genetic
engineering techniques.
 DNA cloning
• starting point for many genetic engineering approaches to biotechnology
research.
HOW IS DNA CLONED IN CELLS?
 The four main steps in DNA cloning are:
• Step 1. The chosen piece of DNA is ‘cut’ from the source organism using restriction
enzymes.
• Step 2. The piece of DNA is ‘pasted’ into a vector and the ends of the DNA are joined with
the vector DNA by ligation.
• Step 3. The vector is introduced into a host cell, often a bacterium or yeast, by a process
called transformation. The host cells copy the vector DNA along with their own DNA,
creating multiple copies of the inserted DNA.
• Step 4. The vector DNA Is isolated (or separated) from the host cells’ DNA and purified.
Thank you!