Biochem Laboratary Equipments

BIOCHEMISTRY (LABORATORY)
Activity #1
Laborator
y
Apparatus
Laboratory Apparatus
refers to the various tools or vessels
used by scientists working in a laboratory
in order to complete laboratory
experiments
they are used in different analysis and
synthesis of different chemicals and
analytes
Laboratory Apparatus
It allows for safe handling of chemical
substances specially those that are
hazardous in nature
The Lab Apparatuses
A. Glasswares
1. Beaker
Description:
They are cylindrical, have a flat
bottom, and a small spout on
the top to pour chemicals
Composition:
Made up of Borosilicate Glass
or plastic
The Lab Apparatuses
A. Glasswares
1. Beaker
Function:
They are often used to hold,
mix, and heat individual
chemicals or chemical
mixtures.
The Lab Apparatuses
A. Glasswares
2. Erlenmeyer Flask
Description
conical body, flat bottom,
and a cylindrical neck
The Lab Apparatuses
A. Glasswares
2. Erlenmeyer Flask
Description
conical body, flat bottom,
and a cylindrical neck
The Lab Apparatuses
A. Glasswares
2. Erlenmeyer Flask
Function:
It is one of the most commonly
used flasks to carry out various
experiments in the chemistry lab,
such as titration, filtration,
crystallization, etc
The Lab Apparatuses
A. Glasswares
2. Erlenmeyer Flask
Function:
It is used to hold different
layers of reactions, heat
solutions, and grow liquid
cultures of bacteria. It can
also be used for heating and
boiling purposes.
The Lab Apparatuses
A. Glasswares
2. Erlenmeyer Flask
Functions:
Cylindrical neck of a conical
flask can accommodate a
glass stopper, and it also
provides support to fit
funnels.
The Lab Apparatuses
A. Glasswares
3. Volumetric Flask
Description
It is composed of a flat bottom
conical-shaped bulb attached to
an elongated neck with an
engraved ring that serves as a
marking that indicates a specific
volume
The Lab Apparatuses
A. Glasswares
3. Volumetric Flask
Function
A volumetric flask is used to measure very
precisely one specific volume of liquid
(100 mL, 250 mL, etc., depending on which
flask you use). This flask is used to
accurately prepare a solution of known
concentration.
The Lab Apparatuses
A. Glasswares
4. Graduated Cylinder
Also known as volumetric cylinder
or measuring cylinder
Description:
It has a narrow cylindrical shape. Each
marked line of the graduating cylinder
represents the amount of solution
measured.
The Lab Apparatuses
A. Glasswares
Desctiption
Graduated cylinders contain a strong
base or foot for stability and a spout to
enable pouring out the contents.
Some glass cylinders contain a plastic
bumper or ring to help prevent
inadvertent breaking if the cylinder
falls over due to its unsteadiness.
The Lab Apparatuses
A. Glasswares
Composition
It is typically made of polypropylene
because of its excellent chemical
resistance or polymethylpentene
because of its transparency
The Lab Apparatuses
A. Glasswares
Function
It is used to measure the volume of
liquids in the chemistry lab
The Lab Apparatuses
A. Glasswares
5. Funnel
Description
- It is a cone-shaped device
Function
used to pour liquids from one
vessel to another without the
risk of spillage.
The Lab Apparatuses
A. Glasswares
5. Funnel
Function
They are usually made of glass and
plastic and comes in a variety of
sizes that can be used for any
process that requires pouring
chemicals solutions in flasks, test
tubes, beakers, and other vessels.
The Lab Apparatuses
A. Glasswares
5. Funnel
Function
They can also be used
with filter paper to filter
out impurities from the
solution.
The Lab Apparatuses
A. Glasswares
6. Stirring Rod
Description
They are usually made of
solid glass, about the
thickness and slightly
longer than a drinking
straw, with rounded
ends.
The Lab Apparatuses
A. Glasswares
6. Stirring Rod
Function
 The primary function
of a stirring rod is to
stir solutions by hand.
 Helps in decanting
liquid from containers
The Lab Apparatuses
A. Glasswares
6. Stirring Rod
Function
 Breaking up emulsions
and inducing
crystallization.
The Lab Apparatuses
A. Glasswares
7. Pipet/Pipette
Description
It is a typically consists of
a narrow tube into which
fluid is drawn by suction
The Lab Apparatuses
A. Glasswares
7. Pipet/Pipette
Function
 used to transport
specific amounts of
fluids from one place
to another.
The Lab Apparatuses
A. Glasswares
8. Burette
Description
It is a long cylindrical
tube with a tap at the
bottom, and it is usually
mounted on a stand with
the help of the clamps
The Lab Apparatuses
A. Glasswares
8. Burette
Description
It has a volume scale
engraved along its side
which provides the
volume readings for
normality equations.
The Lab Apparatuses
A. Glasswares
8. Burette
Function
It is widely used to
provide a controlled flow
of reagents during their
volumetric analysis or
titration
The Lab Apparatuses
A. Glasswares
9. Condenser Leibig
Description
The condensing column is made
up of a straight glass tube,
through which the gas flows,
surrounded by a water jacket
that aids in the cooling of the
gas.
The Lab Apparatuses
A. Glasswares
9. Condenser Leibig
Description
a glass tube within
another glass tube
The Lab Apparatuses
A. Glasswares
9. Condenser Leibig
Function:
 It is used to cool and condense a
gas to a liquid, which is commonly
done as part of the chemical
distillation process.
The Lab Apparatuses
A. Glasswares
11. Evaporating Dish
Description
It is a bowl-like apparatus that
resembles a crucible
Functions
 It is used to evaporate liquid
substances
The Lab Apparatuses
A. Glasswares
12. Crucible Dish and Cover
Function
 It is used to heat chemical
compounds with a bunsen
burner to very high
temperatures
The Lab Apparatuses
A. Glasswares
12. Crucible Dish and Cover
Description
 a ceramic or porcelain
container capable of
withstanding extreme
temperatures, whilst the
cover is designed to prevent
heat escaping from the
crucible itself
The Lab Apparatuses
A. Glasswares
13. Thermometer
Description
The laboratory thermometer can
be easily identified through its long
stem with a silver bulb at its
bottom.
The Lab Apparatuses
A. Glasswares
Thermometer
Function
These are devices used to measure
temperature
The Lab Apparatuses
B. Other Lab Apparatuses
Spatula
Description
These are implements with
flattened ends which are most
commonly made of stainless
steel
The Lab Apparatuses
Spatula
Function
It is a tool used to carry, mix,
or spread solid or paste
materials in the chemistry lab.
The Lab Apparatuses
Medicine Dropper
Description:
Consists of a tube of glass or
plastic with a flexible rubber
handle
Function
To dispense small amounts of
medicine
The Lab Apparatuses
Test Tube Rack
Description:
It is composed of one
perforated sheet arranged over
a plain sheet of plastic or metal
Function
It holds the test tubes in an
upright position
The Lab Apparatuses
Test Tube Brush
Description:
They consist of twisted wire
handles connected to bristles
that are made of Nylon or a
similar material such as Tynex
The Lab Apparatuses
Test Tube Brush
Function
It is used to clean the test
tubes used in the experiment
https://www.reference.com/science/function-test-tube-
brush-bf3b6d9f2676a09a
The Lab Apparatuses
Crucible Tong
Description
They are shaped like a pair of scissors,
the only difference is that a regular pair
of scissors have sharp blades, while
crucible tongs have a pair of metal blades
that are blunt. The blades of the crucible
tongs are curved to facilitate proper grip
on the object
The Lab Apparatuses
Crucible Tong
Function
They are mainly used to hold hot
equipment like crucibles, flasks, beakers,
etc.
https://studiousguy.com/crucible-tongs/
The Lab Apparatuses
Crucible Tong
Function
They are mainly used to hold hot
equipment like crucibles, flasks, beakers,
etc.
https://studiousguy.com/crucible-tongs/
The Lab Apparatuses
Clamp
Description
It typically consist of a shaft and
bosshead which is secured with hand-
tightened thumbscrews, knurled nuts
or wingnuts onto a supporting rod,
stand or scaffold, and a pair of two or
three-pronged, adjustable closing
jaws
https://www.fishersci.co.uk/gb/en/products/I9C8K6PU/clamps.html#:~:text=A
%20laboratory%20clamp%20is%20an,columns%2C%20funnels%20and
%20overhead%20stirrers.
The Lab Apparatuses
Clamp
Function
They are used to affix glass
lab ware to a rod lab rack
matrix.
The Lab Apparatuses
Iron Ring
Description
It comprises a conjoined metal ring and
radially-extending rod. In some cases, the
rod terminates in a screw clamp for
attachment to a retort stand or other
support; in others, the rod may be attached
to a stand by means of a laboratory clamp
holder
The Lab Apparatuses
Iron Ring
Function
It is commonly used in chemistry
laboratories for supporting apparatus
above the work surface
The Lab Apparatuses
Iron Stand and Base
Description
It consists of a heavy stamped steel base
on which a steel rod is vertically erected.
Various holding equipment such as rings
and clamps are screwed to the steel rod
at desirable heights to hold the target
equipment.
The Lab Apparatuses
Iron Stand and Base
Function
It is a piece of supporting
equipment designed to hold
other laboratory apparatus
such as beakers, flasks,
burets, etc
The Lab Apparatuses
Tripod
Description
It is a three-legged
platform. It is made of
light metals such as
stainless steel or
aluminum and is
designed to be light
and easy to carry.
The Lab Apparatuses
Tripod
Function
It is used to support
vessels while they are
being heated on a
bunsen burner
The Lab Apparatuses
Bunsen Burner
Description
A Bunsen burner is a
type of gas burner
designed to provide a
single flame with the
desired intensity.
The Lab Apparatuses
Bunsen Burner
Description
It is used to heat substances.
It is also used to sterilize
small objects, heat
chemicals, burn broken
glass, and for many other
purposes
The Lab Apparatuses
Wire Gauze
Description
It is a metal wire interwoven
sheet that sits between the
beaker and the tripod stand
The Lab Apparatuses
Wire Gauze
Function
a wire mesh is utilized to
diffuse the heat from the
flames since glassware
cannot be heated directly
The Lab Apparatuses
Clay Triangle
Description
A triangular device made of
heat-resistent clay
https://sciencing.com/function-clay-triangle-8578080.html
The Lab Apparatuses
Clay Triangle
Function
It is used in conjunction with
other lab equipment to create
a stable framework in which to
place a substance -- usually a
solid chemical -- while it is
heated to a high temperature.
The Lab Apparatuses
Aspirator
Description
It is an apparatus for
producing suction or moving
or collecting materials by
suction
The Lab Apparatuses
Aspirator
Function
It moves air or liquid
through suction
The Lab Apparatuses
Mortar and Pestle
Description
These are ancient tools
used to crush and grind
ingredients or
substances into a thin
paste or powder
The Lab Apparatuses
Mortar and Pestle
Function
They are used in
chemistry labs to grind
chemical crystals or
tablets into powder or
paste form.
The Lab Apparatuses
Thermometer
Function
These are devices used to measure
temperature
The Lab Apparatuses
Top Loading Balance
Description
It is a balance with an open
weighing pan loaded directly
from the top. It has no draft
shield.
The Lab Apparatuses
Top Loading Balance
Function
They usually can measure
objects weighing around
150–5000 g
The Lab Apparatuses
Triple Beam Balance
Description
The triple beam balance is a measuring instrument
comprising a beam supported on a fulcrum. On one side
of this beam, is a pan on which the object to be measured
is placed, while on the other side, the beam is split into
three parallel beams, each carrying a known weight and
together culminating into a pointer pointing to a fixed
scale.
The Lab Apparatuses
Triple Beam Balance
Function
The triple beam
balance is a tool for
precisely measuring
mass

The Lab Apparatuses
Hot Plate
Description
It has a flat surface
with heating elements

The Lab Apparatuses
Hot Plate
Function
Hot plates are frequently
used in the laboratory to
perform chemical
reactions and to heat
samples.
The Lab Apparatuses
Hot Plate
Function
Hot plates are frequently
used in the laboratory to
perform chemical
reactions and to heat
samples.
The Lab Apparatuses
Centrifuge
Description
• It consists of a head or rotor,
carriers, or shields that are
attached to the vertical shaft
of a motor or air compressor
and enclosed in a metal
covering. It always has a lid.
The Lab Apparatuses
Centrifuge
Function
• It is used to separate mixtures
based on mass and density of
the component parts.

1. The different apparatuses used when the
procedure involves heating of chemicals using a
Bunsen burner?
(According to different possible setups)

Setup 1
1. Clay Triangle
2. Iron Stand and Base
3. Ring clamp
5. Glassware that will
hold the analyte. (Beaker,
Crucible+Lid, Erlenmeyer Flask)
Bunsen burner?

Setup 2
1. Clay Triangle
2. Tripod
Bunsen burner?

Setup 3
1. Wire Mesh
2. Tripod
Bunsen burner?
(Other apparatuses that can also be involved)
o Tongs
o Thermometer
o Test tube Holder
Beam Balance and the Top Loading Balance
Beam Balance Top Loading

Balance
• This type of • Less sensitive compared to
laboratory balance Analytical Balance but is
Both are greater in sensitivity
is less sensitive than tools used
a top-loading compared to Beam balance
in
balance • They can measure objects
measuring
• It could measure objects the mass with masses of 150–5000 g.
with a mass of 610 g of an They can’t measure objects
and below. object of extremely low masses like
laboratory balances
• It is more difficult to • Allow measurements to
operate be made quickly
The difference between
(Other apparatuses that can also be involved)
o Tongs
o Thermometer
o Test tube Holder
REFERENCES
• Akshit. (2021, August 17). List of chemistry laboratory apparatus and their uses.
StudiousGuy. Retrieved October 3, 2022, from https://studiousguy.com/list-of-
chemistry-laboratory-apparatus-and-their-uses/#Liebig_Condenser
• IAC Publishing. (n.d.). What is the function of a stirring rod? Reference.
Retrieved October 3, 2022, from https://www.reference.com/science/function-
stirring-rod-c8873eb5258ab47f
• Volumetric flask. Volumetric Flask: Description. (n.d.). Retrieved October 3, 2022,
from
https://www.chemedx.org/JCESoft/jcesoftSubscriber/ChemPagesLab/modules/
volflask/volflaskdesc.htm
• Editors, M. N. (2022, September 29). Triple beam balance - parts, procedure,
uses. Microbiology Note. Retrieved October 3, 2022, from
https://microbiologynote.com/triple-beam-balance/
• Hot plate use and safety in laboratory - department of chemistry. (n.d.). Retrieved
October 3, 2022, from
https://chem.wisc.edu/wp-content/uploads/sites/1130/2019/12/HotPlateSafety.pdf
REFERENCES
Laboratory spatulas information. Laboratory Spatulas Selection Guide: Types,
Features, Applications | Engineering360. (n.d.). Retrieved October 3, 2022, from
https://www.globalspec.com/learnmore/labware_scientific_instruments/
clinical_research_labware/laboratory_spatulas
Patterson, J. (2021, January 11). Uses of a medicine dropper. Healthfully. Retrieved

October 3, 2022, from https://healthfully.com/113694-uses-medicine-dropper.html
https://www.scalepeople.com/blog/four-types-of-laboratory-balances/

Biochem Laboratary Equipments

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Biochem Laboratary Equipments

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BIOCHEMISTRY (LABORATORY)

(According to different possible setups)

(According to different possible setups)

(According to different possible setups)

(Other apparatuses that can also be involved)

Beam Balance Top Loading

(Other apparatuses that can also be involved)

Patterson, J. (2021, January 11). Uses of a medicine dropper. Healthfully. Retrieved

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