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• The inclusion of HIM in the diet for 12 weeks did not worsen the laying
performance or affect the function of the liver and pancreas.
• HIM did not significantly alter most of the serum biochemical indices but
increased the serum Ca content.
• It may be regarded as an efficient alternative protein source for laying
hens.
• It negatively altered the liver histology and unfavorably changed the yolk
FA profile, which needs further investigation.