INTRODUCTION: High Hydrostatic Pressure (HHP) or High-Pressure Processing (HPP) or Ultra High Pressure processing is considered as one of the most successfully commercialized non- thermal processing technology. It offers the food industry a technology that can achieve the food safety of heat pasteurization while meeting consumer demand for fresher-tasting minimally-processed foods. It aims to process liquid and solid foods with a high content of moisture. With the help of this processing technique, microorganisms can be easily destroyed at 300-900 MPa without breaking the covalent bonds and degrading vitamins, flavor and color molecules in the process and thus the effect on processed food is minimal. Also, the positive effect consists of the recession of excessive thermal treatments and chemical preservatives. The technique is independent of the shape, size or composition of products. High pressure processing technique is widely used in the production of packaged vegetables, fruits, meat, seafood, and dairy products. Examples- jams, puree, juices, meat products, and fish products.
Why to go for Non-thermal processing?
The food industry and consumers have significant interest in nonthermal pasteurization processes because they offer better quality and nutrition retention and are more energy efficient than traditional thermal processes. Non-thermal processes may also create value-added products and open new market opportunities. One of the most valid criticisms for traditional thermal pasteurization is the likelihood of bacteria forming inside the processed food or beverages. High Pressure Processing System 1. Components Pressure vessel Pressure transmitting Fluid Material handling pressurizing System
Heating/cooling system Intensifier pump 2. Principles involved Isostatic Principle Le Chatelier’s Principle PROCESS
The HPP process begins by
loading a vessel with product in its final, flexible, packaging. The vessel is then inserted into to HPP machine where cold water is pumped into the vessel creating isostatic (even) pressure on the product and its packaging. Once the pressure reaches the desired PSI the product is held at that pressure for a few minutes, pressure and hold times vary by product. During the pressure hold time, pressure is transmitted uniformly throughout the packaged food disrupting the microbial biochemistry which preserves freshness and increases shelf life. After reaching the desired hold time, the pressure is released, the vessel is removed from the machine, and then product is dried, inspected, and any labels, extra packaging, or tagging of the product is completed. Types of HPP operating systems There are three major types of industrial high operating systems: 1.Batch system 2.Continuous system 3.Semi-continuous system The batch mode has the advantage that can process both liquid and solid products, and in this case, products are usually, pre-packed first. Whereas continuous and semi-continuous systems can be applied only to liquid products (Elamin et al., 2015). Characteristics of HPP ◦ Effect of high pressure on micro-organisms: When high pressure damages cell membrane and its structures, the absorption of nutrients in the cell of microorganisms is affected, the disposal of waste products in the cell is disrupted and the natural pathway if metabolism gets destroyed. Hence HPP can be applied to inhibit the growth of microorganisms in food (Huang et al., 2020). ◦ Non-thermal pasteurization: Traditional pasteurization involves the elimination of pathogenic microbes by high temperature to maintain the quality of products during cold chain distribution. High pressure pasteurization involves the use of water as a pressure transmission medium to transmit a pressure of 400-600 MPa to packaged food products for the elimination of pathogens. ◦ Influence on food components: During the treatment process in HPP, new aromatic components are not generated, and the original colors, flavors, quality, and nutrients of food products can be retained to a greater extent compared with traditional thermal processing technologies. However, HPP also affects microstructures and nutritional contents of different food products to varying degree ◦ Hurdle processing: To satisfy the demands of consumers for natural foods, which includes freshness, stability, safety, tastiness and affordability of food products, food industries tend to adopt a combination of hurdle technologies and various antimicrobial factors for the preservation of food products. As HPP is only performed after packaging, it can serve as the final hurdle technology in a series of bacteria inhibition technologies that reduce microbial risks, prolong the shelf life of stored food products, distribute via cold chain systems, and retain the natural colors and flavors of the food ingredients. Application of HPP in food industry ◦ Fruit puree was the first industrial application of HPP, which was developed in Japan in the early 1990s. Its numerous advantages have led to the extension of this technology to a wide variety of food and beverages. From the earlier HPP applications such as juices, avocado and meat products, to the latest trends such as ready-to-eat (RTE) meals, vegetable dips, baby food and pet food. ◦ Other application that can be used with HPP include: 1. Juices and beverages 2. Guacamole and avocado products 3. Meat products 4. Seafood products 5. Plant based and food products 6. Dairy products 7. Baby food and fruit purees 8. Ready to eat meals 1. Juices and beverages • Fresh-like, Premium Beverages. HPP is a non-thermal process that helps preserve the color, flavor and fresh attributes of beverages, which can be similar to a freshly squeezed juice. • Food Safety. Numerous scientific journals and validation studies state that HPP technology can achieve a 5-log reduction of relevant pathogenic microorganisms in juices, as required by the FDA in the United States. • Extended Shelf Life. Depending on the application and HPP recipe, it is possible to extend the shelf life of beverages anywhere between 6 weeks and 3 months, in some cases, even longer. • Clean Label. HPP is a non-thermal process and does not require preservatives or additives. Products can be marketed as a clean label product that is “cold-processed”, “non-thermally processed” or “natural”. • Functional and probiotic beverages. By using HPP, the nutritional and functional properties remain intact, maintaining its probiotic character and enzymatic activity. • Technology with no limitations on packaging. Previously with the In-Pack units, it was only possible to use flexible plastic packaging such as, bottles, bags, bag-in-box and containers. However, now with the new range of Hyperbaric Bulk Technology (it is a global innovation for high volume processing of HPP beverages. This new technology reduces steps, simplifying the process and offering the highest productivity in the world, along with the lowest processing cost and lowest energy consumption. In addition, it allows the use of any type of container after HPP processing, regardless of material, design, or size), it is possible to use glass, cardboard, or 100% biodegradable packaging. 2. Dairy products ◦ Milk: HPP raw milk is one of the applications that has recently found commercial success. It has an extended shelf life. ◦ Cheese: The high pressure processing of fresh cheese allows to inactivate pathogenic bacteria and improve the shelf life of the product for up to several weeks. In the case of aged cheeses, HPP can accelerate or modify the maturation. ◦ Yogurt: HPP inactivates contaminating microorganisms (yeasts and molds), keeping the enzymes alive (lactic acid bacteria) and preserving the probiotic properties. Yogurt can reach about 3 months of shelf life. ◦ Sandwich Fillings: Cheese-based and mixed with other ingredients, these products can be processed to improve the shelf life and destroy pathogens, while maintaining a high quality taste that is fresh and safe. 3. Meat products • Enhances Food Safety. HPP technology can eliminate pathogenic microorganisms and is the best food safety solution against foodborne pathogens such as Salmonella spp., E. Coli, and Listeria monocytogenes in meat products. • Premium Quality with an Extended Shelf Life. The inactivation of spoilage microorganisms (lactic acid bacteria, yeasts and molds) significantly increases the shelf life of the product and guarantees high sensorial quality. • Clean Label. The use of HPP makes it possible to design formulations that are free of preservatives and additives. This meets the demand of products with a clean label and reduced amounts of sodium. • No Cross-Contamination. Because HPP is applied after packaging, it elimates the risk of cross- contamination during handling and slicing. • Variety of Applications. The pressure applied during the process is transmitted uniformly and instantaneously throughout the entire product, so the effectiveness is the same regardless of the product format. • Various Packaging Options. There are a various packaging options including, vacuum, in skin, and MAP trays or bags. The type of packaging can modify the productivity of the line but the effectiveness of the technology on the product is guaranteed. 4. Ready to eat meals • Due to the busy schedule consumers have, HPP ready-to-eat (RTE) meals have increased in popularity. This application offers practical meals that are similar to those made fresh at home. It multiplies up to 3 times the shelf life while refrigerated, maintaining all the fresh properties of the food. • Premium Quality. HPP is a non-thermal process that maintains the color, flavor and nutritional value of products. • Minimum Previous Cooking. Before the HPP treatment, the ready-to-eat meal requires only minimal cooking to achieve optimal flavor. • Food Safety. HPP inactivates foodborne pathogens and spoilage microorganisms such as Salmonella spp.,E. Coli, molds and yeasts. In addition, recontamination is avoided since products are processed in their final packaging. • Variety of Products. HPP can be applied to a range of RTE products that are prepared with ingredients such as meat, fish, vegetables, rice, and pasta. 5. Pharma and cosmetics • In recent years, interest in HPP has been increasing for the development and preservation of pharmaceutical products, as well as the research of new vaccines and drugs. • In cosmetic products, HPP offers the possibility of developing products with ingredients that are natural, organic and ecological. • Natural and Organic Cosmetics. Development of products without chemical additives. • Cream and lotions are susceptible to microbiological deterioration. HPP technology is presented as an alternative for its preservation. • Molecular Modification. Structural changes in proteins and enzymes caused by the HPP process open the door to the creation of molecules with new functionalities or enzymatic synthesis of new compounds.