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Conservative
Transpositional
site-specific
recombination
recombination
Homologous Recombination
• Double-strand break
• Strand invasion
• Holliday junction
• Resolution either regenerates original
duplexes or generates crossover
Holliday Junction
• Isomerization
• Resolution by cutting strands
Gene Conversion
• Involves excision of the transposon from the old DNA location and
insertion to a new site.
• Or, one copy of the transposon stays at the old location and another
copy is inserted into the new DNA site.
Three classes of Transposable Elements
• DNA transposons
• Viral-like retrotransposons
- long terminal repeat (LTR) retrotransposons
• Non-viral retrotransposons
- poly A retrotransposons.
DNA transposition by a Cut-and-Paste Mechanism
• Transposase bind to the terminal inverted repeats at the end of the
transposon.
• Transposase brings the two ends of the tranposon DNa together to
generate a stable protein-DNA complex (synaptic complex or
transpososome which is essential to coordinate DNA cleavage and joining
reactions on the two ends of the transposon’s DNA).
• Transposase cleaves DNA such that transposon sequence terminates with
free 3’-OH groups at each end of the element’s DNA.
• 3’-OH ends of the transposon DNA attack the DNA phosphodiester bonds at
the site of new insertion.
• Gap repair by DNA polymerase.
DNA transposition by a Replicative Mechanism
• Element DNA is duplicated during each round of transposition.
• Mechanism:
i) Assembly of the transposase protein on the two ends of the transposon
to generate a transpososome.
ii) DNA cleavage at the ends of the transposon DNA. Transposase
introduces a nick into DNA at each of the junctions between the transposon
sequence and the flanking host DNA.
iii) The 3’OH ends of transposon DNA are then joined to the target DNA
site by the DNA strand transfer reaction whereas 5’ ends of the transposon
sequence remain joined to the old flanking DNA.
iv) The 3’-OH end in the cleaved target DNA serves as a primer for DNA
synthesis. Replication proceeds through the transposon sequence and stops
at the second fork.
DNA-Only Transposons
• RNA genome
• Reverse transcription (RNA→DNA)
• Integration of DNA
• Transcription (DNA→RNA)
Retroviral-Like
Retrotransposons
• Retroviral trans-position
mechanism
• Integration of DNA via
RNA intermediate
Nonretroviral Retrotransposons