SUMMARY Cokorda Javandira. 116040217011015. The Potential of Bacillus sp. and Pseudomonas sp.

as Biological Control Agents Against Corn Leaf Blight Disease Caused b Pantoea sp. Supervised by Prof. Dr. Ir. Abdu !a"ief Abadi# $S. and !u%&an 'ura"a Aini# SP#. $.Si#. P(.D. Corn )Zea mays !.* is +ide y used for sour,e of ,arbo(ydra"e# food indus"ries# and food ives"o,k ,o&&odi"ies in Indonesia-s. .ne of ne+ bio"i, ,ons"rain"s of ,orn produ,"ion is eaf b i/(" disease ,aused by Pantoea sp. +(i,( is need "o be ,on"ro ed. .ne a "erna"ive ,on"ro &easures is "(e used of ba,"eria Bacillus sp. and Pseudomonas sp. for bio o/i,a ,on"ro . 0(e ai& of "(is s"udy is "o e u,ida"e "(e po"en"ia of Bacillus sp. and Pseudomonas sp. s"rains as bio o/i,a ,on"ro a/ains" eaf b i/(" disease ,aused by Pantoea sp. 0(e resear,( +as done in "(e p an" pa"(o o/y abora"ory and / ass (ouse of A/ri,u "ure 1a,u "y# 2niversi"y of 3ra+i4aya fro& Au/us" 2012 un"i Apri 2015. Co&p e"e y 6ando&i7ed Desi/n +as used in "(is s"udy# ,onsis"s of 8 "rea"&en"s. In "(is s"udy# severa assays +ere ,ondu,"ed# in, udin/ an"a/onis"i,s assays on a/ar p a"e# ,(ara,"eri7a"ion of "(e "ype of an"ibiosis produ,ed by an"a/onis"s and po" e9peri&en" in ,on"ro in/ eaf b i/(" disease ) Pantoea sp.* usin/ by Bacillus sp. and Pseudomonas sp. 0(e da"a fro& "(e observa"ions of an"a/onis"i,s assay on a/ar p a"e# in"ensi"y of eaf b i/(" disease on ,orn# and "(e es"i&a"ion of ba,"eria popu a"ion on eaves +ere ana y7ed by ana ysis of varian,e )A:.;A* usin/ $i,rosof" <9,e sof"+are and ,on"inued by Dun,an &u "ip e ran/e "es" )D$60* on 0.01 ,onfiden,e eve . 0(e resu "s s(o+ed "(a" a ba,"eria s"rains of Bacillus sp. and Pseudomonas sp. (ave po"en"ia in in(ibi"in/ "(e /ro+"( of Pantoea sp. by s(o+in/ "(e , ear 7one on "(e a/ar p a"e. 0(e an"ibiosis "ypes are ba,"eri,ide or ba,"erios"a"i,. .n po" e9peri&en" a ba,"eria s"rains s(o+ed "(e redu,"ion of "(e disease in,iden,e a" "(e sa&e eve ,o&pared "o s"rep"o&y,in sup(a"e. A ba,"eria s"rains as +e as ba,"eri,ide redu,ed "(e disease in,iden,e a" 18=24> ,o&pared "o ,on"ro )a%uades "rea"&en" on y*. 0(e popu a"ion of Bacillus sp. and Pseudomonas sp +ere (i/( a" "(e avera/e of 10 &i ion ,e s per ,& 2 a" seven days af"er ino,u a"ion# su//es"ed "(a" a ba,"eria an"a/onis"s (ave (i/( epip(y"i, fi"ness +(en app ied in "(e ,orn eaves. 0(e resu "s su//es" "(a" a ba,"eria s"rains are po"en"ia as bio o/i,a ,on"ro a/en" a/ains" eaf b i/(" disease on ,orn eaf ,aused by Pantoea sp.

ii