Examining Cultural Characteristics

Plates- streak plates for isolated colonies

Describe:
1. Margin curled (concentric even or irregular rings), entire (smooth,
even), lamentous (hair!, thread-like appearance), lobate (lobes "ith
deeper indentations than undulate), undulate (regular "ave-like
pattern), serrate (sa" toothed pattern)
2. Surface concentric (ring-like pattern), rugose ("rinkled), contoured
(smooth fold-like pattern), radiate (spoke-like pattern), smooth
3. Whole-colony form circular (round), granular, punctiform (ver!
minute, pin-point), lamentous (thread-like appearance), irregular
(var!ing, non-uniform shape), rhi#oid (root-like appearance)
4. Elevation convex (curving out"ard), pulvinate (higher dome than
convex), umbonate (rounded double terraced surface), $at (level or
slightl! above agar), raised (pro%ects above agar, "ith $at or even
surface), subsurface (pour plates onl!, these are spindle-shaped
colonies)
5. Chromogenesis (color)
Broths- inoculate using loop transfer
Describe:
1. Surface (do not mix)- no gro"th (&), ring, membrane (thin lm),
pellicle (thick lm), $occulent ($akes), granular
2. Middle (do not mix)- no gro"th (&), ne turbidit!, granular,
$occulent
3. Bottom or sediment (do this last and mix)- no gro"th (&), ne
turbidit!, granular, $occulent, viscid (string! or rop!)
Slants- stroke slant "ith needle
Describe:
1. Form- arborescent (tree-like or branched), beaded (separate
colonies), echinulate (margins are tooth-like or serrated, usuall!
pointed at top), liform (even or uniform gro"th at point of
inoculation), rhi#oid (root-like), spreading (highl! motile forms take
over slant)
2. Density- opa'ue (thick gro"th, no light "ill get through), translucent
(moderate gro"th, some light "ill get through), transparent (thin
gro"th, light penetrates easil!)
Gelatin Deeps- stab deep "ith needle
Describe:
1. (fter placing the tube in an ice bath for ) minutes, look to see if there
is li'uefaction* +he result sheet "ill have a ,pos- for li'uefaction or a
,neg- for no li'uefaction* Do not turn the tube upside do"n* (n! li'uid
in the tube should be interpreted as a positive* .i'uefaction means
that the organism "as capable of protein (gelatin) h!drol!sis*