Set No.

Code No: R05222302

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007

INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks

1. Give a brief account on methods for reporting analytical data?

[16]

2. (a) Give the principle involved in SEM ?

(b) Which aspects of morphological study the SEM is used for?

[8 2 = 16]

3. (a) Explain the technique density gradient centrifugation?

(b) How important is the technique in modern biology?

[8 2 = 16]

4. (a) Explain:
i. Rayleigh scattering
ii. Raman scattering.
(b) Give the basic design of a spectrophotometer.

[8 2 = 16]

5. (a) Give the principle involved in circular dichroism

(b) Give the components of circular dichroism apparatus.

[8 2 = 16]

6. Write short notes on:

(a) overtones
(b) vibrational coupling
(c) fermi resonance.

[5+5+6]

7. Write brief notes on:

(a) nuclear overhauser effect
(b) chemical shift.

[8 2 = 16]

8. (a) Discuss the principle & working of electron spin resonance?

(b) give important applications of ESR.

1 of 1

[8 2 = 16]

Set No. 2

Code No: R05222302

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007

INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks

1. Give a brief account on methods for reporting analytical data?

[16]

2. (a) Draw a neat labeled diagram of phase contrast microscope.

(b) Describe the function of every part involved in it.

[8 2 = 16]

3. Write a short notes on two methods of analytical centrifugation :

(a) Schlieren system
(b) Rayleigh interference optical system.

[8 2 = 16]

4. Differentiate between the following terms:

(a) Band spectra & Line spectra.
(b) Fluorescence & Phosphoroscence
(c) Molar absorptivity & Absorptivity
(d) Stokes shift & Bathochromic shift.

[4 4 = 16]

5. (a) What is quenching? Discuss the theory of fluorescence & phosphorescence.

(b) Differentiate the resonance fluorescence & sensitized fluresence. [8 2 = 16]
6. Write short notes on:
(a) overtones
(b) vibrational coupling
(c) fermi resonance.

[5+5+6]

7. Write brief notes on:

(a) nuclear overhauser effect
(b) chemical shift.

[8 2 = 16]

8. (a) Why electron spin resonance is also called electron para magnetic resonance?
(b) Calculate the ESR frequency of an unpaired electron in a magnetic field 0.33 .
Given for free electron g = 2& = 9.273 1024 JT 1 .
[8 2 = 16]

1 of 1

Set No. 3

Code No: R05222302

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007

INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks

1. Give a brief account on following analytical methods:

(a) Gravimetric
(b) Volumetric
(c) Separation methods.

[16]

2. Draw a neat labeled diagram of a compound microscope & give the principle underlaying in magnification of an object.
[16]
3. (a) Describe about an analytical centrifuge equipment with a supportive block
diagram of it?
(b) How different is analytical centrifuge from ultracentrifuges?

[8 2 = 16]

4. (a) Calculate the frequency in hertz of

i. an x-ray beam with a wave length of 2.65 A0 .
ii. the line at 694.3nm produced by a ruby laser.
iii. an infra-red absorption peak at 19.6 m.
(b) Express the following absorbances in terms of % transmittance:
i. 0.064
ii. 0.765
iii. 0.318.

[8 2 = 16]

5. (a) Why is source modulation employed in atomic absorption spectroscopy?

(b) In a hydrogen/ oxygen flame, an atomic absorption peak for iron decreased in
the presence of large concentration of sulphate ion.
i. suggest an explanation for this observation.
ii. Suggest three possible methods for overcoming the potential interference
of sulphate in a qualitative determination of iron.
[8 2 = 16]
6. (a) Explain the origin of molecular spectra.
(b) Differentiate clearly between ultraviolet & infra red spectra.
(c) The absorption of ultraviolet & visible radiation can be conveniently studied
together,but infra red absorption studies are made separately. Why? [5+5+6]
7. Write brief notes on:
1 of 2

Set No. 3

Code No: R05222302

(a) nuclear overhauser effect
(b) chemical shift.

[8 2 = 16]

8. (a) Discuss the principle & working of electron spin resonance?

(b) give important applications of ESR.

2 of 2

[8 2 = 16]

Set No. 4

Code No: R05222302

II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2007

INSTRUMENTAL METHODS OF ANALYSIS
(Bio-Technology)
Time: 3 hours
Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks

1. Write short note on:

(a) Problems in analysis
(b) Methods of analysis.

[8 2 = 16]

2. Describe how do flow cytometry helps in detecting:

(a) the presence of heterogenous microbial population?
(b) Cell sorting based on size & shape of the cell?

[8 2 = 16]

3. How are the analysis of sub cellular fractions are carried by the centrifugation
technique.Explain?
[16]
4. What are the different regions of the electromagnetic spectrum & where are they
used for?
[16]
5. (a) Define fluorescence. Derive an expression relating intensity of fluorescence &
concentration.
(b) Write a brief note on :
i. Triplet state
ii. Doublet state
iii. Singlet state.

[8 2 = 16]

6. Explain the following:

(a) The cause of absorption in UV & IR regions.
(b) The information gained from UV & IR spectra about the nature of organic
compounds.
(c) The region of greatest intrest to organic chemist for UV & IR spectra. [5+5+6]
7. (a) Explain the principle involved in CMR spectra?
(b) Why it is not possible to determine relative ratio of carbon atoms in a compound by integration of peak areas in 13C NMR as in PMR?. [8 2 = 16]
8. (a) Discuss the principle & working of electron spin resonance?
(b) give important applications of ESR.

1 of 1

[8 2 = 16]