--.

}:'u'
DepartInent:'~f Bioclle~i,cal- Engineering an'd iBiotcchnology
X f,." "'::; ,',:t".f':':';.:I' . , ' 'I

.,!;
J.
. .MaJor Test!::,': Semester I (200,1-2008)
]: "BEt)~~l(~~8i~8.§~~5,-S:
E~~i ~,,'\~~~ng)', .
"
.'
j' .. ):;i,l~lf';ff' ,. ' ", Date:3'Q.U,,-200,7
, Timc:IO:30 - 12:30 AM
",
'f: .:1' i Venue:]]-3.18'
.,.,f.,".
l,..r;.'!",,:
<',7':' " , , ' MI'ar<.s:.' 50
A11sw.t.r
';
~"~~h1:
~ut.~.t:~,\.~
.t ':; .,' '.~;:,}:J:-:':':';;:.;;;;;,;
to £? 0 m~o~
,', ,
. ,

1.
K.L. Phillips,
, earr~~d'i{?~<}{e~11~el1tation
, .., , ., .. -.~" ,.' in. a 3'1 diameter HRF using T. II/ilis.
Fermentation conditions' Y,lereas below:
:;::\" .~
"
", ' , .;; ,.; , , /)
'vV orking volume in the fennenter = '0,5 -I I
,.~~(O ji-t
Air flow rate = 150 cc/mi 11 . 1
Tcmperature = 30°C
Fermenter rotation speed =
500 rpm
i'
During fermentation . ",,--
the following data 'were collected:
J~ ,
~~ 0-{ c;
\..--
U)~
;', .'

/
~
}.~ 0'1'
,JJ1/1'
tJV(...
--------- -- -- --- ~ ----'"- ---------------------------------------
Ti 11.1'
~ % ftin exit air offermentcr

.-

~
- - -- -- --. - - ---'._- - --- '::'_~;,-::'~_':'_-'-' --'--;-- -- -..:- -- -- -- - -- - - ---- - - - -- -- ---- - - -- -- - -- --
 <,.
7 "' . ..
I

..

",""
,.
" '.' '" :"', ,'"
I,.. .. " ""r';
-f

---
t--
--.---

r-- ..'.

0l!+

~ ...te-- -....
 '- ~---, ;..3-
~

"
,.'"

I-" -
1
11
)(

I
X

I
ij
I
I

"',

r
'C
,.-..---..- . --, L~.-'__~,;. ' ',~.L: ~ ':':"..: ~..,L,:~~ ,C .'..(8)
~
Q.5. List the factors affecting the volumetric oxygen transfer coefficient
l~
1
(KLa).(4-Marks) \
IJ
A stTainof Saccharomyces cerevisiae is grown in a batch culture in a
14L fermentor (working volume =; lOL). The dissolvedoxygen is
maintained constant at 25% saturation value by aut<;)matic
manipulation of aeration and agitation rates. ,How m~ch maximum
cell mass, (glL) can the fermentor support if the maximum KLa is 320
h-I? ...t\ssume that the specific oxygen uptake rate for the yeast is 3
milli-moles O2/ (g-cell -h), and the oxygen saturation level of
I Dissolved: oX'Jgen in the mediu'D is 8 mg oxygeniT...J'Specify
W assumptions made, if any. (6-Marks)
I

Cc'(/~Cl-)
(\
Ku..

I. tAL0'- ~ ('"

or f0) 1!--
[,-t) ~ +I/-Mtjt (I~')
J 7
\t'L
. . Jr'f~"V
I
il ,'L- ~r~ ' Vf.. ~ ..
~

r
".<""