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Dotes Biochemistry
Dotes Biochemistry
Dominus Testimonium
Please use a dark pencil/pen to complete the test. Read the instructions at the
begining carefully. This is an Sample Test Paper for DOTES Entry Level Examination
toward DOTES Cert. Level A. This series contains questions in Biochemistry. This is
an entry level examination to be eligible to appear for DOTE Certification Levels.
Please Note: There is a Negative Marking of (-1) for each incorrect response.
1. In fatty acid synthesis, the enzyme required for priming acetyl-ACP with
malonyl-ACP for chain extension
A 3-ketoacyl-ACP reductase
B 3-ketoacyl-ACP synthase
C Methylcrotonyl-CoA carboxylase
B 6-phosphogluconate
dehydrogenase
C scaly dermatitis
B glossitis
D dementia
4. Match the following Vitamins with their daily recommended dietary intake as
supplement:
A.
B.
C.
D.
E.
Thiamine
Cobalamin (Vit.B12)
Niacin
Pantothenic acid (B5)
Biotin
A.
B.
C.
D.
E.
5 mg
1.1-1.2 mg
14-16 mg
2.4 mg
30 g
C zeaxanthin
B -cryptoxanthin
D lycopene
-1-
Biochemistry
6. The enzyme that catalyzes reductive amination of -ketoglutarate in prokaryotes
and plants
A glutamate synthase
C glutamate dehydrogenase
B glutaminase
D glutamine synthetase
7. Arrange the following substrates of the glycolytic pathway in their correct order of
events/reactions: (1 5)
A.
B.
C.
D.
E.
1,3-Bisphosphoglycerate
Fructose 1,6-bisphosphate
Phosphoenolpyruvate
Glyceraldehyde 3-phosphate
Glucose 6-phosphate
D apoenzyme + coenzyme
catalase
A. manganese
B.
C.
D.
E.
cytochrome
hexokinase
arginase
nitrate reductase
B.
C.
D.
E.
magnesium
molybdenum
ferrous or ferric
cupric
C lactate dehydrogenase
B fumarase
D aminoacyl-tRNA synthetase
12. The pyruvate dehydrogenase multienzyme complex require all of the following
cofactors except:
A thiamine pyrophosphate (TPP)
B cobalt
D magnesium
-2-
Biochemistry
13. The enzyme that converts glutamine to glutamate and NH4+ in liver mitochondria
A glutamine synthetase
C glutamate dehydrogenase
B glutamate synthase
D glutaminase
C -ketoglutarate
B 3-phosphoglycerate
D 3-phosphohydroxypyruvate
C aspartate
B pyruvate
D glutamine
16. Which of the following substances is/are responsible for brain edema due to
astrocyte swelling in hepatic encephalopathy?
A glutamine
C glutamate
B ammonia
17. Match the following enzymes with their correct classification subgroup:
A.
B.
C.
D.
E.
phospholipase C
lactate dehydrogenase
phosphoglucomutase
fumarase
UDP-glucose pyrophosphorylase
A.
B.
C.
D.
E.
Hydrolase
Transferases
Oxidoreductase
Isomerase
Lyases
C glutamate synthase
B glutaminase
D glutamate dehydrogenase
C kainic acid
B kynurenic acid
D glutamic acid
C aspartate transcarbamylase
B histidine decarboxylase
D Uracil-DNA glycosylase
-3-
Biochemistry
21. Which of the following is not true about Vitamin E?
A regulation of prostaglandin
synthesis
C lutein
B lycopene
D -cryptoxanthin
C Pyridoxine
B Vitamin E
D Biotin
C tryptophan
B alanine
D tyrosine
C cyanocobalamin
B adenosylcobalamin
D hydroxocobalamin
C Vitamin E
B Vitamin D
D Vitamin C
27. Hormones that act through Ca2+ and phosphatidylinositol bisphosphate (PIP)
system
A oxytocin
C glucagon
D insulin
28. Enzyme that catalyzes the synthesis of fumarate from succinate and FAD
A succinyl dehydrogenase
B -ketoglutarate dehydrogenase
D succinate dehydrogenase
-4-
Biochemistry
30. Which of the following substrate inhibits carnitine acytransferase I?
A acetyl CoA
C palmitoyl CoA
B malonyl CoA
D succinyl CoA
31. Pick the incorrect statements about the tricarboxylic acid cycle (TCA cycle)
A involves oxidation of acetyl CoA
32. Formation of methylmalonyl CoA from propionyl CoA require which vitamin as a
cofactor?
A Thiamine
C Vitamin B12
B Pyridoxine
D Vitamin B7
C boiled milk
B yoghurt
D raw milk
C Pyridoxine
B Folic acid
D Biotin
C molybdenum
B iron atoms
-5-
A.
B.
C.
D.
Riboflavin
Niacin
Thiamine
Pantothenic acid
Biochemistry
37. Regarding -subunits of voltage gated sodium channels (Nav) true is:
A four repeat domains with six
membrane-spanning segments
39. Which of the following enzymes require lipoic acid for its activity?
A pyruvate dehydrogenase
C -ketoacid dehydrogenase
D superoxide dismutase
C 2-furoyl-CoA dehydrogenase
2-furoyl-CoA dehydrogenase
B tyrosinase
D uridine monophosphate
synthetase
41. The gene that codes for enzyme Uridine monophosphate synthetase is located on
the
A short arm of chromosome 3 (3p13)
B short arm of chromosome 11 (3
p11)
C intestine
B kidney
D liver
C Thiamine
B Riboflavin
D Biotin
44. Wild type strain of bacteria that do not require any growth factors:
A Lactobacillus
C Haemophilus influenzae
B E. coli
D Neisseria meningitidis
-6-
Biochemistry
45. Synthesis of Porphyrin ring requires all of the following except:
A acetyl CoA
C succinyl CoA
B glycine
D -Aminolevulinic acid
C domoic acid
B okadaic acid
D conotoxin
C Retinol
B Retinal
D Retinene
C deficiency of cosynthase
B increased synthesis of
-aminolevulinate
C phylloquinone
B Vitamin K1
D phytomenadione
C Vitamin B12
B Vitamin B1
D Vitamin B7
-7-