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  • 12-4-15 Presentation

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    Sam Boland
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    The document summarizes an experiment testing the effects of benzyl alcohol on cell migration using PDMS columns. It found that benzyl alcohol only increased total migrated cells by 31 cells and average migrated cells per well by 10 cells compared to the control, a difference of only 10-12%. Issues with inconsistent image areas and not capturing the entire migration region were noted. A new experiment was proposed using larger diameter PDMS columns taped to the bottom of well plates to better define boundaries and complete the experiment.

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    The document summarizes an experiment testing the effects of benzyl alcohol on cell migration using PDMS columns. It found that benzyl alcohol only increased total migrated cells by 31 cells and average migrated cells per well by 10 cells compared to the control, a difference of only 10-12%. Issues with inconsistent image areas and not capturing the entire migration region were noted. A new experiment was proposed using larger diameter PDMS columns taped to the bottom of well plates to better define boundaries and complete the experiment.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    2 views9 pages

    12-4-15 Presentation

    Uploaded by

    Sam Boland
    The document summarizes an experiment testing the effects of benzyl alcohol on cell migration using PDMS columns. It found that benzyl alcohol only increased total migrated cells by 31 cells and average migrated cells per well by 10 cells compared to the control, a difference of only 10-12%. Issues with inconsistent image areas and not capturing the entire migration region were noted. A new experiment was proposed using larger diameter PDMS columns taped to the bottom of well plates to better define boundaries and complete the experiment.

    Copyright:

    © All Rights Reserved
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    of 9

    Manipulating Cell

    Migration Using
    Amphiphiles
    11/20/15 Meeting

    Last Time
    Images taken from
    PDMS column
    experiment
    At first glance,
    experiment seemed
    to support hypothesis

    Benzyl Alcohol
    FIJI used to stitch together images. Then cells manually counted by
    hand.
    Total number of migrated cells: 266
    Average number of migrated cells: 89

    Control
    Total number of cells migrated: 297
    Average number of cells migrated: 99

    Comparison
    Only 31 more cells migrated in total in the control
    This is only an increase of about 10-12%

    On average, only 10 more cells migrated per well in the


    control model.

    Validity Issues
    There are cells here
    that could not be
    captured in the image
    The areas of each image are not
    consistent and there is not a
    good way to measure
    Entire area covered by column
    would need to be imaged which
    would be very time consuming

    New Experiment

    Originally tried just using small pipet tips not effective


    Filled pipets with PDMS, let small bit drip out bottom
    New columns taped to bottom of well plate

    Problems
    Boundaries not very well-defined
    Some wells unusable- not enough to complete
    experiment

    Where to go from here


    Manufactured cell migration assembly too
    expensive ($255 for one kit)
    Two options:
    1. Repeat last experiment but with more columns
    2. Use larger diameter columns

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