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  • DiI Liposome Final

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    Sam Boland
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    This document analyzes the use of DiI liposomes with varying concentrations of PEG to determine liposome diameter using time-correlated single photon counting. Higher PEG concentrations led to better fitting of fluorescence correlation spectroscopy curves and indicated smaller, more stable liposomes. While liposome diameter did not change over time, anionic liposomes had smaller diameters than normal liposomes.

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    This document analyzes the use of DiI liposomes with varying concentrations of PEG to determine liposome diameter using time-correlated single photon counting. Higher PEG concentrations led to better fitting of fluorescence correlation spectroscopy curves and indicated smaller, more stable liposomes. While liposome diameter did not change over time, anionic liposomes had smaller diameters than normal liposomes.

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    © All Rights Reserved
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    11 views6 pages

    DiI Liposome Final

    Uploaded by

    Sam Boland
    This document analyzes the use of DiI liposomes with varying concentrations of PEG to determine liposome diameter using time-correlated single photon counting. Higher PEG concentrations led to better fitting of fluorescence correlation spectroscopy curves and indicated smaller, more stable liposomes. While liposome diameter did not change over time, anionic liposomes had smaller diameters than normal liposomes.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 6

    Analysis of DiI

    Liposome Diameter
    using TCSPC
    Sam Boland
    11/11/16

    Higher concentrations of PEG led to more


    successful fitting of FCS curves

    Quality of curve fit increases with


    PEG concentration
    A: 50nm Bead
    B: 1% PEG Liposome
    C: 2% PEG Liposome
    D: 5% PEG Liposome
    E: 10% PEG Liposome
    F: 15% PEG Liposome

    Multi-Channel Scaling (MCS) curve indicates


    degradation of low percent PEG Liposomes
    MCS for 10% PEG Liposome

    Thin peaks
    indicate
    photon
    absorption
    by
    liposome

    MCS for 1% PEG Liposome

    Intermittent
    thick peaks
    indicate
    photons are
    being
    absorbed by
    large masses
    of lipids

    There is no observable trend for


    liposome diameter over time

    Anionic liposomes have smaller


    diameters than normal liposomes

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