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  • 10-16-15 Presentation

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    Sam Boland
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    The document summarizes a meeting about a project measuring how amphiphiles affect cell migration using a razor blade assay. Preliminary results found that the control on average saw 14 cells migrate, ethanol saw 16 cells migrate, and benzyl alcohol saw either 38 cells or 82 cells migrate after removing an outlier, though the latter number seems too high. Improvements discussed included collecting more data, fixing and staining cells correctly, creating cleaner razor wounds, and considering computer aided counting or also testing vitamin E.

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    The document summarizes a meeting about a project measuring how amphiphiles affect cell migration using a razor blade assay. Preliminary results found that the control on average saw 14 cells migrate, ethanol saw 16 cells migrate, and benzyl alcohol saw either 38 cells or 82 cells migrate after removing an outlier, though the latter number seems too high. Improvements discussed included collecting more data, fixing and staining cells correctly, creating cleaner razor wounds, and considering computer aided counting or also testing vitamin E.

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    3 views6 pages

    10-16-15 Presentation

    Uploaded by

    Sam Boland
    The document summarizes a meeting about a project measuring how amphiphiles affect cell migration using a razor blade assay. Preliminary results found that the control on average saw 14 cells migrate, ethanol saw 16 cells migrate, and benzyl alcohol saw either 38 cells or 82 cells migrate after removing an outlier, though the latter number seems too high. Improvements discussed included collecting more data, fixing and staining cells correctly, creating cleaner razor wounds, and considering computer aided counting or also testing vitamin E.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    of 6

    Manipulating Cell

    Migration Using
    Amphiphiles
    10/16/15 Meeting

    Project Overview
    Extension of previous membrane paper and Seoyoungs
    current work
    Measuring how many cells cross a line in 24 hours
    Razor blade assay

    Preliminary Results: Control


    Average number of migrated cells: 14

    Ethanol
    Average number of migrated cells: 16
    Data inconsistent

    Benzyl Alcohol
    Average Number of Migrated Cells: 38
    With outlier removed: 82
    Number seems very high. Possible cells were not removed.

    Improvements
    Collect more data
    Fix and stain correctly
    Create razor wound more effectively, remove debris

    Computer aided counting?


    Test Vitamin E as well
    Change in shape?

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