Manipulating Cell

Migration Using
Amphiphiles
10/23/15 Meeting

Last time
Cell migration affected by amphiphiles
Preliminary results

Encountered problems with fixing and staining

Control
DAPI

Phase

DAPI

2 of 4 samples pictured
Total # of migrated cells: 124
Mean: 31
More cells migrated than in preliminary results

Phase

Ethanol

2 of 4 samples shown
Total # of migrated cells: 118
Mean: 30
More than in preliminary results
DAPI

Phase

DAPI

Phase

Benzyl Alcohol: Razor Assay

DAPI

Phase

2 of 4 samples
Total # of migrated cells: 67
Mean: 17
Less than preliminary results

DAPI

Phase

*DAPI did not appear to work*

Benzyl Alcohol: Pipet Assay

2 of 4 samples
*Again, DAPI not effective*
Total # of migrated cells: 92
Mean: 23
Surprisingly different than razor assay
Pre-migration

Post-Migration

DAPI

Analysis
Data conforms more to expectations
Control and ethanol very similar
BA less than control

Better staining would result in more accurate counting

Pipet method overcomes cutting plate but adds more
intricacy and difficulty to process due to pre-imaging
and less defined borders.